##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551972_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	414216
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.282784827239894	33.0	31.0	34.0	30.0	34.0
2	32.36827162639782	34.0	31.0	34.0	30.0	34.0
3	32.3660071074029	34.0	31.0	34.0	30.0	34.0
4	35.92463111033857	37.0	35.0	37.0	35.0	37.0
5	35.99206935511907	37.0	35.0	37.0	35.0	37.0
6	35.98190799003419	37.0	35.0	37.0	35.0	37.0
7	36.19196747590629	37.0	36.0	37.0	35.0	37.0
8	36.03355254263476	37.0	36.0	37.0	35.0	37.0
9	37.93620478204608	39.0	38.0	39.0	35.0	39.0
10	37.580805666608725	39.0	37.0	39.0	35.0	39.0
11	37.530402012476586	39.0	37.0	39.0	35.0	39.0
12	37.53346080305927	39.0	37.0	39.0	35.0	39.0
13	37.55520308244974	39.0	37.0	39.0	35.0	39.0
14	38.82727369295247	40.0	38.0	41.0	35.0	41.0
15	38.86799157927265	40.0	38.0	41.0	35.0	41.0
16	38.86634750951195	40.0	38.0	41.0	35.0	41.0
17	38.81987900032833	40.0	38.0	41.0	35.0	41.0
18	38.743042277459104	40.0	38.0	41.0	35.0	41.0
19	38.681963033779475	40.0	38.0	41.0	35.0	41.0
20	38.60624408521158	40.0	38.0	41.0	35.0	41.0
21	38.51441277014891	40.0	38.0	41.0	34.0	41.0
22	38.54175840624215	40.0	38.0	41.0	35.0	41.0
23	38.50283668424204	40.0	38.0	41.0	35.0	41.0
24	38.45479653127837	40.0	38.0	41.0	34.0	41.0
25	38.282294744770844	40.0	37.0	41.0	34.0	41.0
26	38.34068939876779	40.0	37.0	41.0	34.0	41.0
27	38.349788516136506	40.0	37.0	41.0	34.0	41.0
28	38.27361811228924	40.0	37.0	41.0	34.0	41.0
29	38.254780114722756	40.0	37.0	41.0	34.0	41.0
30	38.12012814570176	40.0	37.0	41.0	34.0	41.0
31	38.075301292079494	40.0	37.0	41.0	34.0	41.0
32	37.983824864321996	40.0	37.0	41.0	34.0	41.0
33	37.89214805801804	40.0	37.0	41.0	34.0	41.0
34	37.81150414276609	40.0	37.0	41.0	33.0	41.0
35	37.73163277130772	40.0	37.0	41.0	33.0	41.0
36	37.633012727659	40.0	37.0	41.0	33.0	41.0
37	37.6117919153292	40.0	37.0	41.0	33.0	41.0
38	37.52559775576028	40.0	37.0	41.0	33.0	41.0
39	37.50270873167623	40.0	36.0	41.0	33.0	41.0
40	37.44102835235722	40.0	36.0	41.0	33.0	41.0
41	37.36836819437202	40.0	36.0	41.0	33.0	41.0
42	37.282470981323755	40.0	36.0	41.0	33.0	41.0
43	37.14688954555111	40.0	36.0	41.0	32.0	41.0
44	37.20507899260289	40.0	35.0	41.0	33.0	41.0
45	37.177052552291556	40.0	35.0	41.0	33.0	41.0
46	37.11551702493385	40.0	35.0	41.0	32.0	41.0
47	37.053962183981305	39.0	35.0	41.0	32.0	41.0
48	37.02149120265755	39.0	35.0	41.0	32.0	41.0
49	36.98956341618865	39.0	35.0	41.0	32.0	41.0
50	36.90240116267841	39.0	35.0	41.0	32.0	41.0
51	36.13215327268865	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	8.0
11	7.0
12	4.0
13	8.0
14	15.0
15	12.0
16	30.0
17	54.0
18	78.0
19	149.0
20	261.0
21	433.0
22	698.0
23	1012.0
24	1562.0
25	2020.0
26	2836.0
27	3537.0
28	3741.0
29	3967.0
30	4568.0
31	5692.0
32	7357.0
33	10083.0
34	18419.0
35	26596.0
36	26193.0
37	39758.0
38	78629.0
39	176261.0
40	224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.256696989010564	19.826129362458232	24.892568128705797	13.024605519825405
2	32.63466404001777	25.460629236919868	25.13929930277923	16.765407420283136
