##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551996_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480297
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27583141264676	33.0	31.0	34.0	30.0	34.0
2	32.358923749263475	34.0	31.0	34.0	30.0	34.0
3	32.35464931073898	34.0	31.0	34.0	30.0	34.0
4	35.918700304186785	37.0	35.0	37.0	35.0	37.0
5	35.974809336722906	37.0	35.0	37.0	35.0	37.0
6	35.95438863869647	37.0	35.0	37.0	35.0	37.0
7	36.122534598383915	37.0	35.0	37.0	35.0	37.0
8	35.944829969789524	37.0	35.0	37.0	35.0	37.0
9	37.832990836919656	39.0	38.0	39.0	35.0	39.0
10	37.539528666637516	39.0	37.0	39.0	35.0	39.0
11	37.503126190669526	39.0	37.0	39.0	35.0	39.0
12	37.429784071106006	39.0	37.0	39.0	35.0	39.0
13	37.41663595650191	39.0	37.0	39.0	35.0	39.0
14	38.673658174004835	40.0	38.0	41.0	35.0	41.0
15	38.715900786388424	40.0	38.0	41.0	35.0	41.0
16	38.72465578589914	40.0	38.0	41.0	35.0	41.0
17	38.67622533557361	40.0	38.0	41.0	35.0	41.0
18	38.57250409642367	40.0	38.0	41.0	35.0	41.0
19	38.50242245943656	40.0	37.0	41.0	34.0	41.0
20	38.4190011596991	40.0	37.0	41.0	34.0	41.0
21	38.31377460196504	40.0	37.0	41.0	34.0	41.0
22	38.32648132301472	40.0	37.0	41.0	34.0	41.0
23	38.29446155191475	40.0	37.0	41.0	34.0	41.0
24	38.215710279264705	40.0	37.0	41.0	34.0	41.0
25	38.0615993853803	40.0	37.0	41.0	34.0	41.0
26	38.07544290303708	40.0	37.0	41.0	34.0	41.0
27	38.081097737441624	40.0	37.0	41.0	34.0	41.0
28	37.98637301503028	40.0	36.0	41.0	34.0	41.0
29	37.92521085911426	40.0	36.0	41.0	34.0	41.0
30	37.792965602533435	40.0	36.0	41.0	33.0	41.0
31	37.77298213397127	40.0	36.0	41.0	33.0	41.0
32	37.65521750083802	40.0	36.0	41.0	33.0	41.0
33	37.54609960087196	40.0	36.0	41.0	33.0	41.0
34	37.43765628350791	40.0	36.0	41.0	33.0	41.0
35	37.33629400141996	40.0	35.0	41.0	33.0	41.0
36	37.2541198466782	40.0	35.0	41.0	33.0	41.0
37	37.21920603293379	40.0	35.0	41.0	33.0	41.0
38	37.08662556709702	40.0	35.0	41.0	32.0	41.0
39	37.02219668246939	40.0	35.0	41.0	32.0	41.0
40	36.915795851317	39.0	35.0	41.0	32.0	41.0
41	36.82804597988328	39.0	35.0	41.0	31.0	41.0
42	36.737805982548295	39.0	35.0	41.0	31.0	41.0
43	36.62246484987414	39.0	35.0	41.0	31.0	41.0
44	36.6612929083463	39.0	35.0	41.0	31.0	41.0
45	36.60343703999817	39.0	35.0	41.0	31.0	41.0
46	36.50958261242523	39.0	35.0	41.0	31.0	41.0
47	36.43866815741094	39.0	35.0	41.0	31.0	41.0
48	36.3770063106786	39.0	35.0	41.0	31.0	41.0
49	36.33559443427713	38.0	35.0	41.0	31.0	41.0
50	36.236241325679735	38.0	35.0	41.0	31.0	41.0
51	35.39929668517605	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	5.0
11	9.0
12	8.0
13	14.0
14	17.0
15	18.0
16	37.0
17	72.0
18	103.0
19	219.0
20	346.0
21	571.0
22	972.0
23	1393.0
24	1877.0
25	2659.0
26	3675.0
27	4466.0
28	4949.0
29	5046.0
30	6096.0
31	7434.0
32	9564.0
33	13447.0
34	25119.0
35	36461.0
36	35438.0
37	53049.0
38	91064.0
39	175952.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81287828156328	20.3405392913135	24.030339560730134	13.816242866393086
2	32.907138707924474	26.192126954780065	23.943310076889922	16.95742426040554
3	31.541317143350884	25.016187900403292	26.801957955181898	16.640537001063926
