##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469658
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24389662264882	33.0	31.0	34.0	30.0	34.0
2	32.32485766238412	34.0	31.0	34.0	30.0	34.0
3	32.335850768005656	34.0	31.0	34.0	30.0	34.0
4	35.88960264703252	37.0	35.0	37.0	35.0	37.0
5	35.95987505802094	37.0	35.0	37.0	35.0	37.0
6	35.938751176387925	37.0	35.0	37.0	35.0	37.0
7	36.11416818195368	37.0	35.0	37.0	35.0	37.0
8	35.93606624394772	37.0	35.0	37.0	35.0	37.0
9	37.820482138066424	39.0	38.0	39.0	35.0	39.0
10	37.555640487333335	39.0	37.0	39.0	35.0	39.0
11	37.51671854839053	39.0	37.0	39.0	35.0	39.0
12	37.45024890452201	39.0	37.0	39.0	35.0	39.0
13	37.4110927525987	39.0	37.0	39.0	35.0	39.0
14	38.64548458665667	40.0	38.0	41.0	35.0	41.0
15	38.701836229767196	40.0	38.0	41.0	35.0	41.0
16	38.7124056228149	40.0	38.0	41.0	35.0	41.0
17	38.65277286876834	40.0	38.0	41.0	35.0	41.0
18	38.592486447585266	40.0	38.0	41.0	35.0	41.0
19	38.52982170004557	40.0	38.0	41.0	35.0	41.0
20	38.42206243692219	40.0	37.0	41.0	34.0	41.0
21	38.327263668456624	40.0	37.0	41.0	34.0	41.0
22	38.34608800446282	40.0	37.0	41.0	34.0	41.0
23	38.30396160610487	40.0	37.0	41.0	34.0	41.0
24	38.221825243049196	40.0	37.0	41.0	34.0	41.0
25	38.05919200780142	40.0	37.0	41.0	34.0	41.0
26	38.09629773154082	40.0	37.0	41.0	34.0	41.0
27	38.081080275434466	40.0	37.0	41.0	34.0	41.0
28	37.9835497319326	40.0	36.0	41.0	34.0	41.0
29	37.94905867673924	40.0	36.0	41.0	34.0	41.0
30	37.788201201725514	40.0	36.0	41.0	33.0	41.0
31	37.72263647164532	40.0	36.0	41.0	33.0	41.0
32	37.639646295815254	40.0	36.0	41.0	33.0	41.0
33	37.57547619757355	40.0	36.0	41.0	33.0	41.0
34	37.467286834249606	40.0	36.0	41.0	33.0	41.0
35	37.39134433992395	40.0	36.0	41.0	33.0	41.0
36	37.27558138049389	40.0	35.0	41.0	33.0	41.0
37	37.22128442398511	40.0	35.0	41.0	33.0	41.0
38	37.09401096116749	40.0	35.0	41.0	32.0	41.0
39	37.06627801506628	40.0	35.0	41.0	32.0	41.0
40	36.99284798725881	39.0	35.0	41.0	32.0	41.0
41	36.8846650115616	39.0	35.0	41.0	32.0	41.0
42	36.796926699853934	39.0	35.0	41.0	31.0	41.0
43	36.67282575831776	39.0	35.0	41.0	31.0	41.0
44	36.695159030613766	39.0	35.0	41.0	31.0	41.0
45	36.648759309965975	39.0	35.0	41.0	31.0	41.0
46	36.564157322988216	39.0	35.0	41.0	31.0	41.0
47	36.49167692235627	39.0	35.0	41.0	31.0	41.0
48	36.44713600109016	39.0	35.0	41.0	31.0	41.0
49	36.40923821163485	39.0	35.0	41.0	31.0	41.0
50	36.33625744690818	38.0	35.0	41.0	31.0	41.0
51	35.44907783961947	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	6.0
12	8.0
13	19.0
14	16.0
15	29.0
16	44.0
17	91.0
18	129.0
19	239.0
20	405.0
21	610.0
22	889.0
23	1280.0
24	1840.0
25	2539.0
26	3472.0
27	4202.0
28	4555.0
29	4862.0
30	5800.0
31	7383.0
32	9471.0
33	13357.0
34	25162.0
35	35164.0
36	33423.0
37	50852.0
38	91324.0
39	172245.0
40	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51451907558266	18.934841948822335	23.629747603575368	12.920891372019641
2	32.94333323397025	25.34503830446836	24.394133603600917	17.317494857960472
3	32.21131120943325	24.436079019201205	26.53760821704304	16.815001554322507
4	28.438140093429688	24.793786116706197	27.79448023881207	18.973593551052044
