##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551994_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329024
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17140390974519	33.0	31.0	34.0	30.0	34.0
2	32.248456039680995	34.0	31.0	34.0	30.0	34.0
3	32.24887546197238	34.0	31.0	34.0	30.0	34.0
4	35.85636002236919	37.0	35.0	37.0	35.0	37.0
5	35.894044203462364	37.0	35.0	37.0	35.0	37.0
6	35.86382452343902	37.0	35.0	37.0	35.0	37.0
7	36.034520278156	37.0	35.0	37.0	35.0	37.0
8	35.786960829605135	37.0	35.0	37.0	35.0	37.0
9	37.681980645788755	39.0	38.0	39.0	35.0	39.0
10	37.407976317837	39.0	37.0	39.0	34.0	39.0
11	37.38094789437852	39.0	37.0	39.0	35.0	39.0
12	37.27027207741685	39.0	37.0	39.0	34.0	39.0
13	37.19593403520716	39.0	37.0	39.0	34.0	39.0
14	38.44354211242949	40.0	38.0	41.0	34.0	41.0
15	38.43473728360241	40.0	38.0	41.0	34.0	41.0
16	38.52854806944174	40.0	38.0	41.0	34.0	41.0
17	38.3947949085781	40.0	38.0	41.0	34.0	41.0
18	38.30625729430072	40.0	37.0	41.0	34.0	41.0
19	38.207182454775335	40.0	37.0	41.0	34.0	41.0
20	37.97594400408481	40.0	36.0	41.0	33.0	41.0
21	37.98087069636257	40.0	36.0	41.0	34.0	41.0
22	37.96082960513519	40.0	36.0	41.0	34.0	41.0
23	37.85925646761331	40.0	36.0	41.0	33.0	41.0
24	37.838969193736624	40.0	36.0	41.0	33.0	41.0
25	37.759947602606495	40.0	36.0	41.0	33.0	41.0
26	37.719692180509625	40.0	35.0	41.0	33.0	41.0
27	37.64394390682747	40.0	35.0	41.0	33.0	41.0
28	37.56156997665824	40.0	35.0	41.0	33.0	41.0
29	37.49131066426765	40.0	35.0	41.0	33.0	41.0
30	37.32605524217078	40.0	35.0	41.0	33.0	41.0
31	37.308345287881735	40.0	35.0	41.0	33.0	41.0
32	37.042981666990855	40.0	35.0	41.0	32.0	41.0
33	36.85470664753939	40.0	35.0	41.0	31.0	41.0
34	36.70445924917331	40.0	35.0	41.0	31.0	41.0
35	36.51893782824353	40.0	35.0	41.0	30.0	41.0
36	36.44389163100564	39.0	35.0	41.0	30.0	41.0
37	36.367793838747325	39.0	35.0	41.0	30.0	41.0
38	36.294331112624	39.0	35.0	41.0	30.0	41.0
39	36.239481010503795	39.0	35.0	41.0	30.0	41.0
40	36.13285353044155	39.0	35.0	41.0	29.0	41.0
41	36.029162006418986	39.0	35.0	41.0	29.0	41.0
42	35.90535644816184	39.0	35.0	41.0	28.0	41.0
43	35.769579118848476	39.0	35.0	41.0	28.0	41.0
44	35.851430290799456	39.0	35.0	41.0	28.0	41.0
45	35.84746097549115	39.0	35.0	41.0	28.0	41.0
46	35.771302397393505	39.0	35.0	41.0	28.0	41.0
47	35.62482979964987	38.0	35.0	41.0	28.0	41.0
48	35.59568602898269	38.0	35.0	41.0	28.0	41.0
49	35.57939238475005	38.0	35.0	40.0	28.0	41.0
50	35.44996717564676	38.0	35.0	40.0	27.0	41.0
51	34.62109147053103	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	4.0
11	3.0
12	6.0
13	11.0
14	10.0
15	23.0
16	22.0
17	56.0
18	125.0
19	266.0
20	645.0
21	1045.0
22	1575.0
23	1815.0
24	2288.0
25	2915.0
26	3767.0
27	4177.0
28	4306.0
29	4313.0
30	4757.0
31	5976.0
32	7404.0
33	10089.0
34	18221.0
35	24834.0
36	23802.0
37	34038.0
38	60434.0
39	111973.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.16684497179537	17.59172583155028	22.25187220385139	13.989556992802957
2	32.43046099980548	29.97197772806847	21.14040313168644	16.457158140439603
3	29.306980645788755	29.7713844582766	25.691134993191987	15.230499902742656
4	26.689542404201518	22.227557868119042	32.91583592686248	18.167063800816962
