##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552001_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411575
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35902326428962	33.0	31.0	34.0	30.0	34.0
2	32.4330365061046	34.0	31.0	34.0	31.0	34.0
3	32.437553301342405	34.0	31.0	34.0	31.0	34.0
4	35.97563749012938	37.0	35.0	37.0	35.0	37.0
5	36.04562959363421	37.0	35.0	37.0	35.0	37.0
6	36.044934702059166	37.0	35.0	37.0	35.0	37.0
7	36.21791654012027	37.0	37.0	37.0	35.0	37.0
8	36.06503796391909	37.0	37.0	37.0	35.0	37.0
9	37.957949340946364	39.0	38.0	39.0	35.0	39.0
10	37.64885622304562	39.0	37.0	39.0	35.0	39.0
11	37.632382919273525	39.0	37.0	39.0	35.0	39.0
12	37.5535151551965	39.0	37.0	39.0	35.0	39.0
13	37.55684140193161	39.0	37.0	39.0	35.0	39.0
14	38.83461823482962	40.0	38.0	41.0	35.0	41.0
15	38.89529247403268	40.0	38.0	41.0	35.0	41.0
16	38.90352183684626	40.0	38.0	41.0	35.0	41.0
17	38.86031707465225	40.0	38.0	41.0	35.0	41.0
18	38.78213934276863	40.0	38.0	41.0	35.0	41.0
19	38.71727388689789	40.0	38.0	41.0	35.0	41.0
20	38.635782056733284	40.0	38.0	41.0	35.0	41.0
21	38.54594423859564	40.0	38.0	41.0	35.0	41.0
22	38.565058616291076	40.0	38.0	41.0	35.0	41.0
23	38.53356739354917	40.0	38.0	41.0	35.0	41.0
24	38.47468869586345	40.0	38.0	41.0	35.0	41.0
25	38.29973151916418	40.0	37.0	41.0	34.0	41.0
26	38.33324181497905	40.0	37.0	41.0	34.0	41.0
27	38.327738565267566	40.0	37.0	41.0	34.0	41.0
28	38.258752353762986	40.0	37.0	41.0	34.0	41.0
29	38.22340035230517	40.0	37.0	41.0	34.0	41.0
30	38.098254267144505	40.0	37.0	41.0	34.0	41.0
31	38.03112676911863	40.0	37.0	41.0	34.0	41.0
32	37.92595031282269	40.0	37.0	41.0	34.0	41.0
33	37.82627224685659	40.0	37.0	41.0	34.0	41.0
34	37.71702848812489	40.0	36.0	41.0	33.0	41.0
35	37.6341930389358	40.0	36.0	41.0	33.0	41.0
36	37.5151503371196	40.0	36.0	41.0	33.0	41.0
37	37.48580453137338	40.0	36.0	41.0	33.0	41.0
38	37.365308874445724	40.0	35.0	41.0	33.0	41.0
39	37.33904877604325	40.0	35.0	41.0	33.0	41.0
40	37.266692583368766	40.0	35.0	41.0	33.0	41.0
41	37.15960396039604	40.0	35.0	41.0	33.0	41.0
42	37.04410374779809	39.0	35.0	41.0	32.0	41.0
43	36.91450889874263	39.0	35.0	41.0	32.0	41.0
44	36.9716236408917	39.0	35.0	41.0	32.0	41.0
45	36.9654497965134	39.0	35.0	41.0	32.0	41.0
46	36.89570309178157	39.0	35.0	41.0	32.0	41.0
47	36.80942234100711	39.0	35.0	41.0	32.0	41.0
48	36.749656806171416	39.0	35.0	41.0	32.0	41.0
49	36.697044281115225	39.0	35.0	41.0	32.0	41.0
50	36.61087772580939	39.0	35.0	41.0	32.0	41.0
51	35.78968596246128	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	6.0
11	8.0
12	7.0
13	10.0
14	12.0
15	18.0
16	28.0
17	54.0
18	78.0
19	126.0
20	260.0
21	453.0
22	592.0
23	917.0
24	1295.0
25	2000.0
26	2756.0
27	3256.0
28	3594.0
29	3739.0
30	4613.0
31	5777.0
32	7211.0
33	10372.0
34	20130.0
35	29039.0
36	27923.0
37	42368.0
38	81150.0
39	163575.0
40	203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81947397193707	19.211079390147603	24.136062686023205	12.833383951892122
2	33.49960517524145	24.18004008989856	25.065176456295934	17.255178278564053
3	32.66549231610277	24.01360626860232	26.956447792018466	16.364453623276436
4	28.936645811820444	24.74494320597704	27.192856708983783	19.125554273218732
5	26.084917694223407	28.480349875478346	24.542064022353156	20.892668407945088
6	26.17894672902873	34.1918240903845	24.26337848508777	15.365850695498997
7	77.94715422462491	6.3606876025025825	10.656866913685233	5.035291259187268
8	78.22875539087651	6.6381582943570425	9.826155621697138	5.306930693069307
9	71.60639008686145	8.359837210714936	12.440259976917938	7.59351272550568
10	36.799854218550685	28.14456660389965	18.781510052845775	16.274069124703882
11	29.86964708740813	24.655044645568854	25.74986332989127	19.72544493713175
12	27.030067423920308	21.948369070035838	30.472939318471724	20.54862418757213
13	25.099678065966106	23.615136973820082	31.498268845289434	19.786916114924377
14	21.4957176699265	27.631658871408614	28.781145599222498	22.091477859442385
