##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551969_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 718074 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33272893880018 33.0 31.0 34.0 30.0 34.0 2 32.424474357795994 34.0 31.0 34.0 31.0 34.0 3 32.400695471497365 34.0 31.0 34.0 30.0 34.0 4 35.96172678581873 37.0 35.0 37.0 35.0 37.0 5 36.01023710648206 37.0 35.0 37.0 35.0 37.0 6 36.000448421750406 37.0 35.0 37.0 35.0 37.0 7 36.1388519846144 37.0 36.0 37.0 35.0 37.0 8 35.97735748683284 37.0 35.0 37.0 35.0 37.0 9 37.863976414687066 39.0 38.0 39.0 35.0 39.0 10 37.609705963452235 39.0 37.0 39.0 35.0 39.0 11 37.580047460289606 39.0 37.0 39.0 35.0 39.0 12 37.50009887560335 39.0 37.0 39.0 35.0 39.0 13 37.48215225728825 39.0 37.0 39.0 35.0 39.0 14 38.799299236568935 40.0 38.0 41.0 35.0 41.0 15 38.838745031849086 40.0 38.0 41.0 35.0 41.0 16 38.840755966655244 40.0 38.0 41.0 35.0 41.0 17 38.75667688845439 40.0 38.0 41.0 35.0 41.0 18 38.62719858956041 40.0 38.0 41.0 35.0 41.0 19 38.4911541150355 40.0 37.0 41.0 35.0 41.0 20 38.32553747942413 40.0 37.0 41.0 34.0 41.0 21 38.248825329979915 40.0 37.0 41.0 34.0 41.0 22 38.26694323983322 40.0 37.0 41.0 34.0 41.0 23 38.220991151329805 40.0 36.0 41.0 34.0 41.0 24 38.172095076552 40.0 36.0 41.0 34.0 41.0 25 38.00349128362815 40.0 36.0 41.0 34.0 41.0 26 37.99411202745121 40.0 36.0 41.0 34.0 41.0 27 37.97664446839741 40.0 36.0 41.0 34.0 41.0 28 37.868670916925 40.0 36.0 41.0 34.0 41.0 29 37.770330077401496 40.0 35.0 41.0 33.0 41.0 30 37.592749493784765 40.0 35.0 41.0 33.0 41.0 31 37.521251291649605 40.0 35.0 41.0 33.0 41.0 32 37.320528246392435 40.0 35.0 41.0 33.0 41.0 33 37.12720555263107 40.0 35.0 41.0 32.0 41.0 34 36.92071987009695 40.0 35.0 41.0 31.0 41.0 35 36.7819403014174 40.0 35.0 41.0 31.0 41.0 36 36.68822015558285 40.0 35.0 41.0 31.0 41.0 37 36.64059135966488 40.0 35.0 41.0 31.0 41.0 38 36.51731854934171 40.0 35.0 41.0 30.0 41.0 39 36.45846946136471 39.0 35.0 41.0 30.0 41.0 40 36.38199962677941 39.0 35.0 41.0 30.0 41.0 41 36.276656723401764 39.0 35.0 41.0 30.0 41.0 42 36.169944880332665 39.0 35.0 41.0 30.0 41.0 43 36.038227257914926 39.0 35.0 41.0 29.0 41.0 44 36.05655405988798 39.0 35.0 41.0 29.0 41.0 45 36.01462105576863 39.0 35.0 41.0 29.0 41.0 46 35.92885830708256 39.0 35.0 41.0 29.0 41.0 47 35.861212075635656 39.0 35.0 41.0 29.0 41.0 48 35.81806471199347 38.0 35.0 41.0 29.0 41.0 49 35.74609302105354 38.0 35.0 41.0 29.0 41.0 50 35.63484404114339 38.0 35.0 41.0 29.0 41.0 51 34.86186242643516 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 7.0 9 11.0 10 18.0 11 12.0 12 12.0 13 11.0 14 26.0 15 40.0 16 47.0 17 94.0 18 205.0 19 389.0 20 668.0 21 1251.0 22 2004.0 23 2629.0 24 3871.0 25 5602.0 26 8076.0 27 9868.0 28 9719.0 29 9713.0 30 9840.0 31 11921.0 32 14392.0 33 19314.0 34 36757.0 35 52634.0 36 51289.0 37 72174.0 38 128487.0 39 266661.0 40 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00688508426708 18.261488370279384 21.714475109807623 16.017151435645907 2 35.45999437383891 24.64175001462245 22.73623609822943 17.16201951330921 3 