FastQCFastQC Report
Sat 18 Jun 2016
SRR3552011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422668
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50331.1907691142930148No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG9980.23611912896173828No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC8790.20796464364465725No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC7900.18690792773524373No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC7690.1819394891498765No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC6660.15757048085021816No Hit
CCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC5770.1365137649408046No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4850.11474727209062432No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT4600.10883246425090141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG600.045.0000042
CTATACG207.028218E-445.0000041
CGTTCAT207.028218E-445.00000417
TAGCCGG302.1622327E-645.0000042
GTATGCG207.028218E-445.0000041
TCCCGAA207.028218E-445.00000428
TATAGCG207.028218E-445.0000041
CAGTACG207.028218E-445.0000041
TCGACAG207.028218E-445.0000041
TACGAAT351.2094824E-745.012
GCTACGA351.2094824E-745.010
CGTTTTT21300.042.0422551
CGTAAGG501.0786607E-940.5000042
TATACGG451.9233994E-840.02
TACGGGT356.238919E-638.5714264
GCGATTA356.238919E-638.5714269
GGATTAC356.238919E-638.5714268
TTAGTCA659.094947E-1238.0769232
TTCACGG301.13870075E-437.5000042
TAAGTAG301.13870075E-437.5000041