##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	640738
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27603170094485	33.0	31.0	34.0	30.0	34.0
2	32.39061987895208	34.0	31.0	34.0	30.0	34.0
3	32.35227659355306	34.0	31.0	34.0	30.0	34.0
4	35.94016431052942	37.0	35.0	37.0	35.0	37.0
5	35.994720150826076	37.0	35.0	37.0	35.0	37.0
6	35.97232097987009	37.0	35.0	37.0	35.0	37.0
7	36.13435912962865	37.0	36.0	37.0	35.0	37.0
8	35.94402860451542	37.0	35.0	37.0	35.0	37.0
9	37.85049583449085	39.0	38.0	39.0	35.0	39.0
10	37.6100496614841	39.0	37.0	39.0	35.0	39.0
11	37.58913003442905	39.0	37.0	39.0	35.0	39.0
12	37.54012248376091	39.0	37.0	39.0	35.0	39.0
13	37.51894534115348	39.0	37.0	39.0	35.0	39.0
14	38.84143596914807	40.0	38.0	41.0	35.0	41.0
15	38.85302416900512	40.0	38.0	41.0	35.0	41.0
16	38.857671934550474	40.0	38.0	41.0	35.0	41.0
17	38.786844232744116	40.0	38.0	41.0	35.0	41.0
18	38.59264941364489	40.0	38.0	41.0	35.0	41.0
19	38.41673663806423	40.0	37.0	41.0	35.0	41.0
20	38.19077064260275	40.0	36.0	41.0	34.0	41.0
21	38.11708217711451	40.0	36.0	41.0	34.0	41.0
22	38.1318417200166	40.0	36.0	41.0	34.0	41.0
23	38.09386207779155	40.0	36.0	41.0	34.0	41.0
24	38.01895938745634	40.0	35.0	41.0	34.0	41.0
25	37.855786608566994	40.0	35.0	41.0	34.0	41.0
26	37.85275572855051	40.0	35.0	41.0	34.0	41.0
27	37.841941636050926	40.0	35.0	41.0	34.0	41.0
28	37.738471887105185	40.0	35.0	41.0	34.0	41.0
29	37.645408887876165	40.0	35.0	41.0	33.0	41.0
30	37.44956128714077	40.0	35.0	41.0	33.0	41.0
31	37.34236146443632	40.0	35.0	41.0	33.0	41.0
32	37.05396277417603	40.0	35.0	41.0	32.0	41.0
33	36.77620962078104	40.0	35.0	41.0	31.0	41.0
34	36.50135624857586	40.0	35.0	41.0	30.0	41.0
35	36.32626752276281	40.0	35.0	41.0	30.0	41.0
36	36.21030124637527	40.0	35.0	41.0	29.0	41.0
37	36.16197103964491	39.0	35.0	41.0	29.0	41.0
38	36.06241864849596	39.0	35.0	41.0	28.0	41.0
39	35.96959287571519	39.0	35.0	41.0	28.0	41.0
40	35.89746042844345	39.0	35.0	41.0	27.0	41.0
41	35.757851102946915	39.0	35.0	41.0	26.0	41.0
42	35.66333665242267	39.0	35.0	41.0	26.0	41.0
43	35.550596343591295	39.0	35.0	41.0	25.0	41.0
44	35.583558334295766	39.0	35.0	41.0	26.0	41.0
45	35.550001716770346	39.0	35.0	41.0	26.0	41.0
46	35.49581732314925	39.0	35.0	41.0	26.0	41.0
47	35.427363446525725	38.0	35.0	41.0	26.0	41.0
48	35.38308637851977	38.0	35.0	41.0	26.0	41.0
49	35.34639119265597	38.0	35.0	41.0	26.0	41.0
50	35.24878655550319	38.0	35.0	40.0	26.0	41.0
51	34.4763475866891	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	9.0
11	8.0
12	12.0
13	12.0
14	25.0
15	25.0
16	57.0
17	110.0
18	205.0
19	386.0
20	657.0
21	1094.0
22	1595.0
23	2347.0
24	3786.0
25	5887.0
26	9132.0
27	11495.0
28	11385.0
29	10102.0
30	10018.0
31	11152.0
32	13302.0
33	17716.0
34	30850.0
35	45331.0
36	48111.0
37	65395.0
38	114317.0
39	225953.0
40	253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.002778046565055	18.317627485805428	21.352877463175275	20.32671700445424
2	37.61240944036408	25.071714179586664	21.47398780780912	15.841888572240133
3	29.155910840312266	24.44509300213192	30.945253754264613	15.453742403291205
4	26.309037391258205	22.37482403103921	33.75279755531903	17.563341022383565
