FastQCFastQC Report
Sat 18 Jun 2016
SRR3551999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434859
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140423.229092648421672No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC21650.49786252555426014No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG14890.3424098385913595No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC10950.2518057577283671No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC9350.21501222235253267No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC7540.17338953545861993No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT6980.16051179807707786No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5780.13291664654520202No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT4910.11291016168459203No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG4670.10739113137821685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT406.7975634E-945.0000044
TTATGAG207.028443E-445.0000041
GTAATCG207.028443E-445.0000041
CGAATGC207.028443E-445.00000445
TGTAGCG207.028443E-445.0000041
TAAGCGG406.7975634E-945.0000042
TATCGAG207.028443E-445.0000041
ACCGGTA253.8867925E-545.041
ACGGGTA351.2095916E-745.05
GTATGCG351.2095916E-745.01
CGAATAT302.1623782E-644.99999614
ACGTAGG302.1623782E-644.9999962
CGTTTTT63150.043.6460841
TAAGGGC1000.040.54
TACCGGT403.4516052E-739.37500440
GTTTTTT71900.038.8351862
ATGTGCG356.2393356E-638.5714261
ATTCGGC356.2393356E-638.57142617
GCGATAT356.2393356E-638.5714269
AAGGGAT1950.038.0769275