##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552004_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	395544
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30103098517485	33.0	31.0	34.0	30.0	34.0
2	32.391122100196185	34.0	31.0	34.0	31.0	34.0
3	32.37682786238699	34.0	31.0	34.0	30.0	34.0
4	35.93780211556742	37.0	35.0	37.0	35.0	37.0
5	35.99080254029893	37.0	35.0	37.0	35.0	37.0
6	35.96269694395566	37.0	35.0	37.0	35.0	37.0
7	36.1299349756285	37.0	36.0	37.0	35.0	37.0
8	35.99008959812309	37.0	35.0	37.0	35.0	37.0
9	37.86799445826507	39.0	38.0	39.0	35.0	39.0
10	37.56301700948567	39.0	37.0	39.0	35.0	39.0
11	37.485404910705256	39.0	37.0	39.0	35.0	39.0
12	37.38042038306737	39.0	37.0	39.0	35.0	39.0
13	37.39698744008252	39.0	37.0	39.0	35.0	39.0
14	38.668661387860766	40.0	38.0	41.0	35.0	41.0
15	38.73148625690189	40.0	38.0	41.0	35.0	41.0
16	38.70580264142548	40.0	38.0	41.0	35.0	41.0
17	38.64598123091236	40.0	38.0	41.0	35.0	41.0
18	38.573117023643384	40.0	38.0	41.0	35.0	41.0
19	38.4771656250632	40.0	37.0	41.0	34.0	41.0
20	38.399285540925916	40.0	37.0	41.0	34.0	41.0
21	38.27096353376616	40.0	37.0	41.0	34.0	41.0
22	38.27606031187428	40.0	37.0	41.0	34.0	41.0
23	38.22998199947414	40.0	37.0	41.0	34.0	41.0
24	38.16411322128511	40.0	37.0	41.0	34.0	41.0
25	37.95605545779989	40.0	36.0	41.0	34.0	41.0
26	37.97167192524726	40.0	36.0	41.0	34.0	41.0
27	37.962838521125335	40.0	36.0	41.0	34.0	41.0
28	37.87121533887507	40.0	36.0	41.0	34.0	41.0
29	37.81636177011913	40.0	36.0	41.0	33.0	41.0
30	37.65698632769047	40.0	36.0	41.0	33.0	41.0
31	37.629838905406224	40.0	36.0	41.0	33.0	41.0
32	37.502927613615675	40.0	35.0	41.0	33.0	41.0
33	37.4070166656554	40.0	35.0	41.0	33.0	41.0
34	37.293302388609106	40.0	35.0	41.0	33.0	41.0
35	37.17171288149991	40.0	35.0	41.0	33.0	41.0
36	37.05002730416844	40.0	35.0	41.0	32.0	41.0
37	36.99460489857007	39.0	35.0	41.0	32.0	41.0
38	36.87898691422446	39.0	35.0	41.0	32.0	41.0
39	36.791449244584676	39.0	35.0	41.0	31.0	41.0
40	36.6985594522986	39.0	35.0	41.0	31.0	41.0
41	36.61876049187954	39.0	35.0	41.0	31.0	41.0
42	36.510153105596345	39.0	35.0	41.0	31.0	41.0
43	36.37518455595332	39.0	35.0	41.0	31.0	41.0
44	36.353179418724594	39.0	35.0	41.0	31.0	41.0
45	36.271909067006455	39.0	35.0	41.0	31.0	41.0
46	36.18915973949801	38.0	35.0	41.0	31.0	41.0
47	36.13798717715349	38.0	35.0	41.0	31.0	41.0
48	36.04244534109985	38.0	35.0	40.0	31.0	41.0
49	35.95618945047833	38.0	35.0	40.0	30.0	41.0
50	35.82493982970289	37.0	35.0	40.0	30.0	41.0
51	34.91820379022308	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	8.0
11	7.0
12	5.0
13	11.0
14	13.0
15	25.0
16	42.0
17	64.0
18	104.0
19	198.0
20	274.0
21	506.0
22	783.0
23	1099.0
24	1515.0
25	2293.0
26	3299.0
27	3819.0
28	4202.0
29	4714.0
30	5259.0
31	6324.0
32	8227.0
33	11443.0
34	22394.0
35	31389.0
36	31033.0
37	45613.0
38	76342.0
39	134381.0
40	153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63152519062355	20.07513702647493	24.229921323544286	14.063416459357239
