Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551993_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 398234 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG | 2497 | 0.6270182857315046 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC | 2369 | 0.5948763792142309 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2172 | 0.5454079762149892 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC | 1692 | 0.4248758267752125 | No Hit |
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC | 1457 | 0.36586529527865524 | TruSeq Adapter, Index 23 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC | 786 | 0.1973713947076342 | TruSeq Adapter, Index 23 (95% over 22bp) |
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT | 762 | 0.19134478723564538 | TruSeq Adapter, Index 20 (95% over 23bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC | 646 | 0.16221618445436603 | No Hit |
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT | 599 | 0.15041407815505456 | No Hit |
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC | 464 | 0.1165144111251174 | TruSeq Adapter, Index 23 (95% over 22bp) |
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT | 426 | 0.10697228262780176 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGGGTAT | 35 | 1.209246E-7 | 45.000004 | 6 |
ATACCGG | 35 | 1.209246E-7 | 45.000004 | 2 |
CGGCGAA | 20 | 7.0277276E-4 | 45.0 | 31 |
TCGTTCA | 25 | 3.8861985E-5 | 45.0 | 16 |
CAACGAT | 20 | 7.0277276E-4 | 45.0 | 37 |
GCGAAAT | 20 | 7.0277276E-4 | 45.0 | 17 |
CGTTCAT | 25 | 3.8861985E-5 | 45.0 | 17 |
TGATTCG | 25 | 3.8861985E-5 | 45.0 | 15 |
TGCGAAA | 20 | 7.0277276E-4 | 45.0 | 16 |
GTACTAG | 25 | 3.8861985E-5 | 45.0 | 1 |
GCGATAC | 25 | 3.8861985E-5 | 45.0 | 9 |
CTCGAAT | 20 | 7.0277276E-4 | 45.0 | 43 |
TCGAATG | 20 | 7.0277276E-4 | 45.0 | 44 |
ACACGAC | 50 | 1.0786607E-9 | 40.5 | 26 |
TTAATAG | 45 | 1.9228537E-8 | 40.000004 | 1 |
TAGTTAA | 75 | 0.0 | 39.0 | 38 |
CGTTTTT | 1035 | 0.0 | 38.260868 | 1 |
GCGAAAG | 30 | 1.13858245E-4 | 37.499996 | 1 |
CGCATTG | 30 | 1.13858245E-4 | 37.499996 | 24 |
GCCGATT | 30 | 1.13858245E-4 | 37.499996 | 9 |