##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390585
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19665885786704	33.0	31.0	34.0	30.0	34.0
2	32.30851159158698	34.0	31.0	34.0	30.0	34.0
3	32.28680568890255	34.0	31.0	34.0	30.0	34.0
4	35.89155241496729	37.0	35.0	37.0	35.0	37.0
5	35.94703073594736	37.0	35.0	37.0	35.0	37.0
6	35.923622258919316	37.0	35.0	37.0	35.0	37.0
7	36.09944826350218	37.0	35.0	37.0	35.0	37.0
8	35.85030403113279	37.0	35.0	37.0	35.0	37.0
9	37.78832264423877	39.0	38.0	39.0	35.0	39.0
10	37.57908009780201	39.0	37.0	39.0	35.0	39.0
11	37.5108542314733	39.0	37.0	39.0	35.0	39.0
12	37.31437715222039	39.0	37.0	39.0	35.0	39.0
13	37.191039082402035	39.0	37.0	39.0	34.0	39.0
14	38.44847344368063	40.0	38.0	41.0	34.0	41.0
15	38.39248563052857	40.0	38.0	41.0	34.0	41.0
16	38.535069191085164	40.0	38.0	41.0	34.0	41.0
17	38.38012212450555	40.0	37.0	41.0	34.0	41.0
18	38.192908073786754	40.0	37.0	41.0	34.0	41.0
19	38.00772687123161	40.0	37.0	41.0	34.0	41.0
20	37.66492056786615	40.0	35.0	41.0	33.0	41.0
21	37.72976688812935	39.0	35.0	41.0	34.0	41.0
22	37.714576852669715	39.0	35.0	41.0	34.0	41.0
23	37.61644200366118	39.0	35.0	41.0	34.0	41.0
24	37.57753881997517	39.0	35.0	41.0	33.0	41.0
25	37.499824622041295	39.0	35.0	41.0	33.0	41.0
26	37.44154793450849	39.0	35.0	41.0	33.0	41.0
27	37.365710408745855	39.0	35.0	41.0	33.0	41.0
28	37.278405468719995	39.0	35.0	41.0	33.0	41.0
29	37.17377267432185	39.0	35.0	41.0	33.0	41.0
30	36.98485604925944	39.0	35.0	41.0	33.0	41.0
31	36.81171319943162	39.0	35.0	41.0	32.0	41.0
32	36.426450068487014	39.0	35.0	41.0	31.0	41.0
33	36.019186604708324	39.0	35.0	41.0	29.0	41.0
34	35.719968764801514	39.0	35.0	41.0	27.0	41.0
35	35.518089532368116	39.0	35.0	41.0	25.0	41.0
36	35.47906089583574	39.0	35.0	41.0	25.0	41.0
37	35.46401679531984	39.0	35.0	41.0	25.0	41.0
38	35.20885850711113	38.0	35.0	41.0	23.0	41.0
39	35.182905129485256	38.0	35.0	41.0	23.0	41.0
40	35.03270222870822	38.0	35.0	41.0	23.0	41.0
41	35.02095574586838	38.0	35.0	41.0	23.0	41.0
42	34.857032400117774	38.0	35.0	40.0	23.0	41.0
43	34.74570708040503	38.0	35.0	40.0	22.0	41.0
44	34.83493733758337	38.0	35.0	40.0	22.0	41.0
45	34.80749900789841	38.0	35.0	40.0	23.0	41.0
46	34.76590754893301	38.0	35.0	40.0	23.0	41.0
47	34.60901468310355	37.0	35.0	40.0	22.0	41.0
48	34.64160426027625	37.0	35.0	40.0	23.0	41.0
49	34.67369202606347	37.0	35.0	40.0	23.0	41.0
50	34.51860414506446	37.0	34.0	40.0	22.0	41.0
51	33.752248550251544	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	3.0
11	10.0
12	5.0
13	8.0
14	12.0
15	16.0
16	34.0
17	69.0
18	123.0
19	264.0
20	525.0
21	1119.0
22	1846.0
23	2402.0
24	3344.0
25	4807.0
26	6701.0
27	7594.0
28	7232.0
29	6666.0
30	6888.0
31	7747.0
32	9578.0
33	12472.0
34	23574.0
35	35530.0
36	28192.0
37	36879.0
38	67205.0
39	119603.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.77957422840099	15.928415069703137	19.592406262401273	17.699604439494603
2	36.036970185747016	29.062303979927545	20.06682284265909	14.833902991666347
3	26.95776847549189	29.106084463049015	29.33369177003725	14.602455291421842
