##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470452
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19840918946035	33.0	31.0	34.0	30.0	34.0
2	32.29397473068453	34.0	31.0	34.0	30.0	34.0
3	32.316761327404286	34.0	31.0	34.0	30.0	34.0
4	35.8943059015585	37.0	35.0	37.0	35.0	37.0
5	35.95844634521694	37.0	35.0	37.0	35.0	37.0
6	35.94633671447884	37.0	35.0	37.0	35.0	37.0
7	36.147553841837215	37.0	35.0	37.0	35.0	37.0
8	35.95488168824875	37.0	35.0	37.0	35.0	37.0
9	37.867722955795706	39.0	38.0	39.0	35.0	39.0
10	37.548344995876306	39.0	37.0	39.0	35.0	39.0
11	37.52409172455426	39.0	37.0	39.0	35.0	39.0
12	37.50830265361822	39.0	37.0	39.0	35.0	39.0
13	37.48898931240594	39.0	37.0	39.0	35.0	39.0
14	38.753328713662604	40.0	38.0	41.0	35.0	41.0
15	38.7836059789309	40.0	38.0	41.0	35.0	41.0
16	38.79789649103415	40.0	38.0	41.0	35.0	41.0
17	38.74891806177889	40.0	38.0	41.0	35.0	41.0
18	38.670999379320314	40.0	38.0	41.0	35.0	41.0
19	38.61950209585675	40.0	38.0	41.0	35.0	41.0
20	38.53582299575727	40.0	38.0	41.0	35.0	41.0
21	38.45632285546666	40.0	38.0	41.0	34.0	41.0
22	38.47037954987969	40.0	38.0	41.0	34.0	41.0
23	38.432307653065564	40.0	38.0	41.0	34.0	41.0
24	38.380995298138814	40.0	37.0	41.0	34.0	41.0
25	38.21680426483467	40.0	37.0	41.0	34.0	41.0
26	38.25333296489334	40.0	37.0	41.0	34.0	41.0
27	38.25048464030337	40.0	37.0	41.0	34.0	41.0
28	38.185094334809925	40.0	37.0	41.0	34.0	41.0
29	38.16172744509535	40.0	37.0	41.0	34.0	41.0
30	38.01876705806331	40.0	37.0	41.0	34.0	41.0
31	37.99149966415277	40.0	37.0	41.0	34.0	41.0
32	37.87610213156709	40.0	37.0	41.0	34.0	41.0
33	37.79366651645651	40.0	37.0	41.0	33.0	41.0
34	37.693492641119605	40.0	37.0	41.0	33.0	41.0
35	37.599978318723274	40.0	37.0	41.0	33.0	41.0
36	37.523996071862804	40.0	36.0	41.0	33.0	41.0
37	37.49457117835613	40.0	36.0	41.0	33.0	41.0
38	37.429737784088495	40.0	36.0	41.0	33.0	41.0
39	37.37413593735386	40.0	36.0	41.0	33.0	41.0
40	37.260128557217314	40.0	36.0	41.0	33.0	41.0
41	37.16505190752723	40.0	35.0	41.0	32.0	41.0
42	37.0667910860194	40.0	35.0	41.0	32.0	41.0
43	36.98902119663643	39.0	35.0	41.0	32.0	41.0
44	37.05575914227169	39.0	35.0	41.0	32.0	41.0
45	37.03128693256698	39.0	35.0	41.0	32.0	41.0
46	36.94572878848427	39.0	35.0	41.0	32.0	41.0
47	36.85759014734766	39.0	35.0	41.0	32.0	41.0
48	36.837936707676874	39.0	35.0	41.0	32.0	41.0
49	36.82474301310229	39.0	35.0	41.0	32.0	41.0
50	36.73697635465467	39.0	35.0	41.0	32.0	41.0
51	35.942553119127986	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	8.0
11	7.0
12	8.0
13	10.0
14	15.0
15	26.0
16	22.0
17	60.0
18	100.0
19	195.0
20	278.0
21	499.0
22	775.0
23	1213.0
24	1689.0
25	2349.0
26	3259.0
27	3862.0
28	4256.0
29	4772.0
30	5340.0
31	6888.0
32	8861.0
33	12065.0
34	21882.0
35	32698.0
36	31872.0
37	47557.0
38	93380.0
39	186280.0
40	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.092600307789105	20.83400644486579	22.333202962257573	13.740190285087534
2	32.60077542448539	26.551699216923296	23.307797607407345	17.539727751183968
3	30.756166410175744	25.22744084412437	26.682212000374108	17.33418074532577
