##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551974_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539415
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29235560746364	33.0	31.0	34.0	30.0	34.0
2	32.399297386984045	34.0	31.0	34.0	31.0	34.0
3	32.389507151265725	34.0	31.0	34.0	30.0	34.0
4	35.956402769667136	37.0	35.0	37.0	35.0	37.0
5	36.02812491310031	37.0	35.0	37.0	35.0	37.0
6	36.0189130817645	37.0	35.0	37.0	35.0	37.0
7	36.19709129334557	37.0	36.0	37.0	35.0	37.0
8	36.026888388346634	37.0	36.0	37.0	35.0	37.0
9	37.95076888851812	39.0	38.0	39.0	35.0	39.0
10	37.64315971932557	39.0	37.0	39.0	35.0	39.0
11	37.600152016536434	39.0	37.0	39.0	35.0	39.0
12	37.56666759359677	39.0	37.0	39.0	35.0	39.0
13	37.572090134682945	39.0	37.0	39.0	35.0	39.0
14	38.85849485090329	40.0	38.0	41.0	35.0	41.0
15	38.89178832624232	40.0	38.0	41.0	35.0	41.0
16	38.882274315693856	40.0	38.0	41.0	35.0	41.0
17	38.8456383304135	40.0	38.0	41.0	35.0	41.0
18	38.708465652605135	40.0	38.0	41.0	35.0	41.0
19	38.56633204490049	40.0	37.0	41.0	35.0	41.0
20	38.418716572583264	40.0	37.0	41.0	35.0	41.0
21	38.30648572991111	40.0	37.0	41.0	34.0	41.0
22	38.33499624593309	40.0	37.0	41.0	35.0	41.0
23	38.30723098171167	40.0	37.0	41.0	35.0	41.0
24	38.247440282528295	40.0	36.0	41.0	34.0	41.0
25	38.08308630646163	40.0	36.0	41.0	34.0	41.0
26	38.09637477637811	40.0	36.0	41.0	34.0	41.0
27	38.08797678967029	40.0	36.0	41.0	34.0	41.0
28	38.01302892948843	40.0	36.0	41.0	34.0	41.0
29	37.9456170110212	40.0	36.0	41.0	34.0	41.0
30	37.75960067851283	40.0	36.0	41.0	33.0	41.0
31	37.665734174985865	40.0	36.0	41.0	33.0	41.0
32	37.41323470797067	40.0	35.0	41.0	33.0	41.0
33	37.20038745678188	40.0	35.0	41.0	33.0	41.0
34	37.000862044993184	40.0	35.0	41.0	32.0	41.0
35	36.83049600029662	40.0	35.0	41.0	31.0	41.0
36	36.7446956425016	40.0	35.0	41.0	31.0	41.0
37	36.68511072180047	40.0	35.0	41.0	31.0	41.0
38	36.55244107041888	40.0	35.0	41.0	30.0	41.0
39	36.49096150459294	39.0	35.0	41.0	30.0	41.0
40	36.42433191513028	39.0	35.0	41.0	30.0	41.0
41	36.350060713921565	39.0	35.0	41.0	30.0	41.0
42	36.25065858383619	39.0	35.0	41.0	30.0	41.0
43	36.14819387669976	39.0	35.0	41.0	30.0	41.0
44	36.14415802304348	39.0	35.0	41.0	30.0	41.0
45	36.10562553877812	39.0	35.0	41.0	30.0	41.0
46	36.010446502229264	39.0	35.0	41.0	30.0	41.0
47	35.962107097503775	39.0	35.0	41.0	29.0	41.0
48	35.89883299500384	39.0	35.0	41.0	29.0	41.0
49	35.843702900364285	39.0	35.0	41.0	29.0	41.0
50	35.7428751517848	38.0	35.0	41.0	29.0	41.0
51	34.909110795954874	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	12.0
11	8.0
12	11.0
13	12.0
14	26.0
15	27.0
16	48.0
17	72.0
18	139.0
19	239.0
20	425.0
21	733.0
22	1105.0
23	1757.0
24	2630.0
25	4398.0
26	6242.0
27	7314.0
28	7099.0
29	7045.0
30	7152.0
31	8307.0
32	10211.0
33	13881.0
34	26437.0
35	38782.0
36	38395.0
37	55525.0
38	102164.0
39	198952.0
40	257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.105799801636955	19.192273110684724	22.895173474968253	15.806753612710065
2	35.81824754595256	24.175634715386114	23.926475904452047	16.079641834209284
