FastQCFastQC Report
Sat 18 Jun 2016
SRR3551983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321819
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.5459590639458827No Hit
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC9220.2864964467604461Illumina PCR Primer Index 9 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG6570.20415202334231353No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC5650.17556452540092413RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC5340.165931781529369No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC5280.16406737948971317No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5190.1612707764302294No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT4320.13423694685521986RNA PCR Primer, Index 17 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC3940.12242906727073294RNA PCR Primer, Index 17 (95% over 24bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3730.11590366013193752No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3300.10254211218107072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA650.045.00000410
ACAACGA207.025709E-445.013
TTGATCG207.025709E-445.01
TCGTTTG253.8845254E-545.01
ATAGCGG502.1827873E-1145.02
CAAGGTA207.025709E-445.04
CGTAAGG207.025709E-445.02
GTATAAG253.8845254E-545.01
TCATACG207.025709E-445.031
AATAGCG253.8845254E-545.01
CACAACG207.025709E-445.012
TATCGTG207.025709E-445.01
TAAGACG207.025709E-445.01
TACACGC950.045.035
CTACCGG302.1606138E-644.9999962
ACACGCG1000.042.7536
TTACACG1000.042.7534
TACGAAT700.041.78571312
CGAATAT700.041.78571314
GACCTAT700.041.78571333