Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551967_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 369788 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1906 | 0.5154304628598008 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG | 1795 | 0.4854132638160243 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC | 1604 | 0.4337620474434 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC | 1482 | 0.400770171016907 | No Hit |
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC | 1289 | 0.3485781042110615 | RNA PCR Primer, Index 40 (96% over 27bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC | 732 | 0.1979512585589581 | No Hit |
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT | 559 | 0.15116769608532457 | Illumina PCR Primer Index 8 (95% over 24bp) |
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC | 431 | 0.11655326835916795 | Illumina PCR Primer Index 8 (95% over 23bp) |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 424 | 0.11466029184289378 | No Hit |
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA | 406 | 0.10979263794390298 | No Hit |
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 401 | 0.10844051186085 | No Hit |
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG | 393 | 0.10627711012796522 | No Hit |
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT | 389 | 0.10519540926152281 | No Hit |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 372 | 0.10059818057914265 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGACGGT | 35 | 1.2089367E-7 | 45.000004 | 28 |
CTACGAA | 55 | 1.8189894E-12 | 45.000004 | 11 |
GACGGTC | 35 | 1.2089367E-7 | 45.000004 | 29 |
CGGTCTA | 35 | 1.2089367E-7 | 45.000004 | 31 |
ACCGAGT | 20 | 7.027074E-4 | 45.0 | 19 |
ATTCGCG | 20 | 7.027074E-4 | 45.0 | 1 |
AGATTCG | 20 | 7.027074E-4 | 45.0 | 1 |
CGAATAT | 50 | 2.1827873E-11 | 45.0 | 14 |
ATACCGG | 20 | 7.027074E-4 | 45.0 | 2 |
TACGTAG | 25 | 3.885656E-5 | 45.0 | 1 |
AAGTCGA | 20 | 7.027074E-4 | 45.0 | 41 |
TACGCGG | 30 | 2.1614942E-6 | 44.999996 | 2 |
TTGACGG | 30 | 2.1614942E-6 | 44.999996 | 2 |
CGTTTTT | 905 | 0.0 | 41.270714 | 1 |
CACAACG | 60 | 3.6379788E-12 | 41.249996 | 12 |
AACGGGC | 85 | 0.0 | 39.705883 | 4 |
GTTAGCG | 40 | 3.4497498E-7 | 39.375 | 1 |
AAGCGCG | 40 | 3.4497498E-7 | 39.375 | 1 |
TAACGGG | 80 | 0.0 | 39.375 | 3 |
CAGATAC | 40 | 3.4497498E-7 | 39.375 | 45 |