FastQCFastQC Report
Sat 18 Jun 2016
SRR3552009_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552009_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546820
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179893.289747997512893No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC20980.38367287224315133No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT18290.33447935335210854No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC16560.30284188581251603No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC15640.28601733660070955No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC13450.24596759445521377No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG12590.23024029845287297No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT11670.21341574924106654No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT11650.21304999817124468No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC10540.19275081379613035No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC10150.1856186679346037No Hit
CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG8770.16038184411689405No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG8490.15526132913938773No Hit
CGTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT8430.1541640759299221No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC7180.13130463406605464No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC6250.11429720931933726No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT6000.10972532094656376No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT5890.10771369006254343No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC5710.1044219304341465No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCC207.030038E-445.00000423
TCGACGT207.030038E-445.00000426
CGTTTAT207.030038E-445.00000439
GTTAGCG351.2103737E-745.01
ACTACGG253.888114E-545.02
ATAACGC253.888114E-545.011
TCGACGG351.2103737E-745.02
CGTTTTT73150.044.0772361
GCTACGA850.042.3529410
ACACGAC650.041.53846426
GACACGA650.041.53846425
CACGACC650.041.53846427
AGACACG650.041.53846424
GCGTTAG501.0786607E-940.51
CTACGAA900.040.011
ATAACGG451.9250365E-840.02
TTATAGG800.039.3750042
ATTGCGG800.039.3750042
AACACGT750.039.041
GTTTTTT84500.038.7426032
Produced by FastQC (version 0.11.5)