FastQCFastQC Report
Sat 18 Jun 2016
SRR3551976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733355
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145361.982123255449271No Hit
GAATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTC136171.8568087761043424No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG126491.7248126759891185No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC102981.4042312386224953No Hit
GAACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCT34190.46621349823755204No Hit
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC29490.40212448268573886No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCT25360.3458079647646774No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAACCGATT19720.26890114610250154No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTC18850.2570378602450382No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTA17250.2352203230359103No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTT13110.17876744550729184No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCC8950.12204184876355925No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG253.889419E-545.0000041
TATAGCG302.1644228E-645.0000041
TAGTGCG253.889419E-545.0000041
CGTTTTT55100.043.0399251
TACGGCT10950.042.5342457
CGATGAA19500.041.88461319
TACGGGT650.041.538464
GATGAAT21500.041.2325620
CCGATGA18850.040.94164718
CGACGGT556.002665E-1140.9090928
ACGGCTG11650.040.557948
ATGAATG21950.040.2847421
CGTATGG451.9266736E-840.02
TGAATGA22000.039.78409222
TGATACC14300.039.650354
ACCGATG19400.039.4329917
CGCATCG403.4559343E-739.37521
TCTCGAC403.4559343E-739.37517
CTCGACC403.4559343E-739.37518
GACACGA750.039.00000425
Produced by FastQC (version 0.11.5)