##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551988_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470123
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.246724793298775	33.0	31.0	34.0	30.0	34.0
2	32.37100078064677	34.0	31.0	34.0	30.0	34.0
3	32.34241251757519	34.0	31.0	34.0	30.0	34.0
4	35.92299249345384	37.0	35.0	37.0	35.0	37.0
5	35.98485290019846	37.0	35.0	37.0	35.0	37.0
6	35.97206050331509	37.0	35.0	37.0	35.0	37.0
7	36.15661645994771	37.0	36.0	37.0	35.0	37.0
8	36.01003354441284	37.0	36.0	37.0	35.0	37.0
9	37.89794160251679	39.0	38.0	39.0	35.0	39.0
10	37.566626180808	39.0	37.0	39.0	35.0	39.0
11	37.534243166150134	39.0	37.0	39.0	35.0	39.0
12	37.471178819160095	39.0	37.0	39.0	35.0	39.0
13	37.454827779113124	39.0	37.0	39.0	35.0	39.0
14	38.738334435881676	40.0	38.0	41.0	35.0	41.0
15	38.7738464189159	40.0	38.0	41.0	35.0	41.0
16	38.77206816088555	40.0	38.0	41.0	35.0	41.0
17	38.66261808080013	40.0	38.0	41.0	35.0	41.0
18	38.504780663783734	40.0	38.0	41.0	34.0	41.0
19	38.34296769143394	40.0	37.0	41.0	34.0	41.0
20	38.14760392493029	40.0	36.0	41.0	34.0	41.0
21	38.06307498250458	40.0	36.0	41.0	34.0	41.0
22	38.08670496869968	40.0	36.0	41.0	34.0	41.0
23	38.04221023008872	40.0	35.0	41.0	34.0	41.0
24	37.977305939084026	40.0	35.0	41.0	34.0	41.0
25	37.83560897892679	40.0	35.0	41.0	34.0	41.0
26	37.840571510009084	40.0	35.0	41.0	34.0	41.0
27	37.837419143500746	40.0	35.0	41.0	34.0	41.0
28	37.73120651403994	40.0	35.0	41.0	34.0	41.0
29	37.66488982670493	40.0	35.0	41.0	33.0	41.0
30	37.45717610072258	40.0	35.0	41.0	33.0	41.0
31	37.33328086479496	40.0	35.0	41.0	33.0	41.0
32	37.078470953346255	40.0	35.0	41.0	32.0	41.0
33	36.82914471319208	40.0	35.0	41.0	31.0	41.0
34	36.55909410941392	40.0	35.0	41.0	31.0	41.0
35	36.389021596475814	40.0	35.0	41.0	30.0	41.0
36	36.26133160896191	39.0	35.0	41.0	30.0	41.0
37	36.228308336329	39.0	35.0	41.0	30.0	41.0
38	36.111836689547204	39.0	35.0	41.0	29.0	41.0
39	36.070962280084146	39.0	35.0	41.0	29.0	41.0
40	35.967321318038046	39.0	35.0	41.0	29.0	41.0
41	35.8413840633196	39.0	35.0	41.0	28.0	41.0
42	35.718241821821096	39.0	35.0	41.0	27.0	41.0
43	35.62051846006258	39.0	35.0	41.0	27.0	41.0
44	35.634731761687895	39.0	35.0	41.0	27.0	41.0
45	35.585087306938824	39.0	35.0	41.0	27.0	41.0
46	35.5106642304248	38.0	35.0	41.0	27.0	41.0
47	35.416108124894976	38.0	35.0	41.0	26.0	41.0
48	35.37255356576896	38.0	35.0	41.0	26.0	41.0
49	35.33004554127324	38.0	35.0	40.0	27.0	41.0
50	35.23388560015145	38.0	35.0	40.0	26.0	41.0
51	34.41254097331975	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	7.0
11	6.0
12	13.0
13	11.0
14	10.0
15	22.0
16	48.0
17	85.0
18	154.0
19	298.0
20	512.0
21	922.0
22	1404.0
23	1859.0
24	2615.0
25	4127.0
26	6204.0
27	7632.0
28	7694.0
29	7124.0
30	7355.0
31	8359.0
32	9869.0
33	13213.0
34	25312.0
35	35594.0
36	34406.0
37	47122.0
38	85524.0
39	162444.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.91529876223882	18.715527638511624	22.646413810853755	18.722759788395805
2	36.521718784233066	23.955007519308776	23.12139589001176	16.4018778064464
3	29.458886291459898	23.924802658027794	30.957004868938554	15.65930618157376
