##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	157394
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24445658665515	33.0	31.0	34.0	30.0	34.0
2	32.334790398617486	34.0	31.0	34.0	30.0	34.0
3	32.298759800246515	34.0	31.0	34.0	30.0	34.0
4	35.89587277786955	37.0	35.0	37.0	35.0	37.0
5	35.98076165546336	37.0	35.0	37.0	35.0	37.0
6	35.968645564633974	37.0	35.0	37.0	35.0	37.0
7	36.149560974370054	37.0	36.0	37.0	35.0	37.0
8	35.981676556920846	37.0	35.0	37.0	35.0	37.0
9	37.90237239030713	39.0	38.0	39.0	35.0	39.0
10	37.596439508494605	39.0	37.0	39.0	35.0	39.0
11	37.54149459318653	39.0	37.0	39.0	35.0	39.0
12	37.36604953174835	39.0	37.0	39.0	35.0	39.0
13	37.28682160692276	39.0	37.0	39.0	34.0	39.0
14	38.457952653849574	40.0	38.0	41.0	34.0	41.0
15	38.575085454337525	40.0	38.0	41.0	35.0	41.0
16	38.59310393026418	40.0	38.0	41.0	35.0	41.0
17	38.539868101706546	40.0	38.0	41.0	35.0	41.0
18	38.37127209423485	40.0	37.0	41.0	35.0	41.0
19	38.20602437195827	40.0	37.0	41.0	34.0	41.0
20	38.0238446192358	40.0	35.0	41.0	34.0	41.0
21	37.88451275143906	40.0	35.0	41.0	34.0	41.0
22	37.91504123410041	40.0	35.0	41.0	34.0	41.0
23	37.86668487998272	40.0	35.0	41.0	34.0	41.0
24	37.80402683710942	40.0	35.0	41.0	34.0	41.0
25	37.59686519181163	39.0	35.0	41.0	33.0	41.0
26	37.64558369442291	39.0	35.0	41.0	34.0	41.0
27	37.633054627241194	39.0	35.0	41.0	34.0	41.0
28	37.544747576146484	39.0	35.0	41.0	33.0	41.0
29	37.48203870541444	39.0	35.0	41.0	33.0	41.0
30	37.25165508215053	39.0	35.0	41.0	33.0	41.0
31	37.13860757080956	39.0	35.0	41.0	33.0	41.0
32	36.92591839587278	39.0	35.0	41.0	33.0	41.0
33	36.68357751883808	39.0	35.0	41.0	32.0	41.0
34	36.45645323201647	39.0	35.0	41.0	31.0	41.0
35	36.287202815863374	39.0	35.0	41.0	30.0	41.0
36	36.145882308093064	39.0	35.0	41.0	30.0	41.0
37	36.06653366710294	39.0	35.0	41.0	30.0	41.0
38	35.91394208165495	39.0	35.0	41.0	29.0	41.0
39	35.8455722581547	39.0	35.0	41.0	29.0	41.0
40	35.7292908243008	39.0	35.0	41.0	28.0	41.0
41	35.66302400345629	39.0	35.0	41.0	28.0	41.0
42	35.54167249069215	38.0	35.0	41.0	27.0	41.0
43	35.40969160196704	38.0	35.0	41.0	26.0	41.0
44	35.40459610912741	38.0	35.0	41.0	27.0	41.0
45	35.37070028082392	38.0	35.0	41.0	26.0	41.0
46	35.283676633162635	38.0	35.0	40.0	26.0	41.0
47	35.26491479980177	38.0	35.0	40.0	27.0	41.0
48	35.21797527224672	38.0	35.0	40.0	26.0	41.0
49	35.16068592195382	37.0	35.0	40.0	27.0	41.0
50	35.06174949489815	37.0	35.0	40.0	26.0	41.0
51	34.26264025312274	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	1.0
12	4.0
13	3.0
14	5.0
15	9.0
16	23.0
17	22.0
18	49.0
19	88.0
20	156.0
21	245.0
22	377.0
23	598.0
24	918.0
25	1439.0
26	2238.0
27	2536.0
28	2530.0
29	2323.0
30	2503.0
31	2804.0
32	3347.0
33	4767.0
34	9498.0
35	15247.0
36	12054.0
37	15961.0
38	27856.0
39	49731.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.39981193692263	20.68947990393535	23.166067321498915	17.74464083764311
2	35.802508354829286	24.291269044563325	24.944407029492865	14.961815571114528
3	31.17780855687002	22.582182294115405	31.09140119699607	15.1486079520185
4	27.987089723877656	24.1889779788302	30.197466231241343	17.626466066050803
5	23.52758046685388	28.797158722695908	28.87340051081998	18.801860299630228
6	24.66993659224621	32.07746165673406	28.492826918433995	14.75977483258574
7	73.2728058248726	6.6768745949655015	15.156232130830908	4.8940874493309785
8	73.10634458746839	6.567594698654331	15.046316886285375	5.279743827591903
9	66.68106789331233	8.502865420536997	17.432049506334423	7.384017179816257
10	38.8737817197606	22.77342211265995	23.413217784667776	14.939578382911675
11	30.366468861583034	22.720688209207466	27.354282882447873	19.55856004676163
12	26.48131440842726	20.76762773676252	33.79036049658818	18.960697358222042
13	23.983760499129573	23.380815024715044	34.053394665616224	18.582029810539154
14	19.252322197796612	27.793308512395644	32.9847389354105	19.969630354397243
15	18.744678958537175	22.718146816269996	38.81278828926135	19.724385935931483