5	29.23373370939506	25.408480840303444	24.600637035596186	20.75714841470531
6	23.74446848862089	36.760844193736624	25.62123127796149	13.873456039680995
7	71.69294641120405	5.4324304609998055	18.151563411787592	4.723059716008559
8	70.04534623614083	11.306470044738377	13.24310688581988	5.405076833300914
9	64.3527523828049	7.500364715035985	14.053078194903716	14.093804707255398
10	38.25009725734293	23.48643260066135	21.632160571873175	16.631309570122543
11	32.02046051351877	22.322991635868508	26.67708130713869	18.979466543474032
12	26.363730305388057	20.10066134993192	31.046975296634894	22.488633048045127
13	25.303321338261036	21.88685323866952	34.2838212410037	18.526004182065748
14	20.699705796537636	28.55992268041237	29.41913051935421	21.32124100369578
15	18.44303151137911	23.151806555144912	36.35084370744991	22.054318226026066
16	20.616429196654348	23.3256540556312	31.127212604551644	24.93070414316281
17	20.883886889710173	24.685737210659404	31.20653812487843	23.223837774751992
18	21.410292258315504	23.21168060688582	30.78012546197238	24.597901672826296
19	21.574717953705505	24.665373954483563	28.67663149192764	25.08327659988329
20	25.35438144329897	24.660511087337095	30.68894670297607	19.296160766387864
21	23.174297315697334	28.32863256175841	28.762339525384167	19.734730597160084
22	21.678661738961292	22.45094582765999	30.45218585878234	25.41820657459638
23	23.01382269986384	26.948186150554363	29.182369675160473	20.85562147442132
24	22.847877358490564	24.672364326006612	28.460233903909742	24.019524411593075
25	20.527985800427935	29.881406827465472	27.175525189651818	22.415082182454775
26	20.821885333592686	24.40429877455748	30.781037249562342	23.992778642287494
27	24.944684886208908	25.63885917136744	27.07796391752577	22.338492024897878
28	20.51339719898852	26.540313168644232	29.99446848862089	22.951821143746354
29	24.451711729235555	23.47305971600856	28.8972840886987	23.177944466057188
30	21.993836315891848	28.86901867340984	28.371182649290024	20.765962361408285
31	26.056761816767164	24.534076541528886	25.09573769694612	24.31342394475783
32	25.895983271737016	25.70055679828827	26.056153958373855	22.347305971600857
33	24.005847597743628	24.547145496985024	26.572225734292935	24.87478117097841
34	25.046805096284768	25.97044592491733	26.017554950398754	22.965194028399143
35	23.264564287103674	25.526709297801986	27.779128574207352	23.429597840886988
36	27.00623662711535	27.59677105621474	25.193906827465472	20.203085489204433
37	22.225126434545807	27.29983223108345	27.534161641703946	22.940879692666797
38	23.231739933865008	24.800926376191402	27.369128087920636	24.59820560202295
39	24.34867973156973	25.57594582765999	26.59258899046878	23.482785450301495
40	21.874696070803346	23.80586218634507	31.857250534915387	22.4621912079362
41	23.016254133437076	26.75762254425209	26.79105475588407	23.435068566426764
42	23.34176230305388	24.20552907994554	30.52664851196265	21.92606010503793
43	26.474056603773583	23.256966057187316	26.576176813849443	23.692800525189654
44	23.51925695390002	24.057211631978216	27.808913635479477	24.614617778642288
