FastQCFastQC Report
Sat 18 Jun 2016
SRR3551967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369788
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.5154304628598008No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG17950.4854132638160243No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC16040.4337620474434No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC14820.400770171016907No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC12890.3485781042110615RNA PCR Primer, Index 40 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC7320.1979512585589581No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT5590.15116769608532457Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC4310.11655326835916795Illumina PCR Primer Index 8 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4240.11466029184289378No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA4060.10979263794390298No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4010.10844051186085No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3930.10627711012796522No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT3890.10519540926152281No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3720.10059818057914265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT351.2089367E-745.00000428
CTACGAA551.8189894E-1245.00000411
GACGGTC351.2089367E-745.00000429
CGGTCTA351.2089367E-745.00000431
ACCGAGT207.027074E-445.019
ATTCGCG207.027074E-445.01
AGATTCG207.027074E-445.01
CGAATAT502.1827873E-1145.014
ATACCGG207.027074E-445.02
TACGTAG253.885656E-545.01
AAGTCGA207.027074E-445.041
TACGCGG302.1614942E-644.9999962
TTGACGG302.1614942E-644.9999962
CGTTTTT9050.041.2707141
CACAACG603.6379788E-1241.24999612
AACGGGC850.039.7058834
GTTAGCG403.4497498E-739.3751
AAGCGCG403.4497498E-739.3751
TAACGGG800.039.3753
CAGATAC403.4497498E-739.37545