4	28.873592797789698	24.586453798378923	27.64289595812591	18.89705744570547
5	26.763023712411275	28.574194717018848	24.279768559870245	20.38301301069963
6	26.294979981136674	34.06725421978484	24.413227648725684	15.224538150352803
7	76.5796996441785	6.550530192776553	11.836842620295359	5.032927542749591
8	76.44665696433664	7.744999448258056	10.499961482166242	5.308382105239049
9	69.81992392207322	8.690456113613035	12.594707025028265	8.894912939285483
10	39.3475287166066	24.77133940041266	19.40278619270993	16.478345690270814
11	30.10449784196029	23.718032800538	24.802153667418285	21.375315690083426
12	26.75636116819385	21.303901544252827	30.552553940582598	21.38718334697073
13	24.693887323885015	22.803806811202236	32.00040808083332	20.501897784079436
14	21.265383710495797	26.487985558935407	29.86110677351722	22.385523957051575
15	20.619325125911676	23.853573934461387	33.17717995323727	22.34992098638967
16	21.975985692186292	24.06656714491242	30.466149070262777	23.491298092638512
17	22.657022633911932	24.288096740142038	28.621040731047664	24.433839894898366
18	23.147552451920376	23.69304825972263	29.656858152351568	23.50254113600543
19	24.50546224523576	24.750310745226393	27.536711659660583	23.207515349877266
20	25.325163388486704	24.700966277116034	28.585229555878968	21.38864077851829
21	24.943940936545513	25.84067774731052	27.89773827444269	21.31764304170128
22	22.98307089155252	24.254575814548083	27.147369231954393	25.614984061945005
23	23.086756735936305	25.24958515252021	27.822784651996578	23.840873459546906
24	22.841075417918496	24.682852485024892	28.655810883682385	23.820261213374224
25	22.74655057183368	27.020989096329977	27.015992188166905	23.216468143669438
26	22.107987349494167	26.02827000793259	27.346620944956975	24.51712169761627
27	24.875233449303245	24.753433812828312	27.11176626129249	23.25956647657595
28	21.85418605571136	26.0199418276608	28.562327060131544	23.563545056496295
29	23.389069679802287	25.974345040672752	27.13612618858748	23.500459090937483
30	22.59643512243466	26.604371878233675	26.476950720075287	24.322242279256375
31	24.20377391489016	25.605406654632446	26.101141585310756	24.089677845166637
32	25.005361266049963	26.938956520652845	25.23501083704458	22.820671376252612
33	23.32286064664156	26.297270230711412	25.48069215506239	24.89917696758464
34	22.31494262924815	26.35973158274984	27.400129503203225	23.92519628479878
35	24.591034297528406	25.10280097522991	25.755938513045052	24.550226214196634
36	23.325775509736683	27.723262897748686	25.36826172139322	23.58269987112141
37	23.847327799257542	26.183382365494683	27.30664567965238	22.662644155595395
38	23.3449303243618	27.525052207280076	25.85212899518423	23.277888473173892
39	24.352848341755205	24.818393618948274	27.20108599470744	23.627672044589076
40	23.689716987613917	25.266033308556995	28.716814804173254	22.327434899655838
41	21.579147902235494	25.36992735744758	26.851302423292257	26.199622317024673
42	21.947878083769	25.02451608067508	28.394930636668565	24.632675198887355
43	23.446325919170846	23.671811400029565	27.297068272339825	25.584794408459764