3	30.916478359116983	24.86890897502752	27.647169592676285	16.567443073179213
4	28.157048496436644	24.94857755374008	28.150771578113837	18.743602371709446
5	26.09097668849103	28.848716611622923	24.96644262896653	20.09386407091952
6	25.947331826872936	35.06093439171833	24.197037294551635	14.794696486857099
7	79.31176004789772	5.561349634007378	10.88948761032891	4.237402707765996
8	79.64902369778086	6.57072638430191	9.173474708847557	4.6067752090696645
9	72.61742665662359	8.44559360333739	11.545908414933272	7.391071325105742
10	33.95474824729126	34.272698302335016	18.145363771558802	13.627189678814918
11	26.517807134441934	24.676497286439925	30.030950035730154	18.77474554338799
12	24.805898371863954	22.41101261177743	32.29015779206984	20.49293122428878
13	24.297950827587538	23.358827278521353	33.28215230700891	19.061069586882205
14	20.86833922397976	27.402369778086793	30.503167429553663	21.22612356837978
15	19.49997102960774	26.250072425980647	34.0711126561987	20.178843888212914
16	21.533209726326362	25.302257759236728	32.009869246962936	21.154663267473975
17	21.570871236263205	25.02052069451687	30.234467041350406	23.174141027869517
18	21.720310176333122	24.51885006856326	31.694091971338622	22.066747783764992
19	21.948210595438127	26.524566892635725	29.46385460725805	22.063367904668098
20	23.497160901558605	26.094356567587923	30.242675319157154	20.16580721169631
21	23.096403808640904	26.695009367093498	30.030467195859167	20.178119628406435
22	21.85985089904784	24.006074125576994	29.78108040249532	24.35299457287985
23	20.565115785001062	26.842758367614966	30.443053865616005	22.149071981767968
24	21.50713637329316	25.67597581937926	30.425913050196034	22.390974757131545
25	20.383085153639648	28.043581126755125	28.764219634200515	22.809114085404715
26	20.20491724124611	27.43544430924928	29.09810340498677	23.261535044517835
27	21.840054464337445	25.845935453966046	29.13069509627827	23.183314985418235
28	19.682967340711127	26.930876644069762	30.418187612260272	22.967968402958842
29	20.44199161789984	24.88725689012496	29.21012225505533	25.460629236919868
30	21.109759159472354	25.541504915309886	29.672924271394635	23.675811653823125
31	21.25654248025185	26.616547888058406	28.13411360256485	23.992796029124904
32	21.44026305116171	26.603028371670817	27.858894876103292	24.09781370106418
33	21.106379280375457	25.777613612221646	28.804295343492285	24.31171176391062
34	20.31065917299187	25.376132259497457	30.15479846263785	24.15841010487282
35	20.00164165556135	24.612279583598895	29.900100430693165	25.48597833014659
36	21.560731598972517	26.575747918960158	28.62588601116326	23.23763447090407
37	20.514417598547617	26.756812870579598	29.894547772176832	22.834221758695946
38	19.90603936110628	27.164812561562083	29.413397840740096	23.515750236591536
39	22.07495606157174	24.415039496301446	30.09589199837766	23.414112443749154
40	21.222743689282886	23.815110956602354	33.063908685323604	21.898236668791164
41	19.9577032273017	25.232245977943872	30.90875292118122	23.901297873573206
