##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446351
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.360312847960465	33.0	31.0	34.0	31.0	34.0
2	32.43841729938994	34.0	31.0	34.0	31.0	34.0
3	32.44368893538941	34.0	31.0	34.0	30.0	34.0
4	35.97148208472704	37.0	35.0	37.0	35.0	37.0
5	36.03928970697948	37.0	35.0	37.0	35.0	37.0
6	36.02110894789079	37.0	35.0	37.0	35.0	37.0
7	36.190099271649444	37.0	36.0	37.0	35.0	37.0
8	36.01834206711758	37.0	36.0	37.0	35.0	37.0
9	37.92238395343575	39.0	38.0	39.0	35.0	39.0
10	37.617630519479064	39.0	37.0	39.0	35.0	39.0
11	37.57834977405674	39.0	37.0	39.0	35.0	39.0
12	37.50046712116698	39.0	37.0	39.0	35.0	39.0
13	37.48020728081711	39.0	37.0	39.0	35.0	39.0
14	38.75206283843881	40.0	38.0	41.0	35.0	41.0
15	38.799420187251734	40.0	38.0	41.0	35.0	41.0
16	38.81039809477295	40.0	38.0	41.0	35.0	41.0
17	38.75806484134683	40.0	38.0	41.0	35.0	41.0
18	38.681889365096076	40.0	38.0	41.0	35.0	41.0
19	38.59361130590052	40.0	38.0	41.0	35.0	41.0
20	38.49871065596358	40.0	37.0	41.0	35.0	41.0
21	38.41391640211403	40.0	37.0	41.0	34.0	41.0
22	38.41226075442869	40.0	37.0	41.0	34.0	41.0
23	38.3745706854023	40.0	37.0	41.0	34.0	41.0
24	38.301632571675654	40.0	37.0	41.0	34.0	41.0
25	38.14741761528483	40.0	37.0	41.0	34.0	41.0
26	38.14206084449234	40.0	37.0	41.0	34.0	41.0
27	38.140382792914096	40.0	37.0	41.0	34.0	41.0
28	38.054548998433965	40.0	36.0	41.0	34.0	41.0
29	38.000374145011435	40.0	36.0	41.0	34.0	41.0
30	37.83815875846587	40.0	36.0	41.0	33.0	41.0
31	37.81606403928747	40.0	36.0	41.0	33.0	41.0
32	37.689096697442146	40.0	36.0	41.0	33.0	41.0
33	37.58703352294495	40.0	36.0	41.0	33.0	41.0
34	37.45438903463866	40.0	36.0	41.0	33.0	41.0
35	37.3421007234217	40.0	35.0	41.0	33.0	41.0
36	37.25267558490963	40.0	35.0	41.0	33.0	41.0
37	37.19796527844678	40.0	35.0	41.0	33.0	41.0
38	37.0949521788906	40.0	35.0	41.0	32.0	41.0
39	37.06106181010012	40.0	35.0	41.0	32.0	41.0
40	36.97291145309409	39.0	35.0	41.0	32.0	41.0
41	36.86919263091155	39.0	35.0	41.0	32.0	41.0
42	36.75308445595507	39.0	35.0	41.0	31.0	41.0
43	36.6342049194468	39.0	35.0	41.0	31.0	41.0
44	36.64474595105646	39.0	35.0	41.0	31.0	41.0
45	36.60157364943733	39.0	35.0	41.0	31.0	41.0
46	36.5179668019115	39.0	35.0	41.0	31.0	41.0
47	36.446325873583795	39.0	35.0	41.0	31.0	41.0
48	36.4052147301115	39.0	35.0	41.0	31.0	41.0
49	36.34061086454383	39.0	35.0	41.0	31.0	41.0
50	36.24838747980849	38.0	35.0	41.0	31.0	41.0
51	35.436107458031906	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	10.0
11	10.0
12	9.0
13	9.0
14	20.0
15	30.0
16	40.0
17	63.0
18	105.0
19	204.0
20	381.0
21	574.0
22	927.0
23	1348.0
24	1884.0
25	2623.0
26	3439.0
27	4030.0
28	4446.0
29	4622.0
30	5276.0
31	6561.0
32	8341.0
33	11774.0
34	23273.0
35	34094.0
36	31129.0
37	46568.0
38	82953.0
39	171388.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31198765097423	19.92445407313975	22.979000831184425	12.78455744470159
2	33.71606650371569	25.29265085101187	23.903833530114195	17.08744911515825
3	32.87726475352357	24.218832264294242	26.408812795311317	16.49509018687087
4	28.67362232861582	24.543688711350484	27.54446612643413	19.238222833599565
5	26.342721311255048	29.58770115895338	23.984039466697734	20.085538063093843
6	26.330847248017815	35.276273605301654	23.997929880296002	14.394949266384527
7	77.77914690456613	6.765527578071966	10.851997643110467	4.60332787425143
8	77.99310408176525	7.726654583500429	9.63725857004913	4.6429827646851916
9	71.45139139376859	9.017118814565219	11.614402118512112	7.917087673154087
10	39.613667270824976	25.4763627727954	18.489484732867183	16.420485223512436
11	31.4104818853324	24.652123552988567	23.93228647409774	20.0051080875813
12	28.221511769885133	22.31450136775766	29.05874524757422	20.405241614782984
13	25.85230009566462	23.191837813738513	30.202239941212184	20.753622149384675
14	21.400422537420102	27.22185006866793	28.748451330903258	22.629276063008707
15	20.810303998422768	24.981012700766886	32.50961687102751	21.69906642978284
