##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551991_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608316
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.239055030609094	33.0	31.0	34.0	30.0	34.0
2	32.35713510741128	34.0	31.0	34.0	30.0	34.0
3	32.317409043983716	34.0	31.0	34.0	30.0	34.0
4	35.91796862157168	37.0	35.0	37.0	35.0	37.0
5	35.971169589489676	37.0	35.0	37.0	35.0	37.0
6	35.9371083450049	37.0	35.0	37.0	35.0	37.0
7	36.09660275251679	37.0	35.0	37.0	35.0	37.0
8	35.913622196358475	37.0	35.0	37.0	35.0	37.0
9	37.80821974105564	39.0	38.0	39.0	35.0	39.0
10	37.57797098876242	39.0	37.0	39.0	35.0	39.0
11	37.52293216025881	39.0	37.0	39.0	35.0	39.0
12	37.43824755554679	39.0	37.0	39.0	35.0	39.0
13	37.41199146496229	39.0	37.0	39.0	35.0	39.0
14	38.71454967484005	40.0	38.0	41.0	35.0	41.0
15	38.73006134969325	40.0	38.0	41.0	35.0	41.0
16	38.74677963426903	40.0	38.0	41.0	35.0	41.0
17	38.655445525023175	40.0	38.0	41.0	35.0	41.0
18	38.440322135206046	40.0	38.0	41.0	34.0	41.0
19	38.24716265888124	40.0	37.0	41.0	34.0	41.0
20	37.986768390113035	40.0	35.0	41.0	34.0	41.0
21	37.92179229216394	40.0	35.0	41.0	34.0	41.0
22	37.92004813287831	40.0	35.0	41.0	34.0	41.0
23	37.86249745198219	40.0	35.0	41.0	34.0	41.0
24	37.79506210587918	40.0	35.0	41.0	34.0	41.0
25	37.63995686452436	40.0	35.0	41.0	33.0	41.0
26	37.63426245569737	40.0	35.0	41.0	33.0	41.0
27	37.610975216828095	40.0	35.0	41.0	33.0	41.0
28	37.48734046120766	40.0	35.0	41.0	33.0	41.0
29	37.38708664575648	39.0	35.0	41.0	33.0	41.0
30	37.16166433235358	39.0	35.0	41.0	33.0	41.0
31	36.97271483899815	39.0	35.0	41.0	32.0	41.0
32	36.64686610248621	39.0	35.0	41.0	31.0	41.0
33	36.31795152519414	39.0	35.0	41.0	30.0	41.0
34	35.98679962387969	39.0	35.0	41.0	28.0	41.0
35	35.77884849321734	39.0	35.0	41.0	26.0	41.0
36	35.597234003379825	39.0	35.0	41.0	25.0	41.0
37	35.48261758691206	39.0	35.0	41.0	24.0	41.0
38	35.359265579074034	39.0	35.0	41.0	24.0	41.0
39	35.29722216742614	39.0	35.0	41.0	23.0	41.0
40	35.1955825590647	39.0	35.0	41.0	23.0	41.0
41	35.075475575194474	38.0	35.0	41.0	23.0	41.0
42	34.91954017319946	38.0	35.0	41.0	23.0	41.0
43	34.79746381814715	38.0	35.0	41.0	22.0	41.0
44	34.81031897895173	38.0	35.0	41.0	22.0	41.0
45	34.79329328835671	38.0	35.0	40.0	23.0	41.0
46	34.72502284996614	38.0	34.0	40.0	23.0	41.0
47	34.63130017951197	37.0	34.0	40.0	23.0	41.0
48	34.603873644618915	37.0	34.0	40.0	23.0	41.0
49	34.54843370879609	37.0	34.0	40.0	23.0	41.0
50	34.42358248015834	37.0	34.0	40.0	23.0	41.0
51	33.62648853556375	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	4.0
9	6.0
10	11.0
11	11.0
12	9.0
13	17.0
14	28.0
15	29.0
16	74.0
17	97.0
18	216.0
19	413.0
20	858.0
21	1420.0
22	2085.0
23	2918.0
24	4354.0
25	6637.0
26	10389.0
27	12650.0
28	12529.0
29	11060.0
30	10731.0
31	11619.0
32	13795.0
33	17985.0
34	32736.0
35	46238.0
36	48327.0
37	64424.0
38	108605.0
39	187824.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.799544973336225	17.68686011875407	21.420610340678202	21.092984567231504
2	37.87554494703411	25.294090571347787	21.515462358379526	15.31490212323858
3	28.19685821185042	24.958902938604275	31.660354158036284	15.183884691509016
