##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453564
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27112160577118	33.0	31.0	34.0	30.0	34.0
2	32.36543023696766	34.0	31.0	34.0	30.0	34.0
3	32.36821705426357	34.0	31.0	34.0	30.0	34.0
4	35.92538208499793	37.0	35.0	37.0	35.0	37.0
5	35.961771657362576	37.0	35.0	37.0	35.0	37.0
6	35.95184803026695	37.0	35.0	37.0	35.0	37.0
7	36.09343775079151	37.0	35.0	37.0	35.0	37.0
8	35.897586669136	37.0	35.0	37.0	35.0	37.0
9	37.768502350274716	39.0	38.0	39.0	35.0	39.0
10	37.54200950692736	39.0	37.0	39.0	35.0	39.0
11	37.46689772556905	39.0	37.0	39.0	35.0	39.0
12	37.25511063488284	39.0	37.0	39.0	35.0	39.0
13	37.16620146219718	39.0	37.0	39.0	34.0	39.0
14	38.40495056926917	40.0	37.0	41.0	34.0	41.0
15	38.3862321524636	40.0	37.0	41.0	34.0	41.0
16	38.45503831873782	40.0	37.0	41.0	34.0	41.0
17	38.30884285348925	40.0	37.0	41.0	34.0	41.0
18	38.2360813468441	40.0	37.0	41.0	34.0	41.0
19	38.14108262560521	40.0	37.0	41.0	34.0	41.0
20	37.886529795133654	40.0	35.0	41.0	33.0	41.0
21	37.90008686756445	40.0	35.0	41.0	34.0	41.0
22	37.89950701554797	40.0	35.0	41.0	34.0	41.0
23	37.78172429910663	40.0	35.0	41.0	34.0	41.0
24	37.78079829968869	40.0	35.0	41.0	33.0	41.0
25	37.68946609519274	40.0	35.0	41.0	33.0	41.0
26	37.58410720427547	40.0	35.0	41.0	33.0	41.0
27	37.53565979663289	40.0	35.0	41.0	33.0	41.0
28	37.470010847421754	40.0	35.0	41.0	33.0	41.0
29	37.35392138705894	40.0	35.0	41.0	33.0	41.0
30	37.20907082572691	39.0	35.0	41.0	33.0	41.0
31	37.13015362771296	39.0	35.0	41.0	32.0	41.0
32	36.86929959167835	39.0	35.0	41.0	32.0	41.0
33	36.68185526188145	39.0	35.0	41.0	31.0	41.0
34	36.4465610145426	39.0	35.0	41.0	30.0	41.0
35	36.35103315077916	39.0	35.0	41.0	30.0	41.0
36	36.29887292642273	39.0	35.0	41.0	30.0	41.0
37	36.110352673492606	39.0	35.0	41.0	30.0	41.0
38	36.028212115600006	39.0	35.0	41.0	30.0	41.0
39	36.006735543385275	39.0	35.0	41.0	29.0	41.0
40	35.8899978834299	39.0	35.0	41.0	29.0	41.0
41	35.85786570362727	38.0	35.0	41.0	29.0	41.0
42	35.656006649557725	38.0	35.0	41.0	28.0	41.0
43	35.48023873146899	38.0	35.0	41.0	27.0	41.0
44	35.53672249120301	38.0	35.0	41.0	28.0	41.0
45	35.49562134560944	38.0	35.0	41.0	28.0	41.0
46	35.44258803608752	38.0	35.0	40.0	28.0	41.0
47	35.271566967396	37.0	35.0	40.0	27.0	41.0
48	35.24867493892813	37.0	35.0	40.0	27.0	41.0
49	35.236248908643546	37.0	35.0	40.0	27.0	41.0
50	35.0859173126615	37.0	35.0	40.0	27.0	41.0
51	34.27678343078375	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	9.0
11	5.0
12	8.0
13	16.0
14	10.0
15	25.0
16	41.0
17	82.0
18	144.0
19	288.0
20	703.0
21	1487.0
22	2180.0
23	2761.0
24	3349.0
25	4069.0
26	5043.0
27	5612.0
28	5838.0
29	6247.0
30	6763.0
31	8229.0
32	10301.0
33	13952.0
34	28196.0
35	43330.0
36	32579.0
37	46516.0
38	80645.0
39	144949.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.07678739935268	17.951380621036943	20.164078277817463	13.80775370179291
2	33.168417246518686	27.713398770625535	22.679048601740877	16.4391353811149
3	30.539019851663713	27.867291054845623	26.245248741081745	15.348440352408922
4	26.216807330387777	23.484888571403374	32.234260214655485	18.064043883553367