5	26.803759331258064	28.3497779235103	24.132027986321962	20.714434758909675
6	25.890754549054844	34.928820546014336	24.095618513897346	15.084806391033476
7	75.79813396130801	7.076851666531817	11.978929348589824	5.146085023570342
8	75.96676730727465	8.039254095533344	10.186348364128792	5.8076302330632075
9	69.19268914827384	8.90520336074335	12.8039978026564	9.0981096883264
10	39.184044560084146	24.696055427566442	19.424985840760726	16.69491417158869
11	30.646555578740276	23.670415493827424	24.88427749553931	20.798751431892995
12	26.777357140727936	21.450928122165493	30.255632822181244	21.516081914925326
13	24.75077609664905	23.309301662060477	32.220679728653614	19.719242512636857
14	20.886474839138266	27.409945108994204	29.279390535240534	22.424189516626992
15	19.570836651350557	24.56063774065384	34.61774312371981	21.250782484275792
16	22.27003479127365	23.767933262075807	30.915048822760394	23.04698312389015
17	21.593585119384745	25.06355688607455	28.714937252213314	24.627920742327394
18	22.223405116063176	24.040684923923365	30.139803857274867	23.596106102738588
19	24.036213585204553	24.968168326739885	27.795119001486192	23.200499086569376
20	24.97327842813281	25.068454066576106	28.816287596506395	21.141979908784688
21	24.29107989217686	26.090048503379055	28.75773435137909	20.861137253064996
22	22.357119435844805	24.6971200320233	27.286450991998436	25.659309540133457
23	22.992475375698913	25.6688909802452	28.11577786389245	23.22285578016344
24	23.602919571262493	24.541049018647612	28.28866962768653	23.567361782403363
25	22.661383389615423	26.613195133480104	26.536117770803436	24.189303706101033
26	21.80437680184304	25.833904671058516	27.160188903414824	25.201529623683616
27	24.17376048103088	25.247733457111345	27.91456762154589	22.663938440311888
28	21.673643374540625	25.710197633171372	29.175698061142363	23.440460931145644
29	24.137563929497635	24.334728674908124	26.606381664956203	24.92132573063804
30	25.15319658134217	25.457034693330037	26.709009534597516	22.680759190730278
31	25.20131670279224	25.26157331505053	25.544119337901193	23.992990644256032
32	25.97187740866758	25.91949886939007	25.921415157412415	22.187208564529932
33	25.659309540133457	26.080467063267314	24.67987343982217	23.58034995677706
34	22.61709584421004	25.853706313956117	27.155078802021897	24.374119039811948
35	24.698610478262907	24.10860668827104	27.077788518453854	24.1149943150122
36	24.78675972729092	27.19936634742728	26.050871059366603	21.9630028659152
37	24.071345532280937	27.538123485600156	27.667792308445723	20.722738673673184
38	23.873542024196333	26.845917667749724	26.59147720256016	22.689063105493783
39	25.019482261560537	25.719140310608996	26.710925822619863	22.5504516052106
40	23.603132492153865	24.40243751836443	29.46931597034438	22.52511401913733
41	20.745095367267247	26.845917667749724	27.316046995899146	25.09293996908389
42	22.480400631949205	25.217498690536516	28.37213461710436	23.929966060409917
43	23.954451962917698	24.560211898871092	27.825353768061014	23.659982370150196
44	24.37454488159469	23.89973129383509	27.393763121249933	24.331960703320288