44	23.115697162380776	24.248329679344238	27.413454591638093	25.222518566636893
45	23.267894656847744	23.963089505035427	26.794254388430495	25.97476144968634
46	23.9085399242552	25.643091670362296	27.241894078039213	23.20647432734329
47	22.259560230440748	24.624347018615563	29.792607490781748	23.32348526016194
48	22.03324193155485	24.772796831960225	27.962698080562653	25.231263155922274
49	22.563538810361088	24.273522424666403	29.394520473790177	23.768418291182332
50	21.76215966370808	24.08280709644241	29.155918109003387	24.99911513084612
51	21.839819944742526	23.73198250249325	27.310393360774686	27.117804191989542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	568.0
2	905.0
3	2867.5
4	4830.0
5	3168.5
6	1507.0
7	1477.5
8	1448.0
9	1461.0
10	1474.0
11	1457.5
12	1441.0
13	1459.5
14	1478.0
15	1438.5
16	1399.0
17	1378.0
18	1357.0
19	1353.5
20	1350.0
21	1555.5
22	1761.0
23	1863.5
24	1966.0
25	2444.0
26	3335.5
27	3749.0
28	4887.0
29	6025.0
30	6874.5
31	7724.0
32	8056.0
33	8388.0
34	9528.0
35	10668.0
36	11399.0
37	12130.0
38	12803.5
39	13477.0
40	15253.0
41	17029.0
42	19451.5
43	21874.0
44	24485.5
45	27097.0
46	34916.5
47	42736.0
48	42838.5
49	42941.0
50	41246.5
51	39552.0
52	36150.5
53	32749.0
54	29972.0
55	27195.0
56	26473.5
57	25752.0
58	24568.5
59	23385.0
60	22434.5
61	21484.0
62	19975.5
63	18467.0
64	16665.5
65	14864.0
66	13200.5
67	11537.0
68	10183.0
69	8829.0
70	7530.5
71	6232.0
72	5581.5
73	4931.0
74	4114.0
75	2631.0
76	1965.0
77	1525.5
78	1086.0
79	854.5
80	623.0
81	468.5
82	314.0
83	200.5
84	87.0
85	54.0
86	21.0
87	15.5
88	10.0
89	8.5
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480297.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.23178317394606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.6387332276307	23.09044116249158
2	8.179295976591554	5.272665888660567
3	3.236745285627036	3.129782167068682
4	2.0300273740250594	2.6172560862700327
5	1.5746526423762255	2.537693127167586
6	1.3258452716100242	2.5640614390041554
7	1.2004806755730189	2.7085542987707276
8	1.0800284682843573	2.784899472914438
9	1.0165410783709266	2.948843846291491
>10	8.586646260923047	44.570787834483696
>50	0.07847253672057146	1.7316365528498296
>100	0.044748801931565545	2.8829950913694895
>500	0.004539733529289258	1.0123510170752927
>1k	0.0032426668066351844	2.1480320155824297
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3027	0.6302350420677205	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	2197	0.4574253014280747	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	2158	0.44930532566307935	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	1597	0.3325025973512223	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1297	0.270041245312796	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC	871	0.1813461254182308	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	805	0.16760462796977704	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	780	0.16239951529990818	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	714	0.1486580178514544	Illumina Single End Adapter 1 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	634	0.1320016573078741	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	536	0.1115976156419882	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	503	0.10472686691776129	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	487	0.10139559480904523	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15365492601452851	0.0
