##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369788
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30311692104665	33.0	31.0	34.0	30.0	34.0
2	32.38600765844213	34.0	31.0	34.0	30.0	34.0
3	32.393106320378166	34.0	31.0	34.0	30.0	34.0
4	35.9408390753621	37.0	35.0	37.0	35.0	37.0
5	36.00509751533311	37.0	35.0	37.0	35.0	37.0
6	35.97889331184354	37.0	35.0	37.0	35.0	37.0
7	36.15139214901511	37.0	36.0	37.0	35.0	37.0
8	35.962502839464776	37.0	35.0	37.0	35.0	37.0
9	37.85976829967441	39.0	38.0	39.0	35.0	39.0
10	37.57227654764351	39.0	37.0	39.0	35.0	39.0
11	37.54395491470789	39.0	37.0	39.0	35.0	39.0
12	37.45598559174446	39.0	37.0	39.0	35.0	39.0
13	37.423932090819605	39.0	37.0	39.0	35.0	39.0
14	38.69905729769489	40.0	38.0	41.0	35.0	41.0
15	38.74081906389607	40.0	38.0	41.0	35.0	41.0
16	38.75727984683116	40.0	38.0	41.0	35.0	41.0
17	38.69585005462589	40.0	38.0	41.0	35.0	41.0
18	38.64047778727271	40.0	38.0	41.0	35.0	41.0
19	38.57351779938776	40.0	38.0	41.0	35.0	41.0
20	38.45648858264735	40.0	37.0	41.0	34.0	41.0
21	38.38466634936775	40.0	37.0	41.0	34.0	41.0
22	38.41023505359828	40.0	37.0	41.0	34.0	41.0
23	38.361463865782554	40.0	37.0	41.0	34.0	41.0
24	38.29820059060867	40.0	37.0	41.0	34.0	41.0
25	38.131986435471134	40.0	37.0	41.0	34.0	41.0
26	38.165535387843846	40.0	37.0	41.0	34.0	41.0
27	38.15108927277251	40.0	37.0	41.0	34.0	41.0
28	38.079399547849036	40.0	37.0	41.0	34.0	41.0
29	38.04619944400576	40.0	37.0	41.0	34.0	41.0
30	37.88432020509048	40.0	36.0	41.0	34.0	41.0
31	37.841625471892	40.0	36.0	41.0	33.0	41.0
32	37.73481021558298	40.0	36.0	41.0	33.0	41.0
33	37.62078001449479	40.0	36.0	41.0	33.0	41.0
34	37.50726091706599	40.0	36.0	41.0	33.0	41.0
35	37.420668058455114	40.0	36.0	41.0	33.0	41.0
36	37.36683180633228	40.0	36.0	41.0	33.0	41.0
37	37.323331205988296	40.0	35.0	41.0	33.0	41.0
38	37.19732387205642	40.0	35.0	41.0	33.0	41.0
39	37.143774270663194	40.0	35.0	41.0	33.0	41.0
40	37.0532250911333	40.0	35.0	41.0	32.0	41.0
41	36.988731381223836	39.0	35.0	41.0	32.0	41.0
42	36.904750830205415	39.0	35.0	41.0	32.0	41.0
43	36.79957435070906	39.0	35.0	41.0	32.0	41.0
44	36.821327355133214	39.0	35.0	41.0	32.0	41.0
45	36.782921565870176	39.0	35.0	41.0	32.0	41.0
46	36.694668296429306	39.0	35.0	41.0	31.0	41.0
47	36.61125834261793	39.0	35.0	41.0	31.0	41.0
48	36.584975715815546	39.0	35.0	41.0	31.0	41.0
49	36.550269343515744	39.0	35.0	41.0	31.0	41.0
50	36.46717849145997	39.0	35.0	41.0	31.0	41.0
51	35.67309377264811	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	11.0
11	10.0
12	4.0
13	16.0
14	13.0
15	21.0
16	25.0
17	44.0
18	97.0
19	146.0
20	280.0
21	480.0
22	777.0
23	1050.0
24	1438.0
25	1926.0
26	2692.0
27	3327.0
28	3454.0
29	3757.0
30	4312.0
31	5294.0
32	7002.0
33	9913.0
34	19063.0
35	27778.0
36	25684.0
37	38751.0
38	70623.0
39	141608.0
40	187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.224258223630834	19.717243393511957	23.272523716291495	12.785974666565709
2	32.07107856393393	26.633909158761238	24.366664142697978	16.928348134606853
3	31.619468452194234	25.577087412247018	25.99408309626056	16.809361039298192
4	28.847339556718985	25.070580981535368	27.62528800285569	18.45679145888996