15	19.654497965133938	25.056672538419487	34.138127923221774	21.150701573224808
16	22.77106238231185	23.81704428111523	31.248010690639617	22.163882645933306
17	22.4748830711292	23.999271092753446	29.05910222924133	24.466743606876026
18	23.26793415537873	23.841827127498025	30.248678855615623	22.641559861507623
19	24.052238352669622	24.79645265140011	28.35449189090688	22.796817105023386
20	25.541152888294967	25.13563749012938	28.81346048715301	20.509749134422645
21	24.94029034805321	25.777804774342467	27.97788981352123	21.304015064083096
22	23.09542610702788	24.47986393731398	27.383830407580632	25.040879548077505
23	22.839822632570005	25.40338941869647	28.05709773431331	23.699690214420215
24	22.78345380550325	24.78017372289376	29.068578023446516	23.367794448156474
25	22.399076717487702	26.559436311729336	26.82232885865274	24.21915811213023
26	21.632752232278442	27.46668286460548	27.765048897527787	23.13551600558829
27	23.97473121545283	26.052359837210716	27.814128652129018	22.158780295207432
28	21.1492437587317	27.136487881917027	28.49079754601227	23.223470813339002
29	22.32302739476402	26.517159691429264	28.41207556338456	22.74773735042216
30	24.703881431087893	25.50980987669319	27.658871408613255	22.127437283605662
31	24.501245216546195	27.754358257911683	25.23355403024965	22.510842495292476
32	24.825608941262225	27.515762619206708	26.65492316102776	21.00370527850331
33	25.310089291137704	25.999878515458906	25.992346473911194	22.697685719492195
34	23.627285427929294	26.80507805381765	27.176820749559617	22.390815768693432
35	25.088501488185628	25.654376480592845	26.765717062503796	22.49140496871773
36	25.589260766567456	27.36487881917026	26.332260219887022	20.713600194375264
37	24.648970418514242	27.16953167709409	27.52159387717913	20.659904027212537
38	23.559740023082064	27.09275344712385	27.192370770819412	22.15513575897467
39	25.60311000425196	24.120512664763407	28.298366032922313	21.978011298062324
40	26.23944603049262	24.131689242543885	28.674482172143595	20.9543825548199
41	23.389661665553056	25.158476583854707	28.343558282208587	23.10830346838365
42	23.56800097187633	26.444268966773976	28.63900868614469	21.348721375205002
43	23.874142015428536	24.71943145234769	28.895827006013487	22.51059952621029
44	23.715726173844377	25.084856951952865	28.366154406851727	22.83326246735103
45	24.224503431938285	24.122942355585252	27.19577233796999	24.45678187450647
46	25.14705703699204	25.718277349207312	26.991921278017372	22.14274433578327
47	23.437040636578995	25.644900686387658	29.497661422583977	21.420397254449373
48	23.45210471967442	24.780416691975944	28.803741723865638	22.963736864483995
49	23.46036566846869	23.67685112069489	29.78217821782178	23.08060499301464
50	22.107270849784367	24.26094879426593	29.263196258276135	24.36858409767357
51	22.463463524266537	23.65960031585981	27.682682378667316	26.19425378120634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	293.0
1	541.0
2	789.0
3	2137.0
4	3485.0
5	2308.0
6	1131.0
7	1124.5
8	1118.0
9	1145.0
10	1172.0
11	1143.0
12	1114.0
13	1141.0
14	1168.0
15	1226.5
16	1285.0
17	1170.0
18	1055.0
19	1182.5
20	1310.0
21	1338.5
22	1367.0
23	1532.0
24	1697.0
25	2221.5
26	3316.5
27	3887.0
28	4281.5
29	4676.0
30	5559.5
31	6443.0
32	6927.0
33	7411.0
34	8377.5
35	9344.0
36	10406.5
37	11469.0
38	12248.5
39	13028.0
40	14805.0
41	16582.0
42	18031.5
43	19481.0
44	21624.0
45	23767.0
46	28982.5
47	34198.0
48	36888.5
49	39579.0
50	38945.0
51	38311.0
52	34535.5
53	30760.0
54	27499.5
55	24239.0
56	22552.0
57	20865.0
58	20182.5
59	19500.0
60	18692.0
61	17884.0
62	15998.0
63	14112.0
64	12696.5
65	11281.0
66	9185.5
67	7090.0
68	6390.0
69	5690.0
70	5035.0
71	4380.0
72	3788.5
73	3197.0
74	2601.5
75	1657.5
76	1309.0
77	1013.0
78	717.0
79	529.5
80	342.0
81	271.0
82	200.0
83	134.0
84	68.0
85	43.0
86	18.0
87	10.5
88	3.0
89	3.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.458042130342164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.53825683319025	24.9952631001965