45	22.825994456331454	22.721138883485704	27.841737988718148	26.611128671464694
46	26.22118751215717	25.643418109317253	25.980475588406925	22.154918790118654
47	21.11092199961097	24.8322310834468	32.842588990468776	21.214257926473447
48	23.098618945730404	24.856241489982494	27.797060396809957	24.248079167477144
49	22.780101147636646	22.506260941451078	31.583410328729823	23.130227582182457
50	22.579507877844776	22.34548239642093	30.154335246061077	24.920674479673217
51	22.442435810153665	22.67615736238086	27.30378331063995	27.577623516825522
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	431.5
2	692.0
3	2717.5
4	4743.0
5	3137.0
6	1531.0
7	1524.5
8	1518.0
9	1501.0
10	1484.0
11	1407.5
12	1331.0
13	1326.5
14	1322.0
15	1246.0
16	1170.0
17	1106.0
18	1042.0
19	1187.0
20	1332.0
21	1295.5
22	1259.0
23	1323.5
24	1388.0
25	1699.5
26	2348.0
27	2685.0
28	3059.0
29	3433.0
30	3735.5
31	4038.0
32	4573.0
33	5108.0
34	5765.5
35	6423.0
36	6958.0
37	7493.0
38	8332.0
39	9171.0
40	10353.5
41	11536.0
42	12698.0
43	13860.0
44	15724.5
45	17589.0
46	29768.0
47	41947.0
48	35202.0
49	28457.0
50	28440.0
51	28423.0
52	25690.0
53	22957.0
54	21150.0
55	19343.0
56	18011.0
57	16679.0
58	15560.5
59	14442.0
60	13682.5
61	12923.0
62	12236.0
63	11549.0
64	10158.5
65	8768.0
66	7395.5
67	6023.0
68	5411.0
69	4799.0
70	4091.5
71	3384.0
72	3026.0
73	2668.0
74	2307.0
75	1557.5
76	1169.0
77	907.5
78	646.0
79	478.5
80	311.0
81	232.0
82	153.0
83	106.0
84	59.0
85	44.0
86	29.0
87	22.5
88	16.0
89	9.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.766362134432928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.88976259396948	22.201435080495795
2	9.13607395502714	5.80439667484706
3	3.594773200443947	3.425786018293706
4	2.335915422040514	2.968141408477828
5	1.8339264198838603	2.91285853909674
6	1.5215098921607486	2.89997005353002
7	1.3229505989337844	2.9417729468186873
8	1.1812751365479928	3.0019851014388195
9	1.1503461779609905	3.288808194216269
>10	7.920726696775818	39.23813897761365
>50	0.06385269026871918	1.4106663365731649
>100	0.03791253484705202	2.232472180368765
>500	0.004988491427243686	1.3184389935170449
>1k	0.004988491427243686	4.740992525005161
>5k	9.976982854487371E-4	1.6141369697072856
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	5093	1.5479113985605915	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	4861	1.4773998249367826	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	4389	1.3339452441159307	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3015	0.9163465279128573	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1409	0.4282362380859755	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	1285	0.39054901770083644	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	943	0.2866052324450496	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	874	0.26563411787589963	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	845	0.25682017117292355	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	756	0.22977047267068665	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	742	0.22551546391752575	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	489	0.14862137716397586	RNA PCR Primer, Index 38 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	425	0.12916990857809763	RNA PCR Primer, Index 38 (96% over 25bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	362	0.11002236918887374	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	353	0.10728700641898464	RNA PCR Primer, Index 38 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.039291966543474E-4	0.0	0.0	0.15409210270375415	0.0