4	25.540015386739785	22.456256287850394	34.85934941707928	17.144378908330538
5	25.45765687570276	25.89887492684723	30.14650280446347	18.49696539298654
6	23.946271345813688	32.80515390027551	29.223298417269973	14.025276336640824
7	70.04024224251869	5.809809375390422	19.790865273969448	4.359083108121437
8	70.30967457702904	7.7502482262508305	17.079774327816462	4.860302868903662
9	64.12012835434216	7.571886979793396	18.887058699754732	9.420925966109719
10	38.36131221273154	21.724071042024214	24.798295622669798	15.116321122574453
11	29.563910862117716	21.247180741588252	29.903043812755215	19.28586458353882
12	24.611057410950888	19.165532387772146	34.97524313021522	21.24816707106175
13	23.294143175586374	19.627956522596808	37.66841575759967	19.40948454421715
14	20.16649241512635	23.764457946199016	35.08768469019391	20.981364948480724
15	19.671026242939522	21.33907377086909	38.307721644671524	20.68217834151987
16	21.527298312061493	21.427843423483846	35.173002189651434	21.87185607480323
17	21.712399476587827	22.18896100053262	34.41730942470689	21.681330098172662
18	21.86692442743574	21.841608637615977	33.72770073448668	22.5637662004616
19	22.30880003156254	23.383241604692298	31.75586372871994	22.552094635025217
20	24.501081674655936	22.888268597242224	32.79118089940097	19.819468828700874
21	23.475792186955463	24.71643027636952	32.034008640246185	19.77376889642883
22	22.29022415981168	21.417486964012124	32.71424720046818	23.57804167570802
23	23.328335930667613	22.735058752358974	32.5891477455796	21.347457571393814
24	22.791608308839486	22.89484412706554	32.102887315145416	22.21066024894956
25	22.257182122449517	24.937039301941756	30.851892766259642	21.95388580934909
26	22.095588477041538	22.73900407025296	32.438403724380095	22.72700372832541
27	24.508150369216	22.020791825301323	31.408840142294466	22.06221766318821
28	21.988571729167077	23.822322608644193	32.44103393630942	21.74807172587931
29	24.828871836348213	22.227427849999014	31.259575615305202	21.68412469834757
30	24.47182056694218	23.75262199251705	30.681750932081354	21.09380650845942
31	24.67319616778122	24.038493151585687	28.547827116169884	22.740483564463208
32	25.367900893614504	25.59837321392171	28.350068056733672	20.683657835730113
33	24.635058094805988	24.63620881252507	27.47831719040762	23.250415902261327
34	23.726319873223787	25.2199514725899	29.046252276777203	22.00747637740911
35	25.287843818015638	25.34307826853149	27.86101302612458	21.508064887328295
36	24.919285371418802	27.997784046449542	26.584045134436707	20.49888544769495
37	24.029944962815378	27.045647328033457	28.619500391244024	20.30490731790714
38	22.789471261646906	27.336450134469587	26.92400002630212	22.95007857758139
39	24.31482979241052	25.289652088717045	28.542731080556816	21.85278703831561
40	22.771059778141623	25.337489068181668	30.041951880272755	21.849499273403953
41	22.34611616330986	25.62286706251356	29.074198278526293	22.956818495650285
42	22.949256636353475	26.06490705488595	29.518868482828005	21.466967825932574
43	24.457847566067635	23.615357807455336	28.975894107667727	22.950900518809302
44	23.514752201158608	24.0518085994779	28.875124113125416	23.558315086238075
45	22.973750484945324	23.39688582907568	28.985099849420365	24.64426383655863