5	25.171286859839746	26.224759574116096	29.533444247102558	19.070509318941596
6	23.515695963092558	33.305656914370616	28.89340104691777	14.285246075619051
7	70.79836064038656	5.850753350043232	18.69828229323062	4.652603716339596
8	70.5929724786106	7.464829618346344	16.69481129572462	5.247386607318436
9	64.20424572914358	7.647431555487579	18.5545417939938	9.59378092137504
10	35.64342991987365	23.47402526461674	24.765660847335415	16.1168839681742
11	27.680268690166653	21.801890944504617	30.68321217096536	19.834628194363376
12	23.644609809313636	19.561817778873735	35.545417939938005	21.24815447187462
13	22.39839060583265	20.17860654432857	38.10231327001052	19.320689579828258
14	19.841339205728396	24.277785928101657	35.01758909257762	20.863285773592327
15	18.957046405863238	22.76671588075001	38.068602143153676	20.207635570233077
16	20.90776573263955	22.567258380180355	34.802992798928734	21.72198308825136
17	20.87280604552875	23.417683983156923	33.362466405925666	22.34704356538866
18	21.3274380480009	23.049826918334794	33.30924652510075	22.313488508563562
19	22.075793850216467	24.31477452562514	31.58451660429068	22.024915019867716
20	22.959150229891158	23.96564586461237	32.574000605551724	20.501203299944752
21	22.45691686773689	25.25540860695011	32.27621898498294	20.011455540330054
22	21.238322059874708	22.394957065134268	32.55027796072654	23.81644291426449
23	21.74211612234642	24.205993713499122	32.52421426542519	21.527675898729278
24	21.88101845059915	23.79428096975675	31.892910987018098	22.431789592626004
25	21.762093086409735	25.55615555812204	30.830230140868807	21.851521214599416
26	20.743267919180695	24.333815069497984	31.964859271652372	22.958057739668945
27	23.153145279349747	22.903901438653556	31.56688069070353	22.376072591293163
28	20.84393308965599	24.23658343972107	32.425421935330824	22.49406153529212
29	22.018828288629674	24.3887517206721	30.698350964044586	22.89406902665364
30	20.81708904419591	24.708539215716876	32.03587113609619	22.438500603991024
31	22.20860944723116	23.678789146265714	28.916343341584234	25.19625806491889
32	22.084845912057656	24.66265462638395	29.0221588231071	24.23034063845129
33	20.735464417593462	24.35878627457713	28.64134794565017	26.264401362179235
34	20.055155149218557	26.145944208085055	28.360109748446323	25.43879089425007
35	20.93991615917895	24.672487038383863	28.6973770870465	25.69021971539069
36	20.641042048387952	26.51957586408173	28.363543289144705	24.475838798385613
37	20.3919542777235	26.02358530319725	29.616473503990708	23.967986915088538
38	20.162063120963637	25.52494155177311	27.76111296661038	26.551882360652872
39	21.67531814875971	24.795470223398645	28.902765248822455	24.626446379019193
40	21.053847282352535	23.97438578639007	30.95789542683593	24.013871504421463
41	19.355337126875572	26.03404199532414	30.31332619573055	24.29729468206974
42	20.320005993089218	25.37168078059987	30.708183376044502	23.60012985026641
43	21.94422681345573	23.155018119730684	30.695541703473182	24.2052133633404
44	22.104822876120974	23.710159222646386	29.59134622887982	24.59367167235282
45	21.447299832380786	23.06683855179496	29.546241989705617	25.93961962611863
46	22.925439103034314	24.36050304492632	29.32118900392984	23.392868848109526