4	26.138478653458776	23.042054951576503	32.7790812191703	18.04038517579442
5	25.320607585674388	26.481793062666583	29.54779919297716	18.649800158681877
6	24.35511557613646	32.522339898281935	28.545295592855485	14.577248932726118
7	73.77835162287316	5.540252231862725	16.123652746196207	4.5577433990679035
8	75.04036177766244	6.182637309810411	14.223511719273466	4.5534891932536805
9	69.51031963975385	6.153921420564405	16.21979779759765	8.115961142084092
10	36.33580999015151	23.298796272464866	23.294967487232064	17.070426250151556
11	28.691427562574052	23.76378096795945	28.923069069158498	18.621722400308006
12	25.570967597841417	20.67139876160069	32.952652816390604	20.804980824167295
13	23.818022092090793	20.859009238007925	35.35564097055451	19.967327699346768
14	20.573764738164268	24.392552587301623	33.81030070853798	21.223381965996133
15	19.66357740421124	22.670450073704114	36.80185823709966	20.864114284984993
16	22.885925598194515	22.438383146538246	33.40593844589607	21.269752809371166
17	21.67262609997809	23.353462817177633	32.69080644852517	22.283104634319102
18	23.249022064438453	22.33394239379907	32.52446700118905	21.892568540573425
19	22.965053826339066	24.20451669031296	30.8987222492837	21.931707234064277
20	24.770751484186054	24.180905848044024	31.397740591292067	19.650602076477856
21	23.325810479385183	26.142094728400867	31.31308189558903	19.219012896624925
22	22.18079098448704	22.550906890324445	31.985884545108405	23.282417580080107
23	22.33011360856627	24.692899517785772	31.911648653650214	21.065338219997745
24	22.238222762979053	23.770374986971497	31.65639630479683	22.335005945252625
25	22.104002569540313	25.23594888997135	30.283351378256327	22.376697162232013
26	21.435454125835154	24.061149954373644	31.971207535049338	22.532188384741865
27	23.403237025204042	23.373244874213768	30.688139061479657	22.535379039102533
28	20.921971484058428	25.058761217808957	31.733184719743555	22.28608257838906
29	23.534479274572824	23.427273288054405	30.63538690938329	22.402860527989485
30	24.229191084035453	23.28645907560362	31.714466214160975	20.769883626199952
31	24.776494662035255	23.04694728826286	29.78943808322503	22.38711996647686
32	24.726295033427423	23.761228444470913	29.638839197401527	21.87363732470013
33	23.93862882692402	23.412808988286045	29.263405534296343	23.385156650493595
34	23.845887140173954	23.995209764253183	30.095740901849087	22.06316219372377
35	24.53038885568245	24.734590734765156	28.763749061415844	21.97127134813655
36	23.789306202844788	27.694454430010868	28.179859313413726	20.33638005373062
37	23.618499839403732	26.230582209336706	28.373212967670163	21.777704983589402
38	21.8272664813251	26.789159432744196	28.648247373559688	22.73532671237102
39	23.87396489854783	24.900504761519855	28.26366716795392	22.961863171978397
40	24.129855378273348	24.53081427626387	29.90791771515114	21.43141263031164
41	21.756646664808997	27.148001693173914	28.547209985471888	22.5481416565452
42	22.90038989796287	26.435634929582257	28.442981943023423	22.220993229431446
43	23.091829159602913	24.91177840692755	28.709507937284496	23.28688449618504
