##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225980
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.302340915125235	33.0	31.0	34.0	30.0	34.0
2	32.38292769271617	34.0	31.0	34.0	30.0	34.0
3	32.373772015222585	34.0	31.0	34.0	30.0	34.0
4	35.94759270731923	37.0	35.0	37.0	35.0	37.0
5	36.015160633684395	37.0	35.0	37.0	35.0	37.0
6	36.00159748650323	37.0	35.0	37.0	35.0	37.0
7	36.159996459863706	37.0	36.0	37.0	35.0	37.0
8	35.975887246659	37.0	35.0	37.0	35.0	37.0
9	37.89128241437295	39.0	38.0	39.0	35.0	39.0
10	37.614930524825205	39.0	37.0	39.0	35.0	39.0
11	37.58786175767767	39.0	37.0	39.0	35.0	39.0
12	37.51222232055934	39.0	37.0	39.0	35.0	39.0
13	37.49497300646075	39.0	37.0	39.0	35.0	39.0
14	38.7466678467121	40.0	38.0	41.0	35.0	41.0
15	38.79322506416497	40.0	38.0	41.0	35.0	41.0
16	38.816868749446854	40.0	38.0	41.0	35.0	41.0
17	38.74651739091955	40.0	38.0	41.0	35.0	41.0
18	38.64369855739446	40.0	38.0	41.0	35.0	41.0
19	38.54950438091866	40.0	37.0	41.0	35.0	41.0
20	38.42605982830339	40.0	37.0	41.0	34.0	41.0
21	38.359385786352775	40.0	37.0	41.0	34.0	41.0
22	38.386574033100274	40.0	37.0	41.0	34.0	41.0
23	38.346247455527035	40.0	37.0	41.0	34.0	41.0
24	38.25982830338968	40.0	37.0	41.0	34.0	41.0
25	38.11160279670767	40.0	36.0	41.0	34.0	41.0
26	38.123736613859634	40.0	37.0	41.0	34.0	41.0
27	38.108593680856714	40.0	36.0	41.0	34.0	41.0
28	38.030723957872375	40.0	36.0	41.0	34.0	41.0
29	37.964138419329146	40.0	36.0	41.0	34.0	41.0
30	37.805743871139036	40.0	36.0	41.0	33.0	41.0
31	37.74733162226746	40.0	36.0	41.0	33.0	41.0
32	37.6039472519692	40.0	36.0	41.0	33.0	41.0
33	37.449066289052126	40.0	36.0	41.0	33.0	41.0
34	37.31212054164085	40.0	35.0	41.0	33.0	41.0
35	37.193348968935304	40.0	35.0	41.0	33.0	41.0
36	37.0970528365342	40.0	35.0	41.0	32.0	41.0
37	37.067762633861406	40.0	35.0	41.0	32.0	41.0
38	36.94577396229755	40.0	35.0	41.0	32.0	41.0
39	36.90304009204355	40.0	35.0	41.0	32.0	41.0
40	36.80964244623418	39.0	35.0	41.0	31.0	41.0
41	36.71169572528542	39.0	35.0	41.0	31.0	41.0
42	36.59532259491991	39.0	35.0	41.0	31.0	41.0
43	36.47561731126648	39.0	35.0	41.0	31.0	41.0
44	36.520997433401185	39.0	35.0	41.0	31.0	41.0
45	36.479737144880076	39.0	35.0	41.0	31.0	41.0
46	36.38035666873174	39.0	35.0	41.0	31.0	41.0
47	36.28389680502699	39.0	35.0	41.0	31.0	41.0
48	36.26377555535888	39.0	35.0	41.0	31.0	41.0
49	36.23709177803345	39.0	35.0	41.0	31.0	41.0
50	36.130515974865034	38.0	35.0	41.0	31.0	41.0
51	35.25482343570228	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	3.0
12	5.0
13	7.0
14	8.0
15	8.0
16	15.0
17	28.0
18	53.0
19	90.0
20	182.0
21	287.0
22	457.0
23	680.0
24	968.0
25	1416.0
26	1980.0
27	2360.0
28	2467.0
29	2587.0
30	2791.0
31	3429.0
32	4439.0
33	6146.0
34	11655.0
35	16652.0
36	16121.0
37	23568.0
38	43131.0
39	84326.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.97999822993185	19.26984688910523	22.24798654748208	14.502168333480839
2	34.38180369944243	26.289052128506945	22.37366138596336	16.955482786087263
3	31.62403752544473	25.685458890167272	26.59748650323037	16.093017081157623
4	28.58350296486415	24.306575803168425	28.447207717497125	18.66271351447031
