FastQCFastQC Report
Sat 18 Jun 2016
SRR3552002_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552002_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438509
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCG44811.0218718429952407No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGC35870.8179991744753243No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTC33110.7550586190933367No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28590.6519820573808064No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC17160.3913260617227925No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCT9250.21094207872586423No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCC8620.196575212823454No Hit
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC8200.1869973022218472No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT7660.17468284573406703No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTC6920.15780747943599788No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGGTCAT6770.15438679707828118No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCT6760.15415875158776673No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGGTCATCGTA6160.14047602215689986No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC5270.12017997350111399No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4690.10695333505127602No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4610.10512897112716045No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4590.10467288014613155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAT351.209628E-745.0000046
GCGATAT351.209628E-745.0000049
TTACGAG302.16242E-645.0000041
TCACCGT207.0285104E-445.015
CGACGAA207.0285104E-445.019
TATAGCG207.0285104E-445.01
ATTATCC253.886846E-545.045
ATCGCCA207.0285104E-445.024
GCATATT406.7975634E-945.027
TCGTTCA556.002665E-1140.90909216
GGTATGC556.002665E-1140.9090928
CGAATAT501.0786607E-940.514
GATGAAT3700.040.13513620
TTGCGAG451.9235813E-840.01
GCGATAC451.9235813E-840.09
TGATTCG403.451696E-739.37515
CGATGAA3200.039.37519
TGCGTAG403.451696E-739.3751
TAATCGT403.451696E-739.37521
CGTTTTT14500.039.1034471