44	23.362396649387502	23.943733873901085	29.319348340753375	23.374521135958037
45	23.52490731149082	22.79679998638654	29.24404889784163	24.43424380428101
46	23.906296862735925	24.50635259283209	28.68610980530627	22.90124073912572
47	21.964251908543083	25.22020832845872	30.92339664300619	21.892143119992003
48	22.364359965370763	24.54123708050872	30.451179797627432	22.64322315649309
49	22.981219808433114	23.763568257668737	30.69069158496819	22.56452034892996
50	21.06618906116059	23.055881120472726	31.49239667065853	24.385533147708152
51	21.640081425499282	22.857847839820643	29.70903359333621	25.79303714134386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	288.0
1	578.5
2	869.0
3	7142.5
4	13416.0
5	8539.5
6	3663.0
7	3563.5
8	3464.0
9	3416.0
10	3368.0
11	3228.5
12	3089.0
13	3005.5
14	2922.0
15	2647.0
16	2372.0
17	2239.5
18	2107.0
19	2025.0
20	1943.0
21	1902.0
22	1861.0
23	2021.5
24	2182.0
25	2473.5
26	3156.0
27	3547.0
28	4250.0
29	4953.0
30	5598.0
31	6243.0
32	6900.0
33	7557.0
34	8654.5
35	9752.0
36	10306.0
37	10860.0
38	11653.5
39	12447.0
40	14131.5
41	15816.0
42	17620.5
43	19425.0
44	21994.0
45	24563.0
46	32069.5
47	39576.0
48	39019.0
49	38462.0
50	38755.0
51	39048.0
52	36145.0
53	33242.0
54	31101.0
55	28960.0
56	27055.5
57	25151.0
58	23784.5
59	22418.0
60	21667.0
61	20916.0
62	19145.0
63	17374.0
64	15198.5
65	13023.0
66	11206.5
67	9390.0
68	8138.5
69	6887.0
70	6031.5
71	5176.0
72	4560.0
73	3944.0
74	3490.5
75	2491.5
76	1946.0
77	1476.0
78	1006.0
79	823.0
80	640.0
81	469.5
82	299.0
83	213.5
84	128.0
85	73.0
86	18.0
87	10.5
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470123.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.37688833675949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.48051182175146	26.002385967942704
2	7.950717933790114	5.784447569491082
3	3.6571252332660062	3.9910450873218912
4	2.58170961403664	3.756582493909972
5	2.021790092750939	3.677321622218376
6	1.7705409906578495	3.864406314768968
7	1.4926216316587395	3.8007851296717208
8	1.3462531603163377	3.91780007086696
9	1.202957761371299	3.938387414331773
>10	6.413457736727597	33.80475895402493
>50	0.047624542267969854	1.2088530089728082
>100	0.029397865597512233	2.2816779723499487
>500	0.002351829247800979	0.5800441189551051
>1k	0.002351829247800979	1.5792941645886787
>5k	5.879573119502448E-4	1.8122101105850317
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8473	1.8022942931956105	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	2438	0.5185876887537942	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	2155	0.4583906764825375	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	1681	0.3575659986854504	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC	1110	0.23610842268938131	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	973	0.20696711286195316	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	658	0.13996337128793954	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	580	0.12337196861246952	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	501	0.10656785564628832	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT	488	0.10380262186704331	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTG	478	0.10167551895993177	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	476	0.10125009837850946	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	474	0.10082467779708715	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.254205814223086E-4	0.0	0.0	0.10550430419273255	0.0
