##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552000_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391557
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.259065729893734	33.0	31.0	34.0	30.0	34.0
2	32.338180648028256	34.0	31.0	34.0	30.0	34.0
3	32.339638928687265	34.0	31.0	34.0	30.0	34.0
4	35.9095533983558	37.0	35.0	37.0	35.0	37.0
5	35.99530336579349	37.0	35.0	37.0	35.0	37.0
6	36.000904082930454	37.0	35.0	37.0	35.0	37.0
7	36.23606524720539	37.0	36.0	37.0	35.0	37.0
8	36.083474436672056	37.0	37.0	37.0	35.0	37.0
9	37.980304272430324	39.0	38.0	39.0	35.0	39.0
10	37.63914576932605	39.0	37.0	39.0	35.0	39.0
11	37.61450567861129	39.0	37.0	39.0	35.0	39.0
12	37.6287513695324	39.0	37.0	39.0	35.0	39.0
13	37.64129871257569	39.0	37.0	39.0	35.0	39.0
14	38.884578745878635	40.0	38.0	41.0	35.0	41.0
15	38.92402383305623	40.0	38.0	41.0	35.0	41.0
16	38.926547092760444	40.0	38.0	41.0	35.0	41.0
17	38.89803272575896	40.0	38.0	41.0	35.0	41.0
18	38.80671268806329	40.0	38.0	41.0	35.0	41.0
19	38.73052454687313	40.0	38.0	41.0	35.0	41.0
20	38.65903048598288	40.0	38.0	41.0	35.0	41.0
21	38.56596613009089	40.0	38.0	41.0	35.0	41.0
22	38.60480083359511	40.0	38.0	41.0	35.0	41.0
23	38.56805267176937	40.0	38.0	41.0	35.0	41.0
24	38.53071966533608	40.0	38.0	41.0	35.0	41.0
25	38.38496055491282	40.0	37.0	41.0	34.0	41.0
26	38.43701427889171	40.0	38.0	41.0	35.0	41.0
27	38.46656553196597	40.0	38.0	41.0	35.0	41.0
28	38.42016360325572	40.0	38.0	41.0	35.0	41.0
29	38.390101568864814	40.0	38.0	41.0	35.0	41.0
30	38.24421987092556	40.0	37.0	41.0	34.0	41.0
31	38.20043825036968	40.0	37.0	41.0	34.0	41.0
32	38.09618522973667	40.0	37.0	41.0	34.0	41.0
33	37.99627129638852	40.0	37.0	41.0	34.0	41.0
34	37.90256080213098	40.0	37.0	41.0	34.0	41.0
35	37.81785027467265	40.0	37.0	41.0	34.0	41.0
36	37.73621465074051	40.0	37.0	41.0	33.0	41.0
37	37.71049681144763	40.0	37.0	41.0	33.0	41.0
38	37.63932709669346	40.0	37.0	41.0	33.0	41.0
39	37.6096072857847	40.0	37.0	41.0	33.0	41.0
40	37.55580413579632	40.0	37.0	41.0	33.0	41.0
41	37.4757008558141	40.0	37.0	41.0	33.0	41.0
42	37.400079681885394	40.0	36.0	41.0	33.0	41.0
43	37.27007306726735	40.0	36.0	41.0	33.0	41.0
44	37.31610723342961	40.0	36.0	41.0	33.0	41.0
45	37.29279517413812	40.0	36.0	41.0	33.0	41.0
46	37.24273349729414	40.0	36.0	41.0	33.0	41.0
47	37.21730935725833	40.0	36.0	41.0	33.0	41.0
48	37.19977168075146	40.0	36.0	41.0	33.0	41.0
49	37.20534430491602	40.0	36.0	41.0	33.0	41.0
50	37.12214313624836	40.0	35.0	41.0	33.0	41.0
51	36.352347167845295	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	4.0
11	0.0
12	7.0
13	3.0
14	5.0
15	8.0
16	12.0
17	26.0
18	56.0
19	120.0
20	160.0
21	275.0
22	398.0
23	729.0
24	1147.0
25	1897.0
26	2887.0
27	3382.0
28	3655.0
29	3756.0
30	4098.0
31	5176.0
32	6515.0
33	9106.0
34	16202.0
35	24352.0
36	24741.0
37	36564.0
38	74638.0
39	171455.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.15047617588244	20.422058601940456	25.780665394821188	13.646799827355915
2	32.61057777028632	24.091256190031082	26.381855004507642	16.916311035174957