5	28.775431912585653	27.083719166424142	25.011244278646455	19.129604642343747
6	25.085765184185693	36.087079221454964	25.012126182854015	13.815029411505323
7	72.92223368697692	6.08469808009454	16.47904154650722	4.514026686421321
8	73.4868728558704	10.568740023458652	11.410958541683202	4.533428578987751
9	68.12070622888942	6.918538508347223	13.055930364843771	11.904824897919589
10	43.79734723214364	21.318931837623797	19.308190244375655	15.575530685856902
11	35.89328077184256	21.092061980227708	24.639962607261598	18.374694640668128
12	29.363662019031494	19.825647538164404	28.383645968374914	22.427044474429188
13	27.275533331569523	20.655519397483047	31.590911095236834	20.478036175710596
14	21.82448342461042	26.102821211560002	30.47993226975686	21.592763094072723
15	21.463123175560668	21.208693811678174	34.948981841592364	22.379201171168788
16	25.09921422335106	20.803017876198286	29.435537211948038	24.662230688502614
17	23.841618823363405	22.863587057173852	30.503523207309225	22.79127091215352
18	24.840816290534523	21.580857387270594	29.305676817384096	24.272649504810786
19	24.272208552707006	25.142427529521743	26.73536700443598	23.84999691333527
20	29.051908881657273	23.491943805063894	28.68768244393294	18.76846486934589
21	25.50753587145364	27.98282050603663	27.330872820594227	19.178770801915494
22	23.414777186901958	22.029085200765493	31.229550846187088	23.32658676614546
23	26.36915628224462	24.834863437133457	28.210131315536508	20.58584896508541
24	26.119356915451842	22.928627492481766	27.677681650219156	23.274333941847235
25	22.81243661313508	26.398479597146157	26.81738409573952	23.97169969397924
26	21.931855261881456	22.93105272905257	31.36470266599642	23.772389343069555
27	24.784374421250362	23.81053170004674	27.40803943875616	23.997054439946734
28	20.552777557301727	28.078066160453652	28.812692365355275	22.556463916889346
29	24.461817957333473	24.986550960834634	28.354763605577162	22.196867476254727
30	24.228554294432538	25.903731336702208	28.649981039059536	21.217733329805714
31	25.893148486211427	24.591457875845528	25.10979707384184	24.405596564101206
32	28.42906403506451	26.298383469587534	25.46432256528296	19.808229930064996
33	27.496009383460766	23.151969733047597	25.977811290137666	23.37420959335397
34	23.939951142506903	26.398259121094263	26.354163910716018	23.307625825682816
35	26.674956566217777	26.0600488574931	25.71456288417952	21.550431692109605
36	24.34761136245381	31.540863031457523	24.003227769399686	20.10829783668898
37	25.641144358899737	28.33800742563343	24.37583229709589	21.645015918370948
38	23.71484509352594	28.520120644495595	24.74116111507968	23.023873146898787
39	25.32498170048769	26.417881489712585	24.830674392147525	23.426462417652193
40	25.30447742766181	22.675080032806836	27.432953232619873	24.587489306911483
41	23.23927824959653	25.454180666895958	24.960093834607687	26.346447248899825
42	24.866391512553907	24.53898457549541	25.87727421047526	24.717349701475424
43	25.18784559621134	24.090756762000513	25.476669224188868	25.244728417599283
44	24.090536285948623	23.517078074979494	27.231879073295058	25.160506565776824
