FastQCFastQC Report
Sat 18 Jun 2016
SRR3551993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398234
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG24970.6270182857315046No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC23690.5948763792142309No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21720.5454079762149892No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC16920.4248758267752125No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC14570.36586529527865524TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC7860.1973713947076342TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT7620.19134478723564538TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC6460.16221618445436603No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT5990.15041407815505456No Hit
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC4640.1165144111251174TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT4260.10697228262780176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.209246E-745.0000046
ATACCGG351.209246E-745.0000042
CGGCGAA207.0277276E-445.031
TCGTTCA253.8861985E-545.016
CAACGAT207.0277276E-445.037
GCGAAAT207.0277276E-445.017
CGTTCAT253.8861985E-545.017
TGATTCG253.8861985E-545.015
TGCGAAA207.0277276E-445.016
GTACTAG253.8861985E-545.01
GCGATAC253.8861985E-545.09
CTCGAAT207.0277276E-445.043
TCGAATG207.0277276E-445.044
ACACGAC501.0786607E-940.526
TTAATAG451.9228537E-840.0000041
TAGTTAA750.039.038
CGTTTTT10350.038.2608681
GCGAAAG301.13858245E-437.4999961
CGCATTG301.13858245E-437.49999624
GCCGATT301.13858245E-437.4999969