##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	546820
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.247710398302914	33.0	31.0	34.0	30.0	34.0
2	32.37797812808603	34.0	31.0	34.0	30.0	34.0
3	32.305762408105046	34.0	31.0	34.0	30.0	34.0
4	35.91728905307048	37.0	35.0	37.0	35.0	37.0
5	35.995188544676495	37.0	35.0	37.0	35.0	37.0
6	35.985796057203466	37.0	35.0	37.0	35.0	37.0
7	36.17527157016934	37.0	36.0	37.0	35.0	37.0
8	36.00795691452397	37.0	36.0	37.0	35.0	37.0
9	37.92015288394719	39.0	38.0	39.0	35.0	39.0
10	37.615743754800484	39.0	37.0	39.0	35.0	39.0
11	37.598074320617386	39.0	37.0	39.0	35.0	39.0
12	37.59965619399437	39.0	37.0	39.0	35.0	39.0
13	37.61890933030979	39.0	37.0	39.0	35.0	39.0
14	38.93607768552723	40.0	38.0	41.0	35.0	41.0
15	38.98897992026627	40.0	38.0	41.0	35.0	41.0
16	38.96266413079258	40.0	38.0	41.0	35.0	41.0
17	38.91092132694488	40.0	38.0	41.0	35.0	41.0
18	38.66600343806006	40.0	38.0	41.0	35.0	41.0
19	38.40773197761604	40.0	37.0	41.0	35.0	41.0
20	38.152227424015216	40.0	35.0	41.0	34.0	41.0
21	38.06034709776526	40.0	35.0	41.0	34.0	41.0
22	38.09995610987162	40.0	35.0	41.0	34.0	41.0
23	38.06163819904173	40.0	35.0	41.0	34.0	41.0
24	37.981906294575914	40.0	35.0	41.0	34.0	41.0
25	37.81208075783621	40.0	35.0	41.0	34.0	41.0
26	37.83025127098497	40.0	35.0	41.0	34.0	41.0
27	37.84004425587945	40.0	35.0	41.0	34.0	41.0
28	37.746128524925936	40.0	35.0	41.0	34.0	41.0
29	37.65791668190629	40.0	35.0	41.0	34.0	41.0
30	37.432021506162904	40.0	35.0	41.0	33.0	41.0
31	37.26243553637394	40.0	35.0	41.0	33.0	41.0
32	36.93287919242164	40.0	35.0	41.0	32.0	41.0
33	36.52165977835485	40.0	35.0	41.0	31.0	41.0
34	36.18687502285944	40.0	35.0	41.0	29.0	41.0
35	35.94593101934823	40.0	35.0	41.0	26.0	41.0
36	35.78447935335211	39.0	35.0	41.0	25.0	41.0
37	35.72906258000805	39.0	35.0	41.0	25.0	41.0
38	35.61777184448265	39.0	35.0	41.0	24.0	41.0
39	35.54634248930178	39.0	35.0	41.0	23.0	41.0
40	35.45891335357156	39.0	35.0	41.0	23.0	41.0
41	35.35659449178889	39.0	35.0	41.0	23.0	41.0
42	35.208271460444024	39.0	35.0	41.0	23.0	41.0
43	35.09955744120551	39.0	35.0	41.0	22.0	41.0
44	35.13061519329944	39.0	35.0	41.0	22.0	41.0
45	35.11864781829487	39.0	35.0	41.0	23.0	41.0
46	35.0472148056033	39.0	35.0	41.0	23.0	41.0
47	34.99807432061739	38.0	35.0	41.0	23.0	41.0
48	34.945963936944516	38.0	35.0	41.0	22.0	41.0
49	34.9078087853407	38.0	35.0	41.0	22.0	41.0
50	34.815762042353974	38.0	35.0	41.0	22.0	41.0
51	34.037650415127466	37.0	34.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	3.0
10	9.0
11	17.0
12	9.0
13	8.0
14	18.0
15	24.0
16	45.0
17	84.0
18	161.0
19	291.0
20	525.0
21	856.0
22	1314.0
23	2029.0
24	3497.0
25	6524.0
26	10257.0
27	11910.0
28	11198.0
29	9649.0
30	9001.0
31	9505.0
32	11132.0
33	14304.0
34	24792.0
35	36619.0
36	41183.0
37	52754.0
38	96934.0
39	191932.0
40	231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.21999926849786	18.399290442924546	22.04765736439779	24.333052924179803
2	39.191324384623826	22.865842507589335	22.3788449581215	15.563988149665336
3	27.527522768004097	22.1798763761384	35.221462272777146	15.071138583080357
4	24.412603781866064	22.484912768369846	36.16747741487144	16.935006034892652