3	31.292974796770572	22.970440199104587	30.10780197065339	15.628783033471446
4	27.12123318780531	24.18768480668873	30.21143275585588	18.479649249650084
5	25.01839956248899	28.291945904359352	27.78528591158941	18.90436862156225
6	24.702687170360484	34.28250975593931	26.99164835979719	14.023154713903024
7	74.91356376815624	6.570636708285828	14.063939638311876	4.451859885246054
8	75.35126016147122	7.019085490763141	12.998155409100601	4.631498938665035
9	69.37070715497345	8.303995995661968	15.254674045030264	7.0706228043343256
10	37.224215121937654	26.627364830418138	21.575225012281823	14.573195035362382
11	28.282305831317263	24.715108033703178	28.035742424663756	18.966843710315807
12	25.37396994892615	22.34124004708805	32.322608752074004	19.962181251911794
13	24.37029003642835	23.209402778936443	33.82516244450006	18.595144740135147
14	20.716516967455483	26.722467858698774	32.210635595969705	20.350379577876033
15	20.16239815355524	25.13704661531474	34.91893996273741	19.78161526839261
16	22.067981053548753	24.777768508476775	32.71117785007833	20.443072587896147
17	22.41279905082358	24.48967863333426	30.86844081087845	22.22908150496371
18	22.461184802054078	24.381598583650806	31.69303782801739	21.464178786277728
19	22.922239833894125	26.284029921303638	30.206241947294753	20.587488297507488
20	24.259614582464337	25.78793693167598	30.426665925122588	19.525782560737095
21	23.771122419658333	26.550058860061366	30.45280535394826	19.226013366332044
22	22.124894561701105	25.137602773374862	30.123745168376853	22.613757496547183
23	22.30175282481948	26.1092109044057	30.362707748208706	21.226328522566114
24	21.924492274037615	25.37934614350732	30.945190623175105	21.75097095927996
25	21.720382265973324	27.30902922610606	29.144165438484283	21.826423069436334
26	20.795306025972582	27.52296469323248	29.74704077565511	21.934688505139828
27	22.684204184162475	26.065830575716287	30.28632870795213	20.963636532169108
28	20.764346560625864	27.293642186442717	30.50119110517876	21.440820147752657
29	22.013292177636885	26.94511646876709	29.283204953514456	21.75838640008157
30	23.237025295922432	27.155900373552832	29.015692926596405	20.59138140392833
31	24.574585430512684	27.363903488037966	26.55562044066257	21.505890640786777
32	23.285781819193016	28.589119694483838	27.495898334306613	20.629200152016537
33	23.39682804519711	28.15995105809071	26.684092952550447	21.75912794416173
34	22.24613701880741	28.234847010186964	27.64179713207827	21.877218838927355
35	22.986012624787968	27.933965499661674	27.40821074682758	21.671811128722783
36	23.25871546026714	30.63003438910672	25.391952392870053	20.719297757756088
37	24.298360260652743	28.21037605554165	27.450293373376716	20.04097031042889
38	22.044437029003642	29.416497501923377	26.93695948388532	21.602105985187656
39	23.072958668186832	27.799004477072387	27.281221323100024	21.846815531640758
40	22.813789012170595	27.226161675148074	28.698497446307574	21.261551866373757
41	21.152915658630185	28.1193515197019	26.77734212063068	23.950390701037236
42	21.339784766830732	28.616371439429756	27.426563962811564	22.61727983092795
