##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	473389
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31309768499057	33.0	31.0	34.0	30.0	34.0
2	32.407590797420305	34.0	31.0	34.0	31.0	34.0
3	32.412736671109805	34.0	31.0	34.0	30.0	34.0
4	35.956976186603406	37.0	35.0	37.0	35.0	37.0
5	36.026789807114234	37.0	35.0	37.0	35.0	37.0
6	36.02578217913809	37.0	35.0	37.0	35.0	37.0
7	36.221428888292714	37.0	37.0	37.0	35.0	37.0
8	36.08686724871089	37.0	37.0	37.0	35.0	37.0
9	38.00765755013319	39.0	38.0	39.0	35.0	39.0
10	37.62164308845368	39.0	37.0	39.0	35.0	39.0
11	37.56786279360104	39.0	37.0	39.0	35.0	39.0
12	37.44921195887526	39.0	37.0	39.0	35.0	39.0
13	37.43712676044437	39.0	37.0	39.0	35.0	39.0
14	38.679318699843044	40.0	38.0	41.0	35.0	41.0
15	38.74984209603519	40.0	38.0	41.0	35.0	41.0
16	38.72463449721054	40.0	38.0	41.0	35.0	41.0
17	38.63460916920334	40.0	38.0	41.0	35.0	41.0
18	38.553094812089	40.0	38.0	41.0	35.0	41.0
19	38.43828859563699	40.0	37.0	41.0	34.0	41.0
20	38.3883148953609	40.0	37.0	41.0	34.0	41.0
21	38.25499536322137	40.0	36.0	41.0	34.0	41.0
22	38.300347071858454	40.0	37.0	41.0	34.0	41.0
23	38.26598632414357	40.0	36.0	41.0	34.0	41.0
24	38.19107964063381	40.0	36.0	41.0	34.0	41.0
25	38.02894237086202	40.0	36.0	41.0	34.0	41.0
26	38.06781526397952	40.0	36.0	41.0	34.0	41.0
27	38.072779468893444	40.0	36.0	41.0	34.0	41.0
28	37.995048469651806	40.0	36.0	41.0	34.0	41.0
29	37.93592162048548	40.0	36.0	41.0	34.0	41.0
30	37.744841979851664	40.0	35.0	41.0	33.0	41.0
31	37.680586156416815	40.0	35.0	41.0	33.0	41.0
32	37.536440432709675	40.0	35.0	41.0	33.0	41.0
33	37.40174993504285	40.0	35.0	41.0	33.0	41.0
34	37.20324088645913	40.0	35.0	41.0	33.0	41.0
35	37.078288680134094	40.0	35.0	41.0	32.0	41.0
36	36.99480765290279	40.0	35.0	41.0	32.0	41.0
37	36.97967422141199	40.0	35.0	41.0	32.0	41.0
38	36.87497174628054	39.0	35.0	41.0	32.0	41.0
39	36.82006341507724	39.0	35.0	41.0	32.0	41.0
40	36.71419065504268	39.0	35.0	41.0	31.0	41.0
41	36.63940226747981	39.0	35.0	41.0	31.0	41.0
42	36.53864580714803	39.0	35.0	41.0	31.0	41.0
43	36.38453153748819	39.0	35.0	41.0	31.0	41.0
44	36.37764291100976	39.0	35.0	41.0	31.0	41.0
45	36.29467731611846	39.0	35.0	41.0	31.0	41.0
46	36.195410117260856	38.0	35.0	41.0	31.0	41.0
47	36.12643935537159	38.0	35.0	41.0	31.0	41.0
48	36.080301823658765	38.0	35.0	41.0	31.0	41.0
49	36.021130613512355	38.0	35.0	41.0	31.0	41.0
50	35.892014812342495	38.0	35.0	40.0	31.0	41.0
51	35.06922002834878	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	5.0
11	13.0
12	14.0
13	11.0
14	10.0
15	19.0
16	33.0
17	46.0
18	112.0
19	194.0
20	326.0
21	511.0
22	759.0
23	1120.0
24	1884.0
25	2891.0
26	3992.0
27	5062.0
28	5329.0
29	5704.0
30	6128.0
31	7480.0
32	9282.0
33	13033.0
34	27023.0
35	39524.0
36	34234.0
37	50215.0
38	91167.0
39	167084.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65899714611028	18.887215376783153	24.876792658891524	13.576994818215042
2	34.092258164004654	22.455105631943287	25.057616463415922	18.395019740636137
