FastQCFastQC Report
Sat 18 Jun 2016
SRR3552012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211462
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46272.1880999895962394No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG31101.4707134142304528No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC25821.2210231625540287No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC23681.1198229469124477No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC14040.6639490783214006No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCT6580.3111670181876649No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT4600.21753317380900586No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTA4510.21327708997361228No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCC4230.20003594026349888No Hit
GAACTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCT4080.19294246720450955No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTC3990.18868638336911597No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT3760.17780972467866565No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC3310.1565293055016977No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC2640.12484512583821206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTT207.020218E-445.0000049
ACCGGGC207.020218E-445.0000044
TAGCGAT207.020218E-445.00000436
TACGGGA207.020218E-445.0000044
AGTATGA351.2056444E-745.0000043
GTAATGC207.020218E-445.0000049
TAGTTCC302.1570759E-645.00000417
GTTCCCT302.1570759E-645.00000419
TTAACAG207.020218E-445.00000421
CCAAGCG302.1570759E-645.00000420
GGTGATC207.020218E-445.0000049
GGGTTAC207.020218E-445.0000047
GTAAGTG207.020218E-445.0000041
TAGTGCG207.020218E-445.0000041
ATTGAGG351.2056444E-745.0000042
GACCTAG207.020218E-445.0000041
CTAGGAA207.020218E-445.0000044
TAGTCAG207.020218E-445.00000433
GTAGTGC207.020218E-445.00000412
GATAGCG207.020218E-445.0000049