16	23.050245210607795	23.48107207108307	30.919612591883965	22.549070126425168
17	22.953460393277936	23.845359369644072	29.045078872904956	24.15610136417304
18	24.10098778763798	24.362665256715008	28.523068168324926	23.013278787322086
19	25.567546616900156	25.840650071356396	26.223084523166744	22.368718788576704
20	27.282564618428097	24.653915864420604	27.13425084742725	20.929268669724053
21	26.30508277118232	26.11039294187758	26.725155763065388	20.859368523874707
22	24.23944384576264	24.21345532999814	26.484089875456757	25.06301094878246
23	24.703876545588564	26.241231676416092	27.266433815539788	21.788457962455556
24	24.18119372422152	25.22857571731664	27.59442680760209	22.99580375085975
25	24.14691576808386	26.267892308967607	26.26430768610354	23.320884236844993
26	22.403220783643366	26.737253865231626	27.151277806031576	23.708247545093435
27	24.662877421580774	25.952445496929542	27.166736492132877	22.217940589356807
28	22.178285698923045	26.50089279513208	28.281330163929287	23.039491342015587
29	23.355610270840664	25.880080922861154	27.978429531915467	22.78587927438272
30	24.19822068282585	25.722805594700137	27.787772403332806	22.291201319141212
31	24.89498175202923	25.6271409720153	25.30071625245603	24.177161023499444
32	25.11924471996254	26.55511021595112	25.895763647891457	22.429881416194878
33	25.183095814728766	25.63610252917547	25.559705254385	23.621096401710762
34	22.901483361748937	25.899796348613535	27.800990700144055	23.397729589493473
35	24.60373114432364	24.601714793962596	26.441970556803952	24.352583504909813
36	24.13212919876958	26.741062527024695	25.967456105172836	23.15935216903289
37	23.84491129178606	27.884109142804654	26.13078048441697	22.14019908099231
38	22.722700296403502	27.26598573768178	25.874703988565052	24.136609977349664
39	23.992328907070892	25.252099804862095	27.383158097551032	23.372413190515985
40	23.460908567472686	24.364681607076047	29.385394006062494	22.789015819388776
41	21.751939617027855	24.927243357805853	28.734112839446986	24.58670418571931
42	22.526442194595734	25.83706544849233	27.431550506215963	24.204941850695977
43	24.765039173206738	24.472220292998113	26.484986031172774	24.277754502622376
44	23.38831995447529	25.045087834462116	27.56776617505058	23.998826036012016
45	23.16920988190908	24.348326765258733	27.094147879135477	25.38831547369671
46	23.246503312415566	25.702866130018755	27.43110242835795	23.61952812920773
47	22.372527450369777	25.476586811724406	29.558352059253817	22.592533678652003
48	22.768628276849384	24.501121314839665	28.228681015613272	24.501569392697675
49	22.30710808310052	25.09460043777207	28.743970552323173	23.85432092680424
50	21.401542732065124	24.503137665200704	29.086077996912746	25.009241605821426
51	21.839314799339533	24.53360695954529	27.249630895864463	26.377447345250708
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	334.0
1	691.5
2	1049.0
3	2697.0
4	4345.0
5	2887.0
6	1429.0
7	1469.5
8	1510.0
9	1605.0
10	1700.0
11	1569.0
12	1438.0
13	1440.5
14	1443.0
15	1407.5
16	1372.0
17	1407.5
18	1443.0
19	1404.0
20	1365.0
21	1408.5
22	1452.0
23	1800.0
24	2148.0
25	2441.5
26	3344.0
27	3953.0
28	4709.0
29	5465.0
30	6386.5
31	7308.0
32	8083.5
33	8859.0
34	9928.5
35	10998.0
36	11018.5
37	11039.0
38	11979.0
39	12919.0
40	14753.0
41	16587.0
42	17931.5
43	19276.0
44	21534.0
45	23792.0
46	29602.0
47	35412.0
48	35570.0
49	35728.0
50	35246.5
51	34765.0
52	31303.5
53	27842.0
54	26499.0
55	25156.0
56	24927.0
57	24698.0
58	24267.0
59	23836.0
60	23192.0
61	22548.0
62	21382.5
63	20217.0
64	17962.0
65	15707.0
66	13595.5
67	11484.0
68	9765.5
69	8047.0
70	6749.5
71	5452.0
72	4967.0
73	4482.0
74	3777.5
75	2456.0
76	1839.0
77	1487.5
78	1136.0
79	791.0
80	446.0
81	366.5
82	287.0
83	202.5
84	118.0
85	92.5
86	67.0
87	53.0
88	39.0
89	24.0
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.414766814528033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13550802840031	21.63569888916782
