FastQCFastQC Report
Sat 18 Jun 2016
SRR3551974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539415
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85361.5824550670633926No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC13340.24730495073366515No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG12070.22376092618855611No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC12010.22264861006831474TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC10000.18538602004022875No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT9760.18093675555926328Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC9490.1759313330181771No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7100.13162407422856243No Hit
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC6900.12791635382775784TruSeq Adapter, Index 15 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6220.1153101044650223No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC253.888044E-545.012
GCGTTAG406.8030204E-945.01
GTTAACG207.029955E-445.01
TAATTCG207.029955E-445.013
TTGCGAG207.029955E-445.01
AGTTACG207.029955E-445.01
CGCACGG253.888044E-545.02
GCTACGA750.045.010
GGTTACG207.029955E-445.01
GCGTAAG406.8030204E-945.01
GCCCGGT253.888044E-545.031
CCCGATC253.888044E-545.041
CGTTTTT35950.043.2475661
CTACGAA800.042.187511
CATACGA603.6379788E-1241.24999618
CGTAAGG556.002665E-1140.9090922
AAGTGCG501.0786607E-940.51
TGCGTAG501.0786607E-940.51
CGAAAGG451.9250365E-840.02
TATTGCG403.4536788E-739.3751
Produced by FastQC (version 0.11.5)