2	33.51081042816981	24.61723600914184	24.9514592561131	16.920494306575247
3	31.75904576987642	23.566783973464396	27.478864551099246	17.19530570555994
4	29.33731771939405	24.995954938009422	26.48074550492486	19.18598183767166
5	25.672491555933096	29.458922395485708	25.23183261533483	19.636753433246366
6	27.18104686204316	34.39592055498251	23.413577250571365	15.009455332402968
7	78.35714863580284	6.790142183928968	10.250945533240296	4.601763647027891
8	79.28852415913273	6.609631292599559	9.417409946807435	4.684434601460268
9	72.80454260461542	8.427886657363024	11.606041300082923	7.161529437938636
10	42.03426167506017	24.505743988026616	18.017717371518717	15.442276965394495
11	32.113999959549375	23.961683150294277	24.058511821693667	19.865805068462674
12	26.988400784742026	22.011204821713893	29.96581922617964	21.034575167364437
13	24.89482838824505	22.81136864672451	31.4233056246587	20.87049734037174
14	22.383603341221203	25.583500192140445	29.650557207289204	22.38233925934915
15	22.25618388851809	23.642881702162086	32.494488603037844	21.606445806281982
16	24.6463098921991	23.283376817749733	30.005005764213337	22.06530752583783
17	24.569959347126993	23.46338207633032	27.76833929979977	24.198319276742914
18	25.161044030499767	23.289697227110008	28.624628359929616	22.924630382460613
19	25.895728414537956	23.87294460287604	27.10596039884311	23.125366583742895
20	26.721932326112896	24.588667758833406	27.451054749914043	21.238345165139656
21	26.200877778451954	24.648079606819977	27.394929514794814	21.756113099933255
22	25.015421798839064	23.28185991950327	26.78387233784358	24.918845943814087
23	25.24826567967154	24.210454462714644	27.3969520457901	23.144327811823718
24	24.8359221730073	24.100984972594706	27.969328317456466	23.093764536941528
25	24.882693202273327	24.772212446655743	27.037194345003336	23.30790000606759
26	23.84336508706996	25.637097263515564	27.031126752017475	23.488410897397003
27	25.140060271423657	24.17227919017859	27.896517201626114	22.791143336771636
28	24.164189066197437	24.685749246607205	28.09472523916429	23.055336448031067
29	25.455827923062923	24.322452116578685	26.724460489857005	23.497259470501387
30	24.915306514572336	25.34003802358271	26.41476043120361	23.329895030641346
31	26.691594361183586	25.26924943874765	24.57071779625023	23.46843840381854
32	25.713447808587663	25.88687984143357	25.702071071739173	22.697601278239592
33	24.669821815019315	25.369617539388788	25.85148554901604	24.109075096575854
34	24.35026191776389	25.181774973201463	26.811682139028783	23.656280970005866
35	25.643417672875835	23.87850656311308	27.049835163723884	23.4282406002872
36	25.621675464676496	26.523724288574723	25.308941609530166	22.545658637218615
37	24.89053050988006	26.399844265113366	26.933539631494853	21.77608559351172
38	24.937301539146087	26.600580466395645	26.138937766721277	22.32318022773699
39	25.180258074955	25.01036547135085	27.389367554557776	22.420008899136377
40	24.158879922334812	24.352284448759175	29.376757073802157	22.112078555103857