43	22.56110786685576	25.938655765968686	28.383897370299305	23.116338996876244
44	22.788020355385	26.40823855473059	27.55540724673952	23.248333843144888
45	23.180482559810166	25.909179388782295	27.46086037651901	23.449477674888534
46	22.906852794230787	27.573760462723506	27.67553738772559	21.843849355320117
47	21.901875179592707	27.32645551198984	28.83160460869646	21.940064699720992
48	21.437112427351853	26.609753158514316	29.103195128055393	22.849939286078435
49	22.089485831873418	26.096233883002885	29.647488482893504	22.166791802230193
50	21.23986170202905	25.720271034361296	29.394065793498513	23.645801470111138
51	21.245979440690377	25.580304589230927	28.294541308639914	24.87917466143878
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	595.0
1	836.5
2	1078.0
3	6659.5
4	12241.0
5	7742.0
6	3243.0
7	3154.0
8	3065.0
9	3133.0
10	3201.0
11	3118.5
12	3036.0
13	3137.0
14	3238.0
15	3084.0
16	2930.0
17	2756.0
18	2582.0
19	2620.0
20	2658.0
21	2613.0
22	2568.0
23	2720.0
24	2872.0
25	3437.5
26	4526.5
27	5050.0
28	5970.0
29	6890.0
30	7769.5
31	8649.0
32	9717.0
33	10785.0
34	11634.0
35	12483.0
36	13960.0
37	15437.0
38	16070.0
39	16703.0
40	19445.0
41	22187.0
42	23992.0
43	25797.0
44	28886.0
45	31975.0
46	38066.5
47	44158.0
48	47422.5
49	50687.0
50	48781.5
51	46876.0
52	41732.5
53	36589.0
54	33612.0
55	30635.0
56	27565.0
57	24495.0
58	23403.5
59	22312.0
60	21519.0
61	20726.0
62	18960.0
63	17194.0
64	15354.5
65	13515.0
66	11624.5
67	9734.0
68	8364.5
69	6995.0
70	5858.5
71	4722.0
72	3800.5
73	2879.0
74	2447.0
75	1735.5
76	1456.0
77	1019.0
78	582.0
79	440.5
80	299.0
81	224.0
82	149.0
83	104.5
84	60.0
85	60.5
86	61.0
87	34.0
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	539415.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.04114100914457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9055012536834	26.636385920355142
2	8.678137007040819	6.255399191348679
3	3.3891633411878974	3.664479416483296
4	2.076141020037009	2.993059650320923
5	1.4734072249953216	2.655163877997439
6	1.1906851315114682	2.5748190433378
7	1.0065704712670414	2.539456380340358
8	0.8599880187921696	2.4795959561170764
9	0.7546941510190147	2.448003448408766
>10	6.5524062808921855	39.66011679646789
>50	0.06570719016373817	1.6297430556291386
>100	0.041390356008637004	3.0539967694606136
>500	0.0041390356008637	1.1203084986518113
>1k	0.0015521383503238875	0.6977687087142274
>5k	5.173794501079625E-4	1.591703286366821
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8536	1.5824550670633926	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC	1334	0.24730495073366515	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	1207	0.22376092618855611	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1201	0.22264861006831474	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	1000	0.18538602004022875	No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT	976	0.18093675555926328	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	949	0.1759313330181771	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	710	0.13162407422856243	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	690	0.12791635382775784	TruSeq Adapter, Index 15 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	622	0.1153101044650223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8538602004022876E-4	0.0	0.0	0.1876106522807115	0.0