2	8.601979568187526	5.928147487298966
3	3.23734334946697	3.3465754057894768
4	1.9564781342795885	2.696656239123972
5	1.5403922824274585	2.6539451082569645
6	1.2127926171710577	2.507427545870895
7	1.106412789795135	2.6687372967017122
8	0.9825783745084508	2.7086181620140257
9	0.9257588978933504	2.8709855195533382
>10	7.784251189868521	43.340021848768046
>50	0.07065587777124399	1.6324416843150464
>100	0.03674105644104688	2.472887425441898
>500	0.00423935266627464	1.1250578707262984
>1k	0.00211967633313732	1.0532353059428623
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2128	0.5170382068881735	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1194	0.2901050841280447	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1004	0.24394095851302924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCG	942	0.22887687541760313	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCC	842	0.20457996719917393	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	834	0.20263621454169956	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGC	766	0.1861143169531677	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTC	731	0.1776103990767175	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	506	0.12294235558525178	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	482	0.11711109761282877	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	435	0.10569155075016703	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21016825608941261	0.0
2	0.0	0.0	0.0	0.9361598736560772	0.0
3	0.0	0.0	0.0	1.2746158051387961	0.0
4	0.0	0.0	0.0	1.7423312883435582	0.0
5	0.0	0.0	0.0	3.0436736925226264	0.0
6	0.0	0.0	0.0	3.805138796088198	0.0
7	0.0	0.0	0.0	4.544736682257183	0.0
8	0.0	0.0	0.0	5.628864726963494	0.0
9	0.0	0.0	0.0	6.097552086496993	0.0
10	0.0	0.0	0.0	7.308509992103505	0.0
11	0.0	0.0	0.0	8.771426835935127	0.0
12	0.0	0.0	0.0	9.9597886168985	0.0
13	0.0	0.0	0.0	10.380854036323878	0.0
14	0.0	0.0	0.0	10.565024600619571	0.0
15	0.0	0.0	0.0	10.919516491526453	0.0
16	0.0	0.0	0.0	11.60371742695742	0.0
17	0.0	0.0	0.0	12.398955232946607	0.0
18	0.0	0.0	0.0	13.244973577112312	0.0
19	0.0	0.0	0.0	13.768328980137278	0.0
20	2.4296908218429206E-4	0.0	0.0	14.26325700054668	0.0
21	2.4296908218429206E-4	0.0	0.0	14.820385105995262	0.0
22	2.4296908218429206E-4	0.0	0.0	15.410557006620907	0.0
23	2.4296908218429206E-4	0.0	0.0	15.986150762315495	0.0
24	2.4296908218429206E-4	0.0	0.0	16.441717791411044	0.0
25	2.4296908218429206E-4	0.0	0.0	16.868128530644476	0.0
26	2.4296908218429206E-4	0.0	0.0	17.282390815768693	0.0
27	2.4296908218429206E-4	0.0	0.0	17.682560894126222	0.0
28	2.4296908218429206E-4	0.0	0.0	18.08491769422341	0.0
29	2.4296908218429206E-4	0.0	0.0	18.47658385470449	0.0
30	2.4296908218429206E-4	0.0	0.0	18.93701026544372	0.0
31	2.4296908218429206E-4	0.0	0.0	19.376541335115107	0.0
32	2.4296908218429206E-4	0.0	0.0	19.800036445362327	0.0
33	4.859381643685841E-4	0.0	0.0	20.191702605843407	0.0
34	4.859381643685841E-4	0.0	0.0	20.598189880337728	0.0
35	4.859381643685841E-4	0.0	0.0	21.029945939379214	0.0
36	4.859381643685841E-4	0.0	0.0	21.448824637064934	0.0
37	4.859381643685841E-4	0.0	0.0	21.852396282573043	0.0
38	4.859381643685841E-4	0.0	0.0	22.235315556095486	0.0
39	4.859381643685841E-4	0.0	0.0	22.614347324302983	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTTG	25	3.886427E-5	45.0	10
TACGGGT	20	7.0280035E-4	45.0	4
CTTGCGA	25	3.886427E-5	45.0	12
AAACCGC	25	3.886427E-5	45.0	45
CGAATAT	55	1.8189894E-12	45.0	14
TATAGCG	20	7.0280035E-4	45.0	1
CGGTCAA	20	7.0280035E-4	45.0	31
CGCAACT	20	7.0280035E-4	45.0	8
CCACGCA	20	7.0280035E-4	45.0	5
GATAGCG	20	7.0280035E-4	45.0	1
CGAATAG	60	0.0	44.999996	26
GCGATAT	30	2.1620926E-6	44.999996	9
TCGTAAC	60	0.0	44.999996	34
CGTAACC	60	0.0	44.999996	35
CTCGTAA	65	0.0	41.53846	33
CGGGATA	90	0.0	40.0	6
TCGGCGT	120	0.0	39.374996	4
ACACGAC	115	0.0	39.130436	26
CACGACC	115	0.0	39.130436	27
TAGGGTA	115	0.0	39.130436	5
>>END_MODULE