5	26.96815472649194	29.187534479215117	23.568909753696712	20.275401040596233
6	25.606022910424354	35.59012190768765	23.628673726567655	15.175181455320347
7	77.0308933767456	6.5551072506409085	11.75240948868	4.661589883933497
8	76.99492682293638	8.198751717200125	9.699070818955725	5.107250640907764
9	70.13450950274212	8.849665213581837	12.224842342098716	8.790982941577337
10	38.1940463184311	27.000064902051985	18.712613713803584	16.09327506571333
11	29.89334429456878	23.569450604129933	25.868064945320018	20.669140155981264
12	26.46245957143012	21.71081809036529	30.328999318528453	21.497723019676137
13	24.65277402187199	23.586216967559793	32.01915692234469	19.74185208822352
14	21.594805672439342	27.386772961805146	28.994991724988374	22.02342964076714
15	19.973065648425585	24.63817106017502	33.405627007907235	21.983136283492165
16	21.823585405691908	24.273367442967324	30.669464666241197	23.23358248509957
17	21.889028308111673	24.545956061310807	29.421993142016508	24.143022488561012
18	22.64053998507253	24.623027248044828	29.336538773567554	23.39989399331509
19	23.865025365885316	25.269073090527545	28.107185738855776	22.75871580473136
20	24.496468246671064	25.613053966056228	28.580159442707714	21.310318344564994
21	24.405334948673293	26.445963633216866	28.43331855008816	20.715382868021678
22	22.8082036193711	24.13626185814575	27.646381169751315	25.409153352731835
23	22.746546669983882	26.415946434173094	28.083658745010652	22.75384815083237
24	23.32823131091328	25.606293335640963	27.702629614806323	23.362845738639436
25	22.141875885642584	27.55578872218677	27.002769154218093	23.299566237952558
26	21.232706307397752	25.900515971313293	28.74971605352256	24.117061667766396
27	23.291994331887462	25.637392235551182	28.234015165446145	22.836598267115214
28	20.68428396811146	26.78994450874555	29.51691239304683	23.008859130096162
29	22.279792746113987	25.61440609213928	28.598548357437238	23.5072528043095
30	22.32008610338897	26.639588088310056	27.831081592696354	23.209244215604617
31	24.007269029822492	27.315651129836553	24.839908271766525	23.837171568574426
32	24.250922149988643	27.715339599987022	25.26663926357805	22.76709898644629
33	22.627559574675217	27.195311908444843	25.035966553809207	25.141161963070736
34	21.26759116034052	27.798089716269864	27.20531764145943	23.729001481930187
35	22.33036226162017	25.176317241230112	26.346717578720778	26.146602918428936
36	22.091035944919792	28.906562679156707	25.178210217746383	23.82419115817712
37	22.380120501476522	28.33028654255952	25.996246497993447	23.29334645797051
38	21.769770787586403	29.3094962519065	24.506744404902268	24.41398855560483
39	23.24791502157993	26.72639458284206	25.969474401548997	24.056215994029014
40	21.79654288403085	25.871850898352573	28.16505673521045	24.166549482406136
41	19.964412041494047	26.22908260949517	26.73099181152444	27.075513537486344
42	20.886291604919577	26.28397892846712	27.00466213073437	25.825067335878938
43	22.729509881337414	24.380185403528507	27.13257325819118	25.757731456942896
44	22.7852174759592	25.39779549363419	26.977619609073304	24.839367421333307
