##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551993_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398234
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29922608315714	33.0	31.0	34.0	30.0	34.0
2	32.381875982462574	34.0	31.0	34.0	30.0	34.0
3	32.39080038369401	34.0	31.0	34.0	30.0	34.0
4	35.954496602500036	37.0	35.0	37.0	35.0	37.0
5	36.005165304820785	37.0	35.0	37.0	35.0	37.0
6	35.98790158549998	37.0	35.0	37.0	35.0	37.0
7	36.137391583842664	37.0	36.0	37.0	35.0	37.0
8	35.96529678530713	37.0	35.0	37.0	35.0	37.0
9	37.843898311043255	39.0	38.0	39.0	35.0	39.0
10	37.55722012686009	39.0	37.0	39.0	35.0	39.0
11	37.526421651591775	39.0	37.0	39.0	35.0	39.0
12	37.4729957763526	39.0	37.0	39.0	35.0	39.0
13	37.43106565486624	39.0	37.0	39.0	35.0	39.0
14	38.70240361194674	40.0	38.0	41.0	35.0	41.0
15	38.7489239994576	40.0	38.0	41.0	35.0	41.0
16	38.77264623311922	40.0	38.0	41.0	35.0	41.0
17	38.71754546322012	40.0	38.0	41.0	35.0	41.0
18	38.64951008703426	40.0	38.0	41.0	35.0	41.0
19	38.56958471652345	40.0	38.0	41.0	35.0	41.0
20	38.46294389730661	40.0	38.0	41.0	34.0	41.0
21	38.39841148671384	40.0	37.0	41.0	34.0	41.0
22	38.414572839084556	40.0	37.0	41.0	34.0	41.0
23	38.35878403149907	40.0	37.0	41.0	34.0	41.0
24	38.285605950270444	40.0	37.0	41.0	34.0	41.0
25	38.133014760166134	40.0	37.0	41.0	34.0	41.0
26	38.15852488737777	40.0	37.0	41.0	34.0	41.0
27	38.14054550841967	40.0	37.0	41.0	34.0	41.0
28	38.06149148490586	40.0	37.0	41.0	34.0	41.0
29	38.027551640492774	40.0	37.0	41.0	34.0	41.0
30	37.88100965763847	40.0	36.0	41.0	33.0	41.0
31	37.84767749614548	40.0	36.0	41.0	33.0	41.0
32	37.712000984345885	40.0	36.0	41.0	33.0	41.0
33	37.623382232556736	40.0	36.0	41.0	33.0	41.0
34	37.53449981669069	40.0	36.0	41.0	33.0	41.0
35	37.44334486759041	40.0	36.0	41.0	33.0	41.0
36	37.338911293360184	40.0	35.0	41.0	33.0	41.0
37	37.27231476970826	40.0	35.0	41.0	33.0	41.0
38	37.181360707523716	40.0	35.0	41.0	32.0	41.0
39	37.14486206602148	40.0	35.0	41.0	33.0	41.0
40	37.07829065323403	40.0	35.0	41.0	32.0	41.0
41	36.99190676838241	39.0	35.0	41.0	32.0	41.0
42	36.88151438601425	39.0	35.0	41.0	32.0	41.0
43	36.76632582853297	39.0	35.0	41.0	31.0	41.0
44	36.788245102125885	39.0	35.0	41.0	32.0	41.0
45	36.74691764138672	39.0	35.0	41.0	32.0	41.0
46	36.68144106229001	39.0	35.0	41.0	31.0	41.0
47	36.589871783926036	39.0	35.0	41.0	31.0	41.0
48	36.56331704475258	39.0	35.0	41.0	31.0	41.0
49	36.516304484298175	39.0	35.0	41.0	31.0	41.0
50	36.43054837105822	39.0	35.0	41.0	31.0	41.0
51	35.599215536594066	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	6.0
11	12.0
12	6.0
13	16.0
14	10.0
15	15.0
16	35.0
17	49.0
18	88.0
19	171.0
20	293.0
21	492.0
22	836.0
23	1141.0
24	1645.0
25	2242.0
26	2867.0
27	3434.0
28	3566.0
29	4034.0
30	4786.0
31	5758.0
32	7573.0
33	10783.0
34	20239.0
35	28832.0
36	29106.0
37	42712.0
38	76179.0
39	151112.0
40	192.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53043687881999	20.02993215044421	22.9684055103281	13.471225460407698
2	32.37769753461532	26.075372770783005	24.42408232345807	17.1228473711436
3	31.347398765549904	25.919434302445293	25.785342286193547	16.947824645811256
4	28.309988599667534	24.299783544348298	28.19020977616176	19.200018079822414
5	26.740810679148442	28.110357227157902	24.30681458639895	20.842017507294706
6	25.981960354967182	34.928710255779265	23.899516364750372	15.189813024503183
7	76.00983341452513	6.655132409588332	12.331694430912478	5.003339744974061
8	76.42441378686902	7.7100398258310445	10.28114123856828	5.58440514873165
9	69.57391885173038	8.24565456490405	12.402758177353014	9.777668406012545
10	38.597407554352465	25.007156596372987	19.594509760593017	16.80092608868153
11	29.984632150946428	23.859087872959115	25.53624251068467	20.620037465409784
12	26.557250259897447	20.84528191967537	30.791444226258935	21.806023594168252
