##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733355
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.135530541143105	33.0	31.0	34.0	30.0	34.0
2	32.23934247397236	34.0	31.0	34.0	30.0	34.0
3	32.230394556524466	34.0	31.0	34.0	30.0	34.0
4	35.86609077459075	37.0	35.0	37.0	35.0	37.0
5	35.87565912825303	37.0	35.0	37.0	35.0	37.0
6	35.841749221045745	37.0	35.0	37.0	35.0	37.0
7	36.00981925534018	37.0	35.0	37.0	35.0	37.0
8	35.74106537761384	37.0	35.0	37.0	35.0	37.0
9	37.628522339112706	39.0	37.0	39.0	35.0	39.0
10	37.40395988300346	39.0	37.0	39.0	34.0	39.0
11	37.363780161040694	39.0	37.0	39.0	34.0	39.0
12	37.284857947378825	39.0	37.0	39.0	34.0	39.0
13	37.231317711067625	39.0	37.0	39.0	34.0	39.0
14	38.561554772245366	40.0	38.0	41.0	34.0	41.0
15	38.50053793865181	40.0	38.0	41.0	34.0	41.0
16	38.5959978455182	40.0	38.0	41.0	34.0	41.0
17	38.44122014576842	40.0	38.0	41.0	34.0	41.0
18	38.29220909382223	40.0	38.0	41.0	34.0	41.0
19	38.150130564324236	40.0	37.0	41.0	34.0	41.0
20	37.77659796415106	40.0	35.0	41.0	33.0	41.0
21	37.85744966625986	40.0	35.0	41.0	33.0	41.0
22	37.811643746889295	40.0	35.0	41.0	33.0	41.0
23	37.68071261530909	40.0	35.0	41.0	33.0	41.0
24	37.67045700922473	40.0	35.0	41.0	33.0	41.0
25	37.65349251044856	40.0	35.0	41.0	33.0	41.0
26	37.57638115237504	40.0	35.0	41.0	33.0	41.0
27	37.48211711926693	40.0	35.0	41.0	33.0	41.0
28	37.367505505519155	40.0	35.0	41.0	33.0	41.0
29	37.292098642540104	40.0	35.0	41.0	33.0	41.0
30	37.17619706690484	40.0	35.0	41.0	32.0	41.0
31	37.06267360282537	40.0	35.0	41.0	32.0	41.0
32	36.61946806117092	40.0	35.0	41.0	30.0	41.0
33	36.364318781490546	39.0	35.0	41.0	30.0	41.0
34	36.16268246619986	39.0	35.0	41.0	28.0	41.0
35	35.85218618540816	39.0	35.0	41.0	26.0	41.0
36	35.80853065704877	39.0	35.0	41.0	25.0	41.0
37	35.6969216818594	39.0	35.0	41.0	25.0	41.0
38	35.67341055832441	39.0	35.0	41.0	24.0	41.0
39	35.51459252340272	39.0	35.0	41.0	24.0	41.0
40	35.453842954639974	39.0	35.0	41.0	24.0	41.0
41	35.36944453913862	39.0	35.0	41.0	23.0	41.0
42	35.13853863408581	39.0	35.0	41.0	23.0	41.0
43	35.058742355339504	39.0	35.0	41.0	22.0	41.0
44	35.229440039271566	39.0	35.0	41.0	23.0	41.0
45	35.22100074315986	39.0	35.0	41.0	23.0	41.0
46	35.12688125123576	38.0	35.0	41.0	23.0	41.0
47	34.98908441341506	38.0	35.0	41.0	23.0	41.0
48	34.998251869831115	38.0	35.0	40.0	23.0	41.0
49	34.96206884796585	38.0	35.0	40.0	23.0	41.0
50	34.83696163522441	38.0	35.0	40.0	23.0	41.0
51	34.163612438723405	37.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	12.0
10	5.0
11	12.0
12	13.0
13	9.0
14	24.0
15	40.0
16	66.0
17	121.0
18	236.0
19	604.0
20	1449.0
21	2931.0
22	4490.0
23	6017.0
24	7384.0
25	8916.0
26	11143.0
27	12615.0
28	12220.0
29	11354.0
30	11865.0
31	13476.0
32	16808.0
33	21804.0
34	35808.0
35	51582.0
36	52927.0
37	73159.0
38	129183.0
39	246785.0
40	292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.729012551901874	16.793776547511097	20.296173067613914	17.181037832973118
2	33.3138793626552	31.41507182742328	20.62711783515487	14.643930974766656
3	27.75531632019963	30.38542043076	27.88458522816371	13.974678020876654