3	30.035984543757362	23.900223977607347	29.490214706926448	16.573576771708844
4	27.32194801778541	25.440995819254926	28.880341814857097	18.356714348102578
5	24.266964962955583	28.345042995017327	27.545670234474162	19.842321807552924
6	25.458362384020717	33.593321023503606	25.788582505229122	15.159734087246557
7	78.87893716623633	5.059288941329104	11.433584382350462	4.6281895100841
8	79.0130172618546	4.680033813723162	10.679926549646666	5.627022374775575
9	71.61383910899308	8.069067849636195	13.447084332549283	6.870008708821448
10	30.959477164244287	36.41819709518665	19.02405013829404	13.59827560227502
11	23.962539298237548	23.920144448956346	32.742870131296336	19.374446121509767
12	23.69718840424255	20.59138260840695	34.99030792451674	20.721121062833763
13	23.183342399701704	21.778438388280634	36.88147574938004	18.15674346263762
14	20.24992529823244	25.93492135244677	33.114463539152666	20.700689810168125
15	17.810944511271668	25.528339424400535	36.95707138424291	19.703644680084892
16	20.48462931322898	24.496050383469072	34.198341493064866	20.82097881023708
17	20.220810763183906	24.169405731477152	31.91617057031288	23.693612935026064
18	19.700835382843366	24.4590187380126	33.83287746100823	22.0072684181358
19	19.811674928554464	26.04141925696642	32.4496816555444	21.697224158934713
20	21.395863182116525	26.282763429079292	32.01832683364108	20.303046555163103
21	21.24594886568239	26.357592891967197	32.53344979147353	19.863008450876883
22	19.052143110709295	24.481748506603125	31.111434605944982	25.354673776742594
23	17.694741761735838	26.528704633041933	32.30078890174355	23.475764703478678
24	19.746805701341057	25.171047893410154	32.77326161963648	22.308884785612314
25	18.739034163608366	27.600834616671392	30.367992399574007	23.292138820146235
26	18.3694838810186	27.389115760923698	31.723095232622583	22.518305125435123
27	21.666066498619614	25.132994685320398	31.380616359814788	21.820322456245194
28	18.095449704640703	25.340627290534968	32.52553268106559	24.038390323758737
29	19.51797567148589	23.381780941216732	33.26693176217	23.833311625127376
30	22.439644802672408	23.542677055958645	31.015918499733118	23.00175964163583
31	20.720865672175442	24.705726113950206	28.956192840378286	25.617215373496066
32	19.894166111191986	24.95319966186277	32.09009160862914	23.062542618316108
33	20.757641926973594	23.70178543609232	29.997931335667605	25.54264130126648
34	18.480068036071376	22.439644802672408	32.09877489101204	26.981512270244178
35	19.323112599187347	21.54705445184226	33.751407841003996	25.3784251079664
36	20.599555109473204	22.54307801929221	32.58069706326282	24.276669807971764
37	19.17192132946161	23.565917605865813	32.84221709738301	24.419943967289566
38	17.634980347688842	24.425817952430936	32.1319756765937	25.80722602328652
39	19.235258212725096	22.544865753900453	32.573035343513205	25.646840689861243
40	19.936816351131508	21.959510365029868	33.461539443810224	24.6421338400284
41	18.127884318247407	23.182831618385062	33.17882198504943	25.510462078318096
42	19.83057383727018	23.350367890243312	33.70594830382294	23.11310996866357
