Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552018_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 473389 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 4328 | 0.9142586752121407 | No Hit |
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC | 767 | 0.16202319868015522 | Illumina PCR Primer Index 6 (95% over 23bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC | 604 | 0.12759062842609356 | No Hit |
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT | 510 | 0.107733808770377 | Illumina PCR Primer Index 6 (95% over 24bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTTACG | 35 | 1.2099008E-7 | 45.000004 | 1 |
TTGCGAG | 20 | 7.029076E-4 | 45.000004 | 1 |
TGCGTAG | 20 | 7.029076E-4 | 45.000004 | 1 |
CGTTTTT | 2130 | 0.0 | 41.30282 | 1 |
CCTCGAT | 175 | 0.0 | 41.142857 | 15 |
AGGGTAC | 80 | 0.0 | 39.375004 | 6 |
CTCGATC | 180 | 0.0 | 38.750004 | 16 |
TTAGGCG | 35 | 6.2405034E-6 | 38.57143 | 1 |
AGTAACG | 35 | 6.2405034E-6 | 38.57143 | 1 |
CACAACG | 235 | 0.0 | 38.29787 | 12 |
ACAACGA | 230 | 0.0 | 38.152176 | 13 |
GGAACAC | 280 | 0.0 | 37.76786 | 8 |
TCGATCA | 185 | 0.0 | 37.7027 | 17 |
CGGGTAC | 90 | 0.0 | 37.500004 | 6 |
CAGGGTA | 90 | 0.0 | 37.500004 | 5 |
AACGGGC | 90 | 0.0 | 37.500004 | 4 |
TACGAGG | 90 | 0.0 | 37.500004 | 2 |
CGTGCGG | 60 | 1.546141E-10 | 37.499996 | 2 |
CGCATCG | 30 | 1.1389077E-4 | 37.499996 | 21 |
TTCACGG | 30 | 1.1389077E-4 | 37.499996 | 2 |