##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551983_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321819
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28559221177121	33.0	31.0	34.0	30.0	34.0
2	32.37206317837045	34.0	31.0	34.0	30.0	34.0
3	32.37201967565619	34.0	31.0	34.0	30.0	34.0
4	35.924780699710084	37.0	35.0	37.0	35.0	37.0
5	35.9896898567207	37.0	35.0	37.0	35.0	37.0
6	35.97052691108978	37.0	35.0	37.0	35.0	37.0
7	36.13954427799477	37.0	36.0	37.0	35.0	37.0
8	35.97785090376889	37.0	35.0	37.0	35.0	37.0
9	37.88616893346882	39.0	38.0	39.0	35.0	39.0
10	37.570246007849136	39.0	37.0	39.0	35.0	39.0
11	37.52822549321202	39.0	37.0	39.0	35.0	39.0
12	37.41883170353522	39.0	37.0	39.0	35.0	39.0
13	37.38990861322669	39.0	37.0	39.0	35.0	39.0
14	38.651658230247435	40.0	38.0	41.0	35.0	41.0
15	38.72409646416153	40.0	38.0	41.0	35.0	41.0
16	38.7259142561502	40.0	38.0	41.0	35.0	41.0
17	38.672116935295925	40.0	38.0	41.0	35.0	41.0
18	38.58858550924588	40.0	38.0	41.0	35.0	41.0
19	38.49762754840454	40.0	37.0	41.0	34.0	41.0
20	38.41178737116205	40.0	37.0	41.0	34.0	41.0
21	38.31121841780628	40.0	37.0	41.0	34.0	41.0
22	38.326655666694634	40.0	37.0	41.0	34.0	41.0
23	38.28960999816667	40.0	37.0	41.0	34.0	41.0
24	38.21917599644521	40.0	37.0	41.0	34.0	41.0
25	38.04523039348205	40.0	36.0	41.0	34.0	41.0
26	38.092648352023964	40.0	36.0	41.0	34.0	41.0
27	38.08141532973504	40.0	36.0	41.0	34.0	41.0
28	37.9962059418493	40.0	36.0	41.0	34.0	41.0
29	37.943076698392574	40.0	36.0	41.0	34.0	41.0
30	37.79413894145467	40.0	36.0	41.0	33.0	41.0
31	37.74273737722136	40.0	36.0	41.0	33.0	41.0
32	37.62104785609302	40.0	36.0	41.0	33.0	41.0
33	37.524996970346685	40.0	36.0	41.0	33.0	41.0
34	37.410239917469134	40.0	35.0	41.0	33.0	41.0
35	37.29790658724314	40.0	35.0	41.0	33.0	41.0
36	37.21411103757081	40.0	35.0	41.0	33.0	41.0
37	37.16647245812087	40.0	35.0	41.0	32.0	41.0
38	37.03437646627452	40.0	35.0	41.0	32.0	41.0
39	36.969554314692424	39.0	35.0	41.0	32.0	41.0
40	36.85472579307002	39.0	35.0	41.0	32.0	41.0
41	36.75586587491727	39.0	35.0	41.0	31.0	41.0
42	36.645760505128656	39.0	35.0	41.0	31.0	41.0
43	36.51328852553765	39.0	35.0	41.0	31.0	41.0
44	36.532327177699266	39.0	35.0	41.0	31.0	41.0
45	36.494513996998315	39.0	35.0	41.0	31.0	41.0
46	36.40823568527651	39.0	35.0	41.0	31.0	41.0
47	36.33062373570237	39.0	35.0	41.0	31.0	41.0
48	36.25854284551254	38.0	35.0	41.0	31.0	41.0
49	36.18626930044528	38.0	35.0	41.0	31.0	41.0
50	36.05686115487277	38.0	35.0	40.0	31.0	41.0
51	35.1439100861043	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	6.0
11	4.0
12	10.0
13	12.0
14	11.0
15	21.0
16	32.0
17	46.0
18	82.0
19	135.0
20	217.0
21	415.0
22	638.0
23	902.0
24	1298.0
25	1842.0
26	2524.0
27	3003.0
28	3217.0
29	3481.0
30	4139.0
31	5068.0
32	6370.0
33	9065.0
34	17691.0
35	25429.0
36	23169.0
37	35173.0
38	61826.0
39	115867.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94022727060863	19.978310789605334	23.264008650825463	12.817453288960564
2	33.379943384324726	24.821095087611358	24.695558683607864	17.103402844456046
3	32.70036884087018	23.904430751447244	26.48724904371712	16.90795136396546
4	29.12848526656288	24.97739412526917	26.596316563036986	19.29780404513096