5	26.548367112133818	27.49092840074343	25.273033011770952	20.687671475351802
6	24.906628905212852	34.445083635719975	25.893441897513053	14.75484556155412
7	73.8786618284804	6.717851137268785	14.186653686166917	5.2168333480839015
8	73.34365873086114	8.418886627135144	12.66882024957961	5.568634392424109
9	66.97672360385874	8.288344101247898	14.920346933356935	9.81458536153642
10	37.29312328524648	24.711036374900434	21.359412337374987	16.636428002478095
11	29.74643773785291	23.888397203292328	26.14656164262324	20.218603416231527
12	26.3377290025666	21.359854854411893	31.04566775820869	21.256748384812816
13	24.73935746526241	22.45419948668024	32.734755288078595	20.071687759978758
14	20.584565005752722	27.47455527037791	30.315957164350827	21.62492255951854
15	19.336666961678027	24.323391450570846	35.13187007699796	21.208071510753165
16	21.31162049738915	24.489335339410566	31.359412337374987	22.839631825825293
17	21.361182405522612	23.756527126294362	30.514647313921582	24.36764315426144
18	22.58828214886273	24.003451632887867	29.941587751128417	23.466678467120985
19	22.959553942826798	25.008407823701212	29.220284980971766	22.81175325250022
20	25.26816532436499	24.951765642977257	29.53358704310116	20.246481989556596
21	23.76227984777414	26.281971855916453	29.634923444552612	20.32082485175679
22	22.08912293123285	24.252146207628993	28.766262501106294	24.89246836003186
23	22.6683777325427	26.030179661916986	29.00964687140455	22.291795734135764
24	22.895831489512346	24.948225506682007	28.81493937516594	23.341003628639704
25	21.63908310469953	27.102398442340032	27.667492698468894	23.59102575449155
26	20.851845296043898	27.09797327197097	28.874236658111336	23.175944773873795
27	23.273298521993098	26.04478272413488	28.334365873086114	22.34755288078591
28	20.30400920435437	26.683777325427027	30.129657491813433	22.88255597840517
29	22.69935392512612	26.340384104788033	28.42065669528277	22.53960527480308
30	22.76219134436676	26.897955571289494	28.317992742720595	22.021860341623153
31	23.22285157978582	26.55765996990884	26.770068147623682	23.449420302681652
32	25.42658642357731	27.467032480750508	25.733250730153113	21.37313036551907
33	24.430923090538986	26.48287459067174	25.473935746526244	23.61226657226303
34	22.957783874679176	26.298787503318877	27.079830073457828	23.66359854854412
35	24.568545889016725	26.54615452694929	25.358881316930702	23.52641826710328
36	23.02239136206744	29.89777856447473	25.759801752367466	21.320028321090362
37	23.632179838923797	27.600230108859193	27.31215151783344	21.45543853438357
38	24.49995574829631	26.512080715107533	26.248783078148506	22.73918045844765
39	25.640764669439776	24.661916983803877	26.156297017435172	23.54102132932118
40	25.131870076997963	24.06142136472254	28.267103283476413	22.53960527480308
41	21.78998141428445	27.060359323833964	26.714311000973538	24.435348260908047
42	22.61881582440924	27.398442340030094	27.8104257013895	22.172316134171165
43	23.754314541109835	26.20497389149482	27.428976015576602	22.611735551818747
44	23.839277812195768	24.765023453402957	28.054695105761574	23.341003628639704
45	24.01584210992123	23.6848393663156	26.956367820161077	25.342950703602092