4	24.96742066387598	20.888923025717833	38.0145166865087	16.129139623897487
5	28.759424965116427	23.791236222589195	28.350295070215193	19.09904374207919
6	21.813689721827515	35.57458683769218	29.66575777359602	12.945965666884288
7	65.46692781340809	5.619007386356363	24.08515431980235	4.828910480433196
8	64.59592662288618	12.331246719664094	17.449466825402922	5.623359832046802
9	59.87198689145769	6.595491378317139	18.699130790993	14.833390939232178
10	38.337877798686584	20.99927032528131	25.343779202990387	15.31907267304172
11	32.590601277570826	20.498995097097943	29.4911478935443	17.419255731786933
12	26.538653558124352	18.929042333934994	33.716348554091944	20.815955553848713
13	25.190419498956697	21.059948538730367	37.495039492043986	16.254592470268957
14	19.84331195514421	28.1052779804652	32.1545886298757	19.896821434514894
15	16.516763316563615	21.464725987941165	41.70641473686906	20.312095958626163
16	19.311289476042344	21.171319943162178	35.414058399580114	24.103332181215357
17	20.137998131008615	21.92506112625933	35.57893928338262	22.35800145934944
18	20.547384052126937	21.56252800286749	34.86001766580898	23.03007027919659
19	21.21663658358616	23.775874649564116	32.07342831905987	22.934060447789854
20	24.290231319687138	23.493989784553936	35.045124620761165	17.17065427499776
21	21.824954875379238	27.2857380595773	32.7142619404227	18.175045124620762
22	19.319994367423224	21.64266420881498	35.41149813740927	23.62584328635252
23	20.407337711381647	26.00304671198331	33.97928747903785	19.610328097597193
24	22.623244620249114	22.415095305759312	32.425464367551236	22.53619570644034
25	19.26622886183545	28.079931384973822	31.33888910224407	21.314950650946656
26	17.907497727767325	23.950996582050003	35.79886580385831	22.34263988632436
27	21.6923332949294	23.65554232753434	32.6226045547064	22.029519822829858
28	16.624038301522077	26.026601123955096	35.70157584136616	21.647784733156676
29	20.969827310316578	24.355261978826633	32.81872063699323	21.856190073863562
30	19.65257242341616	25.684294071712944	32.97310444589526	21.69002905897564
31	21.983947156188794	24.67043025205781	29.06076782262504	24.28485476912836
32	25.426731697325806	27.445242392821022	27.899688928146244	19.228336981706924
33	23.05772111064173	23.087164125606463	29.080225815123466	24.77488894862834
34	21.79960827988786	26.44546001510555	28.614258100029446	23.14067360497715
35	21.303941523612018	25.690950753357143	28.05842518273871	24.946682540292127
36	21.573025077767962	30.287901481111668	27.312620812371186	20.826452628749184
37	21.985227287274217	26.25881690285085	29.095075335714377	22.660880474160557
38	21.960136717999923	27.664912887079634	27.572231396495	22.80271899842544
39	24.982014158249804	24.064160170001408	28.059961340041223	22.893864331707565
40	20.63033654646236	24.77540100106251	31.710383143233866	22.883879309241266
41	21.24940793937299	25.764686303877514	26.646184569299898	26.339721187449594
42	21.482903849354173	26.89145768526697	29.679327163101505	21.94631130227735
43	24.95077895976548	24.477130458158918	26.82002637070036	23.752064211375245
