##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382733
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44110385046495	33.0	31.0	34.0	31.0	34.0
2	32.53223526583807	34.0	31.0	34.0	31.0	34.0
3	32.52154373936922	34.0	31.0	34.0	31.0	34.0
4	36.02854470348781	37.0	35.0	37.0	35.0	37.0
5	36.10656515116282	37.0	35.0	37.0	35.0	37.0
6	36.098259099685684	37.0	36.0	37.0	35.0	37.0
7	36.26684921342032	37.0	37.0	37.0	35.0	37.0
8	36.13591198041455	37.0	37.0	37.0	35.0	37.0
9	38.04684989274507	39.0	39.0	39.0	35.0	39.0
10	37.73825356057617	39.0	37.0	39.0	35.0	39.0
11	37.69437963279884	39.0	37.0	39.0	35.0	39.0
12	37.62438566833798	39.0	37.0	39.0	35.0	39.0
13	37.62317856051085	39.0	37.0	39.0	35.0	39.0
14	38.92091876059812	40.0	38.0	41.0	35.0	41.0
15	38.98080123741616	40.0	38.0	41.0	35.0	41.0
16	38.962982026634755	40.0	38.0	41.0	35.0	41.0
17	38.94104244995859	40.0	38.0	41.0	35.0	41.0
18	38.85349055346678	40.0	38.0	41.0	35.0	41.0
19	38.772381790961326	40.0	38.0	41.0	35.0	41.0
20	38.68781369779977	40.0	38.0	41.0	35.0	41.0
21	38.59874115898028	40.0	38.0	41.0	35.0	41.0
22	38.62140709058273	40.0	38.0	41.0	35.0	41.0
23	38.60313064198802	40.0	38.0	41.0	35.0	41.0
24	38.55494300204059	40.0	38.0	41.0	35.0	41.0
25	38.38239451523647	40.0	37.0	41.0	34.0	41.0
26	38.39904842279082	40.0	37.0	41.0	35.0	41.0
27	38.416807539459626	40.0	37.0	41.0	35.0	41.0
28	38.33409191263884	40.0	37.0	41.0	35.0	41.0
29	38.30447596627414	40.0	37.0	41.0	34.0	41.0
30	38.14685172169633	40.0	37.0	41.0	34.0	41.0
31	38.08922408049476	40.0	37.0	41.0	34.0	41.0
32	37.98260667358184	40.0	37.0	41.0	34.0	41.0
33	37.86038308690392	40.0	37.0	41.0	34.0	41.0
34	37.755571116156695	40.0	36.0	41.0	34.0	41.0
35	37.6707548081822	40.0	36.0	41.0	33.0	41.0
36	37.56262459730413	40.0	36.0	41.0	33.0	41.0
37	37.52278481343391	40.0	36.0	41.0	33.0	41.0
38	37.426503593889215	40.0	35.0	41.0	33.0	41.0
39	37.387821274883535	40.0	35.0	41.0	33.0	41.0
40	37.30824621864328	40.0	35.0	41.0	33.0	41.0
41	37.24830364771263	40.0	35.0	41.0	33.0	41.0
42	37.12915792471514	40.0	35.0	41.0	33.0	41.0
43	37.00904808312845	40.0	35.0	41.0	32.0	41.0
44	37.011355174495016	39.0	35.0	41.0	32.0	41.0
45	36.9655033665767	39.0	35.0	41.0	32.0	41.0
46	36.88755868973932	39.0	35.0	41.0	32.0	41.0
47	36.84093349671964	39.0	35.0	41.0	32.0	41.0
48	36.77637413026836	39.0	35.0	41.0	32.0	41.0
49	36.72767699675753	39.0	35.0	41.0	32.0	41.0
50	36.63746266979853	39.0	35.0	41.0	32.0	41.0
51	35.77534991756655	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	8.0
11	2.0
12	8.0
13	9.0
14	13.0
15	14.0
16	31.0
17	33.0
18	59.0
19	120.0
20	189.0
21	300.0
22	499.0
23	793.0
24	1161.0
25	1829.0
26	2497.0
27	3321.0
28	3479.0
29	3606.0
30	4080.0
31	4964.0
32	6396.0
33	8960.0
34	18538.0
35	27298.0
36	25421.0
37	37717.0
38	71737.0
39	159423.0
40	220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.15053313929032	19.974237915204597	24.211395411422583	13.663833534082507
2	33.721419370684	24.04809619238477	24.95499473523318	17.275489701698053
3	32.33193897573504	23.318605921099042	27.56020515607486	16.789249947091054
4	29.009257106128818	25.2677453995344	26.855797644833345	18.86719984950344
5	25.623084500160687	29.56996130461706	25.505247783703	19.301706411519255
6	26.751286144649143	34.442287443204535	23.99871450854773	14.807711903598591
7	79.10606088317444	6.5209950539932535	9.989993023857362	4.3829510389749515
8	80.33694507659386	5.994257093064878	9.209814674982299	4.458983155358958
9	73.5606806834007	8.615928075185574	11.44009008891316	6.383301152500569
10	37.51048380986223	29.609414395936597	17.997925446721343	14.882176347479836
11	29.509083355759756	24.288472642808433	27.00315886009307	19.19928514133874
12	26.669244617004544	21.929386804900545	30.93070103701536	20.47066754107955
13	25.117248839269152	22.86946774905744	32.104365184083946	19.908918227589467