45	22.90420457126786	25.124666024857483	26.168507360974395	25.802622042900257
46	23.352840005624845	26.42676344283752	26.300474866680368	23.91992168485727
47	21.029887394939802	26.091706599456987	29.341676852683158	23.536729152920053
48	21.410375674710917	25.3369498198968	27.809447575367507	25.44322693002477
49	21.275433491622227	24.953216437526365	29.557205750321806	24.214144320529602
50	20.974991075967854	24.44454660508183	29.383863186474414	25.196599132475907
51	21.303287288933117	24.130853353813535	26.964098348242775	27.601761009010566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	260.0
1	544.5
2	829.0
3	2008.5
4	3188.0
5	2172.5
6	1157.0
7	1263.5
8	1370.0
9	1280.5
10	1191.0
11	1186.5
12	1182.0
13	1150.5
14	1119.0
15	1054.0
16	989.0
17	1002.0
18	1015.0
19	1004.0
20	993.0
21	1087.5
22	1182.0
23	1347.0
24	1512.0
25	1987.5
26	2942.0
27	3421.0
28	4362.5
29	5304.0
30	5605.5
31	5907.0
32	6279.0
33	6651.0
34	7359.0
35	8067.0
36	8790.0
37	9513.0
38	10179.0
39	10845.0
40	12716.0
41	14587.0
42	16559.0
43	18531.0
44	20295.0
45	22059.0
46	27728.0
47	33397.0
48	33714.0
49	34031.0
50	33132.0
51	32233.0
52	29122.5
53	26012.0
54	23602.0
55	21192.0
56	19873.5
57	18555.0
58	17577.0
59	16599.0
60	16132.0
61	15665.0
62	14433.5
63	13202.0
64	12184.0
65	11166.0
66	9490.5
67	7815.0
68	6749.0
69	5683.0
70	4800.0
71	3917.0
72	3452.5
73	2988.0
74	2416.0
75	1435.0
76	1026.0
77	778.0
78	530.0
79	435.0
80	340.0
81	227.0
82	114.0
83	101.0
84	88.0
85	63.5
86	39.0
87	24.5
88	10.0
89	7.5
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	369788.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.065512542532986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43814707321755	23.621389480647906
2	8.381885984892246	5.543027123270719
3	3.257767582778824	3.231592646070917
4	2.1219163777805075	2.8064901041483012
5	1.6970236129538332	2.80564777795498
6	1.420486983521247	2.818147810207598
7	1.2293040196036493	2.845329723515347
8	1.1022320393433735	2.915669385735202
9	0.9842139570218477	2.928918504639772
>10	8.246556928120947	43.21313401586737
>50	0.06965688751773734	1.5876102576890125
>100	0.04507210368794769	3.1448719321963954
>500	0.0016389855886526433	0.3498216150668229
>1k	0.004097463971631608	2.1883496229896684
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1906	0.5154304628598008	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	1795	0.4854132638160243	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	1604	0.4337620474434	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	1482	0.400770171016907	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1289	0.3485781042110615	RNA PCR Primer, Index 40 (96% over 27bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	732	0.1979512585589581	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	559	0.15116769608532457	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	431	0.11655326835916795	Illumina PCR Primer Index 8 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	424	0.11466029184289378	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	406	0.10979263794390298	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	401	0.10844051186085	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	393	0.10627711012796522	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	389	0.10519540926152281	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	372	0.10059818057914265	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15603534998431534	0.0