44	22.064851440787535	23.246668458850188	30.10893915536951	24.579540944992768
45	20.71226493592944	23.530857559814127	29.162922283241805	26.59395522101463
46	25.403433311571106	26.032745753165127	27.336431250560057	21.227389684703713
47	18.39215535670853	25.648962453755264	34.51847869221808	21.440403497318126
48	21.764020635713095	24.548049720291356	28.66341513370969	25.024514510285854
49	20.974691808441186	22.152668433247563	33.951892673809795	22.920747084501453
50	21.994444231089265	22.376179320762446	31.185785424427458	24.443591023720828
51	20.96291460245529	22.4852464892405	28.435039748070203	28.116799160234006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434.0
1	762.5
2	1091.0
3	6830.5
4	12570.0
5	8004.0
6	3438.0
7	3397.0
8	3356.0
9	3316.0
10	3276.0
11	3140.5
12	3005.0
13	2981.5
14	2958.0
15	2855.0
16	2752.0
17	2633.5
18	2515.0
19	2495.0
20	2475.0
21	2304.5
22	2134.0
23	2074.0
24	2014.0
25	2358.0
26	2862.5
27	3023.0
28	3141.5
29	3260.0
30	3981.0
31	4702.0
32	5189.0
33	5676.0
34	6645.5
35	7615.0
36	7792.5
37	7970.0
38	8672.0
39	9374.0
40	10738.0
41	12102.0
42	14278.0
43	16454.0
44	18153.0
45	19852.0
46	35559.0
47	51266.0
48	42126.0
49	32986.0
50	33377.0
51	33768.0
52	30084.0
53	26400.0
54	23821.5
55	21243.0
56	19407.5
57	17572.0
58	16672.0
59	15772.0
60	14761.5
61	13751.0
62	12640.0
63	11529.0
64	10504.5
65	9480.0
66	7932.5
67	6385.0
68	5369.5
69	4354.0
70	3903.5
71	3453.0
72	3122.5
73	2792.0
74	2423.5
75	1724.0
76	1393.0
77	1138.5
78	884.0
79	642.0
80	400.0
81	314.5
82	229.0
83	150.5
84	72.0
85	58.0
86	44.0
87	25.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	390585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.436557527310015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98995172444562	25.742789498592465
2	8.604322984679232	5.753978809215661
3	2.8955245161290737	2.9044911616585884
4	1.5682262107451292	2.097443436456597
5	1.072669641314615	1.7933190084807562
6	0.8061791396548621	1.6173513108232254
7	0.6185689035993666	1.4477970310863713
8	0.5877960359642607	1.5723100776675034
9	0.4949274202133981	1.489380224202758
>10	6.212206483822748	41.013401255979055
>50	0.10742446933557862	2.4440344127911873
>100	0.03222734080067358	2.1066519592093678
>500	0.0023019529143338277	0.4985050245699429
>1k	0.0046039058286676555	2.2790633727507994
>5k	0.0030692705524451034	7.239483416515738
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7798	1.9964924408259408	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	7391	1.8922897704724964	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGC	7039	1.8021685420587068	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	5989	1.5333410141198458	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	2157	0.5522485502515457	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGTCAGT	1684	0.431148149570516	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCT	1460	0.37379827694355905	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTA	1405	0.3597168350039044	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	1141	0.2921259136935622	