FastQCFastQC Report
Sat 18 Jun 2016
SRR3551988_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551988_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470123
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84731.8022942931956105No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC24380.5185876887537942No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG21550.4583906764825375No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC16810.3575659986854504No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC11100.23610842268938131No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC9730.20696711286195316No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT6580.13996337128793954No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC5800.12337196861246952No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT5010.10656785564628832No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT4880.10380262186704331No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTG4780.10167551895993177No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT4760.10125009837850946No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4740.10082467779708715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG207.029027E-445.0000041
TAGTGCG207.029027E-445.0000041
ATTTCGT207.029027E-445.00000412
CGTTTTT37450.042.476631
GTTAATC451.924127E-840.040
TAATGCG356.240414E-638.5714261
ATACTAT659.094947E-1238.07692745
ATTGGGC1600.037.9687544
AGATCGG301.13889575E-437.4999962
CGAGGGT601.546141E-1037.4999964
CATATGC1450.037.24138333
CGGGTAC800.036.5625046
GGTACCT1600.036.5625048
GTTTTTT45150.036.17942
TATGGGC1750.036.0000044
TTGATCC1500.036.017
CGGCGAA250.00210584536.031
CGAAAGG751.8189894E-1236.02
TAGCGCG250.00210584536.01
ACCGAGT250.00210584536.028