46	24.077288777543252	24.902517770369347	28.41763162566824	22.602561826419162
47	21.9921882705699	24.488095003254887	31.538213691568195	21.981503034607012
48	22.592369755193026	23.897776813366736	30.038992891852264	23.470860539587974
49	22.15476824545138	23.766266216900426	31.51158279578377	22.567382741864424
50	21.505105898907807	23.195017063499893	31.202039729351196	24.09783730824111
51	21.822868377619525	23.18285233332676	29.727970331209434	25.26630895784428
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	376.0
1	753.0
2	1130.0
3	11773.5
4	22417.0
5	14384.0
6	6351.0
7	6142.5
8	5934.0
9	5823.5
10	5713.0
11	5489.5
12	5266.0
13	5075.5
14	4885.0
15	4505.0
16	4125.0
17	3842.5
18	3560.0
19	3282.5
20	3005.0
21	2957.0
22	2909.0
23	2963.0
24	3017.0
25	3354.0
26	4445.5
27	5200.0
28	5828.0
29	6456.0
30	7253.0
31	8050.0
32	8689.5
33	9329.0
34	10186.0
35	11043.0
36	11790.5
37	12538.0
38	13284.0
39	14030.0
40	16075.0
41	18120.0
42	19985.0
43	21850.0
44	24480.0
45	27110.0
46	39868.5
47	52627.0
48	49132.0
49	45637.0
50	44374.5
51	43112.0
52	39491.0
53	35870.0
54	34062.5
55	32255.0
56	31067.0
57	29879.0
58	29876.5
59	29874.0
60	29514.5
61	29155.0
62	27592.5
63	26030.0
64	23761.5
65	21493.0
66	18552.0
67	15611.0
68	13816.5
69	12022.0
70	10837.0
71	9652.0
72	8351.0
73	7050.0
74	6103.5
75	4100.0
76	3043.0
77	2383.0
78	1723.0
79	1443.0
80	1163.0
81	868.5
82	574.0
83	379.0
84	184.0
85	122.0
86	60.0
87	42.5
88	25.0
89	18.5
90	12.0
91	6.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	608316.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.977187942709584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78241886383391	25.1074715558262
2	7.413833807721981	5.1863011693741194
3	3.506547496863763	3.6794751248352506
4	2.4899022737664565	3.483591191540371
5	2.0584762986909855	3.5999856187463894
6	1.7501675013853357	3.67295625763064
7	1.591228684497993	3.8959693330261786
8	1.448297921674633	4.052591088275945
9	1.2405683882304361	3.905243430382825
>10	6.62191257364351	33.62366010989649
>50	0.058745723146931605	1.4191101892660556
>100	0.02889910573985307	2.04614346289458
>500	0.00615882581341131	1.3816605275036449
>1k	0.00189502332720348	1.5781883981703901
>5k	4.7375583180087E-4	0.9054201393955283
>10k+	4.7375583180087E-4	2.4622324032353875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14859	2.4426449411161304	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	5464	0.8982173738648992	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	3710	0.6098803911125139	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	2867	0.47130110008613946	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	1668	0.27419959363225693	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1279	0.21025256610051357	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	975	0.16027853944331566	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	953	0.1566619980404921	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	724	0.11901708980201078	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	662	0.10882501857587175	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	640	0.10520847717304822	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.575529823315513E-4	0.0	0.0	0.08597505243985035	0.0