45	23.977234498294504	23.300358984622854	26.985380851598396	25.737025665484246
46	24.37092522644137	25.595433272721852	26.760749311200914	23.27289218963586
47	22.640942984043708	24.99520927994413	30.187923978724946	22.175923757287215
48	22.6219930247116	24.546584961823285	28.537999991483165	24.293422021981954
49	22.413969313841136	24.43416273117886	29.854064021053617	23.29780393392639
50	22.114815461463447	23.783263566254593	29.243619825490036	24.85830114679192
51	22.145263148929647	23.920171699406804	26.822283448807433	27.112281702856123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	370.0
1	779.5
2	1189.0
3	2423.0
4	3657.0
5	2461.0
6	1265.0
7	1228.5
8	1192.0
9	1218.5
10	1245.0
11	1272.5
12	1300.0
13	1323.5
14	1347.0
15	1280.5
16	1214.0
17	1223.5
18	1233.0
19	1261.5
20	1290.0
21	1390.0
22	1490.0
23	1685.0
24	1880.0
25	2343.5
26	3547.0
27	4287.0
28	4878.0
29	5469.0
30	6176.5
31	6884.0
32	7633.5
33	8383.0
34	9118.0
35	9853.0
36	11093.0
37	12333.0
38	12545.0
39	12757.0
40	14956.5
41	17156.0
42	19795.0
43	22434.0
44	24657.0
45	26880.0
46	35077.5
47	43275.0
48	43463.5
49	43652.0
50	42867.5
51	42083.0
52	37881.5
53	33680.0
54	30300.0
55	26920.0
56	25981.0
57	25042.0
58	23516.5
59	21991.0
60	21474.0
61	20957.0
62	19147.0
63	17337.0
64	15549.5
65	13762.0
66	12153.0
67	10544.0
68	9157.0
69	7770.0
70	6517.5
71	5265.0
72	4565.0
73	3865.0
74	3283.0
75	2129.0
76	1557.0
77	1158.5
78	760.0
79	547.0
80	334.0
81	235.0
82	136.0
83	91.0
84	46.0
85	45.0
86	44.0
87	29.5
88	15.0
89	9.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469658.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.271965112773835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27422470371552	23.324312581910835
2	8.807767856351651	5.684879539631825
3	3.268425363103407	3.164355278753348
4	1.8712739370904512	2.415587488569039
5	1.3855091335661003	2.235655121093734
6	1.1900699847224299	2.304353821723292
7	1.0478251858637402	2.3670764489736262
8	0.940583128833084	2.4283572735491954
9	0.8060631834014594	2.341191864008098
>10	8.250716910910416	45.26763357261915
>50	0.09730449591988682	2.1253008438262952
>100	0.05295482771150304	3.293158891187674
>500	0.00330967673196894	0.7698848367825879
>1k	0.003971612078362728	2.2782524373713375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCG	2350	0.5003640947242461	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2158	0.4594832835808184	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTC	1840	0.3917744401245161	No Hit
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1677	0.3570683348308769	RNA PCR Primer, Index 23 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGC	1615	0.3438672395658117	No Hit
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	1025	0.21824391365632012	RNA PCR Primer, Index 30 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	973	0.2071720273049751	RNA PCR Primer, Index 30 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	843	0.17949231142661257	No Hit
