##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551999_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434859
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31620824221184	33.0	31.0	34.0	30.0	34.0
2	32.45101055744506	34.0	31.0	34.0	31.0	34.0
3	32.36906905456711	34.0	31.0	34.0	30.0	34.0
4	35.964664408463435	37.0	35.0	37.0	35.0	37.0
5	36.01914413637524	37.0	35.0	37.0	35.0	37.0
6	35.99346684787483	37.0	35.0	37.0	35.0	37.0
7	36.15434198211374	37.0	36.0	37.0	35.0	37.0
8	36.00695167859007	37.0	36.0	37.0	35.0	37.0
9	37.90411604681058	39.0	38.0	39.0	35.0	39.0
10	37.66918242464799	39.0	37.0	39.0	35.0	39.0
11	37.618793677950784	39.0	37.0	39.0	35.0	39.0
12	37.51876125364773	39.0	37.0	39.0	35.0	39.0
13	37.47654757059185	39.0	37.0	39.0	35.0	39.0
14	38.70490204871004	40.0	38.0	41.0	35.0	41.0
15	38.78711030471946	40.0	38.0	41.0	35.0	41.0
16	38.811642394431296	40.0	38.0	41.0	35.0	41.0
17	38.75593698187229	40.0	38.0	41.0	35.0	41.0
18	38.4830876215049	40.0	38.0	41.0	35.0	41.0
19	38.190988343348074	40.0	37.0	41.0	35.0	41.0
20	37.87131231042706	40.0	35.0	41.0	34.0	41.0
21	37.7773048275418	40.0	35.0	41.0	34.0	41.0
22	37.805458780892195	40.0	35.0	41.0	34.0	41.0
23	37.757215557226594	40.0	35.0	41.0	34.0	41.0
24	37.67622838667246	39.0	35.0	41.0	34.0	41.0
25	37.50441177485116	39.0	35.0	41.0	33.0	41.0
26	37.49590556939146	39.0	35.0	41.0	33.0	41.0
27	37.49087865262074	39.0	35.0	41.0	33.0	41.0
28	37.3678617666876	39.0	35.0	41.0	33.0	41.0
29	37.249690129444254	39.0	35.0	41.0	33.0	41.0
30	36.98006480261418	39.0	35.0	41.0	33.0	41.0
31	36.735396990748725	39.0	35.0	41.0	32.0	41.0
32	36.29777698058451	39.0	35.0	41.0	31.0	41.0
33	35.7728482105694	39.0	35.0	41.0	27.0	41.0
34	35.30671091089295	39.0	35.0	41.0	23.0	41.0
35	34.990806215347966	39.0	35.0	41.0	21.0	41.0
36	34.82727044858218	38.0	35.0	41.0	21.0	41.0
37	34.741608199439355	38.0	35.0	41.0	20.0	41.0
38	34.58183687126172	38.0	35.0	41.0	18.0	41.0
39	34.49551233848213	38.0	35.0	41.0	18.0	41.0
40	34.38277234689865	38.0	34.0	41.0	18.0	41.0
41	34.2631749601595	38.0	34.0	40.0	18.0	41.0
42	34.149096603726726	38.0	34.0	40.0	18.0	41.0
43	34.03313027900998	38.0	34.0	40.0	17.0	41.0
44	34.04624027558358	37.0	34.0	40.0	18.0	41.0
45	34.02656033334943	37.0	34.0	40.0	18.0	41.0
46	33.970036264628305	37.0	34.0	40.0	18.0	41.0
47	33.91979699166856	37.0	34.0	40.0	18.0	41.0
48	33.88303795023214	37.0	34.0	40.0	18.0	41.0
49	33.83632855707252	36.0	34.0	40.0	18.0	41.0
50	33.731103645089554	36.0	34.0	40.0	18.0	41.0
51	32.99526743151228	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	6.0
11	15.0
12	13.0
13	17.0
14	15.0
15	31.0
16	58.0
17	101.0
18	182.0
19	311.0
20	539.0
21	906.0
22	1366.0
23	1991.0
24	3234.0
25	5761.0
26	9469.0
27	11862.0
28	11371.0
29	9707.0
30	8424.0
31	8640.0
32	10048.0
33	13498.0
34	24853.0
35	31568.0
36	34099.0
37	44107.0
38	77301.0
39	125208.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.75499644712424	16.646545202008006	20.768111042889764	24.830347307977988
2	42.14423525786519	21.98735682140648	20.67796688121897	15.19044103950936
3	27.803724885537612	22.165345548787077	35.09827323339289	14.932656332282418
4	24.520361772436583	21.52444815445926	36.87678074962229	17.078409323481864
5	23.37148363032615	24.840695489802442	33.896044464987504	17.891776414883907
6	23.256043913084472	29.783906967545803	33.426926888945616	13.533122230424114
7	67.81232537443171	5.409799498228161	22.2943528821986	4.483522245141529
8	68.39205351619721	5.887655538921812	21.056250416801767	4.664040528079216
9	62.9252240381365	6.43725897359834	23.023554761428418	7.613962226836744
10	35.95786220361082	21.165481224948778	28.159702340298807	14.716954231141589
11	27.9173249260105	20.193671971834547	33.8516622629404	18.03734083921455
12	24.189449913650172	18.188194334255474	38.534329518303636	19.08802623379072
13	22.761630781471695	18.080573243281155	40.84979269142412	18.308003283823034