4	28.16674177174292	24.910724154642768	27.697618460544327	19.224915613069985
5	25.12349825274417	28.32722573184937	25.457644988224093	21.09163102718237
6	25.06546895326197	34.90579272699447	24.625254011036198	15.403484308707371
7	77.02698681268228	5.809519355853519	12.24545755996361	4.9180362715006
8	77.08416586601821	7.031535629564758	10.173620263066157	5.7106782413508705
9	70.14339401256665	8.589186569511874	12.787276916667375	8.480142501254113
10	34.61011962963278	31.702277809425826	18.685221871731866	15.002380689209527
11	27.00360504366014	24.37761982093816	28.51895623783085	20.09981889757085
12	25.4491425267615	21.114800234667936	32.00092676829942	21.435130470271144
13	23.999260285852756	22.983003579536277	33.48843240118014	19.52930373343083
14	20.762160645506874	27.549037946485505	29.808354518633145	21.880446889374475
15	18.5574723882564	25.35646569681923	35.26374635456965	20.822315560354724
16	20.323433634037052	24.618664603402685	32.47451387176588	22.583387890794384
17	20.631222738982935	24.054738846896175	30.45050292059551	24.863535493525376
18	20.58764762398714	24.867574162720107	31.54498227236785	22.999795940924898
19	21.871519304838753	26.178653720252015	29.085007609703005	22.864819365206227
20	22.95409521056346	26.054092659825017	29.713977196398357	21.277834933213168
21	22.489860814705857	26.777652130291717	29.737996650030183	20.99449040497224
22	20.880982544446617	24.190990791834235	28.515555253245818	26.41247141047333
23	20.2488245347028	26.85098586040659	29.42829449125522	23.471895113635398
24	22.13785891015449	24.77893600197257	29.533087328781683	23.550117759091258
25	20.915204951833555	27.578796561604584	27.237422733881463	24.268575752680402
26	19.725072908607043	27.01848435121968	28.654145375086088	24.602297365087193
27	22.56021868330882	25.367518896720604	28.370375723772035	23.70188669619855
28	19.843469684473654	25.96247863756557	29.72481783476316	24.469233843197603
29	20.843146590938076	23.735046295902666	29.221046993104505	26.200760120054756
30	21.551614192308673	24.726008179367927	27.51800396214704	26.204373666176355
31	21.24361252582623	25.78286413916829	25.376871604329455	27.59665173067603
32	21.13605638832442	27.248475933782828	26.48623026366133	25.12923741423142
33	20.80637344511236	25.805183100507595	25.906574953449024	27.481868500931018
34	19.52398969501671	26.598037631894435	26.857362706503533	27.020609966585322
35	19.84006869988862	25.709317847516854	26.701129977128378	27.749483475466146
36	20.22119153494937	27.82302976711758	25.270590836047035	26.685187861886018
37	19.406018042223224	28.079591541751338	28.382066608283097	24.132323807742342
38	19.51846309506602	28.06386198804554	26.001589960293504	26.416084956594933
39	20.833368760256093	26.004353260268847	28.215843486689394	24.94643449278566
40	20.37784938739765	26.106595359356533	29.577725251460297	23.937830001785517
41	18.57957878805914	28.818880565923834	27.23210869546734	25.369431950549686
42	19.923392822222034	27.748208106246757	27.958218904372817	24.37018016715839