41	22.911231114616832	24.76260542442813	27.45838642477196	24.86777703618308
42	23.57436846469672	24.81721376130089	27.725360516149912	23.883057257852478
43	24.57400440911757	23.105899722913254	27.87022429868738	24.4498715692818
44	24.57451004186639	24.19199886738264	26.702465465283254	24.53102562546771
45	24.49891794591752	23.318265477418443	27.1934348643893	24.989381712274742
46	24.06483223105394	25.30793034403252	27.19191796614283	23.435319458770707
47	23.804431365410675	24.30703031773962	28.790981534292015	23.097556782557692
48	23.083651881965093	24.295147948142308	28.451701959832537	24.16949821006007
49	23.720243512731834	24.259248022975953	28.95227838116619	23.068230083126025
50	22.651083065347976	24.803814493457114	28.7143781728455	23.830724268349414
51	23.004267540400054	24.615971927269786	27.181552494791983	25.198208037538176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	252.0
1	566.5
2	881.0
3	2588.5
4	4296.0
5	2815.0
6	1334.0
7	1337.5
8	1341.0
9	1312.0
10	1283.0
11	1274.0
12	1265.0
13	1278.5
14	1292.0
15	1301.0
16	1310.0
17	1266.5
18	1223.0
19	1210.5
20	1198.0
21	1182.0
22	1166.0
23	1335.0
24	1504.0
25	1862.5
26	2761.0
27	3301.0
28	3970.5
29	4640.0
30	5370.5
31	6101.0
32	6552.0
33	7003.0
34	7640.5
35	8278.0
36	8751.5
37	9225.0
38	9919.0
39	10613.0
40	12027.0
41	13441.0
42	15032.5
43	16624.0
44	18670.0
45	20716.0
46	24520.0
47	28324.0
48	29972.0
49	31620.0
50	30713.0
51	29806.0
52	28042.0
53	26278.0
54	24525.0
55	22772.0
56	21998.0
57	21224.0
58	21188.5
59	21153.0
60	20834.5
61	20516.0
62	19761.5
63	19007.0
64	17269.5
65	15532.0
66	13387.5
67	11243.0
68	10167.5
69	9092.0
70	7747.5
71	6403.0
72	5492.5
73	4582.0
74	3804.0
75	2517.5
76	2009.0
77	1657.0
78	1305.0
79	986.0
80	667.0
81	482.0
82	297.0
83	201.0
84	105.0
85	85.5
86	66.0
87	36.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	395544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.078794126157256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.12284859338926	22.4945642356703
2	7.350599784543782	4.715967543843117
3	3.318606376337145	3.1937067219681605
4	2.3270147437000457	2.9859130756674546
5	1.8514252014023065	2.969574393788191
6	1.6945791536343715	3.261603347994888
7	1.4749314283670638	3.3119815148550273
8	1.3735931833092498	3.525057035237633
9	1.2399846999681419	3.5799492518876606
>10	9.150001274377493	45.188484046306364
>50	0.05927186125273187	1.3314659161762736
>100	0.034772825268269365	2.456316310325955
>500	0.0015805829667395164	0.30066994984483253
>1k	7.902914833697582E-4	0.6847466564341422
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2701	0.6828570272839432	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCC	638	0.16129684687417833	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	548	0.13854337317719395	TruSeq Adapter, Index 22 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	489	0.12362720708694862	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	456	0.11528426673138766	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	442	0.11174483748963454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCG	441	0.11149202111522358	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	411	0.10390752988289546	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09632303865056732	0.0
