##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551990_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 303967 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.298502798001095 33.0 31.0 34.0 30.0 34.0 2 32.38626561435945 34.0 31.0 34.0 30.0 34.0 3 32.383291607312636 34.0 31.0 34.0 30.0 34.0 4 35.94302013047469 37.0 35.0 37.0 35.0 37.0 5 36.00137185944527 37.0 35.0 37.0 35.0 37.0 6 35.99241036033517 37.0 35.0 37.0 35.0 37.0 7 36.148071994657315 37.0 36.0 37.0 35.0 37.0 8 35.98174472886859 37.0 35.0 37.0 35.0 37.0 9 37.885017123569334 39.0 38.0 39.0 35.0 39.0 10 37.62506127309872 39.0 37.0 39.0 35.0 39.0 11 37.60232196258146 39.0 37.0 39.0 35.0 39.0 12 37.4775551293397 39.0 37.0 39.0 35.0 39.0 13 37.41721963239431 39.0 37.0 39.0 35.0 39.0 14 38.693739780963064 40.0 38.0 41.0 35.0 41.0 15 38.74532761780062 40.0 38.0 41.0 35.0 41.0 16 38.75889816986712 40.0 38.0 41.0 35.0 41.0 17 38.70434619547517 40.0 38.0 41.0 35.0 41.0 18 38.60137778114071 40.0 38.0 41.0 35.0 41.0 19 38.45921103277659 40.0 37.0 41.0 35.0 41.0 20 38.30567462915382 40.0 37.0 41.0 34.0 41.0 21 38.20195942322686 40.0 36.0 41.0 34.0 41.0 22 38.210980139291436 40.0 36.0 41.0 34.0 41.0 23 38.16100431954784 40.0 36.0 41.0 34.0 41.0 24 38.09266137442551 40.0 36.0 41.0 34.0 41.0 25 37.91973141821316 40.0 36.0 41.0 34.0 41.0 26 37.93583185016794 40.0 36.0 41.0 34.0 41.0 27 37.9152407991657 40.0 36.0 41.0 34.0 41.0 28 37.82970190843085 40.0 35.0 41.0 34.0 41.0 29 37.751321689525504 40.0 35.0 41.0 33.0 41.0 30 37.60398990679909 40.0 35.0 41.0 33.0 41.0 31 37.54223320294637 40.0 35.0 41.0 33.0 41.0 32 37.34728111933203 40.0 35.0 41.0 33.0 41.0 33 37.164942247020235 40.0 35.0 41.0 33.0 41.0 34 36.995015906331936 40.0 35.0 41.0 32.0 41.0 35 36.86650195580442 40.0 35.0 41.0 32.0 41.0 36 36.7283784094984 39.0 35.0 41.0 31.0 41.0 37 36.65670944543322 39.0 35.0 41.0 31.0 41.0 38 36.54863192386016 39.0 35.0 41.0 31.0 41.0 39 36.4683501827501 39.0 35.0 41.0 31.0 41.0 40 36.366858902446644 39.0 35.0 41.0 30.0 41.0 41 36.25967621485227 39.0 35.0 41.0 30.0 41.0 42 36.14742060815812 39.0 35.0 41.0 30.0 41.0 43 35.99826625916629 39.0 35.0 41.0 30.0 41.0 44 36.03388854711202 39.0 35.0 41.0 30.0 41.0 45 36.017656521925076 39.0 35.0 41.0 30.0 41.0 46 35.93502913145177 38.0 35.0 41.0 30.0 41.0 47 35.85756677534074 38.0 35.0 41.0 30.0 41.0 48 35.8094365506782 38.0 35.0 41.0 30.0 41.0 49 35.7522987692743 38.0 35.0 40.0 30.0 41.0 50 35.67893554234506 38.0 35.0 40.0 29.0 41.0 51 34.852256988423086 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 7.0 11 7.0 12 9.0 13 8.0 14 12.0 15 17.0 16 28.0 17 44.0 18 81.0 19 149.0 20 262.0 21 434.0 22 683.0 23 974.0 24 1508.0 25 2258.0 26 3191.0 27 3945.0 28 4035.0 29 3899.0 30 3989.0 31 4864.0 32 6017.0 33 8399.0 34 16531.0 35 24906.0 36 22408.0 37 33256.0 38 56388.0 39 105533.0 40 121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.59497577039613 19.582388877740016 23.32983514657841 15.492800205285443 2 34.12870476071416 25.911694361558986 23.62756483434057 16.33203604338629 3 30.654314448607906 24.560231867275064 28.518885273730373 16.266568410386654 4 27.949086578477267 23.93154520063033 30.004243881737164 18.115124339155237 5 26.144285399401905 28.28497830356585 26.08934522497508 19.481391072057164 6 24.98198817634809 34.900499067332966 25.193195313965006 14.92431744235394 7 74.36563837521837 6.680659413686355 14.159102797343131 4.794599413752151 8 74.4396595683084 7.757092052755726 12.532939430925067 5.270308948010804 9 68.13108001855464 8.466379574098504 14.464070112874094 8.938470294472756 10 39.69147966720072 23.149552418519114 20.73152677757783 16.42744113670234 11 31.08758516549494 22.081015373379348 26.235742695753157 20.595656765372556 12 26.827912240473474 19.975194675737825 31.819243536304924 21.377649547483774 13 24.069060128237606 22.38861455355351 33.77307405080157 19.769251267407316 14 21.100316810706428 26.658156970986983 30.649050719321508 21.592475498985088 15 