42	21.24616142302567	25.357542924464532	30.152142843347434	23.24415280916237
43	22.084130019120458	25.10622477161674	29.123452498213492	23.68619271104931
44	21.188220638507445	25.415483708982755	29.295584912219713	24.10071074029009
45	21.013191185275314	24.02345636093246	29.635504181393284	25.32784827239894
46	21.799495915174692	25.548747513374664	29.53748768758329	23.114268883867354
47	20.041234524982134	25.182754891167892	31.955549761477105	22.82046082237287
48	20.698862429263958	24.557477258242077	30.895474824729124	23.848185487764837
49	20.64116306468123	24.74795758734573	30.96379666647353	23.647082681499505
50	20.072377696660677	23.87619020028198	31.41525194584468	24.63618015721266
51	19.85171987562045	23.5104389980107	29.740521853332563	26.89731927303629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	278.0
1	473.0
2	668.0
3	2403.0
4	4138.0
5	2738.0
6	1338.0
7	1457.0
8	1576.0
9	1466.0
10	1356.0
11	1262.5
12	1169.0
13	1229.5
14	1290.0
15	1199.5
16	1109.0
17	1083.0
18	1057.0
19	1118.0
20	1179.0
21	1378.5
22	1578.0
23	1799.0
24	2020.0
25	2369.0
26	3386.5
27	4055.0
28	4713.0
29	5371.0
30	6574.5
31	7778.0
32	8745.5
33	9713.0
34	11011.5
35	12310.0
36	13078.0
37	13846.0
38	15023.0
39	16200.0
40	18132.0
41	20064.0
42	21701.0
43	23338.0
44	25346.5
45	27355.0
46	33267.5
47	39180.0
48	40008.0
49	40836.0
50	39244.5
51	37653.0
52	33322.5
53	28992.0
54	26090.0
55	23188.0
56	21072.0
57	18956.0
58	17649.5
59	16343.0
60	15118.0
61	13893.0
62	12616.5
63	11340.0
64	9717.5
65	8095.0
66	6427.5
67	4760.0
68	4008.5
69	3257.0
70	2697.0
71	2137.0
72	1994.5
73	1852.0
74	1451.5
75	810.0
76	569.0
77	447.5
78	326.0
79	236.0
80	146.0
81	127.0
82	108.0
83	64.0
84	20.0
85	13.5
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	414216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.03942158011238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.78569996965766	25.510756830498405
2	8.257582697527987	5.9519700813770555
3	3.7008857249928293	4.001333425885092
4	2.478925912134387	3.5735622405310323
5	1.9795571104595049	3.567104662287959
6	1.721249368644158	3.7219698984641445
7	1.5503022456614464	3.911039734559147
8	1.3346449186048568	3.847986470508417
9	1.2302444996410284	3.99035701522597
>10	6.874329465774943	36.25333549167556
>50	0.05100724499605357	1.2773568883638309
>100	0.029530510260873125	1.8806002285606485
>500	0.0026845918418975565	0.6146115988131972
>1k	0.0033557398023719457	1.8980154332495704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2584	0.6238291133128608	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	1438	0.3471618672383491	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	1391	0.33581513027019716	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1291	0.3116731367209379	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	1143	0.27594298626803404	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	746	0.18009927187747457	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	664	0.1603028371670819	No Hit