2	0.0	0.0	0.0	0.7620582604086665	0.0
3	0.0	0.0	0.0	1.0359990048352028	0.0
4	0.0	0.0	0.0	1.4830118878925223	0.0
5	2.704252166105985E-4	0.0	0.0	2.9073415037805446	0.0
6	2.704252166105985E-4	0.0	0.0	3.5977370817874026	0.0
7	2.704252166105985E-4	0.0	0.0	4.320042835354311	0.0
8	2.704252166105985E-4	0.0	0.0	5.375512455785477	0.0
9	2.704252166105985E-4	0.0	0.0	5.809004078012267	0.0
10	2.704252166105985E-4	0.0	0.0	7.200071392257185	0.0
11	2.704252166105985E-4	0.0	0.0	8.46268672861207	0.0
12	2.704252166105985E-4	0.0	0.0	9.861866799355306	0.0
13	2.704252166105985E-4	0.0	0.0	10.26534122253832	0.0
14	2.704252166105985E-4	0.0	0.0	10.425432950771794	0.0
15	2.704252166105985E-4	0.0	0.0	10.788614016679828	0.0
16	2.704252166105985E-4	0.0	0.0	11.423842850498124	0.0
17	2.704252166105985E-4	0.0	0.0	12.117213105887698	0.0
18	2.704252166105985E-4	0.0	0.0	12.893874327993336	0.0
19	2.704252166105985E-4	0.0	0.0	13.396324380455829	0.0
20	2.704252166105985E-4	0.0	0.0	13.83035685311584	0.0
21	2.704252166105985E-4	0.0	0.0	14.320096920397633	0.0
22	2.704252166105985E-4	0.0	0.0	14.840124611939814	0.0
23	2.704252166105985E-4	0.0	0.0	15.336625309636872	0.0
24	2.704252166105985E-4	0.0	0.0	15.753620993650417	0.0
25	2.704252166105985E-4	0.0	0.0	16.127889493439483	0.0
26	2.704252166105985E-4	0.0	0.0	16.484580354148864	0.0
27	2.704252166105985E-4	0.0	0.0	16.838296537475525	0.0
28	5.40850433221197E-4	0.0	0.0	17.202559304250002	0.0
29	5.40850433221197E-4	0.0	0.0	17.543835927612577	0.0
30	5.40850433221197E-4	0.0	0.0	17.935682066481334	0.0
31	5.40850433221197E-4	0.0	0.0	18.291291226324272	0.0
32	5.40850433221197E-4	0.0	0.0	18.652579315716032	0.0
33	5.40850433221197E-4	0.0	0.0	19.01684208249051	0.0
34	5.40850433221197E-4	0.0	0.0	19.376237195365995	0.0
35	5.40850433221197E-4	0.0	0.0	19.74996484472184	0.0
36	5.40850433221197E-4	0.0	0.0	20.09746124806646	0.0
37	8.112756498317956E-4	0.0	0.0	20.43576319404632	0.0
38	8.112756498317956E-4	0.0	0.0	20.77082003742685	0.0
39	8.112756498317956E-4	0.0	0.0	21.111285385139592	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	35	1.2089367E-7	45.000004	28
CTACGAA	55	1.8189894E-12	45.000004	11
GACGGTC	35	1.2089367E-7	45.000004	29
CGGTCTA	35	1.2089367E-7	45.000004	31
ACCGAGT	20	7.027074E-4	45.0	19
ATTCGCG	20	7.027074E-4	45.0	1
AGATTCG	20	7.027074E-4	45.0	1
CGAATAT	50	2.1827873E-11	45.0	14
ATACCGG	20	7.027074E-4	45.0	2
TACGTAG	25	3.885656E-5	45.0	1
AAGTCGA	20	7.027074E-4	45.0	41
TACGCGG	30	2.1614942E-6	44.999996	2
TTGACGG	30	2.1614942E-6	44.999996	2
CGTTTTT	905	0.0	41.270714	1
CACAACG	60	3.6379788E-12	41.249996	12
AACGGGC	85	0.0	39.705883	4
GTTAGCG	40	3.4497498E-7	39.375	1
AAGCGCG	40	3.4497498E-7	39.375	1
TAACGGG	80	0.0	39.375	3
CAGATAC	40	3.4497498E-7	39.375	45
>>END_MODULE