##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	422668
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.364517777546446	33.0	31.0	34.0	31.0	34.0
2	32.46418465556891	34.0	31.0	34.0	31.0	34.0
3	32.442179204482	34.0	31.0	34.0	31.0	34.0
4	35.989388834735536	37.0	35.0	37.0	35.0	37.0
5	36.052743524468376	37.0	35.0	37.0	35.0	37.0
6	36.032872136050045	37.0	35.0	37.0	35.0	37.0
7	36.19885347364835	37.0	37.0	37.0	35.0	37.0
8	36.04985709824259	37.0	37.0	37.0	35.0	37.0
9	37.94807508493664	39.0	38.0	39.0	35.0	39.0
10	37.65767694739133	39.0	37.0	39.0	35.0	39.0
11	37.60123075321529	39.0	37.0	39.0	35.0	39.0
12	37.507261018104046	39.0	37.0	39.0	35.0	39.0
13	37.500780754634846	39.0	37.0	39.0	35.0	39.0
14	38.77415370929429	40.0	38.0	41.0	35.0	41.0
15	38.82045955690992	40.0	38.0	41.0	35.0	41.0
16	38.82566931965514	40.0	38.0	41.0	35.0	41.0
17	38.77253305194621	40.0	38.0	41.0	35.0	41.0
18	38.665941116904996	40.0	38.0	41.0	35.0	41.0
19	38.55514257052817	40.0	37.0	41.0	35.0	41.0
20	38.44768943946549	40.0	37.0	41.0	35.0	41.0
21	38.34801309775048	40.0	37.0	41.0	34.0	41.0
22	38.35671496304428	40.0	37.0	41.0	34.0	41.0
23	38.33114643171473	40.0	37.0	41.0	34.0	41.0
24	38.253555982473245	40.0	37.0	41.0	34.0	41.0
25	38.08133807148873	40.0	36.0	41.0	34.0	41.0
26	38.072316806571585	40.0	36.0	41.0	34.0	41.0
27	38.08748710571891	40.0	36.0	41.0	34.0	41.0
28	37.99776420263658	40.0	36.0	41.0	34.0	41.0
29	37.91895293705698	40.0	36.0	41.0	34.0	41.0
30	37.749396689600346	40.0	35.0	41.0	33.0	41.0
31	37.68038271172646	40.0	35.0	41.0	33.0	41.0
32	37.521745199541954	40.0	35.0	41.0	33.0	41.0
33	37.35428279406059	40.0	35.0	41.0	33.0	41.0
34	37.195654745568625	40.0	35.0	41.0	33.0	41.0
35	37.079083346740234	40.0	35.0	41.0	32.0	41.0
36	36.95230535550361	40.0	35.0	41.0	32.0	41.0
37	36.9359426310958	39.0	35.0	41.0	32.0	41.0
38	36.79471831319144	39.0	35.0	41.0	32.0	41.0
39	36.73523190778578	39.0	35.0	41.0	31.0	41.0
40	36.63619199939432	39.0	35.0	41.0	31.0	41.0
41	36.56279633187277	39.0	35.0	41.0	31.0	41.0
42	36.47708366850578	39.0	35.0	41.0	31.0	41.0
43	36.3575075472948	39.0	35.0	41.0	31.0	41.0
44	36.348344326989505	39.0	35.0	41.0	31.0	41.0
45	36.28520020441576	39.0	35.0	41.0	31.0	41.0
46	36.18994577304173	38.0	35.0	41.0	31.0	41.0
47	36.130577663792856	38.0	35.0	41.0	31.0	41.0
48	36.08535777489661	38.0	35.0	41.0	31.0	41.0
49	36.02596600641638	38.0	35.0	41.0	31.0	41.0
50	35.93402623335573	38.0	35.0	40.0	31.0	41.0
51	35.0933143744026	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	2.0
10	9.0
11	6.0
12	6.0
13	11.0
14	17.0
15	23.0
16	30.0
17	66.0
18	90.0
19	186.0
20	302.0
21	506.0
22	709.0
23	1148.0
24	1543.0
25	2454.0
26	3670.0
27	4468.0
28	4843.0
29	5081.0
30	5464.0
31	6581.0
32	8205.0
33	11593.0
34	23022.0
35	33356.0
36	32363.0
37	45892.0
38	80548.0
39	150302.0
40	168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.8363065100741	19.889606026479413	24.151816555783736	15.122270907662752
2	35.10840659808644	23.972716174396925	23.58375841085675	17.335118816659882