5	25.357732141358962	29.656111043785483	24.51098288168194	20.475173933173615
6	26.451514671290383	35.12347002507621	24.20459947983183	14.220415823801577
7	77.68528272103264	7.195038204705129	10.525481714876996	4.5941973593852445
8	77.78502823015423	6.959812814035218	10.101951718201846	5.153207237608718
9	71.09524297819581	9.005683318884218	11.98313337621458	7.915940326705384
10	39.20868562763541	25.636460246287506	18.887635596406678	16.267218529670405
11	31.6190156578698	24.66852485403286	24.35965558279654	19.352803905300807
12	28.936141122805054	21.866017854756866	29.634359686656165	19.563481335781916
13	24.896914103890698	24.054204381966258	30.238115213831378	20.810766300311666
14	21.42601897339809	27.527896115518352	28.609249298518733	22.436835612564828
15	20.92231968901774	25.264201305702894	33.057091097790995	20.75638790748837
16	22.74352974808821	23.44796298540484	32.04844959433719	21.76005767216976
17	22.110565255625055	23.981492702419683	28.765548336176543	25.142393705778716
18	23.057992225443495	24.405644166441384	29.561026539763034	22.975337068352086
19	25.493212022907286	25.965527206286765	26.472023093726598	22.06923767707935
20	27.34145591155277	24.15830016251371	27.76405370720809	20.736190218725433
21	25.42733648417278	25.95838033180142	26.65722036299908	21.957062821026728
22	23.505137981287618	24.362141452182748	26.78431043536895	25.34841013116068
23	24.343808165459464	26.21660001429375	26.8094798629043	22.63011195734248
24	23.984600039152443	24.226661570634427	28.534983950605774	23.25375443960736
25	22.822145367427034	26.460525947815388	26.682389790534433	24.034938894223153
26	23.021636385670206	26.90922537202589	26.827502415954307	23.241635826349594
27	24.75925908662944	26.1771368377877	26.861061652668116	22.202542422914746
28	22.295141057550982	26.95459248832418	28.277696469133275	22.472569984991566
29	24.03866769830246	26.264763733651524	26.763802012932736	22.93276655511328
30	24.91835472734674	25.60414394426681	26.198577461243744	23.278923867142712
31	25.832533194124647	26.81258719963706	23.2428787610427	24.112000845195592
32	25.54137574226506	27.84950546735898	24.818298484551875	21.79082030582408
33	25.84496254105569	27.533489321637315	23.6269455812118	22.994602556095195
34	24.45007908171985	27.451144898219187	25.22380592817702	22.87497009188395
35	25.028354447686436	25.20951217920632	25.590471662642667	24.17166171046458
36	24.561632470425923	28.272724730360853	24.035249627896427	23.130393171316797
37	24.080616744194717	28.941734328924024	25.324483638318434	21.653165288562825
38	24.092424623779205	27.5853818450744	25.190868158809764	23.131325372336626
39	25.14332590679854	26.045696493991965	26.034199348080755	22.77677825112874
40	23.687538647500613	25.098580257846802	28.812469120841218	22.401411973811367
41	21.596301026353323	27.094733374971646	27.648771514422705	23.66019408425233
42	23.53341474555573	26.590723356918016	26.747022394575833	23.128839502950417
43	24.230701108387013	25.779087002321184	25.96024473384107	24.029967155450734
44	24.26426034510082	25.477986072916764	26.290865362206706	23.96688821977571
45	23.924317706536904	25.08242210683645	25.632109974861645	25.361150211765