2	0.0	0.0	0.0	0.7489116109407304	0.0
3	0.0	0.0	0.0	1.0535148043814557	0.0
4	0.0	0.0	0.0	1.5598681649062975	0.0
5	2.0820450679475409E-4	0.0	0.0	3.1907340666296062	0.0
6	2.0820450679475409E-4	0.0	0.0	4.0560319968686045	0.0
7	4.1640901358950817E-4	0.0	0.0	4.903632544030048	0.0
8	4.1640901358950817E-4	0.0	0.0	6.003576953426734	0.0
9	4.1640901358950817E-4	0.0	0.0	6.5028513607205545	0.0
10	4.1640901358950817E-4	0.0	0.0	7.8632596081174775	0.0
11	4.1640901358950817E-4	0.0	0.0	9.067930884431924	0.0
12	4.1640901358950817E-4	0.0	0.0	10.305914881833532	0.0
13	4.1640901358950817E-4	0.0	0.0	10.726487985558935	0.0
14	4.1640901358950817E-4	0.0	0.0	10.911373587592678	0.0
15	4.1640901358950817E-4	0.0	0.0	11.278646337578623	0.0
16	4.1640901358950817E-4	0.0	0.0	11.86057793406996	0.0
17	4.1640901358950817E-4	0.0	0.0	12.53974103523445	0.0
18	4.1640901358950817E-4	0.0	0.0	13.248885585377382	0.0
19	4.1640901358950817E-4	0.0	0.0	13.71609649862481	0.0
20	4.1640901358950817E-4	0.0	0.0	14.123344513915349	0.0
21	4.1640901358950817E-4	0.0	0.0	14.592012858710339	0.0
22	4.1640901358950817E-4	0.0	0.0	15.09690878768762	0.0
23	4.1640901358950817E-4	0.0	0.0	15.570990449659273	0.0
24	4.1640901358950817E-4	0.0	0.0	15.97594821537507	0.0
25	4.1640901358950817E-4	0.0	0.0	16.34863428253768	0.0
26	4.1640901358950817E-4	0.0	0.0	16.698626058459663	0.0
27	4.1640901358950817E-4	0.0	0.0	17.036125563973957	0.0
28	6.246135203842623E-4	0.0	0.0	17.37133481991351	0.0
29	6.246135203842623E-4	0.0	0.0	17.704045621771527	0.0
30	6.246135203842623E-4	0.0	0.0	18.102549047776687	0.0
31	6.246135203842623E-4	0.0	0.0	18.44775212004239	0.0
32	6.246135203842623E-4	0.0	0.0	18.800866963566293	0.0
33	6.246135203842623E-4	0.0	0.0	19.140448514148538	0.0
34	6.246135203842623E-4	0.0	0.0	19.488566449509367	0.0
35	6.246135203842623E-4	0.0	0.0	19.842930520074038	0.0
36	6.246135203842623E-4	0.0	0.0	20.174392094891285	0.0
37	6.246135203842623E-4	0.0	0.0	20.52271823475891	0.0
38	6.246135203842623E-4	0.0	0.0	20.853763400562567	0.0
39	6.246135203842623E-4	0.0	0.0	21.17439834102649	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	25	3.8874015E-5	45.000004	21
TTTCACG	20	7.029182E-4	45.0	16
TATTGCG	20	7.029182E-4	45.0	1
CTCGAAT	20	7.029182E-4	45.0	43
GCATATT	55	1.8189894E-12	45.0	27
CGAATAT	110	0.0	40.909092	14
AACGGGA	165	0.0	40.90909	4
TTAGTCA	50	1.0786607E-9	40.500004	32
AGGGCGC	95	0.0	40.263157	6
TGATTCA	90	0.0	40.0	44
CGACAGG	40	3.4526238E-7	39.375	2
CGTTTTT	1320	0.0	38.863636	1
TATGCGG	35	6.2406943E-6	38.57143	2
CGTAAGG	70	0.0	38.57143	2
CTACGAA	135	0.0	38.333332	11
CATATGC	60	1.546141E-10	37.500004	33
GACAACG	30	1.13893235E-4	37.500004	1
TCGGCGT	60	1.546141E-10	37.500004	4
TCTCGTA	30	1.13893235E-4	37.500004	28
TACTACG	30	1.13893235E-4	37.500004	1
>>END_MODULE