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTC	1036	0.26524316089967614	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	729	0.18664311225469488	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	633	0.16206459541457044	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	581	0.14875123212616972	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATG	462	0.1182841122930988	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	444	0.11367564038557548	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16923332949294007	0.0
2	0.0	0.0	0.0	1.0458670967907113	0.0
3	0.0	0.0	0.0	1.3303122239717347	0.0
4	0.0	0.0	0.0	2.0000768078651254	0.0
5	0.0	0.0	0.0	4.503501158518632	0.0
6	0.0	0.0	0.0	5.143822727447291	0.0
7	0.0	0.0	0.0	6.101872831777974	0.0
8	0.0	0.0	0.0	7.197409014683103	0.0
9	0.0	0.0	0.0	7.565830741067885	0.0
10	0.0	0.0	0.0	10.259482571015273	0.0
11	0.0	0.0	0.0	11.479191469206446	0.0
12	0.0	0.0	0.0	14.283958677368563	0.0
13	0.0	0.0	0.0	14.80369189805036	0.0
14	0.0	0.0	0.0	15.046916804280759	0.0
15	0.0	0.0	0.0	15.746124403138882	0.0
16	0.0	0.0	0.0	16.4670942304492	0.0
17	0.0	0.0	0.0	17.20265755213334	0.0
18	2.560262170846295E-4	0.0	0.0	17.964079521743027	0.0
19	2.560262170846295E-4	0.0	0.0	18.87527682834722	0.0
20	2.560262170846295E-4	0.0	0.0	19.41651625126413	0.0
21	2.560262170846295E-4	0.0	0.0	19.956475543095614	0.0
22	2.560262170846295E-4	0.0	0.0	20.486449812460798	0.0
23	2.560262170846295E-4	0.0	0.0	21.00157456123507	0.0
24	2.560262170846295E-4	0.0	0.0	21.392270568506216	0.0
25	2.560262170846295E-4	0.0	0.0	21.768885133837706	0.0
26	2.560262170846295E-4	0.0	0.0	22.13321044074913	0.0
27	2.560262170846295E-4	0.0	0.0	22.51468950420523	0.0
28	2.560262170846295E-4	0.0	0.0	22.88464738789252	0.0
29	2.560262170846295E-4	0.0	0.0	23.24794858993561	0.0
30	2.560262170846295E-4	0.0	0.0	23.64555730506804	0.0
31	5.12052434169259E-4	0.0	0.0	24.020891739314106	0.0
32	5.12052434169259E-4	0.0	0.0	24.399298488165186	0.0
33	5.12052434169259E-4	0.0	0.0	24.772840738891663	0.0
34	5.12052434169259E-4	0.0	0.0	25.13716604580309	0.0
35	5.12052434169259E-4	0.0	0.0	25.52094934521295	0.0
36	5.12052434169259E-4	0.0	0.0	25.883994521038954	0.0
37	5.12052434169259E-4	0.0	0.0	26.22450938976151	0.0
38	5.12052434169259E-4	0.0	0.0	26.565792337135324	0.0
39	5.12052434169259E-4	0.0	0.0	26.958024501708977	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTTA	20	7.02756E-4	45.000004	42
TAGGTCG	20	7.02756E-4	45.000004	2
AACTTCG	20	7.02756E-4	45.000004	11
TTAGACG	20	7.02756E-4	45.000004	1
CGTCTCA	20	7.02756E-4	45.000004	31
CCCGTCG	35	1.2091732E-7	45.000004	40
ATATCAG	20	7.02756E-4	45.000004	1
CGTGTCG	20	7.02756E-4	45.000004	29
CGTCGAA	20	7.02756E-4	45.000004	42
CAAGACG	20	7.02756E-4	45.000004	38
CGATGCG	20	7.02756E-4	45.000004	10
CCTGCGT	20	7.02756E-4	45.000004	45
GCGTCGC	20	7.02756E-4	45.000004	26
TGGCTAG	20	7.02756E-4	45.000004	1
AGTCGAT	20	7.02756E-4	45.000004	20
AAGTCGA	20	7.02756E-4	45.000004	19
CATGCGG	25	3.8860606E-5	45.0	2
ATAGCGG	25	3.8860606E-5	45.0	2
CCGTCGA	25	3.8860606E-5	45.0	41
ACCTATG	25	3.8860606E-5	45.0	34
>>END_MODULE