47	20.820210444830806	24.339745730704283	31.96189394104923	22.878149883415684
48	20.631521776451528	24.14855994181709	30.364361096111047	24.85555718562033
49	20.51899528356364	23.361654841760597	32.39732932961679	23.722020545058978
50	20.10603397956731	22.634212423798807	32.63190258732899	24.627851009304898
51	20.60826734172158	22.87799381338394	30.472829768173575	26.040909076720908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	377.0
1	760.0
2	1143.0
3	11531.0
4	21919.0
5	13883.5
6	5848.0
7	5629.5
8	5411.0
9	5278.5
10	5146.0
11	4967.0
12	4788.0
13	4677.5
14	4567.0
15	4238.0
16	3909.0
17	3633.5
18	3358.0
19	3223.5
20	3089.0
21	3103.0
22	3117.0
23	3024.0
24	2931.0
25	3585.5
26	4818.5
27	5397.0
28	6248.0
29	7099.0
30	7976.5
31	8854.0
32	9582.0
33	10310.0
34	11005.5
35	11701.0
36	12982.5
37	14264.0
38	15049.5
39	15835.0
40	18320.0
41	20805.0
42	22759.5
43	24714.0
44	28701.5
45	32689.0
46	46903.5
47	61118.0
48	57898.5
49	54679.0
50	53394.0
51	52109.0
52	46315.0
53	40521.0
54	37102.5
55	33684.0
56	32927.0
57	32170.0
58	30832.0
59	29494.0
60	28414.5
61	27335.0
62	25257.0
63	23179.0
64	21492.0
65	19805.0
66	17027.0
67	14249.0
68	12199.5
69	10150.0
70	8872.5
71	7595.0
72	6343.5
73	5092.0
74	4314.5
75	2760.0
76	1983.0
77	1631.5
78	1280.0
79	993.5
80	707.0
81	529.0
82	351.0
83	236.5
84	122.0
85	85.0
86	48.0
87	28.5
88	9.0
89	7.5
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	640738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.26545586509523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.65090970661225	24.91536538087362
2	7.979570157663899	5.628063584350543
3	3.845403695647811	4.068297429370258
4	2.7143307904674208	3.8288845077799145
5	2.235265389002241	3.9413826461316726
6	1.8956017688755897	4.0109556310847125
7	1.692826885068218	4.1788818261833915
8	1.5086811632043264	4.256346318038616
9	1.2897894194948312	4.093651066361558
>10	6.075436955109188	30.03513054882148
>50	0.06143413296718927	1.5010095910246217
>100	0.03962056398200819	2.5434427746814663
>500	0.007567972895439765	1.82410108011999
>1k	0.002671049257214035	1.9481255102167965
>5k	4.4517487620233915E-4	0.855925560617453
>10k+	4.4517487620233915E-4	2.3704365443438964
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15099	2.3565014093123864	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	5452	0.8508938130718016	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG	3808	0.5943146808836062	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC	2810	0.4385567892024509	No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1865	0.29107060920376193	No Hit
GAACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCT	1654	0.25813983250564193	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTT	1213	0.18931294850625374	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	1059	0.1652781636175785	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCC	978	0.15263649104626228	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	930	0.14514512952251934	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT	924	0.1442087093320515	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCT	799	0.12469995536397094	No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	732	0.11424326323707976	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	706	0.11018544241171899	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTC	701	0.10940509225299576	No Hit
CGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTG	642	0.10019696038006175	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6821009523393336E-4	0.0	0.0	0.11533575345929226	0.0
2	4.6821009523393336E-4	0.0	0.0	0.6122627345342402	0.0
3	4.6821009523393336E-4	0.0	0.0	0.9184721368172326	0.0
4	4.6821009523393336E-4	0.0	0.0	1.5564864265893392	0.0
5	4.6821009523393336E-4	0.0	0.0	3.429170737493328	0.0
6	6.242801269785778E-4	0.0	0.0	4.7791765120845024	0.0
7	6.242801269785778E-4	0.0	0.0	5.7990941695357545	0.0
8	6.242801269785778E-4	0.0	0.0	7.098377183809919	0.0
9	6.242801269785778E-4	0.0	0.0	7.832218473073238	0.0
10	6.242801269785778E-4	0.0	0.0	9.416329295281379	0.0
11	6.242801269785778E-4	0.0	0.0	10.406593646701149	0.0
12	6.242801269785778E-4	0.0	0.0	11.623315614182395	0.0
13	6.242801269785778E-4	0.0	0.0	12.027224856337536	0.0
14	6.242801269785778E-4	0.0	0.0	12.229179477415105	0.0
15	6.242801269785778E-4	0.0	0.0	12.539602770555204	0.0
16	6.242801269785778E-4	0.0	0.0	12.911049446107457	0.0
17	6.242801269785778E-4	0.0	0.0	13.334779582294168	0.0
18	6.242801269785778E-4	0.0	0.0	13.819220960829544	0.0
19	6.242801269785778E-4	0.0	0.0	14.175840983366056	0.0
20	6.242801269785778E-4	0.0	0.0	14.485639996379176	0.0
21	6.242801269785778E-4	0.0	0.0	14.806988191741398	0.0
22	6.242801269785778E-4	0.0	0.0	15.167509965071527	0.0
23	6.242801269785778E-4	0.0	0.0	15.508991194528809	0.0
24	7.803501587232222E-4	0.0	0.0	15.793350792367551	0.0
25	7.803501587232222E-4	0.0	0.0	16.065224787666722	0.0
26	7.803501587232222E-4	0.0	0.0	16.321023569696194	0.0
27	7.803501587232222E-4	0.0	0.0	16.603978537249233	0.0
28	7.803501587232222E-4	0.0	0.0	16.87132650162781	0.0
29	7.803501587232222E-4	0.0	0.0	17.152720768863404	0.0
30	7.803501587232222E-4	0.0	0.0	17.49061238759056	0.0
31	7.803501587232222E-4	0.0	0.0	17.788862218254575	0.0
32	7.803501587232222E-4	0.0	0.0	18.071505045744125	0.0
33	7.803501587232222E-4	0.0	0.0	18.338540870059212	0.0
34	7.803501587232222E-4	0.0	0.0	18.599333893104514	0.0
35	7.803501587232222E-4	0.0	0.0	18.895398743324105	0.0
36	7.803501587232222E-4	0.0	0.0	19.18662542255961	0.0
37	9.364201904678667E-4	0.0	0.0	19.478788521985585	0.0
38	9.364201904678667E-4	0.0	0.0	19.767049870617942	0.0
39	9.364201904678667E-4	0.0	0.0	20.061554020520088	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	40	6.8066583E-9	45.000004	10
TGTAACG	20	7.030946E-4	45.000004	1
TTAACGG	25	3.8888662E-5	45.0	2
CGTTTTT	6100	0.0	43.30328	1
CATACGA	90	0.0	40.0	18
TATACGG	65	9.094947E-12	38.076927	2
ATAGGGA	375	0.0	37.8	4
GTTTTTT	7160	0.0	37.64665	2
ATGTGCG	30	1.13935814E-4	37.499996	1
ATCGAGG	60	1.546141E-10	37.499996	2
TCGGCGT	115	0.0	37.173912	4
TACGGGT	55	2.744855E-9	36.818184	4
GGTATGC	55	2.744855E-9	36.818184	8
CAAGCGG	55	2.744855E-9	36.818184	2
CCGATGA	350	0.0	36.642857	18
CGATGAA	350	0.0	36.642857	19
CCTCGAT	160	0.0	36.562504	15
TACGGCT	365	0.0	36.369865	7
TCACGAC	25	0.0021064142	36.0	25
CAATGCG	25	0.0021064142	36.0	1
>>END_MODULE