Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551983_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 321819 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1757 | 0.5459590639458827 | No Hit |
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC | 922 | 0.2864964467604461 | Illumina PCR Primer Index 9 (95% over 23bp) |
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG | 657 | 0.20415202334231353 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC | 565 | 0.17556452540092413 | RNA PCR Primer, Index 17 (95% over 21bp) |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC | 534 | 0.165931781529369 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC | 528 | 0.16406737948971317 | No Hit |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 519 | 0.1612707764302294 | No Hit |
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT | 432 | 0.13423694685521986 | RNA PCR Primer, Index 17 (96% over 25bp) |
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC | 394 | 0.12242906727073294 | RNA PCR Primer, Index 17 (95% over 24bp) |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 373 | 0.11590366013193752 | No Hit |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 330 | 0.10254211218107072 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCTACGA | 65 | 0.0 | 45.000004 | 10 |
ACAACGA | 20 | 7.025709E-4 | 45.0 | 13 |
TTGATCG | 20 | 7.025709E-4 | 45.0 | 1 |
TCGTTTG | 25 | 3.8845254E-5 | 45.0 | 1 |
ATAGCGG | 50 | 2.1827873E-11 | 45.0 | 2 |
CAAGGTA | 20 | 7.025709E-4 | 45.0 | 4 |
CGTAAGG | 20 | 7.025709E-4 | 45.0 | 2 |
GTATAAG | 25 | 3.8845254E-5 | 45.0 | 1 |
TCATACG | 20 | 7.025709E-4 | 45.0 | 31 |
AATAGCG | 25 | 3.8845254E-5 | 45.0 | 1 |
CACAACG | 20 | 7.025709E-4 | 45.0 | 12 |
TATCGTG | 20 | 7.025709E-4 | 45.0 | 1 |
TAAGACG | 20 | 7.025709E-4 | 45.0 | 1 |
TACACGC | 95 | 0.0 | 45.0 | 35 |
CTACCGG | 30 | 2.1606138E-6 | 44.999996 | 2 |
ACACGCG | 100 | 0.0 | 42.75 | 36 |
TTACACG | 100 | 0.0 | 42.75 | 34 |
TACGAAT | 70 | 0.0 | 41.785713 | 12 |
CGAATAT | 70 | 0.0 | 41.785713 | 14 |
GACCTAT | 70 | 0.0 | 41.785713 | 33 |