45	24.306382340750147	23.477833337742855	26.61697136457038	25.598812956936616
46	25.607191046908483	25.86823469234772	25.637396266017586	22.887177994726212
47	20.903775431912585	25.6470972123008	30.481475602120096	22.96765175366652
48	23.631946098014833	24.196364790856418	28.174414195130126	23.997274915998624
49	24.559709324373184	22.420650668924342	29.858630755527333	23.161009251175138
50	22.60276388778651	23.460415729643447	28.60235821185103	25.334462170719018
51	22.25573458210969	22.452619696448572	27.826723461297632	27.464922260144103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	335.0
1	752.5
2	1170.0
3	4351.5
4	7533.0
5	4847.5
6	2162.0
7	2174.0
8	2186.0
9	2045.5
10	1905.0
11	1818.5
12	1732.0
13	1737.0
14	1742.0
15	1721.0
16	1700.0
17	1721.5
18	1743.0
19	1653.5
20	1564.0
21	1656.0
22	1748.0
23	1772.5
24	1797.0
25	1982.0
26	2548.0
27	2929.0
28	3329.5
29	3730.0
30	4394.0
31	5058.0
32	5682.0
33	6306.0
34	7260.5
35	8215.0
36	8385.0
37	8555.0
38	9327.5
39	10100.0
40	11635.5
41	13171.0
42	15212.0
43	17253.0
44	19229.5
45	21206.0
46	31687.0
47	42168.0
48	37895.5
49	33623.0
50	34933.0
51	36243.0
52	34374.0
53	32505.0
54	30936.0
55	29367.0
56	28348.5
57	27330.0
58	26198.0
59	25066.0
60	24585.5
61	24105.0
62	22556.5
63	21008.0
64	18987.0
65	16966.0
66	14207.5
67	11449.0
68	9773.5
69	8098.0
70	7220.5
71	6343.0
72	5686.0
73	5029.0
74	4242.0
75	2752.5
76	2050.0
77	1645.5
78	1241.0
79	1019.5
80	798.0
81	600.0
82	402.0
83	300.5
84	199.0
85	130.0
86	61.0
87	43.5
88	26.0
89	18.5
90	11.0
91	8.0
92	5.0
93	5.5
94	6.0
95	4.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.042912203394756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01991709742647	23.797477975460314
2	8.039402375778955	5.312905337412545
3	3.3163329918432476	3.2874389966009367
4	2.178769514392608	2.8797155910203194
5	1.7403886258861345	2.8753754282471187
6	1.4656386882899999	2.9057382299439065
7	1.2611745956463618	2.917101700596623
8	1.045628855166614	2.764049796688527
9	0.9751234496031214	2.8998826679436633
>10	7.8496305854622275	41.746924897565464
>50	0.07110112654318251	1.5960325488839224
>100	0.028842909824121202	1.669173882189254
>500	0.004024592068482029	0.9331061006528694
>1k	0.003353826723735024	3.239052863406421
>5k	6.707653447470047E-4	1.1760239833881532
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	5306	1.1698459313349385	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	4418	0.9740631972555142	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3846	0.8479508955737227	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC	3659	0.8067218738700601	No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC	1552	0.34217883253521003	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAATCGGT	1139	0.2511222231041264	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT	940	0.20724748877776894	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTA	780	0.17197132047517	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT	744	0.16403418260708522	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC	618	0.13625420006878852	No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT	570	0.12567134957800885	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTC	558	0.1230256369553139	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	465	0.10252136412942825	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12986039456394247	0.0