14	18.553600132456726	21.31357520483651	39.8092255190763	20.323599143630464
15	18.05044853619219	20.047187709119505	41.96302709613921	19.93933665854909
16	21.392221386702357	20.21965740619373	38.466491437454444	19.921629769649474
17	21.530197144361736	20.728098073168542	37.28035984077598	20.461344941693742
18	22.19616013466434	20.01982251718373	37.027864204259316	20.756153143892618
19	22.323787710499264	22.143729346753776	35.36042717294571	20.172055769801243
20	23.388040721245275	22.596059872280442	35.164041677877194	18.851857728597086
21	22.519023407587287	23.029533710926987	35.824945557065625	18.6264973244201
22	21.73210166973663	20.30428253755815	36.20483880982112	21.758776982884108
23	21.28758977047733	22.89661706438179	36.119293840072295	19.696499325068586
24	21.475696720086283	21.976778679985927	35.723993294378175	20.823531305549615
25	22.22513504377281	22.770139286527357	33.978829919583134	21.025895750116703
26	20.75132399237454	21.825695225348905	35.33559153656702	22.08738924570953
27	22.227434639733797	21.15214356837504	35.47862640534058	21.141795386550584
28	20.85595560859957	22.474641205540184	35.199455455676436	21.469947730183804
29	21.917449104192393	23.464387307150133	33.988258263023184	20.629905325634287
30	22.41577154893885	23.4273638121782	34.109906889359536	20.046957749523408
31	23.241096539338038	22.72966639761394	32.566188120747185	21.463048942300837
32	23.783801186131598	23.786560701284785	32.614249676331866	19.81538843625175
33	23.72056229720438	24.234981913677768	30.661662745855555	21.3827930432623
34	21.88456488195024	25.165628399090277	31.505844423134853	21.443962295824623
35	22.003453993133405	25.9175962783339	30.412616503280375	21.666333225252323
36	23.737579307315706	27.164207248786386	28.132337148363035	20.965876295534873
37	23.393559751551653	25.90563837933675	29.235913250041968	21.46488861906963
38	23.133015529171523	25.913916924796315	28.846361694250323	22.10670585178184
39	23.46875653947601	24.122761630781472	28.82612524979361	23.582356579948904
40	23.338599408083997	24.134949489374716	30.250035068838404	22.27641603370288
41	21.73371138690932	25.03386155052557	29.359401553147112	23.873025509417996
42	22.21639657912105	25.00994575253128	30.117118422293203	22.656539246054468
43	23.066327246302826	23.956960761994118	30.7175429277076	22.259169063995458
44	23.729990640644438	23.823584196256718	30.2739508668327	22.172474296266145
45	22.731276114786635	22.90581544822575	30.535184968001122	23.827723468986502
46	22.651020215748094	24.42952773197749	30.308214846651442	22.611237205622974
47	21.46902789179941	24.693751307895205	32.364053635776195	21.47316716452919
48	21.613212558553464	24.240041024791946	31.380746402857017	22.766000013797576
49	22.346323750916962	23.41862534752644	31.97243244361966	22.26261845793694
50	20.418572456819337	23.763564741674887	32.696345252139196	23.121517549366576
51	21.021296558194724	23.25167468075859	31.670495493941715	24.05653326710497
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	504.0
2	752.0
3	10955.5
4	21159.0
5	13577.5
6	5996.0
7	5880.5
8	5765.0
9	5740.0
10	5715.0
11	5503.0
12	5291.0
13	5084.0
14	4877.0
15	4492.5
16	4108.0
17	3744.5
18	3381.0
19	3213.5
20	3046.0
21	2998.0
22	2950.0
23	2827.5
24	2705.0
25	2781.5
26	3040.0
27	3222.0
28	3671.0
29	4120.0
30	4440.0
31	4760.0
32	5258.0
33	5756.0
34	6640.5
35	7525.0
36	7966.5
37	8408.0
38	9211.0
39	10014.0
40	10874.5
41	11735.0
42	13302.5
43	14870.0
44	16797.5
45	18725.0
46	25114.5
47	31504.0
48	31171.0
49	30838.0
50	31308.0
51	31778.0
52	29296.0
53	26814.0
54	25458.0
55	24102.0
56	23058.5
57	22015.0
58	21715.5
59	21416.0
60	20697.5
61	19979.0
62	18893.0
63	17807.0
64	16220.5
65	14634.0
66	12671.5
67	10709.0
68	9457.0
69	8205.0
70	7275.0
71	6345.0
72	5496.0
73	4647.0
74	3822.5
75	2315.0
76	1632.0
77	1197.5
78	763.0
79	565.0
80	367.0
81	270.5
82	174.0
83	119.0
84	64.0
85	54.0
86	44.0
87	31.0
88	18.0
89	13.0
90	8.0
