##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551965_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313208
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.257289085847106	33.0	31.0	34.0	30.0	34.0
2	32.356893821358334	34.0	31.0	34.0	30.0	34.0
3	32.3613668871804	34.0	31.0	34.0	30.0	34.0
4	35.92772534545733	37.0	35.0	37.0	35.0	37.0
5	35.99321217848841	37.0	35.0	37.0	35.0	37.0
6	35.97626178130827	37.0	35.0	37.0	35.0	37.0
7	36.148003882404026	37.0	35.0	37.0	35.0	37.0
8	35.977813465811856	37.0	35.0	37.0	35.0	37.0
9	37.888393655334475	39.0	38.0	39.0	35.0	39.0
10	37.615038568618935	39.0	37.0	39.0	35.0	39.0
11	37.556342749865905	39.0	37.0	39.0	35.0	39.0
12	37.375402288575	39.0	37.0	39.0	35.0	39.0
13	37.2997784220071	39.0	37.0	39.0	34.0	39.0
14	38.51576588082041	40.0	38.0	41.0	35.0	41.0
15	38.55953551633419	40.0	38.0	41.0	35.0	41.0
16	38.57800567035325	40.0	38.0	41.0	35.0	41.0
17	38.48030063089065	40.0	38.0	41.0	34.0	41.0
18	38.39049130290414	40.0	37.0	41.0	34.0	41.0
19	38.30279558631963	40.0	37.0	41.0	34.0	41.0
20	38.19357423820592	40.0	36.0	41.0	34.0	41.0
21	38.1183750095783	40.0	36.0	41.0	34.0	41.0
22	38.12800120048019	40.0	36.0	41.0	34.0	41.0
23	38.08210518249853	40.0	36.0	41.0	34.0	41.0
24	38.02528032489592	40.0	36.0	41.0	34.0	41.0
25	37.88274565145207	40.0	35.0	41.0	34.0	41.0
26	37.90701706214401	40.0	35.0	41.0	34.0	41.0
27	37.90845061428827	40.0	35.0	41.0	34.0	41.0
28	37.82384549564507	40.0	35.0	41.0	34.0	41.0
29	37.7906056039437	40.0	35.0	41.0	34.0	41.0
30	37.60103509488902	40.0	35.0	41.0	33.0	41.0
31	37.5406566882072	40.0	35.0	41.0	33.0	41.0
32	37.38294679573957	40.0	35.0	41.0	33.0	41.0
33	37.25423041557048	40.0	35.0	41.0	33.0	41.0
34	37.041640060279434	40.0	35.0	41.0	32.0	41.0
35	36.92220824500013	40.0	35.0	41.0	32.0	41.0
36	36.86474164133739	39.0	35.0	41.0	32.0	41.0
37	36.83425710709816	39.0	35.0	41.0	32.0	41.0
38	36.70687211054635	39.0	35.0	41.0	31.0	41.0
39	36.669242164951086	39.0	35.0	41.0	31.0	41.0
40	36.575451457178616	39.0	35.0	41.0	31.0	41.0
41	36.477736839416615	39.0	35.0	41.0	31.0	41.0
42	36.36313567980384	39.0	35.0	41.0	31.0	41.0
43	36.26695997547955	39.0	35.0	41.0	31.0	41.0
44	36.299424024929124	39.0	35.0	41.0	31.0	41.0
45	36.25021391535338	39.0	35.0	41.0	31.0	41.0
46	36.17857142857143	38.0	35.0	41.0	31.0	41.0
47	36.08237337488187	38.0	35.0	41.0	31.0	41.0
48	36.048427881791014	38.0	35.0	41.0	31.0	41.0
49	36.01978876657046	38.0	35.0	40.0	31.0	41.0
50	35.912811933283955	38.0	35.0	40.0	30.0	41.0
51	35.05676419503972	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	6.0
11	7.0
12	5.0
13	4.0
14	6.0
15	9.0
16	24.0
17	33.0
18	76.0
19	142.0
20	258.0
21	486.0
22	705.0
23	1040.0
24	1401.0
25	2062.0
26	2700.0
27	3356.0
28	3464.0
29	3746.0
30	4271.0
31	5182.0
32	6490.0
33	9092.0
34	19978.0
35	27666.0
36	22042.0
37	31556.0
38	58249.0
39	109051.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02856887435826	19.223008352277084	22.39885315828459	14.349569615080075
2	34.36661898802074	25.841293964394268	23.137978595693596	16.654108451891396
3	30.956105846593957	25.037036091032157	27.70491175193482	16.30194631043907
4	28.324308446782968	23.58752011442875	29.31885520165513	18.76931623713315
5	25.85917345661669	27.420755536257058	25.90163725064494	20.818433756481316
6	24.74266302265587	33.76127046563306	26.69950959107047	14.796556920640597
7	73.92691119000791	6.434062986896887	14.196316824602182	5.442708998493014
8	73.57123700544047	7.720428596970703	12.625475722203774	6.082858675385048
9	68.19685320936885	7.422543485479298	14.574021097800822	9.806582207351026
10	40.74544711501622	22.309774973819316	21.46784245613139	15.476935455033077
11	32.86282598145641	21.62141452325611	25.64174606012618	19.8740134351613
12	28.675832034941635	19.76162805547751	31.011340706495368	20.551199203085492
13	25.837781921279152	20.852596357692015	33.05311486296647	20.256506858062373
14	21.001059998467472	25.84416745421573	30.916515542387167	22.238257004929633