13	24.27065494156702	22.619615602886746	32.88594143141972	20.223788024126517
14	21.30255076161252	26.924622207044102	29.284541249617057	22.488285781726322
15	19.590743130923023	24.266888311897024	34.05058332538156	22.09178523179839
16	21.630498651546578	23.69662057986008	29.978856652119106	24.694024116474232
17	21.767603971534324	24.323136648302253	29.0100795009969	24.899179879166518
18	23.04901138526595	23.354108388535384	29.790274059974784	23.80660616622388
19	23.28831792363284	25.34866435311902	26.928639945358757	24.43437777788938
20	24.285972568891655	25.170879432695347	28.67861609003752	21.86453190837548
21	24.376120572326823	25.993511352621823	27.712852242651305	21.91751583240005
22	22.42500640327044	23.900771907973702	26.7782258672037	26.895995821552155
23	22.862186553634295	25.720556255869663	27.6453040172361	23.771953173259945
24	23.126855065112473	24.811542962178017	27.64856842961676	24.413033543092755
25	22.323056293535963	27.632999693647452	26.43144482891968	23.612499183896904
26	21.886629469106104	24.9817946232617	28.473711435989895	24.657864471642302
27	24.647317908566322	25.10534007643747	27.19607065192826	23.051271363067947
28	21.228724820080654	24.898928770521852	29.856817850811336	24.01552855858616
29	22.523189883334922	24.261615030359035	28.811703671710603	24.40349141459544
30	23.76617767443262	25.246714243384545	27.65685501489074	23.330253067292094
31	23.79505516856923	25.105842293726806	26.984637173119317	24.114465364584643
32	23.688585103230764	26.630071766850644	26.025402150494433	23.65594097942416
33	23.464345083543847	24.36758287840817	25.36649306689032	26.801578971157664
34	21.74148867248904	24.096134433524007	29.369164862869567	24.793212031117385
35	23.12208400086381	23.522602289106402	26.955508570338043	26.39980513969174
36	23.054535775448606	25.192223667491977	27.00196366960129	24.751276887458125
37	22.248225917425433	26.515566224882857	28.115630508695894	23.120577348995816
38	20.865872828537995	26.45203573778231	26.111532415614942	26.570559018064756
39	22.298196537714006	25.011425443332314	27.756545146823225	24.933832872130456
40	22.017959290266525	23.431198742447908	29.25993260243977	25.290909364845792
41	20.780244780706823	24.17648919981719	27.799484725061145	27.24378129441484
42	21.47832681287886	24.30430349995229	28.857656553684517	25.359713133484334
43	22.954845643516123	23.06156681749926	27.87657507897367	26.10701246001095
44	22.389348975727838	23.80309064519855	28.26102241395762	25.546537965115988
45	22.569896091242835	23.108273025407172	27.513220870141673	26.808610013208316
46	23.39478798897131	24.31485006302827	27.87732840490767	24.413033543092755
47	20.968827372851138	23.864863371786438	30.808770722740903	24.357538532621522
48	21.339463732378448	23.824183771350512	28.12416820261454	26.71218429365649
49	21.457735904016232	23.051773580357278	30.52853347529342	24.961957040333072
50	21.305061848059182	22.447103964001062	29.828442573964054	26.4193916139757
51	21.646569604805215	22.513145537548276	27.552896035998938	28.287388821647575
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	449.5
2	741.0
3	2101.0
4	3461.0
5	2322.0
6	1183.0
7	1122.5
8	1062.0
9	1088.5
10	1115.0
11	1091.0
12	1067.0
13	1045.5
14	1024.0
15	1042.0
16	1060.0
17	959.5
18	859.0
19	926.5
20	994.0
21	1196.0
22	1398.0
23	1424.5
24	1451.0
25	1962.0
26	2755.5
27	3038.0
28	3644.0
29	4250.0
30	5244.5
31	6239.0
32	6437.5
33	6636.0
34	7436.5
35	8237.0
36	8979.0
37	9721.0
38	10448.5
39	11176.0
40	12989.5
41	14803.0
42	16536.5
43	18270.0
44	20491.5
45	22713.0
46	30746.5
47	38780.0
48	38138.0
49	37496.0
50	36330.0
51	35164.0
52	31205.0
53	27246.0
54	24573.0
55	21900.0
56	20688.0
57	19476.0
58	18936.5
59	18397.0
60	17960.0
61	17523.0
62	16492.0
63	15461.0
64	14064.5
65	12668.0
66	11017.5
67	9367.0
68	8038.0
69	6709.0
70	6091.5
71	5474.0
72	4712.5
73	3951.0
74	3251.5
75	1952.5
76	1353.0
77	1099.0