46	23.25530810797374	27.236738663658766	26.378802991743804	23.12915023662369
47	23.60705862612214	26.116543771498886	27.631059695045973	22.645337907333005
48	22.851975800061524	25.422986212746917	27.536596658370076	24.18844132882148
49	22.692258691997676	26.33902908156448	27.887104241825373	23.081607984612468
50	21.76005767216976	25.160726992501996	28.169561150833232	24.90965418449501
51	21.81661120070599	25.437901429064162	26.37320978562484	26.37227758460501
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	213.0
1	523.5
2	834.0
3	1876.5
4	2919.0
5	1958.5
6	998.0
7	1054.5
8	1111.0
9	1226.0
10	1341.0
11	1220.0
12	1099.0
13	1096.5
14	1094.0
15	1071.0
16	1048.0
17	1029.0
18	1010.0
19	1005.5
20	1001.0
21	998.0
22	995.0
23	1174.0
24	1353.0
25	1696.5
26	2321.0
27	2602.0
28	3236.0
29	3870.0
30	4335.5
31	4801.0
32	5476.5
33	6152.0
34	6719.5
35	7287.0
36	7874.0
37	8461.0
38	8651.0
39	8841.0
40	10485.0
41	12129.0
42	13319.5
43	14510.0
44	16523.0
45	18536.0
46	21463.0
47	24390.0
48	26138.5
49	27887.0
50	26788.5
51	25690.0
52	23608.0
53	21526.0
54	19565.0
55	17604.0
56	16987.5
57	16371.0
58	16242.5
59	16114.0
60	16114.5
61	16115.0
62	15658.0
63	15201.0
64	13290.5
65	11380.0
66	9437.0
67	7494.0
68	6435.5
69	5377.0
70	4852.5
71	4328.0
72	3737.5
73	3147.0
74	2612.5
75	1717.5
76	1357.0
77	1120.5
78	884.0
79	595.0
80	306.0
81	223.5
82	141.0
83	141.5
84	142.0
85	82.5
86	23.0
87	16.5
88	10.0
89	7.0
90	4.0
91	4.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	321819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.050108792456484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.3515721330576	22.547755405848456
2	8.649372348326963	5.54426649500688
3	3.300909994142662	3.173835732791376
4	2.1758592737608344	2.789461057644404
5	1.601198128667333	2.565928711103288
6	1.3884155163897853	2.6699321009656356
7	1.2066208300979429	2.707063021311825
8	1.1227165078474468	2.878654897567799
9	1.0547941331194501	3.042564004811088
>10	9.041663320269253	46.856894136368844
>50	0.0659246331324086	1.429403224203677
>100	0.03396117464396806	2.039260590857205
>500	0.005993148466582599	1.192503249755587
>1k	9.988580777637666E-4	0.5624773717639104
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1757	0.5459590639458827	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	922	0.2864964467604461	Illumina PCR Primer Index 9 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCG	657	0.20415202334231353	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTC	565	0.17556452540092413	RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC	534	0.165931781529369	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCC	528	0.16406737948971317	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	519	0.1612707764302294	No Hit
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	432	0.13423694685521986	RNA PCR Primer, Index 17 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	394	0.12242906727073294	RNA PCR Primer, Index 17 (95% over 24bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	373	0.11590366013193752	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	330	0.10254211218107072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.107336732759719E-4	0.0	0.0	0.13858721828108347	0.0