14	22.561420102264503	25.8174758905033	29.84665550135473	21.774448505877466
15	21.026930000809966	24.81155270123036	32.47093927098003	21.690578026979644
16	23.58171362281277	24.11994784876141	31.37618130655052	20.922157221875302
17	23.97990243851458	23.629527634147042	28.7017320168368	23.688837910501576
18	23.698505224268615	24.102180893730093	30.24092513579963	21.95838874620166
19	24.983735397783835	24.875827273843644	28.205302390961844	21.935134937410673
20	24.635450823419983	25.324704167134794	29.247282047798333	20.792562961646894
21	24.916064201414564	25.29831501333828	29.10018211128907	20.685438673958085
22	23.37582596744989	24.125695981271537	28.38271066252453	24.11576738875404
23	22.392111471966096	25.569783635066745	29.156095763887617	22.88200912907954
24	22.696501216252585	25.58049606383562	29.1581859938913	22.56481672602049
25	23.14668450329603	25.72759600034489	28.350312097467423	22.775407398891655
26	21.23229509867192	26.5848515806058	29.0424395074373	23.14041381328498
27	23.496275471412186	25.276367598299597	29.427564385616083	21.799792544672133
28	21.467445974086374	25.955692349496907	29.99244904411169	22.584412632305025
29	23.36955527743884	25.11045559175718	29.15165402512979	22.36833510567419
30	23.773753504401242	25.046442297894355	29.018924419895853	22.16087977780855
31	24.507946793195256	26.133884457310973	27.030070571390503	22.32809817810327
32	24.15260769256897	26.612808406905074	27.571178863594202	21.663405036931753
33	24.080756036192334	25.949421659485854	26.972327967538728	22.997494336783085
34	22.200071590377625	26.417894459061543	28.39368437004387	22.988349580516967
35	24.29840123532593	24.420157133040526	28.235088168514345	23.0463534631192
36	24.10453240248424	27.13902381033253	27.45490981963928	21.301533967543953
37	23.287252471043782	27.751983758912875	27.615073693671565	21.345690076371778
38	22.820347343970862	28.014307624375217	26.85997810484071	22.305366926813207
39	23.838811913265907	24.623954558399717	28.07022127697377	23.467012251360607
40	22.931652091667036	24.500892266932823	29.845610386352888	22.721845255047253
41	21.81311776094562	25.876002330606457	28.182309860921322	24.128570047526605
42	22.32574666934913	27.1332756778224	28.217059934732568	22.323917718095903
43	23.299793851065886	25.173685049368622	28.448814186391036	23.07770691317446
44	23.0999156069636	25.472849218645898	28.047490025683704	23.379745148706803
45	23.34342740239279	24.471106489380325	28.09373636451521	24.091729743711674
46	23.25093472472977	25.54025913626471	28.698857950581736	22.509948188423785
47	22.526931307203714	25.56795468381352	29.672121296047116	22.23299271293565
48	21.94636992368048	26.163670234863467	28.86320228462139	23.02675755683466
49	22.394985538221164	25.40752953103077	29.848745731358417	22.34873919938965
50	21.80789218593641	24.622386885896958	29.662192703529616	23.907528224637016
51	22.39263402946702	24.164887793840613	27.754073988916556	25.688404187775816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	690.0
2	1106.0
3	2738.5
4	4371.0
5	2843.0
6	1315.0
7	1313.5
8	1312.0
9	1268.5
10	1225.0
11	1219.0
12	1213.0
13	1227.5
14	1242.0
15	1142.5
16	1043.0
17	1083.5
18	1124.0
19	1116.5
20	1109.0
21	1272.5
22	1436.0
23	1577.5
24	1719.0
25	2104.5
26	3047.5
27	3605.0
28	4093.5
29	4582.0
30	5737.5
31	6893.0
32	7056.0
33	7219.0
34	8391.0
35	9563.0
36	10216.5
37	10870.0
38	11708.5
39	12547.0
40	14449.5
41	16352.0
42	17559.0
43	18766.0
44	20522.5
45	22279.0
46	25574.0
47	28869.0
48	31525.5
49	34182.0
50	33589.5
51	32997.0
52	30219.0
53	27441.0
54	25148.5
55	22856.0
56	21490.0
57	20124.0
58	19093.5
59	18063.0
60	17142.0
61	16221.0
62	15074.0
63	13927.0
64	12555.5
65	11184.0
66	9301.5
67	7419.0
68	6237.5
69	5056.0
70	4328.5
71	3601.0
72	3237.5
73	2874.0
74	2491.0
75	1589.0
76	1070.0
77	815.5
78	561.0
79	408.5
80	256.0
81	203.0
82	150.0
83	102.5
84	55.0
85	41.5