31.213635363486215 24.224383559354607 28.318390583700285 16.243590493458893 4 27.44006885084267 23.762731974698987 30.052055916242615 18.745143258215727 5 26.050239947414887 27.702019569013782 26.266930706306034 19.980809777265296 6 25.168018894988535 34.84376262056556 25.535390502928667 14.452827981517224 7 74.25613516155717 6.598762801605405 14.485554413611968 4.659547623225461 8 74.89353464963222 7.837214548918357 12.484507167784937 4.784743633664497 9 68.55714035043742 8.280344365622485 14.652668109414908 8.509847174525188 10 38.270150430178504 25.026668560621886 21.091558808702167 15.611622200497441 11 30.205104209315476 23.80158590897317 26.658812322963925 19.334497558747426 12 26.294782988939858 21.068580675529265 32.07761317078741 20.55902316474347 13 25.085576138392423 22.012633795402703 32.93393160036431 19.967858465840568 14 20.712210719229496 26.2269626807265 31.31181465977044 21.749011940273565 15 20.441486532028733 23.39800633360907 35.047641329445156 21.112865804917043 16 22.596835423647146 23.348011486281358 31.920804819558985 22.13434827051251 17 22.787762821102003 23.78125374265048 30.464269699223202 22.966713737024318 18 23.596453847375063 23.24022315248846 30.451875433451146 22.71144756668533 19 24.34693360294343 24.80245768541961 29.07095926046619 21.779649451170773 20 26.02851516696051 23.522366775569093 29.667972938722194 20.781145118748206 21 25.161334347156423 25.773666780861028 29.031548280539333 20.033450591443223 22 23.415692533081547 23.312499825923233 29.69150811754777 23.580299523447444 23 23.93374499007066 24.856491113729227 29.110648763219388 22.099115132980724 24 22.72843745909196 23.66789495233082 30.211928018560762 23.39173957001646 25 22.632904129657945 26.403824675451276 28.236922656996356 22.726348537894424 26 21.63802059397778 25.3269161674145 29.517013566846874 23.51804967176085 27 23.978726426524286 24.73770112829597 28.97681854516387 22.306753900015877 28 21.807362472391425 26.466770834203718 29.713511420828493 22.012355272576364 29 23.2499714514103 25.58343569047201 29.205764308413894 21.96082854970379 30 23.756465211106377 25.644571450853253 28.966791723415692 21.632171614624678 31 24.2505647050304 25.97629213702209 26.780387536660566 22.992755621286943 32 24.382445263301552 26.243674050306794 28.000735300261532 21.373145386130123 33 24.647459732562382 25.437907513710282 27.204020755521018 22.71061199820631 34 22.91685815110977 26.232672398666434 27.606346978166595 23.244122472057196 35 24.21714196586981 25.09476739166158 27.024512793946027 23.66357784852258 36 23.19510245462167 28.76723011834435 26.190058406236684 21.8476090207973 37 23.736272306196852 27.050276155382313 27.556352130838885 21.65709940758195 38 22.5109111317218 27.091219010854033 26.88274467533987 23.515125182084297 39 23.636700395780935 24.99617031113785 27.910215381701608 23.45691391137961 40 23.961179488464975 23.856594167174972 29.131955759434263 23.050270584925787 41 20.89728913733125 25.422867281088024 28.56014839696187 25.119695184618855 42 21.520762484089385 26.053582221330952 28.56878260457836 