5	22.221389122563185	25.652865659632056	33.906221425697666	18.219523792107093
6	22.097216634358656	30.01298416297868	33.57485095643904	14.314948246223619
7	68.7846091949819	5.324604074466918	21.053911707691743	4.8368750228594415
8	69.25149043560953	5.410189824805237	20.111919827365497	5.226399912219743
9	63.086573278226844	6.96371749387367	22.336417834022164	7.6132913938773275
10	32.41157967887056	23.306023920119966	29.12676932080026	15.15562708020921
11	24.173951208807285	21.665630371968838	35.14447167257964	19.015946746644232
12	21.135839947331846	19.55689257891079	39.0856223254453	20.22164514831206
13	20.373431842288138	20.752532826158514	40.60147763432208	18.272557697231264
14	18.171244650890603	23.79503310047182	38.31205881277203	19.72166343586555
15	17.17402435902125	22.826524267583483	40.89407849017959	19.105372883215683
16	18.922314472769834	22.973007571047148	37.94941662704364	20.15526132913939
17	18.796313229216196	23.912804944954463	36.04659668629531	21.244285139534032
18	18.996379064408764	23.084927398412642	37.28502980871219	20.633663728466406
19	19.15273764675762	25.173914633700306	35.03072308986504	20.64262462967704
20	19.549211806444532	25.132584762810435	35.32551845214147	19.992684978603563
21	19.28715116491716	25.78654767565195	35.69273252624264	19.233568633188252
22	18.622398595515893	23.03445375077722	35.195311071284884	23.147836582422006
23	18.295417139095132	24.733001719030028	35.57331480194579	21.398266339929044
24	19.439120734428148	23.47353791009839	35.99264840349658	21.094692951976885
25	19.130975458103215	26.040196042573427	33.76266413079258	21.066164368530778
26	18.54028748034088	25.63750411469953	34.47661021908489	21.345598185874692
27	20.296441242090633	23.669580483522914	34.30452434073369	21.729453933652756
28	18.633188252075637	24.435097472660107	35.123258110529974	21.808456164734284
29	19.125123440986062	24.35390073515965	33.71676237152993	22.804213452324348
30	19.351523353205806	25.042975750704073	33.30602392011996	22.299476975970155
31	18.79100983870378	25.17354888263048	32.37866208258659	23.656779196079146
32	18.91719395779233	25.64061299879302	31.76548041403021	23.67671262938444
33	18.41209173036831	26.114992136352	30.467795618302183	25.00512051497751
34	17.55714860465967	26.247942650232254	31.14827548370579	25.04663326140229
35	18.212574521780475	25.799531838630628	29.419735927727586	26.568157711861307
36	18.529863574850957	28.235982590249076	29.364507516184485	23.869646318715482
37	17.818294868512492	27.342269851139317	30.631103470977656	24.20833180937054
38	17.764895212318496	27.5476390768443	30.345634760981678	24.341830949855527
39	19.82498811309023	26.789802860173367	29.34000219450642	24.045206832229983
40	19.779634980432316	25.590505102227425	31.28451775721444	23.345342160125817
41	18.089316411250504	27.07819757872792	29.72129768479573	25.111188325225854
42	18.867817563366373	27.46534508613438	30.845250722358365	22.82158662814089
43	19.838155151603818	25.683588749497094	31.72213891225632	22.756117186642772
44	20.27029004059837	25.24176145715226	31.101825097838407	23.38612340441096