2	6.575529823315513E-4	0.0	0.0	0.4380946744783961	0.0
3	6.575529823315513E-4	0.0	0.0	0.6614983002255407	0.0
4	6.575529823315513E-4	0.0	0.0	1.2197607822250278	0.0
5	6.575529823315513E-4	0.0	0.0	2.8516428961263554	0.0
6	6.575529823315513E-4	0.0	0.0	3.821040380328645	0.0
7	6.575529823315513E-4	0.0	0.0	4.639529455085843	0.0
8	6.575529823315513E-4	0.0	0.0	5.71462858119793	0.0
9	6.575529823315513E-4	0.0	0.0	6.3406190203775665	0.0
10	6.575529823315513E-4	0.0	0.0	7.815345971501654	0.0
11	6.575529823315513E-4	0.0	0.0	8.713070180629805	0.0
12	6.575529823315513E-4	0.0	0.0	9.887459807073954	0.0
13	6.575529823315513E-4	0.0	0.0	10.244839852971154	0.0
14	6.575529823315513E-4	0.0	0.0	10.421885993463924	0.0
15	6.575529823315513E-4	0.0	0.0	10.705620105339987	0.0
16	8.219412279144392E-4	0.0	0.0	11.03932824387325	0.0
17	8.219412279144392E-4	0.0	0.0	11.399338501699775	0.0
18	8.219412279144392E-4	0.0	0.0	11.816720257234726	0.0
19	8.219412279144392E-4	0.0	0.0	12.165223337870449	0.0
20	8.219412279144392E-4	0.0	0.0	12.448299896764182	0.0
21	8.219412279144392E-4	0.0	0.0	12.72200632565969	0.002137047192577542
22	8.219412279144392E-4	0.0	0.0	13.04305656928307	0.002137047192577542
23	8.219412279144392E-4	0.0	0.0	13.32991405782521	0.002137047192577542
24	8.219412279144392E-4	0.0	0.0	13.591126980056417	0.002137047192577542
25	8.219412279144392E-4	0.0	0.0	13.836229854220504	0.002137047192577542
26	8.219412279144392E-4	0.0	0.0	14.0591403152309	0.002137047192577542
27	8.219412279144392E-4	0.0	0.0	14.299640318518664	0.002137047192577542
28	8.219412279144392E-4	0.0	0.0	14.53389356847428	0.002137047192577542
29	8.219412279144392E-4	0.0	0.0	14.776366230709039	0.002137047192577542
30	8.219412279144392E-4	0.0	0.0	15.056976965919029	0.002137047192577542
31	8.219412279144392E-4	0.0	0.0	15.28416152131458	0.002137047192577542
32	8.219412279144392E-4	0.0	0.0	15.556059679508676	0.002137047192577542
33	8.219412279144392E-4	0.0	0.0	15.802148883146259	0.002137047192577542
34	8.219412279144392E-4	0.0	0.0	16.037717239066538	0.002137047192577542
35	8.219412279144392E-4	0.0	0.0	16.28775176059811	0.002137047192577542
36	8.219412279144392E-4	0.0	0.0	16.527101046166795	0.002137047192577542
37	8.219412279144392E-4	0.0	0.0	16.771710755594132	0.002137047192577542
38	8.219412279144392E-4	0.0	0.0	17.0284851951946	0.002137047192577542
39	8.219412279144392E-4	0.0	0.0	17.284766470058326	0.002137047192577542
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGT	20	7.030666E-4	45.0	4
CAGTCGA	30	2.1638098E-6	44.999996	41
CGTTTTT	6490	0.0	43.335903	1
TTGTGCG	65	0.0	41.53846	1
GTCGAAT	50	1.0804797E-9	40.500004	43
TAACGTC	85	0.0	39.705883	32
ATGAATG	510	0.0	39.705883	21
GTAGCCG	40	3.454643E-7	39.375	43
CATCGTT	40	3.454643E-7	39.375	36
GATGAAT	540	0.0	39.166664	20
CGATGAA	535	0.0	38.69159	19
GCAAACG	35	6.243439E-6	38.57143	1
CGACAGG	70	0.0	38.57143	2
ATAACGC	35	6.243439E-6	38.57143	11
GTTTTTT	7560	0.0	38.3631	2
GCGATTC	65	9.094947E-12	38.07692	9
CGTAACC	130	0.0	38.07692	35
CCGATGA	540	0.0	37.916664	18
TACGGCT	430	0.0	37.67442	7
TGAATGA	545	0.0	36.74312	22
>>END_MODULE