2	3.107336732759719E-4	0.0	0.0	0.7112693781286997	0.0
3	3.107336732759719E-4	0.0	0.0	0.9694890606210324	0.0
4	3.107336732759719E-4	0.0	0.0	1.3227932471358124	0.0
5	3.107336732759719E-4	0.0	0.0	2.4454740086818987	0.0
6	3.107336732759719E-4	0.0	0.0	3.042082661371765	0.0
7	3.107336732759719E-4	0.0	0.0	3.60637501204093	0.0
8	3.107336732759719E-4	0.0	0.0	4.501909458422281	0.0
9	3.107336732759719E-4	0.0	0.0	4.859253182689648	0.0
10	3.107336732759719E-4	0.0	0.0	5.849250665746895	0.0
11	3.107336732759719E-4	0.0	0.0	6.9458297987378	0.0
12	3.107336732759719E-4	0.0	0.0	7.840742777772599	0.0
13	3.107336732759719E-4	0.0	0.0	8.186900089802032	0.0
14	3.107336732759719E-4	0.0	0.0	8.351588936638297	0.0
15	3.107336732759719E-4	0.0	0.0	8.654865001755645	0.0
16	3.107336732759719E-4	0.0	0.0	9.208281673860151	0.0
17	3.107336732759719E-4	0.0	0.0	9.856472116313828	3.107336732759719E-4
18	3.107336732759719E-4	0.0	0.0	10.548165273026141	3.107336732759719E-4
19	3.107336732759719E-4	0.0	0.0	10.9741811390875	3.107336732759719E-4
20	3.107336732759719E-4	0.0	0.0	11.352344019464358	3.107336732759719E-4
21	3.107336732759719E-4	0.0	0.0	11.838642218141253	3.107336732759719E-4
22	3.107336732759719E-4	0.0	0.0	12.338612698442292	3.107336732759719E-4
23	3.107336732759719E-4	0.0	0.0	12.826464565485567	3.107336732759719E-4
24	3.107336732759719E-4	0.0	0.0	13.218610461159845	3.107336732759719E-4
25	3.107336732759719E-4	0.0	0.0	13.547055953812547	3.107336732759719E-4
26	3.107336732759719E-4	0.0	0.0	13.864936501573865	3.107336732759719E-4
27	3.107336732759719E-4	0.0	0.0	14.169455501384318	3.107336732759719E-4
28	3.107336732759719E-4	0.0	0.0	14.516545014433579	3.107336732759719E-4
29	3.107336732759719E-4	0.0	0.0	14.823860617303515	3.107336732759719E-4
30	3.107336732759719E-4	0.0	0.0	15.170328663006224	3.107336732759719E-4
31	3.107336732759719E-4	0.0	0.0	15.53699439747187	3.107336732759719E-4
32	3.107336732759719E-4	0.0	0.0	15.916400212541832	3.107336732759719E-4
33	3.107336732759719E-4	0.0	0.0	16.264111192937644	3.107336732759719E-4
34	3.107336732759719E-4	0.0	0.0	16.59007081620414	3.107336732759719E-4
35	3.107336732759719E-4	0.0	0.0	16.958290219036165	3.107336732759719E-4
36	3.107336732759719E-4	0.0	0.0	17.31811981268974	3.107336732759719E-4
37	3.107336732759719E-4	0.0	0.0	17.65153704411486	3.107336732759719E-4
38	3.107336732759719E-4	0.0	0.0	17.988993813292566	3.107336732759719E-4
39	3.107336732759719E-4	0.0	0.0	18.308428029420263	3.107336732759719E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	65	0.0	45.000004	10
ACAACGA	20	7.025709E-4	45.0	13
TTGATCG	20	7.025709E-4	45.0	1
TCGTTTG	25	3.8845254E-5	45.0	1
ATAGCGG	50	2.1827873E-11	45.0	2
CAAGGTA	20	7.025709E-4	45.0	4
CGTAAGG	20	7.025709E-4	45.0	2
GTATAAG	25	3.8845254E-5	45.0	1
TCATACG	20	7.025709E-4	45.0	31
AATAGCG	25	3.8845254E-5	45.0	1
CACAACG	20	7.025709E-4	45.0	12
TATCGTG	20	7.025709E-4	45.0	1
TAAGACG	20	7.025709E-4	45.0	1
TACACGC	95	0.0	45.0	35
CTACCGG	30	2.1606138E-6	44.999996	2
ACACGCG	100	0.0	42.75	36
TTACACG	100	0.0	42.75	34
TACGAAT	70	0.0	41.785713	12
CGAATAT	70	0.0	41.785713	14
GACCTAT	70	0.0	41.785713	33
>>END_MODULE