46	24.405699619435346	26.002743605628815	26.5996990884149	22.99185768652093
47	21.83025046464289	26.135056199663687	29.866359854854412	22.16833348083901
48	22.68519337994513	25.273033011770952	28.18612266572263	23.855650942561287
49	22.75997875918223	24.2972829453934	29.976546597043985	22.966191698380385
50	21.930259314983626	23.81405434109213	29.102575449154795	25.153110894769448
51	21.568280378794583	23.387467917514822	27.448004248163553	27.59624745552704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	209.0
1	386.0
2	563.0
3	1866.0
4	3169.0
5	2068.5
6	968.0
7	1019.0
8	1070.0
9	1022.0
10	974.0
11	939.5
12	905.0
13	895.5
14	886.0
15	861.5
16	837.0
17	845.0
18	853.0
19	855.5
20	858.0
21	976.5
22	1095.0
23	1113.0
24	1131.0
25	1330.0
26	1782.5
27	2036.0
28	2282.5
29	2529.0
30	2917.0
31	3305.0
32	3651.5
33	3998.0
34	4549.5
35	5101.0
36	5142.0
37	5183.0
38	5777.0
39	6371.0
40	7349.0
41	8327.0
42	9464.0
43	10601.0
44	11523.5
45	12446.0
46	16445.0
47	20444.0
48	20782.0
49	21120.0
50	20764.5
51	20409.0
52	18113.0
53	15817.0
54	14268.0
55	12719.0
56	11825.5
57	10932.0
58	10634.0
59	10336.0
60	9911.5
61	9487.0
62	8725.5
63	7964.0
64	7086.0
65	6208.0
66	5291.5
67	4375.0
68	3783.0
69	3191.0
70	2881.5
71	2572.0
72	2300.5
73	2029.0
74	1776.5
75	1204.5
76	885.0
77	662.0
78	439.0
79	384.5
80	330.0
81	249.5
82	169.0
83	117.0
84	65.0
85	39.0
86	13.0
87	9.0
88	5.0
89	2.5
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	225980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.72169620859818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.3378992313615	31.00908465167343
2	9.857985673407969	8.817316810297287
3	5.289134276892611	7.09617169013025
4	4.093997339317979	7.323620211511517
5	3.135995517865849	7.012351943076103
6	2.32499662275959	6.238667558944268
7	1.7599974422369153	5.509704965774042
8	1.2489981848340923	4.468585390979267
9	0.9239986571545079	3.719050851817492
>10	1.9529971617129	11.84944340665637
>50	0.03599994768134377	1.1050715073161188
>100	0.0319999534945278	2.6479677842245
>500	0.0019999970934079874	0.6542774646715831
>1k	0.003999994186815975	2.5486857629277866
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2030	0.8983095849190194	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	1356	0.6000531020444286	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	1184	0.5239401716966103	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	1129	0.49960173466678465	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	821	0.36330648729976106	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	642	0.2840959376936012	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	448	0.19824763253385255	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	414	0.18320205327905123	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	330	0.1460306221789539	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	259	0.11461191255863351	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGAT	240	0.10620408885742101	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	239	0.1057615718205151	No Hit