2	7.796576872322332	4.742621350464521
3	3.1408979449364702	2.8658903575041914
4	2.0441252410357236	2.4868637018316977
5	1.5547588696243204	2.364381423622145
6	1.3285397210078862	2.4244335490975795
7	1.2410642624920594	2.6422676101878135
8	1.1080560105212036	2.6961012141950906
9	0.9998439771100536	2.736901927324132
>10	9.512985493224773	48.76512371982196
>50	0.08806228999818329	1.823783756905535
>100	0.04292111613356833	2.512738721719535
>500	0.0029600769747288504	0.5984747636198714
>1k	0.003700096218411063	1.7047190145381368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2609	0.5845175657722286	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG	1429	0.3201516295471501	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	1298	0.2908025298475863	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	1233	0.2762399994623066	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	1005	0.22515912364932533	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	964	0.21597352756014884	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCC	664	0.1487618488588577	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	522	0.11694832094024658	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	509	0.11403581486319062	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09633673947185063	0.0
2	0.0	0.0	0.0	0.520666471006002	0.0
3	0.0	0.0	0.0	0.7155803392397463	0.0
4	0.0	0.0	0.0	1.0328194627098404	0.0
5	0.0	0.0	0.0	2.029568657849988	0.0
6	0.0	0.0	0.0	2.533208170251663	0.0
7	0.0	0.0	0.0	3.0122034004628646	0.0
8	0.0	0.0	0.0	3.7087404307372447	0.0
9	0.0	0.0	0.0	4.0158978024021454	0.0
10	0.0	0.0	0.0	5.006821985388181	0.0
11	2.240389290043038E-4	0.0	0.0	5.8931199885292065	0.0
12	2.240389290043038E-4	0.0	0.0	6.820193076749016	0.0
13	2.240389290043038E-4	0.0	0.0	7.104722516584482	0.0
14	2.240389290043038E-4	0.0	0.0	7.231752589329922	0.0
15	2.240389290043038E-4	0.0	0.0	7.497462759129026	0.0
16	2.240389290043038E-4	0.0	0.0	7.941507916415556	0.0
17	2.240389290043038E-4	0.0	0.0	8.45545321955143	0.0
18	2.240389290043038E-4	0.0	0.0	9.021823632074309	0.0
19	2.240389290043038E-4	0.0	0.0	9.39148786493141	0.0
20	2.240389290043038E-4	0.0	0.0	9.742108788823145	0.0
21	2.240389290043038E-4	0.0	0.0	10.12812786349756	0.0
22	2.240389290043038E-4	0.0	0.0	10.549097011096649	0.0
23	2.240389290043038E-4	0.0	0.0	10.966033457973657	0.0
24	2.240389290043038E-4	0.0	0.0	11.282600464656738	0.0
25	2.240389290043038E-4	0.0	0.0	11.566457787705192	0.0
26	2.240389290043038E-4	0.0	0.0	11.846506448960572	0.0
27	2.240389290043038E-4	0.0	0.0	12.11602528055275	0.0
28	2.240389290043038E-4	0.0	0.0	12.417581678992542	0.0
29	2.240389290043038E-4	0.0	0.0	12.715105376710257	0.0
30	2.240389290043038E-4	0.0	0.0	13.048251264139656	0.0
31	2.240389290043038E-4	0.0	0.0	13.352496129727502	0.0
32	2.240389290043038E-4	0.0	0.0	13.67152756462963	0.0
33	2.240389290043038E-4	0.0	0.0	13.974652235572453	0.0
34	2.240389290043038E-4	0.0	0.0	14.286962502604453	0.0
35	2.240389290043038E-4	0.0	0.0	14.615403572524762	0.0
36	2.240389290043038E-4	0.0	0.0	14.923681138834684	0.0
37	2.240389290043038E-4	0.0	0.0	15.241816418020795	0.0
38	2.240389290043038E-4	0.0	0.0	15.552110334691756	0.0
39	2.240389290043038E-4	0.0	0.0	15.871813886380897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	20	7.028645E-4	45.0	2
GGCGTAT	20	7.028645E-4	45.0	19
TGAGTAG	20	7.028645E-4	45.0	1
CGGTCTA	25	3.8869584E-5	45.0	31
TAGTGCG	55	1.8189894E-12	45.0	1
CTTACGG	40	6.7993824E-9	45.0	2
AAATGCG	20	7.028645E-4	45.0	1
AGCGTTG	30	2.1625074E-6	44.999996	1
AGGGTAC	75	0.0	42.0	6
CCGATGA	165	0.0	40.909092	18
TTGCGAG	55	6.002665E-11	40.909092	1
TAGGGTC	75	0.0	39.0	5
CGTTTTT	1235	0.0	38.805668	1
CAAACGC	35	6.239705E-6	38.571426	34
TTGTGCG	35	6.239705E-6	38.571426	1
CCGTCGA	35	6.239705E-6	38.571426	41
CGTAAGG	100	0.0	38.25	2
TTACGGG	100	0.0	38.25	3
AACGGGC	65	9.094947E-12	38.07692	4
CTACGAA	125	0.0	37.800003	11
>>END_MODULE