FastQCFastQC Report
Sat 18 Jun 2016
SRR3551972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414216
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.6238291133128608No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC14380.3471618672383491No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG13910.33581513027019716No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC12910.3116731367209379TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC11430.27594298626803404No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC7460.18009927187747457No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6640.1603028371670819No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT6170.14895610019893005TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC5140.12408984684319291TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC4240.10236205264885954TruSeq Adapter, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA207.028056E-445.06
TTAGGCG453.8380676E-1045.022
GGTACGA207.028056E-445.09
TGTAGCG253.8864706E-545.01
GCGTGCT207.028056E-445.011
AATACGG253.8864706E-545.02
AGTACGG253.8864706E-545.02
GCGTACG207.028056E-445.01
CGCACGG302.1621272E-644.9999962
CGTTTTT13150.042.091251
TGCGATG556.002665E-1140.9090921
CGAGATA1700.039.70588319
CGAATAT800.039.37514
GCTACGA800.039.37510
TAGGGCG1050.038.571435
GCATAAG356.2386152E-638.5714261
CGTGCGG356.2386152E-638.5714262
ACGGGCC356.2386152E-638.5714265
AACGGGC356.2386152E-638.5714264
GCGATAT356.2386152E-638.5714269
Produced by FastQC (version 0.11.5)