FastQCFastQC Report
Sat 18 Jun 2016
SRR3551975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640738
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150992.3565014093123864No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC54520.8508938130718016No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG38080.5943146808836062No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC28100.4385567892024509No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC18650.29107060920376193No Hit
GAACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT16540.25813983250564193No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT12130.18931294850625374No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC10590.1652781636175785No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC9780.15263649104626228No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG9300.14514512952251934No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT9240.1442087093320515No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT7990.12469995536397094No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC7320.11424326323707976No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT7060.11018544241171899No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTC7010.10940509225299576No Hit
CGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG6420.10019696038006175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA406.8066583E-945.00000410
TGTAACG207.030946E-445.0000041
TTAACGG253.8888662E-545.02
CGTTTTT61000.043.303281
CATACGA900.040.018
TATACGG659.094947E-1238.0769272
ATAGGGA3750.037.84
GTTTTTT71600.037.646652
ATGTGCG301.13935814E-437.4999961
ATCGAGG601.546141E-1037.4999962
TCGGCGT1150.037.1739124
TACGGGT552.744855E-936.8181844
GGTATGC552.744855E-936.8181848
CAAGCGG552.744855E-936.8181842
CCGATGA3500.036.64285718
CGATGAA3500.036.64285719
CCTCGAT1600.036.56250415
TACGGCT3650.036.3698657
TCACGAC250.002106414236.025
CAATGCG250.002106414236.01
Produced by FastQC (version 0.11.5)