3	32.37400953549829	22.920895922803446	28.305685176461644	16.39940936523662
4	27.449095775355996	25.60389024671042	28.029590886142262	18.91742309179132
5	25.066488659432306	29.199453303731183	26.617433020201148	19.116625016635368
6	25.88611057713635	32.664468333653716	25.826751360931492	15.622669728278435
7	79.39263480984982	5.642505423657922	10.509749909693719	4.455109856798532
8	81.37451440570017	4.745357412191665	9.663300161178228	4.216828020929933
9	75.8205196994438	6.335170441222758	11.925076417069262	5.919233442264185
10	39.771519828301884	24.928547135653766	19.051139760323963	16.24879327572039
11	31.841466531752953	25.13535379994043	24.611049263924595	18.412130404382022
12	28.697329257756305	22.12915804972232	28.60184752919903	20.571665163322344
13	25.137466227563376	23.01680013688531	30.873340952155626	20.972392683395686
14	22.147536170041974	25.025507563547105	30.786520176852438	22.040436089558483
15	22.02902898039456	23.502447247401186	33.186237956522014	21.28228581568224
16	25.004594530079917	22.966101873934544	31.312937140491226	20.71636645549432
17	23.600463889106	23.123266489081917	30.272355293426763	23.003914328385324
18	26.13347585178363	22.384128063812213	29.73051760814045	21.751878476263705
19	25.341315493177913	25.5267866384728	27.636256862749242	21.495641005600046
20	27.87496118414243	24.23545963256434	27.483950831134646	20.405628352158583
21	24.894114565399704	26.848743844914015	28.137747180437227	20.119394409249054
22	25.019592766202848	22.976452769286993	28.20259870846175	23.80135575604841
23	24.63724336644916	25.74732408230863	28.094442414166785	21.52099013707543
24	24.037102678769468	23.43696199108978	30.16060787217278	22.36532745796797
25	23.80093327052382	24.502259241342745	28.097611055601206	23.599196432532228
26	23.079961722811472	24.7504694870392	28.84857907555942	23.320989714589903
27	23.64651481128628	24.458109504023117	28.16309631191261	23.732279372777988
28	22.181968740296035	26.23022503691467	28.476791813920478	23.111014408868815
29	24.129627008654616	25.48601678534989	27.681674056642635	22.702682149352857
30	25.453062914431897	23.454072654835663	29.321551620337612	21.771312810394832
31	26.28937301035723	24.645059348654065	26.233393678349092	22.832173962639605
32	25.597975449366167	25.39201375612868	26.653133047028977	22.35687774747618
33	25.597764206603873	24.793563010547352	25.920120661865827	23.688552120982955
34	23.294161883778457	26.158613740496715	28.136902209388047	22.410322166336776
35	23.661301804646918	27.230459516380822	27.118289609602254	21.98994906937001
36	23.375067861737385	29.037430105051026	25.330753355063173	22.256748678148412
37	23.4361170200406	28.775700322567697	24.848908614268602	22.9392740431231
38	22.271958157033644	29.052428341173957	25.71120156995621	22.964411931836185
39	24.088223427244824	25.334766967546773	26.164951023365564	24.412058581842842
40	24.158356024326714	25.02804247669464	27.899254101806335	22.914347397172303