15	20.55630762943475	22.333720722331485	36.613688028402855	20.49628361983091
16	23.259622998135423	22.17216673903604	31.985134479323644	22.583075783504892
17	22.411943500804576	22.170570355801893	30.932479374728615	24.485006768664913
18	23.819634236673394	22.234106408520855	31.205460907767364	22.74079844703839
19	23.420538428137213	25.11781308267988	28.78438609486348	22.67726239431943
20	25.47093305407269	24.859198998748436	29.759456974279075	19.910410972899797
21	24.478301959081506	26.601491660493988	29.178054200403565	19.742152180020945
22	22.121721028837065	23.498122653316646	29.656330617353326	24.723825700492963
23	23.05815943398636	25.080138438354066	29.838318306045824	22.023383821613752
24	24.93550611734055	22.70503946259355	29.055771244668083	23.30368317539782
25	21.84331179280223	24.961686802380527	28.240338688666956	24.95466271615029
26	20.517036601874793	23.570279175499987	31.312737861101887	24.599946361523333
27	23.128400296288728	24.285139587749992	29.441776710684277	23.144683405277007
28	20.291627289213558	25.45177645526296	30.80796148246533	23.44863477305816
29	23.97863400679421	23.525580444943937	29.904408571939413	22.591376976322444
30	26.459094276008276	23.98118821996884	28.70456693315624	20.855150570866645
31	26.396835329876634	25.211680416847592	25.56895098464918	22.8225332686266
32	28.22948328267477	25.548517279252124	25.51148118821997	20.710518249853134
33	28.076549768843705	24.094212152946284	25.328535669586984	22.500702408623024
34	25.98017930576486	23.96267017445276	28.643265816965087	21.4138847028173
35	25.210403310260276	25.67973998109882	27.500255421317465	21.60960128732344
36	25.121644402441827	28.61133815228219	26.193456105846597	20.07356133942939
37	26.692804781487062	27.230466654747005	26.595744680851062	19.480983882914867
38	25.38760184925034	29.03693392250517	23.690646471354498	21.88481775688999
39	27.39936400091952	25.080457715000893	25.55075221577993	21.96942606829966
40	25.875775842251798	24.21585655538811	27.38403872187173	22.524328880488365
41	22.388636305586065	27.5194120201272	25.711986922428547	24.37996475185819
42	22.75803938596715	26.43035937779367	27.220569078695306	23.591032157543868
43	24.066115808025337	24.932632627519094	26.54274475747746	24.45850680697811
44	24.199254169753008	23.27079768077444	27.65446604173584	24.875482107736712
45	25.00063855329366	22.829557354856835	27.10499093254323	25.064813159306276
46	25.803300043421622	25.604390692447193	27.008250108554062	21.584059155577123
47	22.495913258920588	25.461354754667827	29.7939388521366	22.248793134274987
48	22.754208066205205	24.379645475211362	28.904753390717993	23.961393067865444
49	24.22926617455493	22.64182268652142	30.45196802125105	22.6769431176726
50	22.581798676917575	22.673111797910654	29.393565937013104	25.35152358815867
51	21.844588899389546	23.365303568235802	27.63977931598171	27.15032821639294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	303.0
1	527.5
2	752.0
3	2613.5
4	4475.0
5	2867.0
6	1259.0
7	1211.0
8	1163.0
9	1209.5
10	1256.0
11	1229.5
12	1203.0
13	1212.0
14	1221.0
15	1133.0
16	1045.0
17	994.5
18	944.0
19	960.0
20	976.0
21	1055.0
22	1134.0
23	1243.0
24	1352.0
25	1543.0
26	1906.5
27	2079.0
28	2463.0
29	2847.0
30	3122.0
31	3397.0
32	3966.5
33	4536.0
34	5245.5
35	5955.0
36	6512.0
37	7069.0
38	7252.5
39	7436.0
40	8930.0
41	10424.0
42	11946.5
43	13469.0
44	15242.0
45	17015.0
46	23037.0
47	29059.0
48	28698.5
49	28338.0
50	28846.0
51	29354.0
52	26024.5
53	22695.0
54	20889.0
55	19083.0
56	17870.5
57	16658.0
58	16131.5
59	15605.0
60	15315.0
61	15025.0
62	14238.0
63	13451.0
64	11807.5
65	10164.0
66	8420.0
67	6676.0
68	5799.5
69	4923.0
70	4039.5
71	3156.0
72	2692.5
73	2229.0
74	1911.0
75	1201.0
76	809.0
77	726.0
78	643.0
79	533.0
80	423.0
81	282.0
82	141.0
83	133.5
84	126.0
85	66.0
86	6.0
87	6.0
