##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462902
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.350125944584384	33.0	31.0	34.0	30.0	34.0
2	32.443158595123805	34.0	31.0	34.0	31.0	34.0
3	32.43633857706383	34.0	31.0	34.0	30.0	34.0
4	35.979544266389	37.0	35.0	37.0	35.0	37.0
5	36.04446297488453	37.0	35.0	37.0	35.0	37.0
6	36.032384824433684	37.0	35.0	37.0	35.0	37.0
7	36.21858190286497	37.0	37.0	37.0	35.0	37.0
8	36.07008826922329	37.0	37.0	37.0	35.0	37.0
9	37.98604456234797	39.0	38.0	39.0	35.0	39.0
10	37.66563765116591	39.0	37.0	39.0	35.0	39.0
11	37.62092624356775	39.0	37.0	39.0	35.0	39.0
12	37.56270873748655	39.0	37.0	39.0	35.0	39.0
13	37.57592103728219	39.0	37.0	39.0	35.0	39.0
14	38.86197942545074	40.0	38.0	41.0	35.0	41.0
15	38.921091289300975	40.0	38.0	41.0	35.0	41.0
16	38.916351625182	40.0	38.0	41.0	35.0	41.0
17	38.86370117217035	40.0	38.0	41.0	35.0	41.0
18	38.74502162444751	40.0	38.0	41.0	35.0	41.0
19	38.65846766702239	40.0	38.0	41.0	35.0	41.0
20	38.56812025007453	40.0	38.0	41.0	35.0	41.0
21	38.46980786429957	40.0	37.0	41.0	35.0	41.0
22	38.48226838510095	40.0	37.0	41.0	35.0	41.0
23	38.44845777291954	40.0	37.0	41.0	35.0	41.0
24	38.38365139921625	40.0	37.0	41.0	34.0	41.0
25	38.21999040833697	40.0	37.0	41.0	34.0	41.0
26	38.237214356386446	40.0	37.0	41.0	34.0	41.0
27	38.24285701941232	40.0	37.0	41.0	34.0	41.0
28	38.175948688923356	40.0	37.0	41.0	34.0	41.0
29	38.124527437773004	40.0	37.0	41.0	34.0	41.0
30	37.95269625104234	40.0	36.0	41.0	34.0	41.0
31	37.89027267110533	40.0	36.0	41.0	34.0	41.0
32	37.75922333452869	40.0	36.0	41.0	33.0	41.0
33	37.62036672989099	40.0	36.0	41.0	33.0	41.0
34	37.480544478096874	40.0	36.0	41.0	33.0	41.0
35	37.37176335379843	40.0	36.0	41.0	33.0	41.0
36	37.28316576726824	40.0	36.0	41.0	33.0	41.0
37	37.25639336187789	40.0	36.0	41.0	33.0	41.0
38	37.133067042268124	40.0	35.0	41.0	32.0	41.0
39	37.090863724935296	40.0	35.0	41.0	32.0	41.0
40	36.99237635611858	40.0	35.0	41.0	32.0	41.0
41	36.91819434783172	40.0	35.0	41.0	32.0	41.0
42	36.82434510976405	39.0	35.0	41.0	32.0	41.0
43	36.69481013259826	39.0	35.0	41.0	31.0	41.0
44	36.71810664028239	39.0	35.0	41.0	31.0	41.0
45	36.67949371573249	39.0	35.0	41.0	31.0	41.0
46	36.58816120906801	39.0	35.0	41.0	31.0	41.0
47	36.53700567290701	39.0	35.0	41.0	31.0	41.0
48	36.494925491788756	39.0	35.0	41.0	31.0	41.0
49	36.44749212576312	39.0	35.0	41.0	31.0	41.0
50	36.36817512129997	39.0	35.0	41.0	31.0	41.0
51	35.535247201351474	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	4.0
11	4.0
12	12.0
13	10.0
14	10.0
15	21.0
16	25.0
17	57.0
18	105.0
19	174.0
20	293.0
21	505.0
22	730.0
23	1131.0
24	1749.0
25	2719.0
26	3849.0
27	4867.0
28	5012.0
29	5117.0
30	5450.0
31	6455.0
32	8262.0
33	11581.0
34	22460.0
35	31827.0
36	32037.0
37	47165.0
38	88547.0
39	182507.0
40	208.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65417734207241	20.23776090835641	24.379242258620614	14.728819490950567
2	34.302292925932484	24.3055765583212	24.608880497383893	16.783250018362416
3	31.788585920994077	23.616445813584733	28.132952547191415	16.46201571822978
4	28.209426617296966	25.203822839391492	28.18371923214849	18.403031311163055
5	25.423955826503235	29.04567273418564	25.66093903245179	19.869432406859335
6	26.0871631576446	33.55526655750029	25.414018517958443	14.943551766896665
7	78.188039801081	5.906865816090663	11.452316040976276	4.4527783418520555
8	79.02709428777581	5.686948857425546	10.648474191081482	4.637482663717158
9	72.4287214140358	7.9962929518559	12.953929773472572	6.621055860635729
10	36.70863379289785	29.36690703431828	19.10469170580382	14.819767466980052
11	28.552263762092196	24.039645540524777	28.282876289149755	19.125214408233276
12	26.14073821240781	21.101442637966567	31.709519509529017	21.04829964009661