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	233	0.10310646959907957	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	230	0.1017789184883618	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	227	0.10045136737764403	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2057704221612532	0.0
2	0.0	0.0	0.0	0.9620320382334719	0.0
3	0.0	0.0	0.0	1.3527745818214	0.0
4	0.0	0.0	0.0	1.9276042127621913	0.0
5	0.0	0.0	0.0	3.668023718913178	0.0
6	0.0	0.0	0.0	4.540224798654748	0.0
7	0.0	0.0	0.0	5.485441189485795	0.0
8	0.0	0.0	0.0	6.649703513585273	0.0
9	0.0	0.0	0.0	7.15771307195327	0.0
10	0.0	0.0	0.0	8.752986989999116	0.0
11	0.0	0.0	0.0	10.353128595450924	0.0
12	0.0	0.0	0.0	11.991326666076644	0.0
13	0.0	0.0	0.0	12.515266837773254	0.0
14	0.0	0.0	0.0	12.752455969554829	0.0
15	0.0	0.0	0.0	13.238782193114435	0.0
16	0.0	0.0	0.0	13.956987344012745	0.0
17	0.0	0.0	0.0	14.771661208956544	4.425170369059209E-4
18	0.0	0.0	0.0	15.6801486857244	4.425170369059209E-4
19	4.425170369059209E-4	0.0	0.0	16.308080361093904	4.425170369059209E-4
20	4.425170369059209E-4	0.0	0.0	16.810337197982122	4.425170369059209E-4
21	4.425170369059209E-4	0.0	0.0	17.407292680768208	4.425170369059209E-4
22	4.425170369059209E-4	0.0	0.0	18.03212673687937	4.425170369059209E-4
23	4.425170369059209E-4	0.0	0.0	18.637490043366668	4.425170369059209E-4
24	4.425170369059209E-4	0.0	0.0	19.119391096557216	4.425170369059209E-4
25	4.425170369059209E-4	0.0	0.0	19.568545889016725	4.425170369059209E-4
26	4.425170369059209E-4	0.0	0.0	19.987609522966633	4.425170369059209E-4
27	4.425170369059209E-4	0.0	0.0	20.41331091247013	4.425170369059209E-4
28	4.425170369059209E-4	0.0	0.0	20.841224887158155	4.425170369059209E-4
29	4.425170369059209E-4	0.0	0.0	21.25055314629613	4.425170369059209E-4
30	4.425170369059209E-4	0.0	0.0	21.73466678467121	4.425170369059209E-4
31	4.425170369059209E-4	0.0	0.0	22.203734843791487	4.425170369059209E-4
32	4.425170369059209E-4	0.0	0.0	22.649349499955747	4.425170369059209E-4
33	4.425170369059209E-4	0.0	0.0	23.083458713160457	4.425170369059209E-4
34	4.425170369059209E-4	0.0	0.0	23.500309761925834	4.425170369059209E-4
35	4.425170369059209E-4	0.0	0.0	23.924683600318613	4.425170369059209E-4
36	4.425170369059209E-4	0.0	0.0	24.383573767590054	4.425170369059209E-4
37	4.425170369059209E-4	0.0	0.0	24.826975838569783	4.425170369059209E-4
38	4.425170369059209E-4	0.0	0.0	25.255332330294717	4.425170369059209E-4
39	4.425170369059209E-4	0.0	0.0	25.66820072572794	4.425170369059209E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	30	2.157738E-6	45.000004	31
CCATTAC	30	2.157738E-6	45.000004	6
ACACGAC	40	6.7739165E-9	45.0	26
AATTTGC	25	3.8808284E-5	45.0	45
CTCAACC	20	7.021246E-4	45.0	36
GATTTGC	20	7.021246E-4	45.0	37
TTATCTT	25	3.8808284E-5	45.0	26
ATCGTTG	20	7.021246E-4	45.0	23
TGAATAG	20	7.021246E-4	45.0	1
TGCGTAG	20	7.021246E-4	45.0	1
TAGGGCA	55	6.002665E-11	40.909092	5
TTATGGG	105	0.0	40.714287	3
CGTTTTT	995	0.0	40.703514	1
TGATAGG	45	1.9164872E-8	40.0	2
GAATGAC	45	1.9164872E-8	40.0	1
GACACGA	45	1.9164872E-8	40.0	25
CGACCAA	45	1.9164872E-8	40.0	29
CACGACC	45	1.9164872E-8	40.0	27
AATGACT	45	1.9164872E-8	40.0	2
AGACACG	45	1.9164872E-8	40.0	24
>>END_MODULE