16	20.79304166613721	22.149510146511304	35.759304674892306	21.298143512459177
17	20.168494351754195	22.798200693800272	32.27823169879411	24.75507325565142
18	21.226984510210045	22.95767310062645	34.2166791618486	21.59866322731489
19	25.017472076445102	23.248662591966657	30.62632628943924	21.107539042149
20	23.478658652807603	25.458403751095975	31.07361144643379	19.98932614966263
21	22.52118886361615	25.526386012173276	31.362059544836523	20.590365579374055
22	20.732048235637954	23.91959032745848	30.507516169612565	24.840845267291005
23	21.235879385491188	24.59369480412214	30.813118670343215	23.357307140043456
24	21.067512103383866	24.014892562613568	32.52919425136918	22.38840108263339
25	20.399125760829513	25.237937913770537	32.019644967406634	22.343291357993316
26	20.020458213146625	25.489535814579973	31.13651092163615	23.353495050637253
27	21.994485177325693	24.987610709429838	32.22232105416979	20.79558305907468
28	19.294890529499217	24.36624013621866	33.1708959680801	23.167973366202016
29	21.21300684905397	23.679428694867656	31.851277685299312	23.256286770779063
30	21.340711844161785	23.6902296148519	32.03171658385961	22.9373419571267
31	23.18512776852993	24.361792698578093	29.054474757614653	23.398604775277327
32	23.718820285398426	24.100029226018783	29.14024676925423	23.040903719328565
33	22.915740117158215	24.147680343596324	29.304166613720977	23.63241292552448
34	21.737169142406955	23.198470081451646	30.62632628943924	24.43803448670216
35	22.558674409443817	22.34964484033699	30.876018145545576	24.21566260467362
36	24.52825393598231	22.984993074704246	30.660635094095074	21.82611789521837
37	22.6463524657865	24.19151937176767	31.614292793880328	21.54783536856551
38	22.223845889932274	25.570860388578982	29.480793422875077	22.72450029861367
39	24.53587811479472	23.081566006328067	30.727346658703635	21.655209220173578
40	22.15776967355808	22.35663367091503	33.2776344714538	22.20796218407309
41	20.68376176982604	23.261369556654003	30.630773727079813	25.424094946440146
42	22.380141555586615	23.38017967648068	30.162522078351145	24.07715668958156
43	24.19533146117387	20.90613365185458	30.27116662642795	24.6273682605436
44	22.12727295830845	22.788670470284764	29.747004333074955	25.337052238331832
45	22.150780842980037	23.16924406267075	30.023380815024716	24.656594279324498
46	22.885878750142954	23.89417639808379	31.16383089571394	22.056113956059317
47	21.395987140551735	22.67113104692682	32.9205687637394	23.012313048782037
48	21.813410930531024	21.997026570263163	31.98279476981333	24.20676772939248
49	22.70798124452012	21.758770982375438	32.52347611725987	23.009771655844567
50	21.338805799458683	21.790538394093804	32.19245968715453	24.678196119292984
51	21.519244698018987	23.107615283937125	29.20505228915969	26.168087728884203
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157.0
1	290.0
2	423.0
3	2340.5
4	4258.0
5	2699.0
6	1140.0
7	1130.0
8	1120.0
9	1124.0
10	1128.0
11	1146.0
12	1164.0
13	1168.0
14	1172.0
15	1089.5
16	1007.0
17	979.5
18	952.0
19	840.5
20	729.0
21	807.5
22	886.0
23	880.5
24	875.0
25	938.5
26	1203.5
27	1405.0
28	1537.5
29	1670.0
30	1869.0
31	2068.0
32	2219.5
33	2371.0
34	2597.5
35	2824.0
36	2974.0
37	3124.0
38	3470.5
39	3817.0
40	4602.0
41	5387.0
42	6281.0
43	7175.0
44	8659.0
45	10143.0
46	11580.0
47	13017.0
48	14069.0
49	15121.0
50	14504.0
51	13887.0
52	12274.5
53	10662.0
54	9405.5
55	8149.0
56	7452.5
57	6756.0
58	6528.5
59	6301.0
60	6052.0
61	5803.0
62	5350.0
63	4897.0
64	4507.5
65	4118.0
66	3785.0
67	3452.0
68	3001.0
69	2550.0
70	2256.0
71	1962.0
72	1897.5
73	1833.0
74	1516.0
75	948.5
76	698.0
77	560.5
78	423.0
79	337.5
80	252.0
81	238.0
82	224.0
83	129.5
84	35.0
85	33.0
86	31.0
87	23.5
88	16.0
89	12.0
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	157394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.326111541736026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.06423752134343	28.769203400383752