13	25.023439086458904	21.82945850309569	32.880177661794505	20.2669247486509
14	21.523994279566733	25.493949043210012	31.134451784611	21.84760489261226
15	20.202332243109772	24.73936167914591	34.415923888857684	20.642382188886632
16	23.0003326838078	23.002060911380813	32.30921447736238	21.688391927449008
17	22.327836129461527	23.46933044143253	30.52676376425248	23.67606966485347
18	22.938332519626183	23.240556316455752	31.27919084385032	22.541920320067746
19	23.40776233414416	25.080902653261383	29.412489036556334	22.098845976038124
20	24.483800026787527	24.93097891130304	29.674315513866866	20.910905548042567
21	23.78840445709891	25.32155834280258	29.681444452605522	21.20859274749299
22	22.654903197653066	23.47084264055891	29.18457902536606	24.689675136421965
23	21.946545921166898	25.628966822351167	29.69246190338344	22.732025353098496
24	22.768534160578266	24.461549096785067	30.38915364375181	22.380763098884863
25	22.961663591861775	25.409913977472552	28.499552821115486	23.128869609550186
26	21.37990330566729	26.615352709644807	28.963365896021187	23.04137808866672
27	22.65058262872055	25.54665998418672	29.552907526863137	22.249849860229595
28	21.173380110692978	26.04050101317342	30.424366280551823	22.361752595581784
29	22.65727951056595	25.690534929639536	28.80544910153769	22.84673645825682
30	22.9108969069047	25.465865345148647	29.7857861923258	21.83745155562084
31	23.299748110831235	26.846503147534467	27.210943136992277	22.642805604642017
32	24.07853066091743	27.184371638057296	27.02494264444742	21.71215505657785
33	23.583609489697604	26.50755451477851	26.967047020751693	22.941788974772198
34	23.03014460944217	27.009604624736987	27.422651014685613	22.53759975113523
35	22.418136020151135	25.91218011587766	27.691390402288174	23.978293461683034
36	22.302992858099554	28.071600468349672	27.039848607264606	22.58555806628617
37	23.65511490553076	26.491136352834943	27.69787125568695	22.15587748594735
38	22.453348656951146	27.509494450229205	26.109198059200434	23.92795883361921
39	23.915645212161536	25.316373660083556	26.6211854777037	24.146795650051196
40	23.4423268856043	24.5779884295164	29.294753533145247	22.684931151734062
41	21.120453141269643	25.270791657845503	28.252200249728883	25.356554951155967
42	22.204267857991542	25.731148277605193	27.683613378209643	24.380970486193622
43	22.05499220137308	25.48617201913148	28.53109297432286	23.927742805172585
44	23.19540637111095	25.6751969099291	27.943927656393797	23.18546906256616
45	22.949782027297356	24.894254075376647	28.09298728456563	24.062976612760366
46	23.197350627130582	25.606067807008827	27.878903093959412	23.31767847190118
47	21.532203360538517	26.254369175333007	28.994041935442063	23.219385528686416
48	22.093013207979226	24.394364249884426	28.61815243831308	24.89447010382327
49	22.00984225602827	24.54882458922191	29.235777767216387	24.20555538753343
50	21.474523765289412	23.793589139817932	29.604538325606715	25.12734876928594
51	21.344690668867276	24.161485584421758	28.191280227780396	26.30254351893057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	575.5
2	893.0
3	4110.5
4	7328.0
5	4674.5
6	2021.0
7	2002.0
8	1983.0
9	1946.5
10	1910.0
11	1817.5
12	1725.0
13	1690.0
14	1655.0
15	1566.0
16	1477.0
17	1496.5
18	1516.0
19	1584.0
20	1652.0
21	1656.5
22	1661.0
23	1865.5
24	2070.0
25	2644.0
26	3676.0
27	4134.0
28	4995.0
29	5856.0
30	6480.5
31	7105.0
32	7864.5
33	8624.0
34	9601.5
35	10579.0
36	11627.5
37	12676.0
38	13752.5
39	14829.0
40	16618.5
41	18408.0
42	20871.5
43	23335.0
44	25542.5
45	27750.0
46	32410.0
47	37070.0
48	39610.5
49	42151.0
50	41163.5
51	40176.0
52	35791.0
53	31406.0
54	28635.0
55	25864.0
56	24251.0
57	22638.0
58	21456.5
59	20275.0
60	19891.5
61	19508.0
62	17948.5
63	16389.0
64	14753.0
65	13117.0
66	11093.0