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	617	0.1313721899765361	RNA PCR Primer, Index 23 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	609	0.12966882284555994	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	562	0.11966154095107505	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	485	0.10326663231542953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1292089137201964E-4	0.0	0.0	0.21313381226339165	0.0
2	2.1292089137201964E-4	0.0	0.0	1.0254270128476466	0.0
3	2.1292089137201964E-4	0.0	0.0	1.3688684106307143	0.0
4	2.1292089137201964E-4	0.0	0.0	1.9369413488112626	0.0
5	2.1292089137201964E-4	0.0	0.0	3.57813557950679	0.0
6	2.1292089137201964E-4	0.0	0.0	4.396603485940833	0.0
7	2.1292089137201964E-4	0.0	0.0	5.22486575337799	0.0
8	2.1292089137201964E-4	0.0	0.0	6.392736842553518	0.0
9	2.1292089137201964E-4	0.0	0.0	6.892036332820903	0.0
10	2.1292089137201964E-4	0.0	0.0	8.361190483287839	0.0
11	2.1292089137201964E-4	0.0	0.0	9.69812076021275	0.0
12	2.1292089137201964E-4	0.0	0.0	11.035689799811777	0.0
13	2.1292089137201964E-4	0.0	0.0	11.475158519603626	0.0
14	2.1292089137201964E-4	0.0	0.0	11.647837362506335	0.0
15	2.1292089137201964E-4	0.0	0.0	12.024281498452066	0.0
16	2.1292089137201964E-4	0.0	0.0	12.64153916253955	0.0
17	2.1292089137201964E-4	0.0	0.0	13.371006136380089	0.0
18	2.1292089137201964E-4	0.0	0.0	14.15647130465147	0.0
19	2.1292089137201964E-4	0.0	0.0	14.69643868517091	0.0
20	2.1292089137201964E-4	0.0	0.0	15.181898317499117	0.0
21	2.1292089137201964E-4	0.0	0.0	15.731021296347555	0.0
22	2.1292089137201964E-4	0.0	0.0	16.301223443441824	0.0
23	2.1292089137201964E-4	0.0	0.0	16.878877821734115	0.0
24	2.1292089137201964E-4	0.0	0.0	17.35518185573332	0.0
25	2.1292089137201964E-4	0.0	0.0	17.777403983324035	0.0
26	2.1292089137201964E-4	0.0	0.0	18.159809904228183	0.0
27	2.1292089137201964E-4	0.0	0.0	18.572024749924413	0.0
28	6.38762674116059E-4	0.0	0.0	18.98402667472927	0.0
29	6.38762674116059E-4	0.0	0.0	19.398583650230595	0.0
30	6.38762674116059E-4	0.0	0.0	19.842523708741254	0.0
31	6.38762674116059E-4	0.0	0.0	20.23749196223635	0.0
32	6.38762674116059E-4	0.0	0.0	20.64353210208279	0.0
33	6.38762674116059E-4	0.0	0.0	21.02913183635752	0.0
34	6.38762674116059E-4	0.0	0.0	21.430487716593777	0.0
35	6.38762674116059E-4	0.0	0.0	21.844618850312354	0.0
36	6.38762674116059E-4	0.0	0.0	22.22894105923885	0.0
37	6.38762674116059E-4	0.0	0.0	22.644775560088405	0.0
38	6.38762674116059E-4	0.0	0.0	23.033782028625083	0.0
39	6.38762674116059E-4	0.0	0.0	23.45302326373659	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.1627493E-6	45.000004	1
TCGTTTA	20	7.02902E-4	45.000004	38
TAAGTCG	20	7.02902E-4	45.000004	2
GCGATAT	20	7.02902E-4	45.000004	9
TCGACGG	40	6.7993824E-9	45.000004	2
TAATGCG	40	6.7993824E-9	45.000004	1
CGCACGG	25	3.8872706E-5	45.0	2
TCGACGT	25	3.8872706E-5	45.0	26
CGGTAGT	60	3.6379788E-12	41.250004	12
GTACGAG	60	3.6379788E-12	41.250004	1
TACACGG	50	1.0786607E-9	40.5	2
TACGAGG	90	0.0	40.0	2
TCGTTGA	80	0.0	39.375004	24
TTAATCG	80	0.0	39.375004	20
CGTTGAT	80	0.0	39.375004	25
CGATGGT	80	0.0	39.375004	10
TAATCGT	80	0.0	39.375004	21
ACGGGCT	155	0.0	39.193546	5
AACGGGA	115	0.0	39.130436	4
ATGGGTA	70	0.0	38.571426	5
>>END_MODULE