43	20.96770765136507	26.037937983046096	27.769251698366677	25.22510266722216
44	20.676923469344374	24.63566952632787	28.601642675554572	26.08576432877318
45	20.63844983122614	23.866621887036295	27.831107105507048	27.66382117623052
46	22.195888209636692	25.847695407820563	27.499298546929335	24.45711783561341
47	19.212587043949224	26.071522705823334	30.05258772414614	24.663302526081303
48	19.579043132987	25.317992058700995	28.22689668659077	26.876068121721236
49	19.780976592723594	24.72303231785602	30.41160415940415	25.08438693001624
50	19.45299414180405	23.808167464480967	30.06045250099904	26.678385892715937
51	19.66130444763759	23.771606880191815	27.459762101128277	29.107326571042318
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	279.0
1	596.5
2	914.0
3	2461.5
4	4009.0
5	2681.5
6	1354.0
7	1594.0
8	1834.0
9	1681.5
10	1529.0
11	1436.0
12	1343.0
13	1349.5
14	1356.0
15	1314.5
16	1273.0
17	1187.5
18	1102.0
19	1206.0
20	1310.0
21	1510.5
22	1711.0
23	1938.5
24	2166.0
25	2508.5
26	3402.5
27	3954.0
28	4897.5
29	5841.0
30	6798.0
31	7755.0
32	8617.0
33	9479.0
34	10550.5
35	11622.0
36	12461.0
37	13300.0
38	14451.5
39	15603.0
40	17612.5
41	19622.0
42	22361.5
43	25101.0
44	27546.5
45	29992.0
46	38477.5
47	46963.0
48	47781.0
49	48599.0
50	47214.5
51	45830.0
52	39003.0
53	32176.0
54	28887.0
55	25598.0
56	23181.0
57	20764.0
58	19656.0
59	18548.0
60	17792.0
61	17036.0
62	16154.0
63	15272.0
64	12959.5
65	10647.0
66	8662.0
67	6677.0
68	5916.0
69	5155.0
70	4639.0
71	4123.0
72	3569.5
73	3016.0
74	2504.0
75	1622.0
76	1252.0
77	1010.5
78	769.0
79	544.0
80	319.0
81	283.5
82	248.0
83	170.0
84	92.0
85	67.0
86	42.0
87	36.5
88	31.0
89	16.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.64357110235734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.68842628753396	24.118546670207518
2	9.117530694762346	6.1349258441432495
3	3.536128970238318	3.569040193119554
4	2.0261392521363137	2.7266623997010075
5	1.51932898534063	2.5557826373089956
6	1.2709035754215956	2.5654640882361965
7	1.1267720083522288	2.6536043925400934
8	0.97471386160762	2.6234284085959403
9	0.8749281802959487	2.649213759891858
>10	7.7388377612127455	42.441308029557426
>50	0.07044340182871082	1.5812594623106573
>100	0.04950076885260761	3.1381787168568778
>500	0.00253850096680039	0.6379696561415398
>1k	0.0038077514502005854	2.6046157413891042
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2534	0.5386309336552932	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	2383	0.506534141634003	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	2262	0.48081419570965794	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2093	0.4448912960302007	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	1728	0.36730633518403577	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1199	0.2548612823412378	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	944	0.2006580905172047	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	842	0.1789768137875915	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	658	0.13986549105966176	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	544	0.11563347589127052	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT	483	0.10266722216081556	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1256153656483553E-4	0.0	0.0	0.20597212893132563	0.0