67	9069.0
68	7777.5
69	6486.0
70	5748.0
71	5010.0
72	4700.0
73	4390.0
74	3655.5
75	2282.5
76	1644.0
77	1300.5
78	957.0
79	763.0
80	569.0
81	458.0
82	347.0
83	263.5
84	180.0
85	108.0
86	36.0
87	24.0
88	12.0
89	10.5
90	9.0
91	8.5
92	8.0
93	7.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	462902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.15817325660655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.64602018581327	24.472971706533407
2	8.234955355182727	5.625820635655031
3	3.4115469293856906	3.4959663326100148
4	2.1248323080622544	2.9032156048010274
5	1.6263769206717311	2.7777032318425623
6	1.4126371122821062	2.8951861938026733
7	1.2291440527310702	2.938972085736081
8	1.1460397618024274	3.131729979408594
9	1.0358380600641715	3.1844102329313277
>10	8.03683198640975	43.046139551274024
>50	0.057720111331932174	1.3874959625450904
>100	0.03361720769881764	2.1256594141989194
>500	0.0038057216262812423	1.0022730047991237
>1k	6.342869377135405E-4	1.0124560638621498
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4673	1.0095009310826049	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	952	0.20565908118781082	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	949	0.20501099584793325	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	776	0.1676380745816609	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	774	0.16720601768840923	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	611	0.13199338088839538	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	564	0.12184004389698036	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1261606128294974	0.0
2	0.0	0.0	0.0	0.5670746723928607	0.0
3	0.0	0.0	0.0	0.8241485238776242	0.0
4	0.0	0.0	0.0	1.241083425865518	0.0
5	0.0	0.0	0.0	2.4067729238586137	0.0
6	0.0	0.0	0.0	3.1952767540429723	0.0
7	0.0	0.0	0.0	3.9496480896604464	0.0
8	0.0	0.0	0.0	5.116201701440046	0.0
9	0.0	0.0	0.0	5.685436658299165	0.0
10	0.0	0.0	0.0	6.864087863089812	0.0
11	0.0	0.0	0.0	8.166955424690324	0.0
12	0.0	0.0	0.0	9.257899080150874	0.0
13	0.0	0.0	0.0	9.639621345338755	0.0
14	0.0	0.0	0.0	9.799266367395258	0.0
15	0.0	0.0	0.0	10.092849026359792	0.0
16	0.0	0.0	0.0	10.651282560887617	0.0
17	0.0	0.0	0.0	11.286190165520996	0.0
18	0.0	0.0	0.0	12.00923737637772	0.0
19	0.0	0.0	0.0	12.446911009241697	0.0
20	0.0	0.0	0.0	12.869030593948612	0.0
21	0.0	0.0	0.0	13.35963119623592	0.0
22	0.0	0.0	0.0	13.853256196775991	0.0
23	0.0	0.0	0.0	14.358330704987234	0.0
24	0.0	0.0	0.0	14.74955822182665	0.0
25	0.0	0.0	0.0	15.116374524197346	0.0
26	0.0	0.0	0.0	15.457267412972941	0.0
27	0.0	0.0	0.0	15.798592358641786	0.0
28	0.0	0.0	0.0	16.155255324021066	0.0
29	0.0	0.0	0.0	16.54151418658809	0.0
30	0.0	0.0	0.0	16.96709022644102	0.0
31	0.0	0.0	0.0	17.347948377842393	0.0
32	0.0	0.0	0.0	17.71692496467935	0.0
33	0.0	0.0	0.0	18.087629779089312	0.0
34	0.0	0.0	0.0	18.461358991752032	0.0
35	0.0	0.0	0.0	18.827743237229477	0.0
36	0.0	0.0	0.0	19.202768620571955	0.0
37	0.0	0.0	0.0	19.570016979835906	0.0
38	0.0	0.0	0.0	19.93575313997347	0.0
39	0.0	0.0	0.0	20.306890011276685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	40	6.7993824E-9	45.000004	28
AACCCGA	20	7.028915E-4	45.000004	24
CTAAGCG	20	7.028915E-4	45.000004	1
GCGCATA	20	7.028915E-4	45.000004	1
GCCATAG	20	7.028915E-4	45.000004	1
TCGACGT	20	7.028915E-4	45.000004	26
CTCACGT	35	1.209828E-7	45.0	45
CGAATAT	25	3.8871833E-5	45.0	14
CGTTTTT	2025	0.0	41.0	1
GTAGTAG	40	3.45226E-7	39.375004	1
AACGGGA	220	0.0	38.863636	4
TAGGGTA	105	0.0	38.57143	5
TAGCCGT	35	6.240207E-6	38.571426	44
TAGCATA	140	0.0	38.571426	30
TAAGCGG	70	0.0	38.571426	2
ATGGGCG	130	0.0	38.076927	5
CCCGATC	65	9.094947E-12	38.076927	41
AAACCGG	30	1.1388688E-4	37.499996	2
CTACCCA	60	1.546141E-10	37.499996	16
GCTACGA	30	1.1388688E-4	37.499996	10
>>END_MODULE