##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	615538
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.358629361631614	33.0	31.0	34.0	30.0	34.0
2	32.456483596463585	34.0	31.0	34.0	31.0	34.0
3	32.43664404147266	34.0	31.0	34.0	30.0	34.0
4	35.988611588561554	37.0	35.0	37.0	35.0	37.0
5	36.05186844678964	37.0	35.0	37.0	35.0	37.0
6	36.045337574609526	37.0	35.0	37.0	35.0	37.0
7	36.234370583132154	37.0	37.0	37.0	35.0	37.0
8	36.08548294337636	37.0	37.0	37.0	35.0	37.0
9	38.00885241853468	39.0	39.0	39.0	35.0	39.0
10	37.64264757009315	39.0	37.0	39.0	35.0	39.0
11	37.58461865879929	39.0	37.0	39.0	35.0	39.0
12	37.55190581247624	39.0	37.0	39.0	35.0	39.0
13	37.560522664725816	39.0	37.0	39.0	35.0	39.0
14	38.86206213101384	40.0	38.0	41.0	35.0	41.0
15	38.92118277019453	40.0	38.0	41.0	35.0	41.0
16	38.92141833647963	40.0	38.0	41.0	35.0	41.0
17	38.85093852857175	40.0	38.0	41.0	35.0	41.0
18	38.721932683278695	40.0	38.0	41.0	35.0	41.0
19	38.586933706773586	40.0	37.0	41.0	35.0	41.0
20	38.45502308549594	40.0	37.0	41.0	35.0	41.0
21	38.356146980365146	40.0	37.0	41.0	34.0	41.0
22	38.380736851339805	40.0	37.0	41.0	35.0	41.0
23	38.32755573173387	40.0	37.0	41.0	34.0	41.0
24	38.27123426985824	40.0	37.0	41.0	34.0	41.0
25	38.12590774249518	40.0	36.0	41.0	34.0	41.0
26	38.1504732445438	40.0	36.0	41.0	34.0	41.0
27	38.14352322683571	40.0	36.0	41.0	34.0	41.0
28	38.03936068934818	40.0	36.0	41.0	34.0	41.0
29	37.976605830996625	40.0	36.0	41.0	34.0	41.0
30	37.79901971933496	40.0	36.0	41.0	33.0	41.0
31	37.71207788958602	40.0	36.0	41.0	33.0	41.0
32	37.51039903304101	40.0	36.0	41.0	33.0	41.0
33	37.32005010251195	40.0	36.0	41.0	33.0	41.0
34	37.1284356124236	40.0	35.0	41.0	32.0	41.0
35	36.972690556878696	40.0	35.0	41.0	32.0	41.0
36	36.86982444625677	40.0	35.0	41.0	31.0	41.0
37	36.79813756421212	40.0	35.0	41.0	31.0	41.0
38	36.69797802897628	40.0	35.0	41.0	31.0	41.0
39	36.672463763406974	40.0	35.0	41.0	31.0	41.0
40	36.57819338529871	40.0	35.0	41.0	31.0	41.0
41	36.504556989170446	39.0	35.0	41.0	31.0	41.0
42	36.37885069646391	39.0	35.0	41.0	30.0	41.0
43	36.26442721651628	39.0	35.0	41.0	30.0	41.0
44	36.29511256819238	39.0	35.0	41.0	30.0	41.0
45	36.27358505892406	39.0	35.0	41.0	30.0	41.0
46	36.2115970094454	39.0	35.0	41.0	30.0	41.0
47	36.15404410450695	39.0	35.0	41.0	30.0	41.0
48	36.1180788188544	39.0	35.0	41.0	30.0	41.0
49	36.069623646306155	39.0	35.0	41.0	30.0	41.0
50	35.966547962920245	39.0	35.0	41.0	30.0	41.0
51	35.19923871475035	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	4.0
10	10.0
11	7.0
12	9.0
13	11.0
14	18.0
15	18.0
16	42.0
17	76.0
18	146.0
19	263.0
20	494.0
21	823.0
22	1256.0
23	1839.0
24	2894.0
25	4618.0
26	6765.0
27	8227.0
28	8332.0
29	7914.0
30	7928.0
31	9285.0
32	11382.0
33	15386.0
34	28872.0
35	42805.0
36	42344.0
37	61323.0
38	112848.0
39	239335.0
40	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46963794274277	19.217822457752405	24.176736448440224	16.1358031510646
2	34.662035487654705	24.752168022120486	24.188108613927977	16.397687876296832
3	30.60802095077802	23.79333201199601	29.902296852509515	15.69635018471646
4	27.0499952886743	24.160815416757373	30.766743889085646	18.022445405482685
5	25.985235680006756	28.224415064545163	27.13203733969308	18.658311915755
6	25.80198135614698	33.74820076096033	26.073613651797288	14.376204231095398
7	77.65613170917149	5.351903538043143	13.162956633059212	3.8290081197261587
8	78.80975666815047	5.8292095695147985	11.461030838063612	3.900002924271125
9	72.58300868508523	7.4778486462249285	13.418342978012731	6.520799690677098
10	36.67637091454955	29.19770997078978	20.4320448128304	13.69387430183027
11	28.173402779357243	23.537458288521588	30.146148572468313	18.142990359652856
12	25.102105800129316	21.55853903414574	32.836315548349575	20.503039617375368