3	31.37852877435718	23.785808246661684	27.684612982293434	17.15104999668771
4	28.54273330368043	24.256153766076448	28.184532540906808	19.01658038933631
5	25.989192463115256	27.855905817331806	25.667663508947918	20.487238210605014
6	26.360169210822683	32.86858716534017	25.432963933867715	15.338279689969433
7	77.03516708149185	6.004476326573102	12.044441500184542	4.9159150917505
8	77.64770458137356	6.101952359771736	11.275753073334153	4.97458998552055
9	71.53061031353214	7.6308118901833115	13.202797467515875	7.635780328768679
10	39.57006444774622	24.06593354595096	19.678565682758098	16.68543632354472
11	30.665912725827365	23.437307768745207	25.762537026697075	20.134242478730354
12	27.637531111889235	20.441339301768764	30.5734051312141	21.3477244551279
13	25.154967965400736	21.62051539269592	32.004788628427036	21.2197280134763
14	21.794647335497363	24.855442096397173	30.693120841890085	22.656789726215372
15	20.61026621367125	23.089517067769503	33.63159737666443	22.66861934189482
16	22.815306576319948	22.88084264718408	31.491856492566267	22.811994283929703
17	23.068696944173677	22.639045302696207	29.730663310210375	24.561594442919738
18	23.913568095999697	22.73628474358125	29.534291689931578	23.815855470487474
19	24.919795205693358	24.3425099605364	28.036662344913736	22.7010324888565
20	26.405121750404575	23.99400948261993	28.199674448976502	21.401194317998996
21	25.624603707874737	24.55284052731695	28.061504537840577	21.761051226967737
22	24.2464534812193	23.139911230563943	27.881694379513	24.73194090870376
23	23.727843129832397	24.833202418919814	28.071914599638486	23.3670398516093
24	23.967984328125148	23.236677486821808	29.318756092252073	23.476582092800967
25	23.98383601313561	24.556152819707194	27.431222614439704	24.0287885527175
26	22.631001164034185	25.543452544313737	27.705906290516435	24.119640001135643
27	24.677997861205487	24.575789981735074	26.91805388626534	23.8281582707941
28	22.833760776779886	25.612300907568113	28.04470648357576	23.50923183207624
29	23.84400995580456	25.0106466541115	27.374440459178363	23.770902930905578
30	24.96261841445295	24.69668865397901	27.58453443364532	22.75615849792272
31	25.49400475077366	26.135643105226798	25.112144756641147	23.258207387358397
32	26.49171453717812	25.758514957366064	25.666007362752797	22.08376314270302
33	26.039350033596108	24.803864972034788	25.985643578411427	23.171141415957678
34	24.942034883170717	25.712379456216226	26.69707666537329	22.648508995239762
35	25.72066018719184	24.522793303491156	26.159775521212868	23.596770988104137
36	24.71159396973511	27.310560534509353	26.29818202466238	21.679663471093154
37	25.341402708508802	26.660168264453425	26.344554118125814	21.65387490891196
38	24.069482430654794	25.752600149526344	26.775861905798404	23.40205551402046
39	25.74124371847407	23.448427607483886	27.475228784767243	23.335099889274797
40	25.153548411519207	22.989911703748568	29.233582859360062	22.62295702537216