2	0.0	0.0	0.0	0.41183787391541776	0.0
3	0.0	0.0	0.0	0.6032198693444977	0.0
4	0.0	0.0	0.0	0.8977509455332403	0.0
5	0.0	0.0	0.0	1.741146370568129	0.0
6	0.0	0.0	0.0	2.301387456262767	0.0
7	0.0	0.0	0.0	2.813846247193738	0.0
8	0.0	0.0	0.0	3.615274154076411	0.0
9	0.0	0.0	0.0	4.010173330906296	0.0
10	2.5281637441093785E-4	0.0	0.0	4.787836498594341	0.0
11	2.5281637441093785E-4	0.0	0.0	5.660052990312076	0.0
12	2.5281637441093785E-4	0.0	0.0	6.374764880771798	0.0
13	2.5281637441093785E-4	0.0	0.0	6.6445199522682685	0.0
14	2.5281637441093785E-4	0.0	0.0	6.762332382743765	0.0
15	2.5281637441093785E-4	0.0	0.0	6.993153732580952	0.0
16	2.5281637441093785E-4	0.0	0.0	7.393109236899056	0.0
17	2.5281637441093785E-4	0.0	0.0	7.84185830147847	0.0
18	2.5281637441093785E-4	0.0	0.0	8.377070566106426	0.0
19	2.5281637441093785E-4	0.0	0.0	8.679186133527496	0.0
20	2.5281637441093785E-4	0.0	0.0	8.986105212062375	0.0
21	2.5281637441093785E-4	0.0	0.0	9.325384786521854	0.0
22	2.5281637441093785E-4	0.0	0.0	9.68868191655037	0.0
23	2.5281637441093785E-4	0.0	0.0	10.05754100681593	0.0
24	2.5281637441093785E-4	0.0	0.0	10.36218473798111	0.0
25	2.5281637441093785E-4	0.0	0.0	10.651406670307223	0.0
26	2.5281637441093785E-4	0.0	0.0	10.904981493841394	0.0
27	2.5281637441093785E-4	0.0	0.0	11.162095746617316	0.0
28	2.5281637441093785E-4	0.0	0.0	11.430839552616144	0.0
29	2.5281637441093785E-4	0.0	0.0	11.70211152235908	0.0
30	2.5281637441093785E-4	0.0	0.0	12.013328479258945	0.0
31	2.5281637441093785E-4	0.0	0.0	12.306595473575632	0.0
32	2.5281637441093785E-4	0.0	0.0	12.593036425783225	0.0
33	5.056327488218757E-4	0.0	0.0	12.8744210505026	0.0
34	5.056327488218757E-4	0.0	0.0	13.154035960601096	0.0
35	5.056327488218757E-4	0.0	0.0	13.441235361931922	0.0
36	5.056327488218757E-4	0.0	0.0	13.728181946888336	0.0
37	5.056327488218757E-4	0.0	0.0	14.041168618409078	0.0
38	5.056327488218757E-4	0.0	0.0	14.329884917986368	0.0
39	5.056327488218757E-4	0.0	0.0	14.614808971947495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	25	3.88615E-5	45.0	4
TCTACTA	20	7.0276705E-4	45.0	24
TAACCCC	20	7.0276705E-4	45.0	25
ATTTGCG	20	7.0276705E-4	45.0	1
TTATAGG	45	1.9228537E-8	40.000004	2
TAACCGG	40	3.45055E-7	39.375	2
GCGATCA	40	3.45055E-7	39.375	9
CCCCACG	80	0.0	39.375	38
CGTTTTT	1215	0.0	39.25926	1
CGTTAGG	35	6.237902E-6	38.571426	2
TACGGGC	90	0.0	37.500004	4
ATACTGG	60	1.546141E-10	37.499996	2
TCTACGG	30	1.1385685E-4	37.499996	2
GCGTAAG	30	1.1385685E-4	37.499996	1
CACGAGC	85	0.0	37.058823	41
GTGGCGT	55	2.739398E-9	36.81818	34
CTACGAA	55	2.739398E-9	36.81818	11
CGAATAT	55	2.739398E-9	36.81818	14
GCTACGA	55	2.739398E-9	36.81818	10
ACAACGA	25	0.0021054414	36.0	13
>>END_MODULE