FastQCFastQC Report
Sat 18 Jun 2016
SRR3552000_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552000_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391557
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34760.8877379283220579No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC14680.37491348641449396TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT12740.32536769870031695TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC12270.31336433775925343No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC11690.29855167957666445TruSeq Adapter, Index 19 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC7070.18056119543259347TruSeq Adapter, Index 19 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC5810.14838197248421048No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG5780.14761580050924897No Hit
ACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC5050.12897228245185247TruSeq Adapter, Index 16 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4340.11083954571109698No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGCG207.027582E-445.0000041
CATTGCG207.027582E-445.0000041
ATATGCG207.027582E-445.0000041
GTATGCG207.027582E-445.0000041
TAGGACG207.027582E-445.0000041
CGTCAGG207.027582E-445.0000042
TAATGCG207.027582E-445.0000041
AACGTAG253.8860795E-545.01
CGCGAGG351.2091914E-745.02
CGAATAT351.2091914E-745.014
TTAAACG253.8860795E-545.01
GGACCTA253.8860795E-545.08
TATACGG253.8860795E-545.02
CCGTTAG253.8860795E-545.031
CGTTTTT15850.042.728711
GCGGGTA556.002665E-1140.9090925
GCAACAG501.0768417E-940.51
TAAACGG451.9226718E-840.02
CGACAGG403.450441E-739.3750042
CTACGAA403.450441E-739.37500411
Produced by FastQC (version 0.11.5)