2	3.039291966543474E-4	0.0	0.0	0.8762278739544835	0.0
3	3.039291966543474E-4	0.0	0.0	1.174990274265707	0.0
4	3.039291966543474E-4	0.0	0.0	1.8500170200350126	0.0
5	3.039291966543474E-4	0.0	0.0	4.431591616417039	0.0
6	3.039291966543474E-4	0.0	0.0	5.218464306555145	0.0
7	3.039291966543474E-4	0.0	0.0	6.222950301497763	0.0
8	3.039291966543474E-4	0.0	0.0	7.4146566815794595	0.0
9	3.039291966543474E-4	0.0	0.0	7.831039680995915	0.0
10	3.039291966543474E-4	0.0	0.0	10.238158918498346	0.0
11	3.039291966543474E-4	0.0	0.0	11.4514442715425	0.0
12	3.039291966543474E-4	0.0	0.0	13.695961388834856	0.0
13	3.039291966543474E-4	0.0	0.0	14.1743459443688	0.0
14	3.039291966543474E-4	0.0	0.0	14.371292063800817	0.0
15	3.039291966543474E-4	0.0	0.0	14.930217856448161	0.0
16	3.039291966543474E-4	0.0	0.0	15.551449134409648	0.0
17	3.039291966543474E-4	0.0	0.0	16.21067156195293	0.0
18	3.039291966543474E-4	0.0	0.0	16.903326201128184	0.0
19	3.039291966543474E-4	0.0	0.0	17.572274362964404	0.0
20	3.039291966543474E-4	0.0	0.0	18.014187414899826	0.0
21	3.039291966543474E-4	0.0	0.0	18.469777280684692	0.0
22	3.039291966543474E-4	0.0	0.0	18.990711923750244	0.0
23	9.117875899630422E-4	0.0	0.0	19.455115736238085	0.0
24	9.117875899630422E-4	0.0	0.0	19.829860435712895	0.0
25	9.117875899630422E-4	0.0	0.0	20.19153617973157	0.0
26	9.117875899630422E-4	0.0	0.0	20.523730791674772	0.0
27	9.117875899630422E-4	0.0	0.0	20.889357615249953	0.0
28	9.117875899630422E-4	0.0	0.0	21.20453219218051	0.0
29	9.117875899630422E-4	0.0	0.0	21.538550379303636	0.0
30	9.117875899630422E-4	0.0	0.0	21.904177202878817	0.0
31	0.0012157167866173896	0.0	0.0	22.257039000194514	0.0
32	0.0012157167866173896	0.0	0.0	22.58953754133437	0.0
33	0.0012157167866173896	0.0	0.0	22.930242170783895	0.0
34	0.0012157167866173896	0.0	0.0	23.256662127990662	0.0
35	0.0012157167866173896	0.0	0.0	23.613171075666212	0.0
36	0.0012157167866173896	0.0	0.0	23.951140342345848	0.0
37	0.001519645983271737	0.0	0.0	24.290021396615444	0.0
38	0.001519645983271737	0.0	0.0	24.617960999805486	0.0
39	0.001519645983271737	0.0	0.0	24.94833203656876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACGA	30	2.1607611E-6	45.000004	15
CGACAAA	30	2.1607611E-6	45.000004	12
CCCTTAG	30	2.1607611E-6	45.000004	31
CAGCCCA	20	7.025938E-4	45.0	15
AGGCGCC	20	7.025938E-4	45.0	25
TGTGACG	20	7.025938E-4	45.0	1
CAGGCGC	20	7.025938E-4	45.0	24
CGTAAGC	20	7.025938E-4	45.0	43
CCCATTT	20	7.025938E-4	45.0	42
GGCTATC	20	7.025938E-4	45.0	32
GTACGAG	20	7.025938E-4	45.0	1
TCGGCGT	25	3.8847174E-5	44.999996	4
TGCGAAG	25	3.8847174E-5	44.999996	1
CGTTTTT	1520	0.0	42.48355	1
ATAGGGC	85	0.0	42.35294	4
GCGAACC	65	0.0	41.538464	33
ATGAATG	580	0.0	40.73276	21
GATGAAT	570	0.0	40.657894	20
ACGGGAC	50	1.0768417E-9	40.499996	5
GCATATT	50	1.0768417E-9	40.499996	27
>>END_MODULE