41	21.775115180116142	26.57645192431594	26.308596101726067	25.33983679384185
42	23.516811755237235	25.649729926128405	25.893926559341264	24.939531759293097
43	23.458086267319267	24.43635149950675	27.06780258941378	25.037759643760204
44	23.485759069179892	24.500569299244386	26.948450428717187	25.065221202858538
45	23.818466419794294	23.784245092302527	27.329743614659403	25.06754487324378
46	23.26120801286046	25.50164874975971	27.10878368529898	24.12835955208085
47	21.890876213853723	25.918853205292052	28.632477729731786	23.55779285112244
48	22.715779200615138	24.741597291022817	28.558542762928585	23.98408074543346
49	23.712633795884567	24.212222928711906	27.917843464888286	24.15729981051524
50	21.091533601329985	24.86348436486695	28.87688560570694	25.16809642809613
51	21.292847953796983	23.786146277163176	28.233862637281394	26.68714313175845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	270.0
1	573.5
2	877.0
3	4067.0
4	7257.0
5	4572.5
6	1888.0
7	1878.0
8	1868.0
9	1851.0
10	1834.0
11	1842.0
12	1850.0
13	1792.0
14	1734.0
15	1650.5
16	1567.0
17	1504.0
18	1441.0
19	1383.0
20	1325.0
21	1339.0
22	1353.0
23	1600.5
24	1848.0
25	2311.0
26	3263.0
27	3752.0
28	4225.5
29	4699.0
30	5315.5
31	5932.0
32	6580.0
33	7228.0
34	8459.0
35	9690.0
36	10360.0
37	11030.0
38	11875.0
39	12720.0
40	14884.0
41	17048.0
42	19031.5
43	21015.0
44	23417.5
45	25820.0
46	28643.0
47	31466.0
48	34952.0
49	38438.0
50	38735.5
51	39033.0
52	36910.5
53	34788.0
54	32931.0
55	31074.0
56	29904.5
57	28735.0
58	27825.5
59	26916.0
60	25632.0
61	24348.0
62	22761.0
63	21174.0
64	18307.0
65	15440.0
66	12928.0
67	10416.0
68	8743.5
69	7071.0
70	6203.5
71	5336.0
72	4913.5
73	4491.0
74	3810.0
75	2603.5
76	2078.0
77	1685.0
78	1292.0
79	974.5
80	657.0
81	550.0
82	443.0
83	293.5
84	144.0
85	93.0
86	42.0
87	33.0
88	24.0
89	23.0
90	22.0
91	13.0
92	4.0
93	2.5
94	1.0
95	4.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	473389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.65980848434939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.63975409466077	23.397406485975054
2	8.059181481993535	5.264226474850479
3	3.216562095928964	3.1515690609317226
4	2.0853410561114663	2.7242735806860554
5	1.6217992523249962	2.64838264904977
6	1.392645439223364	2.7290119998982623
7	1.2173099673309844	2.782997727938369
8	1.1175360915597898	2.9198811779752862
9	0.9954741470848859	2.9260795495422083
>10	8.553771312157734	46.91861357959661
>50	0.0655685885417675	1.4689126601335125
>100	0.03245969729790471	1.7521787273878966
>500	0.0019475818378742824	0.39881996445022183
>1k	6.491939459580941E-4	0.9176463615845615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4328	0.9142586752121407	No Hit
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	767	0.16202319868015522	Illumina PCR Primer Index 6 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC	604	0.12759062842609356	No Hit
