FastQCFastQC Report
Sat 18 Jun 2016
SRR3551985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707759
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60830.859473351804781No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC15740.22239208544151326No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT14440.20402425119284956Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC12990.18353705145395538No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC12320.17407055226425944No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8190.11571735576658157No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC7750.1095005503285723No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG207.031447E-445.01
CTTACGA207.031447E-445.024
AATATAC1900.042.6315816
CGAATAT1900.041.4473714
CGTTTTT25900.040.5694961
TATTGCG501.0804797E-940.5000041
CTACGAA2100.039.64285711
GCAAACG403.455716E-739.3751
CACGAAT403.455716E-739.37529
TACACGG403.455716E-739.3752
GCTACGA2100.038.5714310
TAACGCC356.244889E-638.57142612
TCGTAAG356.244889E-638.5714261
CGTAAAT356.244889E-638.57142616
TACGAAT2050.038.41463512
TAGGGAC3700.038.310815
AGGGTAC2150.037.674426
AAGCTAC2100.037.58
AAATCGT301.1394788E-437.49999612
ACCGACC301.1394788E-437.49999631