13	23.816726180999385	22.27709743346471	34.37139542968915	19.534780955846756
14	20.976284161172828	25.74414577166641	32.49498812421004	20.78458194295072
15	20.41449918607787	23.936783756648655	35.24981398386452	20.398903073408952
16	22.856752954326133	23.150479742924077	33.107785384492914	20.884981918256877
17	22.440694156981372	23.299780029827566	31.477016853549255	22.78250895964181
18	22.4977174439271	23.175823426011068	32.151386266972956	22.175072863088875
19	22.938795005344918	24.757529185850427	30.369043016028257	21.9346327927764
20	24.085434205524272	24.896269604801002	30.99191276574314	20.026383423931584
21	23.44323177448021	25.344657844032376	30.987526359055007	20.22458402243241
22	22.609489584721008	22.62476077837599	30.784614434852113	23.981135202050886
23	21.908314352647604	24.804155064350212	30.695099246512807	22.59243133648938
24	21.513375291208668	24.697581627779275	31.394812343023503	22.394230737988558
25	21.504927396846334	26.451981843525502	29.914156396518166	22.128934363109995
26	20.571110150794915	25.9597295374128	30.69526170601977	22.773898605772512
27	22.699004773060313	24.800580955196917	30.206583509060366	22.2938307626824
28	20.3030519642979	25.829924391345454	31.648249173893404	22.218774470463238
29	21.461713167992876	24.356254203639743	30.66520669723072	23.516825931136665
30	22.19538030145986	25.892308842021126	29.884426306743045	22.02788454977597
31	22.8478176814429	26.480249797737915	27.634524594744757	23.037407926074426
32	22.282133678180713	27.266878730476428	28.02848889914189	22.422498692200968
33	23.325773550942426	26.267265384103013	26.88997267431093	23.516988390643633
34	21.46317530355559	26.366040764339488	28.05318274420101	24.117601187903915
35	22.031946037450165	25.63562282101186	28.55014637601578	23.782284765522192
36	23.014663595098924	27.87935107174537	27.1430845861669	21.962900746988815
37	22.165812671191706	27.642647570093153	28.232700499400526	21.958839259314615
38	22.089781621930733	27.40594406844094	27.880488288294142	22.623786021334183
39	23.663039487407765	25.49655748304735	27.848158846407532	22.992244183137352
40	23.222124385496915	24.359340934272133	29.76388135257287	22.65465332765808
41	21.36001351663098	25.17878668741816	29.122978597584552	24.33822119836631
42	21.754952578069915	25.81205384557899	29.48087689143481	22.952116684916284
43	23.073636396128265	25.059866328317664	28.732750861847684	23.133746413706383
44	22.295130438738145	25.96525316064971	28.22409014553123	23.51552625508092
45	21.936744766366985	24.972300654061975	28.70529520517011	24.38565937440093
46	22.90841507754192	25.896857708216224	28.675727574901956	22.518999639339896
47	21.24905367337191	25.756817613209908	30.391624887496793	22.60250382592139
48	21.64951635804776	25.411428701396176	29.356107990083473	23.582946950472593
49	22.0368198226592	24.68425994820791	30.064593899970433	23.214326329162457
50	21.09747895337087	24.30199272831247	30.270755014312684	24.329773304003975
51	21.30380252722009	24.505879409557167	28.587349603111424	25.60296846011132
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	325.0
1	673.5
2	1022.0
3	6918.5
4	12815.0
5	8280.0
6	3745.0
7	3652.5
8	3560.0
9	3525.5
10	3491.0
11	3428.0
12	3365.0
13	3217.5
14	3070.0
15	2900.0
16	2730.0
17	2691.5
18	2653.0
19	2453.0
20	2253.0
21	2334.5
22	2416.0
23	2646.5
24	2877.0
25	3465.0
26	4567.0
27	5081.0
28	6471.5
29	7862.0
30	8424.0
31	8986.0
32	10891.5
33	12797.0
34	13836.5
35	14876.0
36	16495.0
37	18114.0
38	18853.0
39	19592.0
40	23446.0
41	27300.0
42	29304.5
43	31309.0
44	34207.0
45	37105.0
46	44614.5
47	52124.0
48	52272.0
49	52420.0
50	50826.0
51	49232.0
52	44678.0
53	40124.0
54	36058.5
55	31993.0
56	30233.0
57	28473.0
58	27241.0
59	26009.0
60	25300.0
61	24591.0
62	22593.5
63	20596.0
64	18562.0
65	16528.0
66	14170.5
