FastQCFastQC Report
Sat 18 Jun 2016
SRR3552018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473389
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.9142586752121407No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC7670.16202319868015522Illumina PCR Primer Index 6 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC6040.12759062842609356No Hit
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT5100.107733808770377Illumina PCR Primer Index 6 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG351.2099008E-745.0000041
TTGCGAG207.029076E-445.0000041
TGCGTAG207.029076E-445.0000041
CGTTTTT21300.041.302821
CCTCGAT1750.041.14285715
AGGGTAC800.039.3750046
CTCGATC1800.038.75000416
TTAGGCG356.2405034E-638.571431
AGTAACG356.2405034E-638.571431
CACAACG2350.038.2978712
ACAACGA2300.038.15217613
GGAACAC2800.037.767868
TCGATCA1850.037.702717
CGGGTAC900.037.5000046
CAGGGTA900.037.5000045
AACGGGC900.037.5000044
TACGAGG900.037.5000042
CGTGCGG601.546141E-1037.4999962
CGCATCG301.1389077E-437.49999621
TTCACGG301.1389077E-437.4999962