SRR3551994_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329024
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	5093	1.5479113985605915	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	4861	1.4773998249367826	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	4389	1.3339452441159307	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3015	0.9163465279128573	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1409	0.4282362380859755	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	1285	0.39054901770083644	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	943	0.2866052324450496	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	874	0.26563411787589963	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	845	0.25682017117292355	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	756	0.22977047267068665	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	742	0.22551546391752575	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	489	0.14862137716397586	RNA PCR Primer, Index 38 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	425	0.12916990857809763	RNA PCR Primer, Index 38 (96% over 25bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	362	0.11002236918887374	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	353	0.10728700641898464	RNA PCR Primer, Index 38 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGA	30	2.1607611E-6	45.000004	15
CGACAAA	30	2.1607611E-6	45.000004	12
CCCTTAG	30	2.1607611E-6	45.000004	31
CAGCCCA	20	7.025938E-4	45.0	15
AGGCGCC	20	7.025938E-4	45.0	25
TGTGACG	20	7.025938E-4	45.0	1
CAGGCGC	20	7.025938E-4	45.0	24
CGTAAGC	20	7.025938E-4	45.0	43
CCCATTT	20	7.025938E-4	45.0	42
GGCTATC	20	7.025938E-4	45.0	32
GTACGAG	20	7.025938E-4	45.0	1
TCGGCGT	25	3.8847174E-5	44.999996	4
TGCGAAG	25	3.8847174E-5	44.999996	1
CGTTTTT	1520	0.0	42.48355	1
ATAGGGC	85	0.0	42.35294	4
GCGAACC	65	0.0	41.538464	33
ATGAATG	580	0.0	40.73276	21
GATGAAT	570	0.0	40.657894	20
ACGGGAC	50	1.0768417E-9	40.499996	5
GCATATT	50	1.0768417E-9	40.499996	27