78	845.0
79	655.5
80	466.0
81	313.0
82	160.0
83	119.5
84	79.0
85	52.5
86	26.0
87	18.0
88	10.0
89	5.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.51943830636658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.89879422363954	24.100071972814316
2	8.258815435109087	5.536617089216142
3	3.314278363499106	3.3327824740630168
4	2.082427825480976	2.792072440946827
5	1.5990568007155068	2.679974288997968
6	1.356055415799314	2.7272529509939636
7	1.2254192043802155	2.8752754394461615
8	1.1091857731174681	2.97434272738484
9	1.041363107467076	3.141531579174196
>10	7.985944255606527	41.91498783547745
>50	0.08050629640245764	1.7961589720270195
>100	0.04138174114145019	2.8553934121440485
>500	0.00300958117392365	0.7043909026778243
>1k	0.0037619764674045627	2.5691479146362326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	2497	0.6270182857315046	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	2369	0.5948763792142309	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2172	0.5454079762149892	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	1692	0.4248758267752125	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	1457	0.36586529527865524	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	786	0.1973713947076342	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	762	0.19134478723564538	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	646	0.16221618445436603	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	599	0.15041407815505456	No Hit
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	464	0.1165144111251174	TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	426	0.10697228262780176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1948603082609722	0.0
2	0.0	0.0	0.0	0.9695304770562031	0.0
3	0.0	0.0	0.0	1.306267169553579	0.0
4	0.0	0.0	0.0	1.878794879392518	0.0
5	0.0	0.0	0.0	3.7349899807650777	0.0
6	0.0	0.0	0.0	4.624667908817429	0.0
7	0.0	0.0	0.0	5.56657643496035	0.0
8	0.0	0.0	0.0	6.850997152427969	0.0
9	0.0	0.0	0.0	7.4004228669576175	0.0
10	0.0	0.0	0.0	9.031875731353928	0.0
11	0.0	0.0	0.0	10.354967180100141	0.0
12	0.0	0.0	0.0	11.788295323854818	0.0
13	0.0	0.0	0.0	12.199611283818056	0.0
14	0.0	0.0	0.0	12.38191615984572	0.0
15	0.0	0.0	0.0	12.755816931753692	0.0
16	0.0	0.0	0.0	13.383086326129863	0.0
17	0.0	0.0	0.0	14.089454943575888	0.0
18	0.0	0.0	0.0	14.872160589000437	0.0
19	0.0	0.0	0.0	15.380153377160163	0.0
20	0.0	0.0	0.0	15.817584636168684	0.0
21	0.0	0.0	0.0	16.30975757971444	0.0
22	0.0	0.0	0.0	16.83708573351346	0.0
23	0.0	0.0	0.0	17.355122867459833	0.0
24	0.0	0.0	0.0	17.77271654353973	0.0
25	0.0	0.0	0.0	18.13431299185906	0.0
26	0.0	0.0	0.0	18.49189170186373	0.0
27	0.0	0.0	0.0	18.876590145492347	0.0
28	0.0	0.0	0.0	19.220106771395713	0.0
29	0.0	0.0	0.0	19.601791911288338	0.0
30	0.0	0.0	0.0	20.01160121938358	0.0
31	0.0	0.0	0.0	20.387259751804216	0.0
32	0.0	0.0	0.0	20.760658306422858	0.0
33	0.0	0.0	0.0	21.1154748213362	0.0
34	0.0	0.0	0.0	21.44995153603158	0.0
35	0.0	0.0	0.0	21.82862337218821	0.0
36	0.0	0.0	0.0	22.183942104390887	0.0
37	0.0	0.0	0.0	22.522185448756257	0.0
38	0.0	0.0	0.0	22.858671032608967	0.0
39	0.0	0.0	0.0	23.183605618807032	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.209246E-7	45.000004	6
ATACCGG	35	1.209246E-7	45.000004	2
CGGCGAA	20	7.0277276E-4	45.0	31
TCGTTCA	25	3.8861985E-5	45.0	16
CAACGAT	20	7.0277276E-4	45.0	37
GCGAAAT	20	7.0277276E-4	45.0	17
CGTTCAT	25	3.8861985E-5	45.0	17
TGATTCG	25	3.8861985E-5	45.0	15
TGCGAAA	20	7.0277276E-4	45.0	16
GTACTAG	25	3.8861985E-5	45.0	1
GCGATAC	25	3.8861985E-5	45.0	9
CTCGAAT	20	7.0277276E-4	45.0	43
TCGAATG	20	7.0277276E-4	45.0	44
ACACGAC	50	1.0786607E-9	40.5	26
TTAATAG	45	1.9228537E-8	40.000004	1
TAGTTAA	75	0.0	39.0	38
CGTTTTT	1035	0.0	38.260868	1
GCGAAAG	30	1.13858245E-4	37.499996	1
CGCATTG	30	1.13858245E-4	37.499996	24
GCCGATT	30	1.13858245E-4	37.499996	9
>>END_MODULE