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	617	0.14895610019893005	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	514	0.12408984684319291	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	424	0.10236205264885954	TruSeq Adapter, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4141993549259324E-4	0.0	0.0	0.15330165903779672	0.0
2	2.4141993549259324E-4	0.0	0.0	0.7645769357050428	0.0
3	2.4141993549259324E-4	0.0	0.0	1.0745601328775325	0.0
4	2.4141993549259324E-4	0.0	0.0	1.552088765281882	0.0
5	2.4141993549259324E-4	0.0	0.0	2.989020221333797	0.0
6	2.4141993549259324E-4	0.0	0.0	3.790775827104699	0.0
7	2.4141993549259324E-4	0.0	0.0	4.528796569905556	0.0
8	2.4141993549259324E-4	0.0	0.0	5.6407767927844406	0.0
9	2.4141993549259324E-4	0.0	0.0	6.1233752438341345	0.0
10	2.4141993549259324E-4	0.0	0.0	7.38624292639589	0.0
11	2.4141993549259324E-4	0.0	0.0	8.621830156246983	0.0
12	2.4141993549259324E-4	0.0	0.0	9.835206752032756	0.0
13	2.4141993549259324E-4	0.0	0.0	10.23041118643413	0.0
14	4.8283987098518647E-4	0.0	0.0	10.397715201730499	0.0
15	4.8283987098518647E-4	0.0	0.0	10.759603685033895	0.0
16	4.8283987098518647E-4	0.0	0.0	11.329837572667401	0.0
17	4.8283987098518647E-4	0.0	0.0	12.04564768140294	0.0
18	4.8283987098518647E-4	0.0	0.0	12.826882592656972	0.0
19	4.8283987098518647E-4	0.0	0.0	13.317689321513413	0.0
20	4.8283987098518647E-4	0.0	0.0	13.756590764238949	0.0
21	4.8283987098518647E-4	0.0	0.0	14.275643625548023	0.0
22	4.8283987098518647E-4	0.0	0.0	14.851430171697858	0.0
23	4.8283987098518647E-4	0.0	0.0	15.439770554493307	0.0
24	4.8283987098518647E-4	0.0	0.0	15.898468431929235	0.0
25	7.242598064777797E-4	0.0	0.0	16.276773470846127	0.0
26	7.242598064777797E-4	0.0	0.0	16.651457210730634	0.0
27	7.242598064777797E-4	0.0	0.0	17.03290070880893	0.0
28	7.242598064777797E-4	0.0	0.0	17.428346563145798	0.0
29	7.242598064777797E-4	0.0	0.0	17.832000695289413	0.0
30	7.242598064777797E-4	0.0	0.0	18.289008633176895	0.0
31	7.242598064777797E-4	0.0	0.0	18.696042644417407	0.0
32	7.242598064777797E-4	0.0	0.0	19.109353573980727	0.0
33	7.242598064777797E-4	0.0	0.0	19.504316588446606	0.0
34	7.242598064777797E-4	0.0	0.0	19.876103289105203	0.0
35	7.242598064777797E-4	0.0	0.0	20.285068659829655	0.0
36	7.242598064777797E-4	0.0	0.0	20.654682581068815	0.0
37	7.242598064777797E-4	0.0	0.0	21.04530003669583	0.0
38	7.242598064777797E-4	0.0	0.0	21.448954168839446	0.0
39	7.242598064777797E-4	0.0	0.0	21.837157425111535	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	20	7.028056E-4	45.0	6
TTAGGCG	45	3.8380676E-10	45.0	22
GGTACGA	20	7.028056E-4	45.0	9
TGTAGCG	25	3.8864706E-5	45.0	1
GCGTGCT	20	7.028056E-4	45.0	11
AATACGG	25	3.8864706E-5	45.0	2
AGTACGG	25	3.8864706E-5	45.0	2
GCGTACG	20	7.028056E-4	45.0	1
CGCACGG	30	2.1621272E-6	44.999996	2
CGTTTTT	1315	0.0	42.09125	1
TGCGATG	55	6.002665E-11	40.909092	1
CGAGATA	170	0.0	39.705883	19
CGAATAT	80	0.0	39.375	14
GCTACGA	80	0.0	39.375	10
TAGGGCG	105	0.0	38.57143	5
GCATAAG	35	6.2386152E-6	38.571426	1
CGTGCGG	35	6.2386152E-6	38.571426	2
ACGGGCC	35	6.2386152E-6	38.571426	5
AACGGGC	35	6.2386152E-6	38.571426	4
GCGATAT	35	6.2386152E-6	38.571426	9
>>END_MODULE