23.85687269000131 43 23.28757203296596 24.809281494664894 28.783523703685137 23.119622768684007 44 23.17435250405947 24.969710642635718 27.933750560527187 23.922186292777624 45 22.691950968841653 24.08985703423324 27.866208775140166 25.35198322178494 46 23.16376863665862 26.364135172698077 27.22825224141244 23.243843949230858 47 22.16094720042781 25.048950386728947 29.901235805780463 22.888866607062784 48 21.894261594208952 24.69647974999791 29.611154282149194 23.79810437364394 49 22.426518715341317 24.388572765480994 30.187000225603487 22.9979082935742 50 21.238201076769247 24.286076365388524 30.1571982831853 24.31852427465693 51 21.8002601403198 23.507326542946828 29.127360132799684 25.56505318393369 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 525.0 1 1010.0 2 1495.0 3 8675.0 4 15855.0 5 10091.5 6 4328.0 7 4213.0 8 4098.0 9 3991.5 10 3885.0 11 3824.0 12 3763.0 13 3583.0 14 3403.0 15 3305.5 16 3208.0 17 3063.5 18 2919.0 19 2814.0 20 2709.0 21 2837.0 22 2965.0 23 3066.5 24 3168.0 25 3907.0 26 5596.0 27 6546.0 28 6966.5 29 7387.0 30 9007.0 31 10627.0 32 11823.0 33 13019.0 34 14164.5 35 15310.0 36 16517.5 37 17725.0 38 18835.5 39 19946.0 40 22316.0 41 24686.0 42 27516.0 43 30346.0 44 33530.5 45 36715.0 46 46817.5 47 56920.0 48 56224.5 49 55529.0 50 55821.0 51 56113.0 52 52236.5 53 48360.0 54 45302.5 55 42245.0 56 40755.5 57 39266.0 58 37755.5 59 36245.0 60 35419.0 61 34593.0 62 32996.0 63 31399.0 64 28136.0 65 24873.0 66 20905.5 67 16938.0 68 14563.5 69 12189.0 70 10408.5 71 8628.0 72 7551.5 73 6475.0 74 5213.0 75 3231.5 76 2512.0 77 1983.0 78 1454.0 79 1029.0 80 604.0 81 473.5 82 343.0 83 210.5 84 78.0 85 52.0 86 26.0 87 39.0 88 52.0 89 27.0 90 2.0 91 2.0 92 2.0 93 2.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 718074.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.44946702673358 #Duplication Level Percentage of deduplicated Percentage of total 1 72.33885143712763 22.75018323023724 2 7.578687907169932 4.766913908848905 3 3.0921263224511404 2.9173717446126632 4 2.039687003704924 2.565882766715001 5 1.6044534675795266 2.5229603212285343 6 1.3029849280792343 2.458690891917522 7 1.2033393292173484 2.649106638933489 8 1.0563023512947667 2.6576116773844727 9 0.9306693734096634 2.6342150194654206 >10 8.727412296455924 45.70833270343926 >50 0.075023409380703 1.6077940801054582 >100 0.0424239517015636 2.642720229559565 >500 0.0058053828644244925 1.2787355949481767 >1k 0.0017862716505921516 1.4061176031434535 >5k 0.0 0.0 >10k+ 4.465679126480379E-4 1.4333635894608554 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10206 1.4213019828040008 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCG 3190 0.44424390800948094 No Hit GAATCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTC 2884 0.4016299155797314 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGC 2531 0.3524706367310333 No Hit GCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC 1407 0.19594080832894661 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 981 0.13661544631890307 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCC 969 0.13494430936087368 No Hit CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 914 0.12728493163657228 No Hit CTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT 811 0.11294100608015331 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.1778423950734883E-4 0.0 0.0 0.11489066586452093 0.0 2 4.1778423950734883E-4 0.0 0.0 0.47348880477499533 0.0 3 4.1778423950734883E-4 0.0 0.0 0.7007634310669931 0.0 4 4.1778423950734883E-4 0.0 0.0 1.0327626400621663 0.0 5 4.1778423950734883E-4 0.0 0.0 2.074438010567156 0.0 6 4.1778423950734883E-4 0.0 0.0 2.8559730612722367 0.0 7 4.1778423950734883E-4 0.0 0.0 3.417892863409621 0.0 8 4.1778423950734883E-4 0.0 0.0 4.1822987602949 0.0 9 4.1778423950734883E-4 0.0 0.0 4.595765895994006 0.0 10 5.570456526764651E-4 0.0 0.0 5.72656857092723 0.0 11 5.570456526764651E-4 0.0 0.0 6.604193996719001 0.0 12 5.570456526764651E-4 0.0 0.0 7.650464993858572 0.0 13 5.570456526764651E-4 0.0 0.0 8.011430576792922 0.0 14 5.570456526764651E-4 0.0 0.0 8.19762308620003 0.0 15 5.570456526764651E-4 0.0 0.0 8.48436233591524 0.0 16 5.570456526764651E-4 0.0 0.0 8.912869704236611 0.0 17 5.570456526764651E-4 0.0 0.0 9.388029645969635 0.0 18 5.570456526764651E-4 0.0 0.0 9.949253141041174 0.0 19 5.570456526764651E-4 0.0 0.0 10.33904583650153 0.0 20 5.570456526764651E-4 0.0 0.0 10.674248057999593 0.0 21 5.570456526764651E-4 0.0 0.0 11.017109657221958 0.0 22 5.570456526764651E-4 0.0 0.0 11.393951041257587 0.0 23 5.570456526764651E-4 0.0 0.0 11.751992134515385 0.0 24 5.570456526764651E-4 0.0 0.0 12.056278322289904 0.0 25 5.570456526764651E-4 0.0 0.0 12.325470633945805 0.0 26 5.570456526764651E-4 0.0 0.0 12.581850895590149 0.0 27 5.570456526764651E-4 0.0 0.0 12.873603556179447 0.0 28 6.963070658455813E-4 0.0 0.0 13.151151552625496 0.0 29 6.963070658455813E-4 0.0 0.0 13.423407615371119 0.0 30 6.963070658455813E-4 0.0 0.0 13.761255803719393 0.0 31 6.963070658455813E-4 0.0 0.0 14.052312157242847 0.0 32 6.963070658455813E-4 0.0 0.0 14.366068121112866 0.0 33 6.963070658455813E-4 0.0 0.0 14.668543910516187 0.0 34 6.963070658455813E-4 0.0 0.0 14.97060191568 0.0 35 6.963070658455813E-4 0.0 0.0 15.269039124101417 0.0 36 6.963070658455813E-4 0.0 0.0 15.562323660235574 0.0 37 8.355684790146977E-4 0.0 0.0 15.830123357759785 0.0 38 8.355684790146977E-4 0.0 0.0 16.16045142979693 0.0 39 8.355684790146977E-4 0.0 0.0 16.468636937140182 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCATG 20 7.031516E-4 45.0 1 ATCGTTC 20 7.031516E-4 45.0 10 TATAGCG 20 7.031516E-4 45.0 1 CGATCTA 20 7.031516E-4 45.0 10 TTAAGCG 30 2.164359E-6 44.999996 1 ATACCGG 25 3.889339E-5 44.999996 2 CGTTTTT 4325 0.0 41.618496 1 CGATGAA 495 0.0 40.000004 19 CGCATCG 45 1.9264917E-8 40.0 21 CGAATAT 120 0.0 39.374996 14 TACGGCT 355 0.0 39.295776 7 CGGGATA 140 0.0 38.571426 6 CATCGTT 35 6.2450144E-6 38.571426 36 GTTAGCG 65 9.094947E-12 38.076927 1 GTACGAG 65 9.094947E-12 38.076927 1 TACGAAT 125 0.0 37.8 12 TGCCACG 30 1.1394954E-4 37.499996 1 ATAGGGC 205 0.0 37.317074 4 TAGGGAT 310 0.0 36.29032 5 AAGCGTG 25 0.0021065837 35.999996 1 >>END_MODULE