67	11813.0
68	10293.5
69	8774.0
70	7780.0
71	6786.0
72	6063.0
73	5340.0
74	4429.5
75	3087.5
76	2656.0
77	2141.5
78	1627.0
79	1214.0
80	801.0
81	582.0
82	363.0
83	290.0
84	217.0
85	159.5
86	102.0
87	67.0
88	32.0
89	24.0
90	16.0
91	9.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	615538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.967148347798425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.74044676592638	22.525642058855762
2	7.9538088316039115	4.926135560366152
3	3.0120661235446122	2.7982529544355246
4	1.8514044756569938	2.293308681977923
5	1.334233943300441	2.065871022642641
6	1.1156956472043726	2.0729947570782468
7	0.9757255384663728	2.1150806247496563
8	0.9082273135037282	2.2500167960633903
9	0.8698596256700084	2.42433648628952
>10	9.119204855530496	49.69973790079255
>50	0.07814326628195839	1.650256046381472
>100	0.036431657928740424	2.247539840835694
>500	0.00158398512733654	0.25245173244946245
>1k	0.002639975212227567	1.3335468457628343
>5k	5.279950424455133E-4	1.3448286913191898
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8225	1.336229444810881	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCG	2193	0.35627369878057896	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTC	1911	0.31046011781563443	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGC	1870	0.30379927802995105	No Hit
GCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCCGTCTTCTGC	1179	0.19153975871514026	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAAGCTGTCGTATGCC	1003	0.1629468854887919	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08561616017207711	0.0
2	0.0	0.0	0.0	0.43896558782723405	0.0
3	0.0	0.0	0.0	0.6417150525231586	0.0
4	0.0	0.0	0.0	0.9999382653873522	0.0
5	0.0	0.0	0.0	2.0755826610217403	0.0
6	0.0	0.0	0.0	2.7403669635343393	0.0
7	0.0	0.0	0.0	3.376883311834525	0.0
8	0.0	0.0	0.0	4.336369159986873	0.0
9	0.0	0.0	0.0	4.804902378082263	0.0
10	0.0	0.0	0.0	5.987770048315458	0.0
11	0.0	0.0	0.0	6.98689601616797	0.0
12	0.0	0.0	0.0	8.03475983611085	0.0
13	0.0	0.0	0.0	8.368289203915923	0.0
14	0.0	0.0	0.0	8.513690462652184	0.0
15	0.0	0.0	0.0	8.787434731893075	0.0
16	0.0	0.0	0.0	9.2340359165478	0.0
17	0.0	0.0	0.0	9.759105043068015	0.0
18	0.0	0.0	0.0	10.362804570960687	0.0
19	0.0	0.0	0.0	10.779188287319386	0.0
20	0.0	0.0	0.0	11.139685933281129	0.0
21	0.0	0.0	0.0	11.537874184859424	0.0
22	0.0	0.0	0.0	11.952145927627539	0.0
23	0.0	0.0	0.0	12.380876566515797	0.0
24	0.0	0.0	0.0	12.733251237129146	0.0
25	0.0	0.0	0.0	13.049884816209559	0.0
26	0.0	0.0	0.0	13.347998011495635	0.0
27	0.0	0.0	0.0	13.650984991990747	0.0
28	0.0	0.0	0.0	13.948448349248949	0.0
29	0.0	0.0	0.0	14.275154417761373	0.0
30	0.0	0.0	0.0	14.632727792597695	0.0
31	0.0	0.0	0.0	14.973405378709357	0.0
32	0.0	0.0	0.0	15.28776452469222	0.0
33	0.0	0.0	0.0	15.621456352004262	0.0
34	0.0	0.0	0.0	15.95401096276753	0.0
35	0.0	0.0	0.0	16.29923741507429	0.0
36	0.0	0.0	0.0	16.631304647316657	0.0
37	0.0	0.0	0.0	16.953949228154883	0.0
38	0.0	0.0	0.0	17.303724546656746	0.0
39	0.0	0.0	0.0	17.633679805308528	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATTCG	20	7.0307293E-4	45.000004	25
CATTCGC	20	7.0307293E-4	45.000004	26
TACGCGG	35	1.2107012E-7	45.000004	2
ACGTCGG	40	6.8066583E-9	45.000004	2
ATAACGG	45	3.8380676E-10	45.000004	2
CGTCATA	20	7.0307293E-4	45.000004	38
TATAACG	20	7.0307293E-4	45.000004	1
GTACGAG	20	7.0307293E-4	45.000004	1
TTACGCG	25	3.8886872E-5	45.0	1
CGTTTTT	3545	0.0	43.222847	1
CGAATAT	85	0.0	39.705883	14
TCGACGG	70	0.0	38.57143	2
CTAGCGG	100	0.0	38.25	2
ACGAAGG	95	0.0	37.894733	2
CGGCGAA	60	1.546141E-10	37.499996	31
CCTCGTC	60	1.546141E-10	37.499996	36
CCGTCGA	30	1.1393059E-4	37.499996	41
GCGATAT	30	1.1393059E-4	37.499996	9
CATCGTA	30	1.1393059E-4	37.499996	35
CTCGAGG	30	1.1393059E-4	37.499996	2
>>END_MODULE