19.665621597081262 22.661999493366057 35.49299759513368 22.179381314419 16 21.345080222524153 22.68634424131567 32.28574154431237 23.682833991847797 17 21.923432477867664 22.9337395177766 30.209858306987268 24.932969697368463 18 22.443554727980338 22.3695335348903 31.185293140373794 24.00161859675557 19 23.74106399707863 23.672635516355395 29.361739925715618 23.224560560850353 20 24.729329170600757 23.844035701243886 29.306470768208392 22.120164359946966 21 24.41745321038139 25.35012024331589 29.449249425102064 20.78317712120066 22 22.57448999397961 22.376113196498302 29.15941533126951 25.889981478252572 23 22.7396395003405 24.803021380610396 28.90544039320058 23.55189872584853 24 23.05118647747946 23.799294002309463 29.662759444281782 23.486760075929293 25 22.025088249711317 26.33970134915961 28.432691706665526 23.202518694463546 26 21.0499823994052 25.239252945221025 29.499254853322892 24.21150980205088 27 24.77341290337438 24.384554902341375 28.142857612832973 22.699174581451274 28 21.418772432533796 24.406925751808583 30.738205134109954 23.436096681547667 29 22.215898436343416 24.6217517033099 28.938667684320997 24.223682176025687 30 23.789424509897454 25.48171347547596 28.271489997269438 22.457372017357148 31 24.749397138505167 24.744791375379563 25.73832027818809 24.767491207927176 32 24.77374188645478 25.617583487681227 26.891076991910307 22.717597633953684 33 24.279609299693718 23.868051466113098 25.951501314287405 25.90083791990578 34 22.302420986488663 24.688864251711536 27.736563508538758 25.272151253261043 35 25.09844818680975 23.57262465991374 26.93910852164873 24.389818631627776 36 24.36152608671336 25.519217546641578 26.915750722940317 23.203505643704744 37 23.885487569374305 24.953695631433675 28.843262591004944 22.317554208187072 38 22.82089832119934 25.329723292331074 26.478861192168885 25.370517194300696 39 23.36931311622643 23.25186615652357 29.329828566916806 24.048992160333192 40 23.38313040560324 23.179818861915933 29.64433639177937 23.79271434070146 41 22.02772011435452 24.03978063408199 28.443548148318733 25.488951103244762 42 23.260748699694375 24.11873657337803 28.947879210572204 23.672635516355395 43 23.714087384485815 22.27708928929785 28.998871588034227 25.009951738182107 44 22.972559521263822 23.280158701437987 28.616922231689625 25.13035954560857 45 23.229495307056357 23.023222915645448 28.46230018390154 25.28498159339665 46 24.45528626462741 23.989775205861164 28.496843407343558 23.058095122167867 47 22.768261028335314 22.864982053972966 30.712544453838742 23.654212463852982 48 22.452766254231545 23.216993950001154 29.529192313639307 24.801047482127995 49 22.135626564725776 22.975520368987425 31.044159398882115 23.844693667404684 50 21.718476018778354 22.5590277892008 30.586543934045473 25.135952257975376 51 22.920251211480196 22.567910332371607 28.08331167528054 26.42852678086766 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 213.0 1 437.5 2 662.0 3 3062.0 4 5462.0 5 3569.5 6 1677.0 7 1637.0 8 1597.0 9 1583.5 10 1570.0 11 1541.0 12 1512.0 13 1506.0 14 1500.0 15 1402.5 16 1305.0 17 1190.5 18 1076.0 19 1014.0 20 952.0 21 1005.0 22 1058.0 23 1232.5 24 1407.0 25 1698.0 26 2182.0 27 2375.0 28 2635.0 29 2895.0 30 3411.0 31 3927.0 32 4307.5 33 4688.0 34 5065.5 35 5443.0 36 5836.0 37 6229.0 38 6738.5 39 7248.0 40 8749.0 41 10250.0 42 11578.5 43 12907.0 44 14754.0 45 16601.0 46 21610.5 47 26620.0 48 26722.0 49 26824.0 50 26066.5 51 25309.0 52 22479.0 53 19649.0 54 18422.0 55 17195.0 56 16288.5 57 15382.0 58 14527.5 59 13673.0 60 13908.5 61 14144.0 62 12976.5 63 11809.0 64 11121.5 65 10434.0 66 9325.0 67 8216.0 68 7169.0 69 6122.0 70 5233.5 71 4345.0 72 4212.0 73 4079.0 74 3305.0 75 2082.0 76 1633.0 77 1150.0 78 667.0 79 574.5 80 482.0 81 342.5 82 203.0 83 138.0 84 73.0 85 44.5 86 16.0 87 15.0 88 14.0 89 7.5 90 1.0 91 1.5 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 303967.