4	24.425687422871597	21.487410599232295	37.46725664923537	16.619645328660745
5	29.77862017713113	25.293207246149546	26.492217275398684	18.435955301320643
6	22.478199507741817	37.59529832073144	26.69321133693777	13.23329083458898
7	68.72537856836048	5.3973859863231315	21.89430766818253	3.9829277771338574
8	68.2703465579426	12.421405731194305	14.752745941597182	4.555501769265908
9	62.97713931179306	7.099699327065337	15.41586271314711	14.50729864799449
10	37.97901425639697	24.693361332506086	22.047030428646426	15.280593982450519
11	30.58968712288046	20.921381868262984	29.789392586128137	18.69953842272842
12	23.848886282905276	19.22643194632886	33.538599995909216	23.386081774856653
13	24.952444586864477	20.15122280478077	37.10890360057544	17.787429007779316
14	19.91859331428844	28.04235329410722	31.4280259901412	20.61102740146314
15	18.034785335887804	22.536970498598905	37.449393540645396	21.978850624867903
16	19.868003899884776	22.79973546236134	31.65356478104056	25.678695856713325
17	19.951865058532363	25.387295375363912	33.02070620640754	21.64013335969619
18	20.02781735994164	23.19026937840473	32.311772606718435	24.470140654935197
19	20.955062691329573	23.98510953085477	30.119655555631308	24.940172222184344
20	24.863810841952397	23.368900464304467	33.24419960319354	18.523089090549597
21	22.344567092335907	28.283709799483198	30.870860633663096	18.5008624745178
22	20.7330692502267	21.075195505587335	34.033449011733744	24.158286232452223
23	23.511396254201582	24.934036039844276	31.5778851988464	19.976682507107746
24	21.873308288618745	25.177301579726052	30.400829066413948	22.548561065241255
25	20.55866531216123	30.326649439902916	28.734378302459245	20.38030694547661
26	19.772279455379728	23.533350151018265	33.36610509234955	23.32826530125246
27	24.96021708449523	24.352871392436132	28.99346155681764	21.693449966250995
28	19.21429594125628	26.51267121653224	32.25381977350669	22.019213068704786
29	21.744448459477333	22.76864547183833	31.1118080602164	24.375098008467933
30	20.023726571714924	29.465538518180146	29.676486831070903	20.83424807903403
31	22.452563901521092	25.206209816528148	26.38831125444021	25.952915027510553
32	24.26737391849786	26.06350266923932	26.58889623715663	23.08022717510619
33	22.99650237606616	24.074015994981966	26.21527091245031	26.714210716501558
34	22.720646889978248	24.445323206359813	26.560942517607433	26.273087386054506
35	23.11949874208262	26.99333883317084	26.61834991238895	23.268812512357588
36	20.463349946478854	29.90107110471736	27.5303229677305	22.105255981073284
37	20.774931649746712	27.40187221741176	28.684743405308478	23.13845272753305
38	20.269173865317615	27.11142625331524	26.22195253322061	26.397447348146528
39	23.794478799490015	24.601454957012635	27.61309324951763	23.990972993979724
40	20.269992022962956	26.00554983602757	29.322633649460357	24.401824491549114
41	20.910745818873533	26.06486626531489	29.016506330494778	24.0078815853168
42	20.64239011120126	26.427446461809083	30.645049123548624	22.285114303441034
43	22.272432859938228	24.416824048380388	28.8389661214555	24.471776970225882