86	28.0
87	31.5
88	35.0
89	18.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	382733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.56421179940551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.25040675394405	23.57899531283766
2	7.950156272555365	5.3368145794084105
3	3.474666957199283	3.498733730514979
4	2.2463694803798435	3.0159048407675835
5	1.8351229047720594	3.079722692685484
6	1.6647485192996068	3.352558313671123
7	1.5646916610979273	3.6762349619698127
8	1.3410710892311681	3.6009595261611502
9	1.3027397590678642	3.9352889873574277
>10	8.25990507708764	41.27598079292308
>50	0.06872832788749653	1.599302380743961
>100	0.03748817884772537	2.702640148413414
>500	0.0031240149039771146	0.6328005158360797
>1k	7.810037259942786E-4	0.7140632167098099
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2724	0.7117233162544123	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	705	0.18420151907465518	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	641	0.16747967904518293	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	557	0.14553226400650063	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	511	0.13351344148531744	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	497	0.12985553897887037	Illumina Single End Adapter 2 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	495	0.1293329814779494	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	465	0.12149461896413427	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	416	0.10869196019156958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13560367148900146	0.0
2	0.0	0.0	0.0	0.5633169859928462	0.0
3	0.0	0.0	0.0	0.7553568675813165	0.0
4	0.0	0.0	0.0	1.035186409324516	0.0
5	0.0	0.0	0.0	1.8736299195522728	0.0
6	0.0	0.0	0.0	2.420486344266107	0.0
7	0.0	0.0	0.0	2.92501561140534	0.0
8	0.0	0.0	0.0	3.7284477690713893	0.0
9	0.0	0.0	0.0	4.098157200973002	0.0
10	0.0	0.0	0.0	4.955412781233915	0.0
11	0.0	0.0	0.0	6.0935430182398695	0.0
12	0.0	0.0	0.0	6.96490765102565	0.0
13	0.0	0.0	0.0	7.273739134069965	0.0
14	0.0	0.0	0.0	7.410126641810348	0.0
15	0.0	0.0	0.0	7.666441096012102	0.0
16	0.0	0.0	0.0	8.171492920652256	0.0
17	0.0	0.0	0.0	8.80091343051161	0.0
18	0.0	0.0	0.0	9.445749386648133	0.0
19	0.0	0.0	0.0	9.838712627340731	0.0
20	0.0	0.0	0.0	10.219134488011225	0.0
21	0.0	0.0	0.0	10.653118492526122	0.0
22	0.0	0.0	0.0	11.132564999621145	0.0
23	0.0	0.0	0.0	11.591631764180251	0.0
24	0.0	0.0	0.0	11.957944572325877	0.0
25	0.0	0.0	0.0	12.289768585410718	0.0
26	0.0	0.0	0.0	12.59259065719444	0.0
27	0.0	0.0	0.0	12.909783060253492	0.0
28	0.0	0.0	0.0	13.25676124086504	0.0
29	0.0	0.0	0.0	13.586495023946197	0.0
30	0.0	0.0	0.0	13.960384915855178	0.0
31	0.0	0.0	0.0	14.316246573982385	0.0
32	0.0	0.0	0.0	14.655386392080118	0.0
33	0.0	0.0	0.0	15.001319457689826	0.0
34	2.612787504605038E-4	0.0	0.0	15.344117178294006	0.0
35	2.612787504605038E-4	0.0	0.0	15.687176177648649	0.0
36	2.612787504605038E-4	0.0	0.0	16.037028424515263	0.0
37	2.612787504605038E-4	0.0	0.0	16.38165509637267	0.0
38	2.612787504605038E-4	0.0	0.0	16.739868263254017	0.0
39	2.612787504605038E-4	0.0	0.0	17.11454199141438	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	30	2.1616925E-6	45.000004	21
TAACGCC	30	2.1616925E-6	45.000004	12
CATCGTT	30	2.1616925E-6	45.000004	36
ATAACGC	30	2.1616925E-6	45.000004	11
TAGTGCG	30	2.1616925E-6	45.000004	1
TCACGAC	25	3.885913E-5	45.0	25
CTACGAA	55	1.8189894E-12	45.0	11
TATAGCG	20	7.027384E-4	45.0	1
GATAGCG	20	7.027384E-4	45.0	1
CGAATAT	60	3.6379788E-12	41.250004	14
TATTAGT	55	6.002665E-11	40.909092	30
CGTTTTT	1030	0.0	40.41262	1
GCGTTTA	45	1.9224899E-8	40.0	32
GCGAATG	40	3.4501682E-7	39.375	1
TCGACGG	40	3.4501682E-7	39.375	2
AACAGCG	40	3.4501682E-7	39.375	1
TACGTAG	40	3.4501682E-7	39.375	1
TAGGGCA	115	0.0	39.130436	5
TAGGGCG	110	0.0	38.863636	5
TCGTTTA	35	6.237371E-6	38.57143	38
>>END_MODULE