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	313208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.834573967500425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1586063583345	23.3646252389698
2	9.384634599832804	6.162809578523479
3	3.5070817726459693	3.4546060762204975
4	2.1431238384480933	2.8147423278014942
5	1.6164397647646938	2.653755551008766
6	1.3067269853285541	2.5743494313059556
7	1.1368914202840903	2.61305418026342
8	0.9960286859614441	2.616334205036263
9	0.8861358440844492	2.618630362605985
>10	7.744447639915533	43.37190590416756
>50	0.07792400875989908	1.7765880032327237
>100	0.033966875613289337	2.1147826545128083
>500	0.002997077259996118	0.7757811350896218
>1k	0.004995128766660197	3.0880353512616066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2666	0.8511915404459657	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	2055	0.6561135092334807	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	1637	0.5226558708589819	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	1634	0.521698040918495	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1422	0.4540113917907589	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	876	0.27968634262215525	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC	813	0.25957191387193174	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	676	0.21583101325636636	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT	435	0.13888534137059078	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	375	0.11972874256085413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22157799289928737	0.0
2	0.0	0.0	0.0	1.141414012413476	0.0
3	0.0	0.0	0.0	1.5462568005925774	0.0
4	0.0	0.0	0.0	2.1091415289520064	0.0
5	0.0	0.0	0.0	4.0088375775842255	0.0
6	0.0	0.0	0.0	4.801601491660494	0.0
7	0.0	0.0	0.0	5.7147327015912746	0.0
8	0.0	0.0	0.0	6.9535260912875785	0.0
9	0.0	0.0	0.0	7.477459068733877	0.0
10	0.0	0.0	0.0	9.15238435799852	0.0
11	0.0	0.0	0.0	10.695767668769635	0.0
12	0.0	0.0	0.0	12.300771372378739	0.0
13	0.0	0.0	0.0	12.755102040816327	0.0
14	0.0	0.0	0.0	12.95943909478685	0.0
15	0.0	0.0	0.0	13.374818012311307	0.0
16	0.0	0.0	0.0	14.069563995811091	0.0
17	0.0	0.0	0.0	14.818906285918622	0.0
18	0.0	0.0	0.0	15.68797731858701	0.0
19	0.0	0.0	0.0	16.302584863732726	0.0
20	0.0	0.0	0.0	16.79682511302393	0.0
21	0.0	0.0	0.0	17.327782176700467	0.0
22	3.1927664682894437E-4	0.0	0.0	17.873106689484306	0.0
23	3.1927664682894437E-4	0.0	0.0	18.4190697555618	0.0
24	3.1927664682894437E-4	0.0	0.0	18.858713698245257	0.0
25	3.1927664682894437E-4	0.0	0.0	19.278243212178488	0.0
26	3.1927664682894437E-4	0.0	0.0	19.67191131771858	0.0
27	3.1927664682894437E-4	0.0	0.0	20.078669765778653	0.0
28	3.1927664682894437E-4	0.0	0.0	20.4608439120329	0.0
29	3.1927664682894437E-4	0.0	0.0	20.829608439120328	0.0
30	3.1927664682894437E-4	0.0	0.0	21.23987893029552	0.0
31	3.1927664682894437E-4	0.0	0.0	21.617263926847336	0.0
32	3.1927664682894437E-4	0.0	0.0	22.0029501162167	0.0
33	3.1927664682894437E-4	0.0	0.0	22.41449771397921	0.0
34	3.1927664682894437E-4	0.0	0.0	22.82572603509489	0.0
35	3.1927664682894437E-4	0.0	0.0	23.213327884345226	0.0
36	3.1927664682894437E-4	0.0	0.0	23.590074327603382	0.0
37	3.1927664682894437E-4	0.0	0.0	23.958838854690814	0.0
38	3.1927664682894437E-4	0.0	0.0	24.37134428239381	0.0
39	3.1927664682894437E-4	0.0	0.0	24.809072565196292	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTG	20	7.0254196E-4	45.0	1
GTTTGCG	20	7.0254196E-4	45.0	1
CTGTACG	20	7.0254196E-4	45.0	1
TTCACGG	20	7.0254196E-4	45.0	2
ATGTACG	20	7.0254196E-4	45.0	1
CACTACC	20	7.0254196E-4	45.0	23
CATACCG	20	7.0254196E-4	45.0	5
TAAGCAG	25	3.884286E-5	45.0	1
TACCGAA	20	7.0254196E-4	45.0	7
GCGCGAC	80	0.0	42.1875	9
ATGGGCG	75	0.0	42.0	5
CGTTTTT	1315	0.0	41.74905	1
AAGGGCG	115	0.0	41.08696	5
CGACAGG	50	1.0768417E-9	40.5	2
CTCGTAC	45	1.920671E-8	40.0	29
TACGGTT	45	1.920671E-8	40.0	33
CGTTCAT	45	1.920671E-8	40.0	17
TCTCGTA	45	1.920671E-8	40.0	28
TCGTACG	45	1.920671E-8	40.0	30
ACGGTTA	45	1.920671E-8	40.0	34
>>END_MODULE