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.17934292346292 #Duplication Level Percentage of deduplicated Percentage of total 1 73.16041230219692 24.274144081965265 2 8.171925202036306 5.422782172465031 3 3.0878508482543428 3.0735858657221007 4 1.7978642199626886 2.3860781393586485 5 1.3998931180783498 2.3223766910458665 6 1.159911411548512 2.30910590907636 7 1.0389206514473979 2.4129493195248495 8 0.9469276774762977 2.51347505077635 9 0.8869322596841441 2.648504663454788 >10 8.211372848394625 44.574038212342884 >50 0.08299366127822136 1.8635413622228754 >100 0.047996334233188255 3.071840746452128 >500 0.002999770889574266 0.6472795438306622 >1k 0.003999694519432355 2.4802982417621893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3539 1.1642711215362194 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG 1409 0.4635371602838466 No Hit GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC 1340 0.4408373277362345 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC 1188 0.3908318995154079 No Hit GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC 883 0.2904920599933546 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC 558 0.18357255886329765 No Hit CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC 510 0.16778137100408927 No Hit CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT 473 0.1556089970292828 Illumina Single End Adapter 1 (95% over 21bp) GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 440 0.144752555376077 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 372 0.12238170590886512 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 348 0.11448611197926091 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 332 0.1092223826928581 No Hit GAATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT 322 0.10593255188885635 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA 320 0.10527458572805601 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15758289551168383 0.0 2 0.0 0.0 0.0 0.7974549868900243 0.0 3 0.0 0.0 0.0 1.0849861991597771 0.0 4 0.0 0.0 0.0 1.5745130227952377 0.0 5 0.0 0.0 0.0 3.1079031605404532 0.0 6 0.0 0.0 0.0 3.8898959426516693 0.0 7 0.0 0.0 0.0 4.66037431694888 0.0 8 0.0 0.0 0.0 5.820039675359496 0.0 9 0.0 0.0 0.0 6.280615987919742 0.0 10 0.0 0.0 0.0 7.699191030605296 0.0 11 0.0 0.0 0.0 8.96840775478917 0.0 12 0.0 0.0 0.0 10.342241098540303 0.0 13 0.0 0.0 0.0 10.769919103060529 0.0 14 0.0 0.0 0.0 10.972243697506638 0.0 15 0.0 0.0 0.0 11.342349662956835 0.0 16 0.0 0.0 0.0 11.982879720495974 0.0 17 0.0 0.0 0.0 12.667164527728339 0.0 18 0.0 0.0 0.0 13.488306296407176 0.0 19 0.0 0.0 0.0 13.996585155625446 0.0 20 0.0 0.0 0.0 14.444002144969684 0.0 21 0.0 0.0 0.0 14.94306947793675 0.0 22 0.0 0.0 0.0 15.465494609612227 0.0 23 0.0 0.0 0.0 15.950086687041685 0.0 24 0.0 0.0 0.0 16.375790793079513 0.0 25 0.0 0.0 0.0 16.742277944645306 0.0 26 0.0 0.0 0.0 17.08869712830669 0.0 27 0.0 0.0 0.0 17.454197330631285 0.0 28 0.0 0.0 0.0 17.79864261581027 0.0 29 0.0 0.0 0.0 18.138811120944048 0.0 30 0.0 0.0 0.0 18.510233018715848 0.0 31 0.0 0.0 0.0 18.85731016853803 0.0 32 0.0 0.0 0.0 19.220507489299827 0.0 33 0.0 0.0 0.0 19.585020742383218 0.0 34 0.0 0.0 0.0 19.968943997210225 0.0 35 0.0 0.0 0.0 20.363065727529634 0.0 36 0.0 0.0 0.0 20.712116775834218 0.0 37 0.0 0.0 0.0 21.05393019637 0.0 38 0.0 0.0 0.0 21.399033447709783 0.0 39 0.0 0.0 0.0 21.720778900341156 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGGTT 20 7.0250913E-4 45.0 4 TAACGCC 20 7.0250913E-4 45.0 12 TGTGACG 20 7.0250913E-4 45.0 1 TACGCGG 20 7.0250913E-4 45.0 2 TGATTCG 25 3.884014E-5 45.0 15 TGATTCA 40 6.7866495E-9 45.0 44 ACTAAGG 25 3.884014E-5 45.0 2 ATGGTCG 20 7.0250913E-4 45.0 44 TTAGTCA 40 6.7866495E-9 45.0 32 CGAATAT 60 0.0 44.999996 14 CGATGGT 60 0.0 44.999996 10 CGCCCTT 30 2.1602154E-6 44.999996 29 AGGGCTA 75 0.0 42.0 6 AGGGATA 70 0.0 41.785713 6 AATATAC 65 0.0 41.53846 16 TACGAAT 65 0.0 41.53846 12 CGTTTTT 1470 0.0 41.479588 1 CGTGGGA 45 1.9203071E-8 40.0 4 GGTATAA 45 1.9203071E-8 40.0 27 ACGGGAT 85 0.0 39.705883 5 >>END_MODULE