2	2.2047605189124358E-4	0.0	0.0	0.6493019728197124	0.0
3	2.2047605189124358E-4	0.0	0.0	0.8124542512192325	0.0
4	2.2047605189124358E-4	0.0	0.0	1.190350204160824	0.0
5	2.2047605189124358E-4	0.0	0.0	2.61330264306691	0.0
6	2.2047605189124358E-4	0.0	0.0	3.095704244604951	0.0
7	2.2047605189124358E-4	0.0	0.0	3.7077457646550434	0.0
8	2.2047605189124358E-4	0.0	0.0	4.431568643013996	0.0
9	2.2047605189124358E-4	0.0	0.0	4.741998924076867	0.0
10	2.2047605189124358E-4	0.0	0.0	6.543508744080218	0.0
11	2.2047605189124358E-4	0.0	0.0	7.488028150382306	0.0
12	2.2047605189124358E-4	0.0	0.0	9.199804217265921	0.0
13	2.2047605189124358E-4	0.0	0.0	9.567117319716733	0.0
14	2.2047605189124358E-4	0.0	0.0	9.741954828866488	0.0
15	2.2047605189124358E-4	0.0	0.0	10.166812180860916	0.0
16	2.2047605189124358E-4	0.0	0.0	10.667292818654039	0.0
17	2.2047605189124358E-4	0.0	0.0	11.19070296584385	0.0
18	2.2047605189124358E-4	0.0	0.0	11.72271167905742	0.0
19	2.2047605189124358E-4	0.0	0.0	12.35195033115503	0.0
20	2.2047605189124358E-4	0.0	0.0	12.738885802224162	0.0
21	2.2047605189124358E-4	0.0	0.0	13.128907938019772	0.0
22	2.2047605189124358E-4	0.0	0.0	13.553103861858524	0.0
23	2.2047605189124358E-4	0.0	0.0	13.926810769814184	0.0
24	2.2047605189124358E-4	0.0	0.0	14.268548650245611	0.0
25	2.2047605189124358E-4	0.0	0.0	14.558254182430705	0.0
26	2.2047605189124358E-4	0.0	0.0	14.835392579657997	0.0
27	2.2047605189124358E-4	0.0	0.0	15.135901438385762	0.0
28	2.2047605189124358E-4	0.0	0.0	15.411276027197927	0.0
29	2.2047605189124358E-4	0.0	0.0	15.6762882415712	0.0
30	2.2047605189124358E-4	0.0	0.0	16.002151846266457	0.0
31	2.2047605189124358E-4	0.0	0.0	16.297810231852615	0.0
32	2.2047605189124358E-4	0.0	0.0	16.618602887354374	0.0
33	2.2047605189124358E-4	0.0	0.0	16.939616018908026	0.0
34	2.2047605189124358E-4	0.0	0.0	17.240786305791463	0.0
35	2.2047605189124358E-4	0.0	0.0	17.544381829245708	0.0
36	2.2047605189124358E-4	0.0	0.0	17.875316383134464	0.0
37	2.2047605189124358E-4	0.0	0.0	18.210660458061046	0.0
38	2.2047605189124358E-4	0.0	0.0	18.613029252762566	0.0
39	2.2047605189124358E-4	0.0	0.0	18.973287121552858	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTACG	20	7.028764E-4	45.000004	31
CGTTAGG	40	6.7993824E-9	45.000004	2
CGATGTA	20	7.028764E-4	45.000004	10
TTACGAG	20	7.028764E-4	45.000004	1
ATACGAT	20	7.028764E-4	45.000004	11
GATGAAT	820	0.0	42.80488	20
CGATGAA	785	0.0	42.707005	19
CCGATGA	775	0.0	41.806454	18
TATGCGG	70	0.0	41.785717	2
ATGAATG	850	0.0	41.02941	21
CGTTTTT	1860	0.0	40.887096	1
TACGGCT	490	0.0	40.867348	7
TGAATGA	845	0.0	40.739643	22
GTCGAAT	105	0.0	40.714287	43
TGCGTAG	45	1.9239451E-8	40.000004	1
TAAGCGG	75	0.0	39.0	2
CATACGA	35	6.2399267E-6	38.57143	18
ACATACG	35	6.2399267E-6	38.57143	17
AGGGAAC	240	0.0	38.437504	6
ACGGCTG	530	0.0	38.207546	8
>>END_MODULE