43	21.398672479358048	22.86027321692627	32.7275466917971	23.01350761191857
44	20.262184049831824	22.89092009592473	31.250622514729653	25.596273339513786
45	20.51093455103599	22.007779199452443	30.724517758589425	26.756768490922138
46	21.68164532877716	22.518049734776802	31.36963456150701	24.430670374939027
47	18.237702301325225	23.11668543788005	34.01854646960724	24.62706579118749
48	18.93159871998202	22.27772712529721	32.966336957326774	25.824337197393994
49	20.236389593341457	20.921347338957037	34.19808610240654	24.644176965294964
50	18.800583312263605	20.74998020722398	33.37828208919774	27.07115439131467
51	18.628194617897268	20.875377020459347	31.00723521735022	29.489193144293168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161.0
1	334.0
2	507.0
3	2764.0
4	5021.0
5	3220.5
6	1420.0
7	1391.0
8	1362.0
9	1357.5
10	1353.0
11	1391.5
12	1430.0
13	1365.0
14	1300.0
15	1276.0
16	1252.0
17	1186.5
18	1121.0
19	1200.5
20	1280.0
21	1302.0
22	1324.0
23	1614.5
24	1905.0
25	2523.0
26	3537.5
27	3934.0
28	4776.5
29	5619.0
30	5973.5
31	6328.0
32	7242.0
33	8156.0
34	9284.0
35	10412.0
36	11887.0
37	13362.0
38	14279.0
39	15196.0
40	16703.5
41	18211.0
42	20675.5
43	23140.0
44	25275.5
45	27411.0
46	33570.5
47	39730.0
48	43295.5
49	46861.0
50	44302.5
51	41744.0
52	35462.0
53	29180.0
54	24535.0
55	19890.0
56	17368.5
57	14847.0
58	13318.5
59	11790.0
60	10681.0
61	9572.0
62	8733.5
63	7895.0
64	6796.5
65	5698.0
66	4475.5
67	3253.0
68	2688.5
69	2124.0
70	1867.5
71	1611.0
72	1403.5
73	1196.0
74	1009.0
75	652.0
76	482.0
77	355.0
78	228.0
79	209.0
80	190.0
81	133.5
82	77.0
83	46.0
84	15.0
85	9.0
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	391557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.82676498627944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0407274217036	28.691491204648063
2	10.18013538246872	8.108837188121793
3	3.8424382504726804	4.5909565552759855
4	2.293716038859123	3.6540515849960826
5	1.7059274467748013	3.397078575317187
6	1.374033709146902	3.28339905704517
7	1.201672893400283	3.350112074108485
8	1.0357577475828026	3.3000704316558735
9	0.9167760217297212	3.2861000846237234
>10	5.3077561028480575	31.39234099477499
>50	0.06372509246059065	1.7737550364974135
>100	0.03154070232897921	2.355692300964696
>500	0.002574751210528915	0.6078296273356507
>1k	0.003218439013161144	2.208285284634878
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3476	0.8877379283220579	No Hit
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1468	0.37491348641449396	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1274	0.32536769870031695	TruSeq Adapter, Index 16 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	1227	0.31336433775925343	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1169	0.29855167957666445	TruSeq Adapter, Index 19 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	707	0.18056119543259347	TruSeq Adapter, Index 19 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	581	0.14838197248421048	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	578	0.14761580050924897	No Hit
ACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	505	0.12897228245185247	TruSeq Adapter, Index 16 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	434	0.11083954571109698	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.553906583204999E-4	0.0	0.0	0.33302941844993195	0.0
2	2.553906583204999E-4	0.0	0.0	1.3400347842076632	0.0
3	2.553906583204999E-4	0.0	0.0	1.8515822728236246	0.0
4	2.553906583204999E-4	0.0	0.0	2.599621511044369	0.0
5	2.553906583204999E-4	0.0	0.0	4.60239505359373	0.0
6	2.553906583204999E-4	0.0	0.0	5.818820759174271	0.0
7	2.553906583204999E-4	0.0	0.0	7.02375388513039	0.0
8	2.553906583204999E-4	0.0	0.0	8.933565228051089	0.0
9	2.553906583204999E-4	0.0	0.0	9.771246587342327	0.0
10	2.553906583204999E-4	0.0	0.0	11.5349744737037	0.0
11	2.553906583204999E-4	0.0	0.0	13.737463511059692	0.0
12	2.553906583204999E-4	0.0	0.0	15.51396093033709	0.0
13	2.553906583204999E-4	0.0	0.0	16.168782578270854	0.0
14	2.553906583204999E-4	0.0	0.0	16.424939408566313	0.0
15	2.553906583204999E-4	0.0	0.0	16.880300952351764	0.0
16	2.553906583204999E-4	0.0	0.0	17.75680169170772	0.0
17	2.553906583204999E-4	0.0	0.0	18.91653067114111	0.0
18	2.553906583204999E-4	0.0	0.0	20.176117397977816	0.0
19	2.553906583204999E-4	0.0	0.0	20.95480351519702	0.0
20	2.553906583204999E-4	0.0	0.0	21.65023227780374	0.0
21	2.553906583204999E-4	0.0	0.0	22.468248556404305	0.0
22	2.553906583204999E-4	0.0	0.0	23.38459023845826	0.0
23	2.553906583204999E-4	0.0	0.0	24.227124020257587	0.0
24	2.553906583204999E-4	0.0	0.0	24.906718562048436	0.0
25	2.553906583204999E-4	0.0	0.0	25.482369105902844	0.0
26	2.553906583204999E-4	0.0	0.0	26.04678246079115	0.0
27	2.553906583204999E-4	0.0	0.0	26.604811049221443	0.0
28	2.553906583204999E-4	0.0	0.0	27.187867922167143	0.0
29	2.553906583204999E-4	0.0	0.0	27.82506761467679	0.0
30	2.553906583204999E-4	0.0	0.0	28.518708642675268	0.0
31	2.553906583204999E-4	0.0	0.0	29.134966301202635	0.0
32	2.553906583204999E-4	0.0	0.0	29.705764422548953	0.0
33	2.553906583204999E-4	0.0	0.0	30.260217541762756	0.0
34	2.553906583204999E-4	0.0	0.0	30.805731987935346	0.0
35	2.553906583204999E-4	0.0	0.0	31.39517362733906	0.0
36	2.553906583204999E-4	0.0	0.0	31.96239627946889	0.0
37	2.553906583204999E-4	0.0	0.0	32.50510142839995	0.0
38	2.553906583204999E-4	0.0	0.0	33.026353762032095	0.0
39	2.553906583204999E-4	0.0	0.0	33.55169234619736	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATGCG	20	7.027582E-4	45.000004	1
CATTGCG	20	7.027582E-4	45.000004	1
ATATGCG	20	7.027582E-4	45.000004	1
GTATGCG	20	7.027582E-4	45.000004	1
TAGGACG	20	7.027582E-4	45.000004	1
CGTCAGG	20	7.027582E-4	45.000004	2
TAATGCG	20	7.027582E-4	45.000004	1
AACGTAG	25	3.8860795E-5	45.0	1
CGCGAGG	35	1.2091914E-7	45.0	2
CGAATAT	35	1.2091914E-7	45.0	14
TTAAACG	25	3.8860795E-5	45.0	1
GGACCTA	25	3.8860795E-5	45.0	8
TATACGG	25	3.8860795E-5	45.0	2
CCGTTAG	25	3.8860795E-5	45.0	31
CGTTTTT	1585	0.0	42.72871	1
GCGGGTA	55	6.002665E-11	40.909092	5
GCAACAG	50	1.0768417E-9	40.5	1
TAAACGG	45	1.9226718E-8	40.0	2
CGACAGG	40	3.450441E-7	39.375004	2
CTACGAA	40	3.450441E-7	39.375004	11
>>END_MODULE