45	19.89082330565817	24.253136315423724	30.67133608865806	25.18470429026005
46	20.972897845726198	25.265352401155774	30.31692330200066	23.444826451117372
47	18.99985369957207	25.04078124428514	32.2634504955927	23.69591456055009
48	18.807651512380673	24.419187301122854	31.713909513185328	25.059251673311145
49	19.73501334991405	23.952123184960318	33.00775392268022	23.30510954244541
50	18.5891152481621	23.135949672652792	33.521268424710144	24.753666654474966
51	18.457444863026222	23.28133572290699	31.837167623715303	26.424051790351488
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	306.0
1	561.0
2	816.0
3	12713.5
4	24611.0
5	15333.0
6	6055.0
7	5957.5
8	5860.0
9	5819.5
10	5779.0
11	5678.5
12	5578.0
13	5544.5
14	5511.0
15	5275.5
16	5040.0
17	4698.5
18	4357.0
19	4181.0
20	4005.0
21	3782.0
22	3559.0
23	3407.0
24	3255.0
25	3624.0
26	4330.5
27	4668.0
28	5289.5
29	5911.0
30	6717.0
31	7523.0
32	8599.5
33	9676.0
34	11112.5
35	12549.0
36	12980.0
37	13411.0
38	14717.0
39	16023.0
40	17892.5
41	19762.0
42	21973.5
43	24185.0
44	27561.0
45	30937.0
46	40665.0
47	50393.0
48	52763.5
49	55134.0
50	52183.0
51	49232.0
52	42402.5
53	35573.0
54	31162.5
55	26752.0
56	24185.0
57	21618.0
58	20172.0
59	18726.0
60	17639.5
61	16553.0
62	15163.5
63	13774.0
64	12188.5
65	10603.0
66	9126.5
67	7650.0
68	6577.5
69	5505.0
70	4728.0
71	3951.0
72	3597.0
73	3243.0
74	2713.5
75	1708.5
76	1233.0
77	961.5
78	690.0
79	495.5
80	301.0
81	243.5
82	186.0
83	132.0
84	78.0
85	60.5
86	43.0
87	29.0
88	15.0
89	12.5
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	546820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.90683306420689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01373986180064	30.27667700984314
2	8.644633232987614	7.072491371262389
3	3.7728984247178716	4.630119780844294
4	2.420003817994502	3.9597876878977765
5	1.8764203002497049	3.837920599030183
6	1.5299378059548208	3.755094625608769
7	1.274561770210234	3.6496799894807053
8	1.084868098049683	3.550281454688161
9	0.9297080322727221	3.422827014716926
>10	4.3718893256492395	25.519353994158255
>50	0.04628702211108739	1.3061166244103644
>100	0.0256151481552668	1.8726826251335469
>500	0.004493885641274878	1.238466952539973
>1k	0.004493885641274878	2.6015710720418133
>5k	0.0	0.0
>10k+	4.493885641274878E-4	3.3069291983437097
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17989	3.289747997512893	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	2098	0.38367287224315133	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	1829	0.33447935335210854	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	1656	0.30284188581251603	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	1564	0.28601733660070955	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	1345	0.24596759445521377	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	1259	0.23024029845287297	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	1167	0.21341574924106654	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	1165	0.21304999817124468	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	1054	0.19275081379613035	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	1015	0.1856186679346037	No Hit
CGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	877	0.16038184411689405	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	849	0.15526132913938773	No Hit
CGTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	843	0.1541640759299221	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	718	0.13130463406605464	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTC	625	0.11429720931933726	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	600	0.10972532094656376	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT	589	0.10771369006254343	No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	571	0.1044219304341465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22073077063750413	0.0
2	0.0	0.0	0.0	0.8337295636589737	0.0
3	0.0	0.0	0.0	1.2971361691232948	0.0
4	0.0	0.0	0.0	2.032844446070005	0.0
5	0.0	0.0	0.0	3.925423356863319	0.0
6	0.0	0.0	0.0	5.627080209209612	0.0
7	0.0	0.0	0.0	6.983102300574229	0.0
8	0.0	0.0	0.0	8.784974946051717	0.0
9	0.0	0.0	0.0	9.749826268241835	0.0
10	0.0	0.0	0.0	11.345232434804872	0.0
11	0.0	0.0	0.0	12.883032807870963	0.0
12	0.0	0.0	0.0	14.23704326835156	0.0
13	0.0	0.0	0.0	14.807797812808602	0.0
14	0.0	0.0	0.0	15.085951501408141	0.0
15	0.0	0.0	0.0	15.442375918949564	0.0
16	0.0	0.0	0.0	15.979481364982993	0.0
17	0.0	0.0	0.0	16.638381917267107	0.0
18	0.0	0.0	0.0	17.392560623239824	0.0
19	0.0	0.0	0.0	17.867305511868622	3.657510698218792E-4
20	0.0	0.0	0.0	18.310412932957828	3.657510698218792E-4
21	0.0	0.0	0.0	18.80490837935701	3.657510698218792E-4
22	0.0	0.0	0.0	19.31110785999049	3.657510698218792E-4
23	0.0	0.0	0.0	19.835046267510332	3.657510698218792E-4
24	0.0	0.0	0.0	20.24779634980432	3.657510698218792E-4
25	0.0	0.0	0.0	20.630006217768187	3.657510698218792E-4
26	0.0	0.0	0.0	20.987710764053986	3.657510698218792E-4
27	0.0	0.0	0.0	21.36041110420248	3.657510698218792E-4
28	0.0	0.0	0.0	21.71299513551077	3.657510698218792E-4
29	0.0	0.0	0.0	22.10215427380125	3.657510698218792E-4
30	0.0	0.0	0.0	22.56318349731173	3.657510698218792E-4
31	0.0	0.0	0.0	22.94301598332175	3.657510698218792E-4
32	0.0	0.0	0.0	23.313338941516403	3.657510698218792E-4
33	0.0	0.0	0.0	23.670129110127647	3.657510698218792E-4
34	0.0	0.0	0.0	24.02088438608683	3.657510698218792E-4
35	0.0	0.0	0.0	24.38498957609451	3.657510698218792E-4
36	0.0	0.0	0.0	24.711056654840714	3.657510698218792E-4
37	0.0	0.0	0.0	25.062726308474453	3.657510698218792E-4
38	0.0	0.0	0.0	25.39921729271058	3.657510698218792E-4
39	0.0	0.0	0.0	25.730222010899382	3.657510698218792E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGCC	20	7.030038E-4	45.000004	23
TCGACGT	20	7.030038E-4	45.000004	26
CGTTTAT	20	7.030038E-4	45.000004	39
GTTAGCG	35	1.2103737E-7	45.0	1
ACTACGG	25	3.888114E-5	45.0	2
ATAACGC	25	3.888114E-5	45.0	11
TCGACGG	35	1.2103737E-7	45.0	2
CGTTTTT	7315	0.0	44.077236	1
GCTACGA	85	0.0	42.35294	10
ACACGAC	65	0.0	41.538464	26
GACACGA	65	0.0	41.538464	25
CACGACC	65	0.0	41.538464	27
AGACACG	65	0.0	41.538464	24
GCGTTAG	50	1.0786607E-9	40.5	1
CTACGAA	90	0.0	40.0	11
ATAACGG	45	1.9250365E-8	40.0	2
TTATAGG	80	0.0	39.375004	2
ATTGCGG	80	0.0	39.375004	2
AACACGT	75	0.0	39.0	41
GTTTTTT	8450	0.0	38.742603	2
>>END_MODULE