FastQCFastQC Report
Sat 18 Jun 2016
SRR3551986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462902
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46731.0095009310826049No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC9520.20565908118781082No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC9490.20501099584793325No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG7760.1676380745816609No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC7740.16720601768840923No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC6110.13199338088839538No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT5640.12184004389698036TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT406.7993824E-945.00000428
AACCCGA207.028915E-445.00000424
CTAAGCG207.028915E-445.0000041
GCGCATA207.028915E-445.0000041
GCCATAG207.028915E-445.0000041
TCGACGT207.028915E-445.00000426
CTCACGT351.209828E-745.045
CGAATAT253.8871833E-545.014
CGTTTTT20250.041.01
GTAGTAG403.45226E-739.3750041
AACGGGA2200.038.8636364
TAGGGTA1050.038.571435
TAGCCGT356.240207E-638.57142644
TAGCATA1400.038.57142630
TAAGCGG700.038.5714262
ATGGGCG1300.038.0769275
CCCGATC659.094947E-1238.07692741
AAACCGG301.1388688E-437.4999962
CTACCCA601.546141E-1037.49999616
GCTACGA301.1388688E-437.49999610