41	22.706474112069046	24.912934028599278	27.870101356147142	24.51049050318453
42	22.76065375188091	25.901653307087358	27.57152185639793	23.766171084633804
43	23.91498764988123	23.96751114349797	28.365525660802334	23.751975545818468
44	24.483992164062574	24.285964397588653	27.46316257677421	23.76688086157457
45	24.505758656912754	23.653079958738303	26.843290715171246	24.9978706691777
46	24.451105832473715	24.875079258425053	27.1111132141539	23.562701694947336
47	23.52177122469645	24.512856426320422	29.12333084122763	22.842041507755496
48	23.186756508654547	23.774925000236593	28.661502645102065	24.376815846006792
49	23.91238513443175	23.27051018766502	29.504007873792197	23.313096804111026
50	22.40481891224318	23.115305629950694	29.303377591868795	25.176497865937332
51	22.1930687915811	23.076031305894933	27.494865946795123	27.236033955728843
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	235.0
1	579.5
2	924.0
3	4196.0
4	7468.0
5	4723.5
6	1979.0
7	1867.0
8	1755.0
9	1763.5
10	1772.0
11	1704.0
12	1636.0
13	1585.0
14	1534.0
15	1459.0
16	1384.0
17	1357.0
18	1330.0
19	1366.5
20	1403.0
21	1385.5
22	1368.0
23	1453.0
24	1538.0
25	1935.0
26	2670.0
27	3008.0
28	3527.5
29	4047.0
30	4978.5
31	5910.0
32	6313.5
33	6717.0
34	7060.5
35	7404.0
36	8416.0
37	9428.0
38	10094.5
39	10761.0
40	12225.0
41	13689.0
42	14810.5
43	15932.0
44	18463.5
45	20995.0
46	25796.5
47	30598.0
48	32721.0
49	34844.0
50	34810.0
51	34776.0
52	31834.0
53	28892.0
54	27289.5
55	25687.0
56	25066.5
57	24446.0
58	23337.5
59	22229.0
60	21938.0
61	21647.0
62	20349.0
63	19051.0
64	17128.5
65	15206.0
66	13044.5
67	10883.0
68	9596.0
69	8309.0
70	7483.5
71	6658.0
72	6140.0
73	5622.0
74	4685.5
75	3200.0
76	2651.0
77	2016.0
78	1381.0
79	1087.5
80	794.0
81	614.0
82	434.0
83	292.0
84	150.0
85	122.5
86	95.0
87	51.0
88	7.0
89	8.0
90	9.0
91	4.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	422668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.251737857707035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.4571475429456	24.83736895385706
2	7.978163581995698	5.624882623568347
3	3.6270735297159726	3.835819357805269
4	2.4545288753377625	3.461056339103171
5	2.0302396527289557	3.5784738013161648
6	1.7713615250465795	3.74661432793021
7	1.586123916281211	3.9139537154609445
8	1.4586532788100057	4.113605040791614
9	1.2748866364438376	4.044777255559081
>10	7.266140678758831	36.86032697992104
>50	0.05996894079055881	1.4299254460366142
>100	0.030995182880513547	2.2463131134830996
>500	0.004042849940936549	1.1113988641204846
>1k	0.0	0.0
>5k	6.738083234894249E-4	1.1954841810468904
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5033	1.1907691142930148	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCG	998	0.23611912896173828	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTC	879	0.20796464364465725	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCC	790	0.18690792773524373	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGC	769	0.1819394891498765	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	666	0.15757048085021816	No Hit
CCTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGC	577	0.1365137649408046	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	485	0.11474727209062432	No Hit
CTGTCTCTTATACACATCTGACGCAGAGTGCTTCGTATGCCGTCTTCTGCT	460	0.10883246425090141	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11332771820909082	0.0
2	0.0	0.0	0.0	0.5320961132614723	0.0
3	0.0	0.0	0.0	0.8164800741953495	0.0
4	0.0	0.0	0.0	1.2118258302024283	0.0
5	0.0	0.0	0.0	2.3403711660215585	0.0
6	0.0	0.0	0.0	3.2001476336036796	0.0
7	0.0	0.0	0.0	3.920571228481929	0.0
8	0.0	0.0	0.0	4.940047507736569	0.0
9	0.0	0.0	0.0	5.429083819924858	0.0
10	0.0	0.0	0.0	6.549111832454787	0.0
11	0.0	0.0	0.0	7.790984886483008	0.0
12	0.0	0.0	0.0	8.835066766350895	0.0
13	0.0	0.0	0.0	9.20935580644856	0.0
14	0.0	0.0	0.0	9.379229087605403	0.0
15	0.0	0.0	0.0	9.68750887221176	2.3659231358891613E-4
16	0.0	0.0	0.0	10.233800524288567	2.3659231358891613E-4
17	0.0	0.0	0.0	10.822678792811379	2.3659231358891613E-4
18	0.0	0.0	0.0	11.45106797770354	2.3659231358891613E-4
19	0.0	0.0	0.0	11.863448380289022	2.3659231358891613E-4
20	0.0	0.0	0.0	12.24578155904871	2.3659231358891613E-4
21	0.0	0.0	0.0	12.679218677543604	2.3659231358891613E-4
22	0.0	0.0	0.0	13.161157220324226	2.3659231358891613E-4
23	0.0	0.0	0.0	13.623931785704146	2.3659231358891613E-4
24	0.0	0.0	0.0	13.984498471613653	2.3659231358891613E-4
25	0.0	0.0	0.0	14.285917079125934	2.3659231358891613E-4
26	0.0	0.0	0.0	14.595379825300236	2.3659231358891613E-4
27	0.0	0.0	0.0	14.933943426045975	2.3659231358891613E-4
28	0.0	0.0	0.0	15.271797249850946	2.3659231358891613E-4
29	0.0	0.0	0.0	15.587647988492149	2.3659231358891613E-4
30	0.0	0.0	0.0	15.972583682701316	2.3659231358891613E-4
31	0.0	0.0	0.0	16.328418522339046	2.3659231358891613E-4
32	0.0	0.0	0.0	16.70767600102208	2.3659231358891613E-4
33	0.0	0.0	0.0	17.048605524903707	2.3659231358891613E-4
34	0.0	0.0	0.0	17.381254317809724	2.3659231358891613E-4
35	0.0	0.0	0.0	17.708698079816784	2.3659231358891613E-4
36	0.0	0.0	0.0	18.053176488402244	2.3659231358891613E-4
37	0.0	0.0	0.0	18.40593562796332	2.3659231358891613E-4
38	0.0	0.0	0.0	18.749467667294425	2.3659231358891613E-4
39	0.0	0.0	0.0	19.10814161469522	2.3659231358891613E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	60	0.0	45.000004	2
CTATACG	20	7.028218E-4	45.000004	1
CGTTCAT	20	7.028218E-4	45.000004	17
TAGCCGG	30	2.1622327E-6	45.000004	2
GTATGCG	20	7.028218E-4	45.000004	1
TCCCGAA	20	7.028218E-4	45.000004	28
TATAGCG	20	7.028218E-4	45.000004	1
CAGTACG	20	7.028218E-4	45.000004	1
TCGACAG	20	7.028218E-4	45.000004	1
TACGAAT	35	1.2094824E-7	45.0	12
GCTACGA	35	1.2094824E-7	45.0	10
CGTTTTT	2130	0.0	42.042255	1
CGTAAGG	50	1.0786607E-9	40.500004	2
TATACGG	45	1.9233994E-8	40.0	2
TACGGGT	35	6.238919E-6	38.571426	4
GCGATTA	35	6.238919E-6	38.571426	9
GGATTAC	35	6.238919E-6	38.571426	8
TTAGTCA	65	9.094947E-12	38.07692	32
TTCACGG	30	1.13870075E-4	37.500004	2
TAAGTAG	30	1.13870075E-4	37.500004	1
>>END_MODULE