91	6.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434859.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.776759822848746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.03589932302107	30.659447436411085
2	9.378247820628344	8.210986048056036
3	5.254516024287912	6.900770579416861
4	3.8159141842921755	6.681934350014415
5	2.913154538408374	6.37642332773726
6	2.2754144014260484	5.976616184920756
7	1.7382816325660178	5.3267436273318385
8	1.2639755807710658	4.426620433708856
9	0.9593005110797269	3.7795561264325936
>10	2.30354788452452	13.342880470009163
>50	0.03536002250129969	1.1011218528588786
>100	0.02216598425453953	2.1904608239514802
>500	0.0021110461194799555	0.6850244006978314
>1k	0.0015832845896099667	1.097194225603373
>5k	0.0	0.0
>10k+	5.277615298699889E-4	3.2442201128495607
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14042	3.229092648421672	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTC	2165	0.49786252555426014	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	1489	0.3424098385913595	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGC	1095	0.2518057577283671	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCC	935	0.21501222235253267	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCTGC	754	0.17338953545861993	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTTCT	698	0.16051179807707786	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	578	0.13291664654520202	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCGTCTT	491	0.11291016168459203	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTACCGTCGTATGCCG	467	0.10739113137821685	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08485509096051824	0.0
2	0.0	0.0	0.0	0.3748341416413136	0.0
3	0.0	0.0	0.0	0.6342285660409466	0.0
4	0.0	0.0	0.0	1.063793091553814	0.0
5	0.0	0.0	0.0	2.2432558599454078	0.0
6	0.0	0.0	0.0	3.271635173699981	0.0
7	0.0	0.0	0.0	4.1247852752271426	0.0
8	0.0	0.0	0.0	5.257106326418448	0.0
9	0.0	0.0	0.0	6.0509268521520765	0.0
10	0.0	0.0	0.0	7.446551640876698	0.0
11	0.0	0.0	0.0	8.474011116246876	0.0
12	0.0	0.0	0.0	9.399368530949113	0.0
13	0.0	0.0	0.0	9.75304638974932	0.0
14	0.0	0.0	0.0	9.95311123835542	0.0
15	0.0	0.0	0.0	10.173872450610428	0.0
16	0.0	0.0	0.0	10.444074976026712	0.0
17	0.0	0.0	0.0	10.745781966108554	0.0
18	0.0	0.0	0.0	11.119236350173274	0.0
19	0.0	0.0	0.0	11.381850208918292	0.0
20	0.0	0.0	0.0	11.607670532287477	0.0
21	0.0	0.0	0.0	11.839469805155234	0.0
22	0.0	0.0	0.0	12.0912755628836	0.0
23	0.0	0.0	0.0	12.328593866057734	0.0
24	0.0	0.0	0.0	12.53394778537411	0.0
25	0.0	0.0	0.0	12.715155947100094	0.0
26	0.0	0.0	0.0	12.902343058324652	0.0
27	0.0	0.0	0.0	13.125633826136747	0.0
28	0.0	0.0	0.0	13.319719725244274	0.0
29	0.0	0.0	0.0	13.537491462749994	0.0
30	0.0	0.0	0.0	13.78331827097979	0.0
31	0.0	0.0	0.0	13.989132109488363	0.0
32	0.0	0.0	0.0	14.2032244934565	0.0
33	0.0	0.0	0.0	14.40995817034947	0.0
34	0.0	0.0	0.0	14.626350150278595	0.0
35	2.2995959609896542E-4	0.0	0.0	14.851250635263384	0.0
36	2.2995959609896542E-4	0.0	0.0	15.05913411013685	0.0
37	2.2995959609896542E-4	0.0	0.0	15.273686413297185	0.0
38	2.2995959609896542E-4	0.0	0.0	15.5369901508305	0.0
39	2.2995959609896542E-4	0.0	0.0	15.922402433892366	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGGT	40	6.7975634E-9	45.000004	4
TTATGAG	20	7.028443E-4	45.000004	1
GTAATCG	20	7.028443E-4	45.000004	1
CGAATGC	20	7.028443E-4	45.000004	45
TGTAGCG	20	7.028443E-4	45.000004	1
TAAGCGG	40	6.7975634E-9	45.000004	2
TATCGAG	20	7.028443E-4	45.000004	1
ACCGGTA	25	3.8867925E-5	45.0	41
ACGGGTA	35	1.2095916E-7	45.0	5
GTATGCG	35	1.2095916E-7	45.0	1
CGAATAT	30	2.1623782E-6	44.999996	14
ACGTAGG	30	2.1623782E-6	44.999996	2
CGTTTTT	6315	0.0	43.646084	1
TAAGGGC	100	0.0	40.5	4
TACCGGT	40	3.4516052E-7	39.375004	40
GTTTTTT	7190	0.0	38.835186	2
ATGTGCG	35	6.2393356E-6	38.571426	1
ATTCGGC	35	6.2393356E-6	38.571426	17
GCGATAT	35	6.2393356E-6	38.571426	9
AAGGGAT	195	0.0	38.076927	5
>>END_MODULE