2	1.8538602004022876E-4	0.0	0.0	0.6877821343492487	0.0
3	1.8538602004022876E-4	0.0	0.0	0.9806920460128101	0.0
4	1.8538602004022876E-4	0.0	0.0	1.3640703354560033	0.0
5	1.8538602004022876E-4	0.0	0.0	2.441719269949853	0.0
6	1.8538602004022876E-4	0.0	0.0	3.2531538796659345	0.0
7	1.8538602004022876E-4	0.0	0.0	3.9299982388328094	0.0
8	1.8538602004022876E-4	0.0	0.0	4.932751221230407	0.0
9	1.8538602004022876E-4	0.0	0.0	5.38082923166764	0.0
10	1.8538602004022876E-4	0.0	0.0	6.504268513111426	0.0
11	1.8538602004022876E-4	0.0	0.0	7.8603672497057	0.0
12	1.8538602004022876E-4	0.0	0.0	9.094481985113502	0.0
13	1.8538602004022876E-4	0.0	0.0	9.559430123374396	0.0
14	1.8538602004022876E-4	0.0	0.0	9.77355097652086	0.0
15	1.8538602004022876E-4	0.0	0.0	10.114290481354802	0.0
16	1.8538602004022876E-4	0.0	0.0	10.700851848762085	0.0
17	1.8538602004022876E-4	0.0	0.0	11.404762566854833	0.0
18	3.707720400804575E-4	0.0	0.0	12.167255267280295	0.0
19	3.707720400804575E-4	0.0	0.0	12.687263053493137	0.0
20	3.707720400804575E-4	0.0	0.0	13.117544006006508	0.0
21	3.707720400804575E-4	0.0	0.0	13.616232399914722	0.0
22	3.707720400804575E-4	0.0	0.0	14.167941195554443	0.0
23	3.707720400804575E-4	0.0	0.0	14.720391535274326	0.0
24	3.707720400804575E-4	0.0	0.0	15.184412743435017	0.0
25	3.707720400804575E-4	0.0	0.0	15.577987263980424	0.0
26	3.707720400804575E-4	0.0	0.0	15.953579340581927	0.0
27	3.707720400804575E-4	0.0	0.0	16.334918383804677	0.0
28	3.707720400804575E-4	0.0	0.0	16.720150533448273	0.0
29	3.707720400804575E-4	0.0	0.0	17.100933418610904	0.0
30	3.707720400804575E-4	0.0	0.0	17.55271914944894	0.0
31	3.707720400804575E-4	0.0	0.0	17.976140819220824	0.0
32	3.707720400804575E-4	0.0	0.0	18.377316166587878	0.0
33	3.707720400804575E-4	0.0	0.0	18.77404224947397	0.0
34	3.707720400804575E-4	0.0	0.0	19.157976696977283	0.0
35	3.707720400804575E-4	0.0	0.0	19.57046059156679	0.0
36	3.707720400804575E-4	0.0	0.0	19.96755744649296	0.0
37	5.561580601206863E-4	0.0	0.0	20.35538500041712	0.0
38	5.561580601206863E-4	0.0	0.0	20.763048858485583	0.0
39	5.561580601206863E-4	0.0	0.0	21.18461666805706	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	25	3.888044E-5	45.0	12
GCGTTAG	40	6.8030204E-9	45.0	1
GTTAACG	20	7.029955E-4	45.0	1
TAATTCG	20	7.029955E-4	45.0	13
TTGCGAG	20	7.029955E-4	45.0	1
AGTTACG	20	7.029955E-4	45.0	1
CGCACGG	25	3.888044E-5	45.0	2
GCTACGA	75	0.0	45.0	10
GGTTACG	20	7.029955E-4	45.0	1
GCGTAAG	40	6.8030204E-9	45.0	1
GCCCGGT	25	3.888044E-5	45.0	31
CCCGATC	25	3.888044E-5	45.0	41
CGTTTTT	3595	0.0	43.247566	1
CTACGAA	80	0.0	42.1875	11
CATACGA	60	3.6379788E-12	41.249996	18
CGTAAGG	55	6.002665E-11	40.909092	2
AAGTGCG	50	1.0786607E-9	40.5	1
TGCGTAG	50	1.0786607E-9	40.5	1
CGAAAGG	45	1.9250365E-8	40.0	2
TATTGCG	40	3.4536788E-7	39.375	1
>>END_MODULE