44	22.182844597773247	24.048244029153683	29.554172263092227	24.21473910998084
45	21.156602191298894	23.67734589659851	29.03873294652658	26.12731896557602
46	24.5069577489756	25.88555338137737	27.616502239706552	21.99098662994048
47	19.84605000306809	25.250253969769076	33.156656735141915	21.74703929202092
48	21.55095417635388	25.47156561283417	28.76165022397065	24.215829986841296
49	20.32071779697418	23.890612322817734	33.16852002099938	22.620149859208706
50	20.76034117173811	23.261040014726838	31.34539206796163	24.633226745573424
51	21.067832086779255	23.064682179844688	29.49949206046185	26.36799367291421
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	474.0
1	985.0
2	1496.0
3	11423.5
4	21351.0
5	13564.0
6	5777.0
7	5463.5
8	5150.0
9	5030.0
10	4910.0
11	4769.0
12	4628.0
13	4378.0
14	4128.0
15	3894.0
16	3660.0
17	3487.0
18	3314.0
19	3146.5
20	2979.0
21	3016.5
22	3054.0
23	3134.5
24	3215.0
25	3786.5
26	5128.5
27	5899.0
28	7083.0
29	8267.0
30	9555.5
31	10844.0
32	12029.5
33	13215.0
34	14271.0
35	15327.0
36	16796.5
37	18266.0
38	19436.0
39	20606.0
40	23287.0
41	25968.0
42	28494.5
43	31021.0
44	33867.5
45	36714.0
46	65220.5
47	93727.0
48	74857.5
49	55988.0
50	55696.5
51	55405.0
52	50229.0
53	45053.0
54	42718.5
55	40384.0
56	38159.0
57	35934.0
58	34041.0
59	32148.0
60	30821.5
61	29495.0
62	27500.0
63	25505.0
64	23142.0
65	20779.0
66	17531.5
67	14284.0
68	12073.5
69	9863.0
70	8508.5
71	7154.0
72	6271.0
73	5388.0
74	4497.5
75	2763.5
76	1920.0
77	1560.5
78	1201.0
79	832.5
80	464.0
81	371.0
82	278.0
83	178.5
84	79.0
85	64.5
86	50.0
87	34.5
88	19.0
89	12.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	733355.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.40426238592533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.992854835684	21.888896483359694
2	7.551211928282815	4.591780575984798
3	3.153592362130644	2.8764794890941046
4	2.093323701663854	2.5458385233625718
5	1.6931337538955225	2.5739241453953094
6	1.4437694419272797	2.6338046962282804
7	1.2840218138053863	2.732781529533351
8	1.1670542902755967	2.8386739888127286
9	1.0545709606198588	2.8857106972137165
>10	8.445361168347567	40.44908077507782
>50	0.06733117822270362	1.4512816275758438
>100	0.04609248440982041	2.822600563419157
>500	0.002711322612342377	0.5179713845448901
>1k	0.00316320971439944	2.1703962763012274
>5k	0.0	0.0
>10k+	0.0018075484082282515	7.02077924409652
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14536	1.982123255449271	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTC	13617	1.8568087761043424	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG	12649	1.7248126759891185	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC	10298	1.4042312386224953	No Hit
GAACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCT	3419	0.46621349823755204	No Hit
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2949	0.40212448268573886	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCT	2536	0.3458079647646774	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAACCGATT	1972	0.26890114610250154	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTC	1885	0.2570378602450382	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTA	1725	0.2352203230359103	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTT	1311	0.17876744550729184	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCC	895	0.12204184876355925	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.727192151140989E-4	0.0	0.0	0.07090699592966572	0.0