2	8.295343401356034	6.358565129547506
3	3.071796827080881	3.53190083484758
4	1.861644812094889	2.853984268777717
5	1.4041078858810072	2.690699772545332
6	1.1554465129386802	2.6570263161238676
7	0.9316512772905856	2.4994599540007876
8	0.862026092866734	2.643048654967788
9	0.8106360757919864	2.7961675794502967
>10	6.450276014123967	38.99640392899349
>50	0.05802098701987633	1.5318245930594558
>100	0.03149710723936144	2.395898191798925
>500	0.0016577424862821808	0.3240275995273009
>1k	0.0016577424862821808	1.9517897759762124
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3072	1.9517897759762124	No Hit
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	510	0.3240275995273009	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC	485	0.3081438936681195	No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT	379	0.24079698082519027	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	341	0.21665374791923453	TruSeq Adapter, Index 20 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	246	0.15629566565434513	No Hit
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	224	0.1423180044982655	TruSeq Adapter, Index 20 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	219	0.13914126332642923	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	202	0.12834034334218586	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	191	0.12135151276414602	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	189	0.12008081629541151	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC	172	0.10927989631116813	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	161	0.10229106573312832	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	158	0.10038502103002656	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25032720434069916	0.0
2	0.0	0.0	0.0	1.0457831937685045	0.0
3	0.0	0.0	0.0	1.437793054373102	0.0
4	0.0	0.0	0.0	2.0261255193971817	0.0
5	0.0	0.0	0.0	3.6144961053153235	0.0
6	0.0	0.0	0.0	4.508431071070054	0.0
7	0.0	0.0	0.0	5.434133448543147	0.0
8	0.0	0.0	0.0	6.838888394729151	0.0
9	0.0	0.0	0.0	7.488214290252487	0.0
10	0.0	0.0	0.0	8.821174885954992	0.0
11	0.0	0.0	0.0	10.474350991778595	0.0
12	0.0	0.0	0.0	11.80222880160616	0.0
13	0.0	0.0	0.0	12.250784655069443	0.0
14	0.0	0.0	0.0	12.444565866551457	0.0
15	0.0	0.0	0.0	12.826410155406178	0.0
16	0.0	0.0	0.0	13.614877314255944	0.0
17	0.0	0.0	0.0	14.529143423510426	0.0
18	0.0	0.0	0.0	15.640367485418759	0.0
19	0.0	0.0	0.0	16.167071171709214	0.0
20	0.0	0.0	0.0	16.69186881329657	0.0
21	0.0	0.0	0.0	17.281471974789383	0.0
22	0.0	0.0	0.0	17.827236108110856	0.0
23	0.0	0.0	0.0	18.394602081400816	0.0
24	0.0	0.0	0.0	18.824097487833082	0.0
25	0.0	0.0	0.0	19.230085009593758	0.0
26	0.0	0.0	0.0	19.589056762011257	0.0
27	0.0	0.0	0.0	19.953746648538065	0.0
28	0.0	0.0	0.0	20.34639185737703	0.0
29	0.0	0.0	0.0	20.730777539169218	0.0
30	0.0	0.0	0.0	21.129776230351855	0.0
31	0.0	0.0	0.0	21.512255867440945	0.0
32	0.0	0.0	0.0	21.85597926223363	0.0
33	0.0	0.0	0.0	22.256248649885002	0.0
34	0.0	0.0	0.0	22.617126447005603	0.0
35	0.0	0.0	0.0	22.978639592360572	0.0
36	0.0	0.0	0.0	23.343964827121745	0.0
37	0.0	0.0	0.0	23.721997026570264	0.0
38	0.0	0.0	0.0	24.073344600175357	0.0
39	0.0	0.0	0.0	24.42215078084298	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTAAG	20	7.014718E-4	45.000004	1
GGTACTT	30	2.1535325E-6	45.000004	7
AAATCAC	20	7.014718E-4	45.000004	19
CATATTA	40	6.7502697E-9	45.000004	28
ATTAGTC	30	2.1535325E-6	45.000004	31
CGTCTCG	20	7.014718E-4	45.000004	42
TCGATGG	20	7.014718E-4	45.000004	2
TGCGCCA	20	7.014718E-4	45.000004	19
CAAGGGT	20	7.014718E-4	45.000004	4
TAGATGA	20	7.014718E-4	45.000004	16
TATCGCG	20	7.014718E-4	45.000004	1
CGTCACG	20	7.014718E-4	45.000004	37
AAGGACC	20	7.014718E-4	45.000004	37
AGTACGG	20	7.014718E-4	45.000004	2
GTAACGG	20	7.014718E-4	45.000004	2
TTCAGTG	25	3.875424E-5	45.0	17
ACGTTGG	25	3.875424E-5	45.0	2
CTATCAC	25	3.875424E-5	45.0	19
TATGGGT	25	3.875424E-5	45.0	4
ATGTACG	25	3.875424E-5	45.0	1
>>END_MODULE