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	510	0.107733808770377	Illumina PCR Primer Index 6 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1124276229485687E-4	0.0	0.0	0.12273204489331184	0.0
2	2.1124276229485687E-4	0.0	0.0	0.46410034876180056	0.0
3	2.1124276229485687E-4	0.0	0.0	0.6920312892779511	0.0
4	2.1124276229485687E-4	0.0	0.0	0.9926297400235324	0.0
5	2.1124276229485687E-4	0.0	0.0	1.7750729315636824	0.0
6	2.1124276229485687E-4	0.0	0.0	2.397182866522036	0.0
7	2.1124276229485687E-4	0.0	0.0	2.982959046365674	0.0
8	2.1124276229485687E-4	0.0	0.0	4.05184742357765	0.0
9	2.1124276229485687E-4	0.0	0.0	4.582277999700035	0.0
10	2.1124276229485687E-4	0.0	0.0	5.502662715018727	0.0
11	2.1124276229485687E-4	0.0	0.0	6.549581844952037	0.0
12	2.1124276229485687E-4	0.0	0.0	7.325898996385637	0.0
13	2.1124276229485687E-4	0.0	0.0	7.6588175897623305	0.0
14	2.1124276229485687E-4	0.0	0.0	7.788309403049078	0.0
15	2.1124276229485687E-4	0.0	0.0	8.024901296819317	0.0
16	2.1124276229485687E-4	0.0	0.0	8.519843088876167	0.0
17	2.1124276229485687E-4	0.0	0.0	9.091677246408345	0.0
18	2.1124276229485687E-4	0.0	0.0	9.724983047768326	0.0
19	2.1124276229485687E-4	0.0	0.0	10.066351351636815	0.0
20	2.1124276229485687E-4	0.0	0.0	10.429055174497083	0.0
21	2.1124276229485687E-4	0.0	0.0	10.875622373988412	0.0
22	2.1124276229485687E-4	0.0	0.0	11.307191337356803	0.0
23	2.1124276229485687E-4	0.0	0.0	11.760729548003862	0.0
24	2.1124276229485687E-4	0.0	0.0	12.142656462232964	0.0
25	2.1124276229485687E-4	0.0	0.0	12.474941327322773	0.0
26	2.1124276229485687E-4	0.0	0.0	12.797086539822429	0.0
27	2.1124276229485687E-4	0.0	0.0	13.104444758961446	0.0
28	4.2248552458971374E-4	0.0	0.0	13.418351503731603	0.0
29	4.2248552458971374E-4	0.0	0.0	13.741341687280439	0.0
30	4.2248552458971374E-4	0.0	0.0	14.122212387698067	0.0
31	4.2248552458971374E-4	0.0	0.0	14.458933350796068	0.0
32	4.2248552458971374E-4	0.0	0.0	14.813609948689132	0.0
33	4.2248552458971374E-4	0.0	0.0	15.167864061057609	0.0
34	6.337282868845706E-4	0.0	0.0	15.507753665590032	0.0
35	6.337282868845706E-4	0.0	0.0	15.874893586458494	0.0
36	6.337282868845706E-4	0.0	0.0	16.23485125340893	0.0
37	6.337282868845706E-4	0.0	0.0	16.605582301236403	0.0
38	6.337282868845706E-4	0.0	0.0	17.005042364735978	0.0
39	6.337282868845706E-4	0.0	0.0	17.40576988480932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	35	1.2099008E-7	45.000004	1
TTGCGAG	20	7.029076E-4	45.000004	1
TGCGTAG	20	7.029076E-4	45.000004	1
CGTTTTT	2130	0.0	41.30282	1
CCTCGAT	175	0.0	41.142857	15
AGGGTAC	80	0.0	39.375004	6
CTCGATC	180	0.0	38.750004	16
TTAGGCG	35	6.2405034E-6	38.57143	1
AGTAACG	35	6.2405034E-6	38.57143	1
CACAACG	235	0.0	38.29787	12
ACAACGA	230	0.0	38.152176	13
GGAACAC	280	0.0	37.76786	8
TCGATCA	185	0.0	37.7027	17
CGGGTAC	90	0.0	37.500004	6
CAGGGTA	90	0.0	37.500004	5
AACGGGC	90	0.0	37.500004	4
TACGAGG	90	0.0	37.500004	2
CGTGCGG	60	1.546141E-10	37.499996	2
CGCATCG	30	1.1389077E-4	37.499996	21
TTCACGG	30	1.1389077E-4	37.499996	2
>>END_MODULE