2	2.727192151140989E-4	0.0	0.0	0.59848231756789	0.0
3	2.727192151140989E-4	0.0	0.0	0.7878858124646317	0.0
4	2.727192151140989E-4	0.0	0.0	1.4744564365143757	0.0
5	2.727192151140989E-4	0.0	0.0	3.9496560328899375	0.0
6	2.727192151140989E-4	0.0	0.0	4.704133741503092	0.0
7	2.727192151140989E-4	0.0	0.0	5.494201307688637	0.0
8	4.0907882267114833E-4	0.0	0.0	6.346312495312638	0.0
9	4.0907882267114833E-4	0.0	0.0	6.6983930020249405	0.0
10	4.0907882267114833E-4	0.0	0.0	9.001915852486176	0.0
11	4.0907882267114833E-4	0.0	0.0	9.671986964021517	0.0
12	4.0907882267114833E-4	0.0	0.0	11.64292873165111	0.0
13	4.0907882267114833E-4	0.0	0.0	11.962146572942164	0.0
14	4.0907882267114833E-4	0.0	0.0	12.125778102010623	0.0
15	4.0907882267114833E-4	0.0	0.0	12.536902318795127	0.0
16	4.0907882267114833E-4	0.0	0.0	12.87084699770234	0.0
17	4.0907882267114833E-4	0.0	0.0	13.242972366725528	0.0
18	4.0907882267114833E-4	0.0	0.0	13.634597159629374	0.0
19	4.0907882267114833E-4	0.0	0.0	14.180308309072686	0.0
20	4.0907882267114833E-4	0.0	0.0	14.469254317486074	0.0
21	4.0907882267114833E-4	0.0	0.0	14.729837527527597	0.0
22	4.0907882267114833E-4	0.0	0.0	15.043055546086139	0.0
23	4.0907882267114833E-4	0.0	0.0	15.330774318031512	0.0
24	4.0907882267114833E-4	0.0	0.0	15.576494330849316	0.0
25	4.0907882267114833E-4	0.0	0.0	15.796578737446394	0.0
26	4.0907882267114833E-4	0.0	0.0	16.00289082368021	0.0
27	4.0907882267114833E-4	0.0	0.0	16.242065575335275	0.0
28	5.454384302281978E-4	0.0	0.0	16.454241124694043	0.0
29	5.454384302281978E-4	0.0	0.0	16.679370836770733	0.0
30	5.454384302281978E-4	0.0	0.0	16.944317554254077	0.0
31	5.454384302281978E-4	0.0	0.0	17.18131055218823	0.0
32	5.454384302281978E-4	0.0	0.0	17.42212161913398	0.0
33	5.454384302281978E-4	0.0	0.0	17.648887646501354	0.0
34	5.454384302281978E-4	0.0	0.0	17.87456279700827	0.0
35	5.454384302281978E-4	0.0	0.0	18.108692243183725	0.0
36	5.454384302281978E-4	0.0	0.0	18.348548792876574	0.0
37	5.454384302281978E-4	0.0	0.0	18.5830873178747	0.0
38	5.454384302281978E-4	0.0	0.0	18.823080227175105	0.0
39	6.817980377852472E-4	0.0	0.0	19.063891294120854	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCG	25	3.889419E-5	45.000004	1
TATAGCG	30	2.1644228E-6	45.000004	1
TAGTGCG	25	3.889419E-5	45.000004	1
CGTTTTT	5510	0.0	43.039925	1
TACGGCT	1095	0.0	42.534245	7
CGATGAA	1950	0.0	41.884613	19
TACGGGT	65	0.0	41.53846	4
GATGAAT	2150	0.0	41.23256	20
CCGATGA	1885	0.0	40.941647	18
CGACGGT	55	6.002665E-11	40.90909	28
ACGGCTG	1165	0.0	40.55794	8
ATGAATG	2195	0.0	40.28474	21
CGTATGG	45	1.9266736E-8	40.0	2
TGAATGA	2200	0.0	39.784092	22
TGATACC	1430	0.0	39.65035	4
ACCGATG	1940	0.0	39.43299	17
CGCATCG	40	3.4559343E-7	39.375	21
TCTCGAC	40	3.4559343E-7	39.375	17
CTCGACC	40	3.4559343E-7	39.375	18
GACACGA	75	0.0	39.000004	25
>>END_MODULE