FastQCFastQC Report
Sat 18 Jun 2016
SRR3551990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303967
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35391.1642711215362194No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG14090.4635371602838466No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC13400.4408373277362345No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC11880.3908318995154079No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC8830.2904920599933546No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC5580.18357255886329765No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC5100.16778137100408927No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT4730.1556089970292828Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4400.144752555376077No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3720.12238170590886512No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3480.11448611197926091No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3320.1092223826928581No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT3220.10593255188885635No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA3200.10527458572805601No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTT207.0250913E-445.04
TAACGCC207.0250913E-445.012
TGTGACG207.0250913E-445.01
TACGCGG207.0250913E-445.02
TGATTCG253.884014E-545.015
TGATTCA406.7866495E-945.044
ACTAAGG253.884014E-545.02
ATGGTCG207.0250913E-445.044
TTAGTCA406.7866495E-945.032
CGAATAT600.044.99999614
CGATGGT600.044.99999610
CGCCCTT302.1602154E-644.99999629
AGGGCTA750.042.06
AGGGATA700.041.7857136
AATATAC650.041.5384616
TACGAAT650.041.5384612
CGTTTTT14700.041.4795881
CGTGGGA451.9203071E-840.04
GGTATAA451.9203071E-840.027
ACGGGAT850.039.7058835
Produced by FastQC (version 0.11.5)