2	4.254205814223086E-4	0.0	0.0	0.5249689974751288	0.0
3	4.254205814223086E-4	0.0	0.0	0.7951110666782948	0.0
4	4.254205814223086E-4	0.0	0.0	1.2390374433924738	0.0
5	4.254205814223086E-4	0.0	0.0	2.6303754549341343	0.0
6	4.254205814223086E-4	0.0	0.0	3.514399423129692	0.0
7	4.254205814223086E-4	0.0	0.0	4.347798342135994	0.0
8	4.254205814223086E-4	0.0	0.0	5.436662320286393	0.0
9	4.254205814223086E-4	0.0	0.0	6.011192815497221	0.0
10	4.254205814223086E-4	0.0	0.0	7.423376435528574	0.0
11	4.254205814223086E-4	0.0	0.0	8.647736868861978	0.0
12	4.254205814223086E-4	0.0	0.0	9.835723842483775	0.0
13	4.254205814223086E-4	0.0	0.0	10.248807227044837	0.0
14	4.254205814223086E-4	0.0	0.0	10.429398263858607	0.0
15	4.254205814223086E-4	0.0	0.0	10.751016223413872	0.0
16	4.254205814223086E-4	0.0	0.0	11.2168517600713	0.0
17	4.254205814223086E-4	0.0	0.0	11.719911597603181	0.0
18	4.254205814223086E-4	0.0	0.0	12.28167947537134	0.0
19	4.254205814223086E-4	0.0	0.0	12.689657812955334	0.0
20	4.254205814223086E-4	0.0	0.0	13.045734839605805	0.0
21	4.254205814223086E-4	0.0	0.0	13.426698970269483	0.0
22	4.254205814223086E-4	0.0	0.0	13.82978497116712	0.0
23	4.254205814223086E-4	0.0	0.0	14.229893027994802	0.0
24	4.254205814223086E-4	0.0	0.0	14.543640706793754	0.0
25	4.254205814223086E-4	0.0	0.0	14.839095300591547	0.0
26	4.254205814223086E-4	0.0	0.0	15.114555127062491	0.0
27	4.254205814223086E-4	0.0	0.0	15.388100560917037	0.0
28	4.254205814223086E-4	0.0	0.0	15.669516275527894	0.0
29	4.254205814223086E-4	0.0	0.0	15.952846382755151	0.0
30	4.254205814223086E-4	0.0	0.0	16.296586212544376	0.0
31	4.254205814223086E-4	0.0	0.0	16.594167909249283	0.0
32	4.254205814223086E-4	0.0	0.0	16.913871476188145	0.0
33	4.254205814223086E-4	0.0	0.0	17.20932606998594	0.0
34	4.254205814223086E-4	0.0	0.0	17.49478328012031	0.0
35	4.254205814223086E-4	0.0	0.0	17.785558247522456	0.0
36	4.254205814223086E-4	0.0	0.0	18.06016723283056	0.0
37	4.254205814223086E-4	0.0	0.0	18.356472667791195	0.0
38	4.254205814223086E-4	0.0	0.0	18.68574819781206	0.0
39	4.254205814223086E-4	0.0	0.0	19.02097961597284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGTCG	20	7.029027E-4	45.000004	1
TAGTGCG	20	7.029027E-4	45.000004	1
ATTTCGT	20	7.029027E-4	45.000004	12
CGTTTTT	3745	0.0	42.47663	1
GTTAATC	45	1.924127E-8	40.0	40
TAATGCG	35	6.240414E-6	38.571426	1
ATACTAT	65	9.094947E-12	38.076927	45
ATTGGGC	160	0.0	37.968754	4
AGATCGG	30	1.13889575E-4	37.499996	2
CGAGGGT	60	1.546141E-10	37.499996	4
CATATGC	145	0.0	37.241383	33
CGGGTAC	80	0.0	36.562504	6
GGTACCT	160	0.0	36.562504	8
GTTTTTT	4515	0.0	36.1794	2
TATGGGC	175	0.0	36.000004	4
TTGATCC	150	0.0	36.0	17
CGGCGAA	25	0.002105845	36.0	31
CGAAAGG	75	1.8189894E-12	36.0	2
TAGCGCG	25	0.002105845	36.0	1
ACCGAGT	25	0.002105845	36.0	28
>>END_MODULE