2	2.1256153656483553E-4	0.0	0.0	1.1633492896193447	0.0
3	2.1256153656483553E-4	0.0	0.0	1.5555253245814664	0.0
4	2.1256153656483553E-4	0.0	0.0	2.21021485720116	0.0
5	2.1256153656483553E-4	0.0	0.0	4.198515470228632	0.0
6	2.1256153656483553E-4	0.0	0.0	5.247506653176094	0.0
7	2.1256153656483553E-4	0.0	0.0	6.305425420659281	0.0
8	2.1256153656483553E-4	0.0	0.0	7.852235722241589	0.0
9	2.1256153656483553E-4	0.0	0.0	8.473340532084038	0.0
10	2.1256153656483553E-4	0.0	0.0	10.120905001998079	0.0
11	2.1256153656483553E-4	0.0	0.0	11.644120973021689	0.0
12	2.1256153656483553E-4	0.0	0.0	13.198370928383767	0.0
13	2.1256153656483553E-4	0.0	0.0	13.692363939360446	0.0
14	2.1256153656483553E-4	0.0	0.0	13.885369814561315	0.0
15	2.1256153656483553E-4	0.0	0.0	14.270318757280233	0.0
16	2.1256153656483553E-4	0.0	0.0	14.929897205240918	0.0
17	2.1256153656483553E-4	0.0	0.0	15.744433013357368	0.0
18	2.1256153656483553E-4	0.0	0.0	16.67417717429196	0.0
19	2.1256153656483553E-4	0.0	0.0	17.231088400091828	0.0
20	2.1256153656483553E-4	0.0	0.0	17.73804766479896	0.0
21	2.1256153656483553E-4	0.0	0.0	18.351925382398203	0.0
22	2.1256153656483553E-4	0.0	0.0	18.99832501509187	0.0
23	2.1256153656483553E-4	0.0	0.0	19.63303376327447	0.0
24	2.1256153656483553E-4	0.0	0.0	20.133616181884655	0.0
25	2.1256153656483553E-4	0.0	0.0	20.57170550874478	0.0
26	2.1256153656483553E-4	0.0	0.0	20.98917636655812	0.0
27	2.1256153656483553E-4	0.0	0.0	21.416850178126566	0.0
28	2.1256153656483553E-4	0.0	0.0	21.853239012694175	0.0
29	2.1256153656483553E-4	0.0	0.0	22.259869232142705	0.0
30	2.1256153656483553E-4	0.0	0.0	22.753649681582818	0.0
31	2.1256153656483553E-4	0.0	0.0	23.20746856214874	0.0
32	2.1256153656483553E-4	0.0	0.0	23.63599261986345	0.0
33	2.1256153656483553E-4	0.0	0.0	24.036033431678472	0.0
34	4.2512307312967106E-4	0.0	0.0	24.43373606659128	0.0
35	6.376846096945066E-4	0.0	0.0	24.868211847329803	0.0
36	6.376846096945066E-4	0.0	0.0	25.295673097361686	0.0
37	6.376846096945066E-4	0.0	0.0	25.69805208607892	0.0
38	6.376846096945066E-4	0.0	0.0	26.085976890309745	0.0
39	6.376846096945066E-4	0.0	0.0	26.47390169454057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.8380676E-10	45.0	25
CCCTACG	20	7.0290343E-4	45.0	31
CGCATCG	35	1.2098826E-7	45.0	21
CGTTCAT	25	3.887281E-5	45.0	17
TACATCG	20	7.0290343E-4	45.0	21
CGCCTAT	20	7.0290343E-4	45.0	27
CCGTATA	20	7.0290343E-4	45.0	45
AATGACG	20	7.0290343E-4	45.0	1
GAACGCC	20	7.0290343E-4	45.0	24
TTAACGG	20	7.0290343E-4	45.0	2
CCAACGG	25	3.887281E-5	45.0	2
CGCCGAT	20	7.0290343E-4	45.0	40
CAATCGA	25	3.887281E-5	45.0	41
TCGACAG	20	7.0290343E-4	45.0	1
ATTTGCG	45	3.8380676E-10	45.0	12
ATCGACC	20	7.0290343E-4	45.0	24
TACCGGT	20	7.0290343E-4	45.0	40
CGCCAGT	35	1.2098826E-7	45.0	26
TTTAGCG	30	2.1627584E-6	44.999996	1
GCTACGA	65	0.0	41.53846	10
>>END_MODULE